• 한국식물병리학회지
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• 제2권2호
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• pp.69-81
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• 1986

성체식물기에 잎도열병에 대해 저항성인 벼 품종 선발을 위해 한국에서 재배되거나 육종재배로 쓰이고 있는 32개의 벼 품종을 도열병검정 못자리에 공시하였다. 생육시기가 다른 각 잎에 자연감염된 발병율을 토대로 비교할 때, 16개의 통일형 품종들은 모두 고도 저항성이었으나 16개의 일본형 품종들은 잎도열병에 대해 다양한 저항성정도를 나타내었다. 잎도열병에 대한 저항성정도는 품종에 따라 다르지만, 식물체가 성숙해 갈수록 양적저항성정도는 모든 품종에서 증가하였다. 감수성에서 저항성으로 바귀는 엽위는 품종에 따라 달랐다. 도열병균에 대한 품종의 감수성반응이 상위엽에서 저항성반응으로 뚜렷하게 바뀌었다. 잎도열병에 대한 양적 저항성정도가 큰 품종일수록 하위엽에서 저항성반응을 나타내었다. 생육후기에 도열병감염을 급격히 감소시키는 품종인 아까바레, 팔금, 진흥, 올찰, 도봉 및 은하는 포장에서 잎도열병에 대해 성체식물저항성을 보였다. 성체식물저항성품종들은 식물체가 성숙해감에 따라 최적, 질적으로 잎도열병에 대해 저항성을 나타내었다.

• Journal of Crop Science and Biotechnology
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• 제10권3호
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• pp.163-166
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• 2007

Diurnal changes in levels of endogenous gibberellins(GAs) were investigated in three rice cultivars i.e. Sangjubyeo, Shingeumobyeo(photo-neutral) and Chucheongbyeo(photosensitive). The rice cultivars were grown under a 12-hr photoperiod and endogenous GA levels were assayed by gas chromatography-mass spectrometry(GC-MS-SIM) every 3 h for 24 h. The endogenous bioactive $GA_1$ and its immediate precursor $GA_{20}$ contents were significantly different in both photosensitive and photo-neutral rice cultivars, though less pronounced differences were observed for endogenous $GA_{12},\;GA_{53},\;GA_{19}$, and $GA_8$ levels with in the three rice cultivars. The levels of bioactive $GA_1$ and its immediate precursor $GA_{20}$ were significantly higher in Chucheongbyeo than in the other two cultivars. In Chucheongbyeo, the $GA_1$ contents increased significantly from 11.00 to 17.00 o'clock, thus indicating a correlation with light. In Shingeumobyeo, $GA_1$ contents slightly increased during morning hours, while a similar hike in $GA_1$ contents was observed for Sangjubyeo during evening hours. $GA_{19}$ was found to be the most abundant GA form in the three rice cultivars. Our results suggested that GA production in rice depends upon the response potential of rice cultivars and that light positively correlated to GA production in photosensitive rice cultivar.

• Journal of Applied Biological Chemistry
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• 제43권4호
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• pp.197-206
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• 2000

This paper summarizes recent developments in the field of molecular biology and its application to plant breeding, particularly in rice. Plant breeding in the past mostly depended on the time-consuming crossing of known genomes limited to certain traits. Plant breeding has now benefited from marker-assisted selection and genetic engineering to widen the gene pool, improve plant protection, and increase yield. Future plant breeding will expand based on functional and nutritional genomics, in which gene discovery and high-throughput transformation will accelerate crop design and benefits will accrue to human health, in the form of nutritional food for poor people to reduce malnutrition, or food enriched with antioxidants and with high food value for rich people. Agricultural biotechnology for food is no longer a dream but a reality that will dominate the 21st century for agriculture and human welfare.

• Molecules and Cells
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• 제26권4호
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• pp.368-372
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• 2008

Recent molecular and genetic studies in rice, a short-day plant, have elucidated both conservation and divergence of photoperiod pathway genes and their regulators. However, the biological roles of rice genes that act within the autonomous pathway are still largely unknown. In order to better understand the function of the autonomous pathway genes in rice, we conducted molecular genetic analyses of OsFVE, a rice gene homologous to Arabidopsis FVE. OsFVE was found to be ubiquitously expressed in vegetative and reproductive organs. Overexpression of OsFVE could rescue the flowering time phenotype of the Arabidopsis fve mutants by up-regulating expression of the SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) and down-regulating FLOWERING LOCUS C (FLC) expression. These results suggest that there may be a conserved function between OsFVE and FVE in the control of flowering time. However, OsFVE overexpression in the fve mutants did not rescue the flowering time phenotype in in relation to the response to intermittent cold treatment.

• 한국응용곤충학회지
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• 제31권3호
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• pp.256-275
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• 1992

Insects are among the most important abiotic and biotic constraints to rice production. National rice research programs are in various stages in the development and implementation of integrated pest management (IPM) stratagies for rice insect control. Among the various control tactics, insect resistant cultivars are sought as the major tactic in rice IPM. Through the activities of interdisciplinary teams of scientists significant progress has been made in the development and release of insect resistant cultivars to farmers. Because of its compatibility with other control tactics insect resistance has proven to fit well into the IPM approach to rice insect control agents and minimize the need for insecticide applications. The development of biotypes which overcome the resistance in rice plants has been a significant constraint in the breeding of rice for resistance to insects. Most notable examples in Asia are the green leafhopper, Nephotettix virescens, brown planthopper, Nilaparvata lygens and the Asian rice gall midge, Orseolia oryzae. The current breeding stratege is to develop rice cultivars with durable resistance on which virulent biotypes cannot adapt. In spite of the significant progress made in the breeding of insect resistant cultivars there are still numerous important rice insect species for which host plant resistance as a control tactic has not been fully utilized. Advances in biotechnology provide promise of solving some of the problems that have limited the use of host plant resistance as a major tactic in the integrated management of rice insect pests.

• Journal of Plant Biology
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• 제38권4호
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• pp.365-371
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• 1995

Binary vectors, pBI-ActR1, pBI-ActF1 and pBSH-ActR1, were constructed using pGA642, the replication origin of pTi12 and the rice actin promoter. The sizes of pBI-ActR1, pBI-ActF1 and pBSH-ActR1 were 12.9 kb, 13.2 kb and 11.95 kb, respectively. These vectors containing a rice actin promoter followed by a GUS structural gene could induce stronly the expression of GUS gene in transformed rice cells. Rice explants from 3-4 day old seedlings after germinatin were cocultured with A. tumefaceins harboring pBI-ActR1, pBI-ActF1 or pBSH-ActR1, and then GUS expression in the explants was assayed. Transformation of rice explants by these binary vectors was tissue-specific, such that the meristematic regions of shoot apex, root and hypocotyl were transformed by these binary vectors.

• 한국유기농업학회지
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• 제19권spc호
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• pp.135-138
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• 2011

In traditional organic rice culture practices, control of weeds is a big problem. This study was conducted to increase the rice production. Results showed that Plant height, SPAD, root length and weight were higher in pot raised seedling than broadcasting method except number of panicles. High plant density caused reduction in plant height, number of culm and chlorophyll content of the rice. No lodging was observed in both methods. Thickness of third internode and culm length was more in pot raised seedlings at both sites. When organic material was used rice yield increased by 3.81%. Higher rice production (10%) was recorded in the fields planted with pot raised seedlings. From the above study it could be conclude that the pot raised seedlings perform better than the seedlings raised by broadcasting methods.

• The Plant Pathology Journal
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• 제30권2호
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• pp.168-177
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• 2014

Plant has possessed diverse stress signals from outside and maintained its fitness. Out of such plant responses, it is well known that mitogen-activated protein kinase (MAPK) cascade plays important role in wounding and pathogen attack in most dicot plants. However, little is understood about its role in wounding response for the economically important monocot rice plant. In this study, therefore, the involvement of MAPK was investigated to understand the wounding signaling pathway in rice. The OsMPK1 was rapidly activated by wounding within 10 min, and OsMPK1 was also activated by challenge of rice blast fungus. Further analysis revealed that OsMKK4, the upstream kinase of OsMPK1, phosphorylated OsMPK1 by wounding in vivo. Furthermore, OsMPK1 directly interacted with a rice defense-related transcription factor OsWRKY53. To understand a functional link between MAPK and its target transcription factor, we showed that OsMPK1 activated by the constitutively active mutant $OsMKK4^{DD}$ phosphorylated OsWRKY53 in vitro. Taken together, components involving in the wounding signaling pathway, OsMKK4-OsMPK1-OsWRKY53, can be important players in regulating crosstalk between abiotic stress and biotic stress.

• The Plant Pathology Journal
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• 제37권4호
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• pp.375-388
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• 2021

Rice yellow mottle virus (RYMV) is the most harmful virus that affects irrigated and lowland rice in Africa. The RBe24 isolate of the virus is the most pathogenic strain in Benin. A total of 79 genotypes including susceptible IR64 (Oryza sativa) and the resistant TOG5681 (O. glaberrima) as checks were screened for their reactions to RBe24 isolate of RYMV and the effects of silicon on the response of host plants to the virus investigated. The experiment was a three-factor factorial consisting of genotypes, inoculation level (inoculated vs. non-inoculated), and silicon dose (0, 5, and 10 g/plant) applied as CaSiO₃ with two replications and carried out twice in the screen house. Significant differences were observed among the rice genotypes. Fifteen highly resistant and eight resistant genotypes were identified, and these were mainly O. glaberrima. Silicon application did not affect disease incidence and severity at 21 and 42 days after inoculation (DAI); it, however, significantly increased plant height of inoculated (3.6% for 5 g CaSiO₃/plant and 6.3% for 10 g CaSiO₃/plant) and non-inoculated (1.9% for 5 g CaSiO₃/plant and 4.9% for 10 g CaSiO₃/plant) plants at 42 DAI, with a reduction in the number of tillers (12.3% for both 5 and 10 g CaSiO₃/plant) and leaves (26.8% for 5 g CaSiO₃/plant and 28% for 10 g CaSiO₃/plant) under both inoculation treatments. Our results confirm O. glaberrima germplasm as an important source of resistance to RYMV, and critical in developing a comprehensive strategy for the control of RYMV in West Africa.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.119.2-120
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• 2003

Seed-borne fungal pathogens on rice seeds were investigated in order to evaluate their effect on rice grain quality. Rice seeds of two cultivars, Ilmibyeo and Daesanbyeo were collected from 27 areas of Korea and the fungal parasites on seeds were isolated by using a blotter method. Isolation frequency and number of species were varied from collection areas of seed samples. A total 13 species of fungi were identified from the seeds. Among them, Altemana alternata was the most frequent over the country. Bipoiaris oryzae most commonly from Gyeongbuk and Jeonbuk, Alternaria padwickii from Jeonbuk, and Nigrospora oryzae from Choongnam. However Bipolaris. oryzae, Alternaria padwickii, and Nigrospora oryzae were the most frequently isolated fungi from Gyeongbuk and Jeonbuk, and Chungnam, respectively. B. oryzae, A. alternata, A. padwickii, and N. oryzae were dominants on Ilmibyeo showing 10.3％, 10.2％, 5.2％, and 5.2％ infection rate, respectively. While, N. oryzae, A alternata, and Cladosporium sp. were most frequently isolated fungi from Deasanbyeo revealing 15.1％, 9.6％, and 7.5％ infection rate, respectively. These fungi inhabiting on hulls or endosperms of rice seed might be considered as potential factors decreasing rice grain quality. Further investigation of the fungi on grain rice quality are undergoing.