• Journal of Nutrition and Health
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• v.29 no.5
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• pp.507-516
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• 1996

This study was undertaken to estimate seasonal variation of riboflavin status and investigate the relationship between riboflavin intake and its biochemical status in rural women. Dietary intake was measured by determining both 24hr recall method and conventient method. The riboflavin intake was also estimated by food frequency method. Ribofiavin biochemeical status was assessed by erythrocyte glutathione reductase activity coefficient(EGR AC) and urinary riboflavin excretion. All information was repeatly collected in three seasons ; farming season (June), harvest season(October), nonfarming season(February). Mean daily riboflavin intake was below RDA for Koreans in all seasons. Cereal & pulse, vegetable were the primary sources of riboflavin intake and provided above 60% of the total dietary riboflavin intake in all seasons. Riboflavin biochemical status was significantly different among seasons(EGR AC P<0.005, 24hr urinary riboflavin excretion P<0.05), and riboflavin biochemical deficiency was highest in February. The mean values of EGR AC revealed riboflavin deficiency in all seasons. However the mean values of urinary riboflavin excretion were within the normal range except 24hr urinary riboflavin excretion in February. On the basis of EGR AC criteria, 44.7% of subjects were at risk of deficiency in June, 44.7% in October, 81.6% in February. Result of individual riboflavin status assessed by EGR AC or 24hr urinary riboflavin excretion criteria was quite alike. Urinary riboflavin excretion per gram of creatinine underestimated riboflavin deficiency. Significant correlations were observed among biochemical indexes. It also appeared that riboflavin intake measured by food frequency method showed significant correlation with biochemical indexes, especially high correlation with EGR AC (P<0.001). The results indicated that the prevalence of riboflavin deficiency seems to be high among rural women in all seasons and the inadequacy of dietary riboflavin intake was reflected in the abnormalcy of riboflavin biochemical status assessed by EGR, AC. Therefore EGR AC seems to be more sensitive measure of impaired riboflavin status compared to urinary excretion.

• Journal of Nutrition and Health
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• v.29 no.9
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• pp.1003-1012
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• 1996

We examined the relationship among riboflavin intake, work activity, erythrocyte glutathione reductase activity coefficient(EGR AC)and urinary riboflavin excretion. We also attempted to determine factors affecting seasonal riboflavin status of rural women. All information about nutrient intake, work activity and riboflavin biochemical status was repeatly collected in three seasons ; farming season(June), harvest season(October), nonfarming season(February). EGR AC was negatively correlated with riboflavin intake(P<0.005) and positively correlated with the duration(min) of farming activity(P<0.005) and the percentage of lean body mass(LBM) (%) representing long term physical activity(P<0.05) in harvestseason. Urinary riboflavin excretion was positively correlated with the ratio of riboflavin intake to 1,000kcal of energy expenditure (P<0.05) in farming season and negatively correlated with the duration(min) of farming activity (P<0.05) and crude nitrogen balance(P<0.005) in harvest season. It appeared that EGR AC seems to increase and urinary riboflavin excretion seems to decrease as work activity increase. Therefore work activity would be expected to deteriorate riboflavin status. Multiple regression analysis of variables showed that in general EGR AC was affected by riboflavin and energy intakes, energy expenditure, energy balance, the duration(min) of farming activity, LBM (%). Urinary riboflavin excretion was affected by riboflavin and protein intakes, LBM(kg) and crude nitrogen balance. Crude nitrogen balance affected urinary riboflavin excretion in all seasons. The result indicated that work activity as well as nutrient intake seemed to affect riboflavin status, especially EGR AC was affected preferentially be work activity in all seasons.

• Korean Journal of Community Nutrition
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• v.2 no.5
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• pp.701-710
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• 1997

this study was undertaken to compare the riboflavin status of rural women with different physical activity intensity and to determine factors influencing biochemical fiboflavin status. The study was carried out over three different farming seasons : planting (June), harvest(October) and interim(February) in two rural regions of Korea. One was a traditional farming region, the other a commercial farming region with heavier work intensity. Twenty women in the traditional region and eighteen women in the commercial region were involved. The intensity of physical activity was determined by a daily activity record. Body composition was assessed by bioelectrical impedence. Dietary riboflavin intake was measured by the food frequency method. Riboflavin biochemical status was assessed by erythrocyte glutathione reductase activity coefficient (EGR AC) and ruinary riboflavin excretion. The results from the EGR AC and urinary riboflavin excretion during the period showed the overall riboflavin status of the commercial farming women was significantly worse than that of the traditional farming women(EGR AC p<0.0001, urinary riboflavin excretion p<0.05). The traditional farming group had about 40% with risk of riboflavin deficiency, whereas the commercial farming group had about 70%. Overall mean nutrient intake was not significantly different between the two groups, however, overall mean percent lean body mass representing long term physical activity was significantly higher in the commercial farming group ( <0.005). It appears that the biochemical riboflavin status of traditional farming women was significantly influenced by riboflavin intake and crude nitrogen balance while the biochemical riboflavin status of the commercial farming women was significantly influenced by riboflavin intake and percent of lean body mass over the three seasons. (Korean J Community Nutrition 2(5) : 701∼710, 1997)

• Journal of Nutrition and Health
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• v.35 no.9
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• pp.970-981
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• 2002

To assess riboflavin status by dietary intake and urinary excretion of preschool children in Busan and to evaluate the relationship of intakes of food and nutrient with urinary riboflavin excretion, riboflavin food frequencies of 40 common foods affecting intakes of riboflavin by food fequency method, nutrient intake by 24hr recall and 24hr urinary riboflavin excretion were measured with 97 preschool children. The mean riboflavin intake was 0.90 mg and above RDA. Dairy group was the primary source of riboflavin intake and provided 44.8% of the total daily riboflavin intake. The mean urinary riboflavin excretion and riboflavin excretion per gram of creatinine were 395.21$\mu\textrm{g}$ and 2110.41$\mu\textrm{g}$ respectively, The mean riboflavin intake (p<0.01, p<0.01), riboflavin density (p <0.001, p<0.001) and urinary riboflavin excretion per gram of creatinine (p<0.05, p<0.05) were significantly low with the two patterns of food group intake where dairy group was omitted (GMVFDS = 111101, consuming no dairy group and GMVFDS=l11001, consuming no fruit and dairy groups). On the basis of urinary riboflavin excretion per gram of creatinine, 14.3% of subjects in the group aged 1 to 3 and 18.2% of subjects in the group aged 4 to 6 were at risk of deficiency respectively. The urinary riboflavin excretion per gram of creatinine showed positive significant correlations with usual intakes of riboflavin from food groups of dairy (p<0.05), meat (p<0.05) and animal (p<0.05). So nutritional education is needed in order to consume dairy food group daily and to increase usual intake of animal food group including meat.(Korean J Nutrition 35(9) : 970~981, 2002)

• Journal of Nutrition and Health
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• v.25 no.2
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• pp.150-161
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• 1992

The purpose of this study was to evaluate the riboflavin status of primary school children. Fiftyone subjects were selected as obese group and fiftyfive subjects were selected as control group according to Body Mass Index(BMI) of fifth-graders at a primary school in Taegu. For each subject information on nutrient intake and daily activity pattern were obtained by questionnaire. The riboflavin status was evaluated by urinary riboflavin exvretion The daily energy expenditure per kilogram of body weight was significantly lower in obese group(=47kcal/day) than in control group(=58kcal/day) (p<0.001) However the entire energy consumption was siginificantly greater in the obese children(=2005kcal/day) than their nono-baser peers(=1837kcal/day)(p<0.001). Riboflavin intake was 0.67mg/100kcal in the control group and 0.61mg/1000kcal in the obese group. Thus intakes for both groups met the current group and control group were 86.9$\mu\textrm{g}$/day and 98.7$\mu\textrm{g}$/day. repectively. There was no significnat Assesment of clinical signs of riboflavin deficiency indicated that angular lesion was 4.7% and glossitis was 6.6% of all subjects. Thirty one percent of subjects excrete riboflavin below 78$\mu\textrm{g}$/g creatinine which is defined as deficient. Therefore this group would be considered at high risk for developing riboflavin deficiency. From this study current recommendation of 0.6mg/1000kcal of riboflavin intake may not be adequate during growth and associated stress.

• Journal of Nutrition and Health
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• v.27 no.7
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• pp.729-739
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• 1994

Riboflavin status of 17 insulin-dependent diabetic mellitus(IDDM) patients in growing period was evaluated as a function of energy intake and expenditure, biochemical nutritional status and diabetic control indicators. Compared with recommended dietary allowances for Koreans(RDA, 1989), only 35.3% of subjects was at good levels of all nutrients intakes and 52.9% of subjects was below normal level of height and weight. Nutrients consumed below RDA levels were energy(=88.5% of subjects), niacin(64.7%), iron(52.9%) and protein(23.5%) respectively. The riboflavin status was within normal range by urinary riboflavin excretion but 17.6% of subjects was evaluated as showed riboflavin deficiency by erythrocyte glutathione reductase activity coefficient(EGRAC). Correlation between riboflavin intake, urinary riboflavin excretion, EGRA level and diabetic duration were not statistically significant. Correlation analyses indicated that EGRA level was inversely correlated with thiamin, niacin and cabohydrate intake. No significant correlations were found between the EGRA and glycosylated hemoglobin A1(HbA1) (r=-0.464, p=0.129). From this study, it is suggested that IDDM subjects need to maintain balanced diet containing nutrients above RDA for individual activity during growing period. It needs more study whether the current recommended riboflavin allowance is adequate for diabetic patients.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.4
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• pp.594-603
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• 1994

This study investigate the balance and biochemical status of riboflavin in Korean men. During the experimental period, four riboflavin diets with different levels of riboflavin(0.4, 0.6,0.8 and 1.0mg/1000kcal) were followed by eight healthy college men. the riboflavin status was assayed by erythrocyte glutathine reudcctase activity coefficient (EGRAC0 and urinary excretion of riboflavin. Riboflavin intake of the subjects who consumed a det was 0.46mg/1000kcal. the riboflavin intakes of the subjects who consumed the experimental diet with 0.4, 0.6, 0.8 and 1,0mg/1000kcal of riboflavin were 0.41, 0.60, 0.81 , 0.97mg, respectively. Fecal riboflavin loss, absorbed riboflavin , urinary riboflavin loss and retained riboflavin increased in the subjects consumed 0.4, 0.6, 0.8 and 1.0mg/1000kcal of riboflavin. The average EGRAC values for the subjects consumed 0.4, 0.6, 0.8 and 1.0mg/1000kcal of riboflavin were 1.303$\pm$0.029, 1.271$\pm$0.022, 1.239$\pm$0.013, 1.202$\pm$0.030, respectively and urinary riboflavin values ($\mu\textrm{g}$/g creatinine) were 86.89$\pm$ 20.07, 123.88$\pm$ 15.88, 240.70$\pm$57.14 and 393.36$\pm$76.94, respectively. Results indicate that 0.6mg/1000kcal is the level of riboflavin intake needed to maintain urinary riboflavin within the normal range. And above1.0mg/1000kcal of riboflavin is need to maintain urinary riboflavin with in the normal range. And above 1.0mg/1000 of riboflavin is needed to maintain the EGRAC within the normal range. The riboflavin intake correlated positively with urinary riboflavin value, but correlated negatively with the EGRAC value. The EGRAC value correlated negatively to protein intake as well as animal protein intake. The linear equation of between riboflavin intake and EGRAc was EGRA=-0.1667 $\times$riboflavin intake +1.3710. The riboflavin intake to maintain EGRAc below 1.20 was calculated 1.02mg/1000kcal by the above equation.

• Journal of Nutrition and Health
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• v.22 no.6
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• pp.507-515
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• 1989

Ten healthy wome 2-~25 years participated in a 5-week metabolic study to investigate the daily riboflavin requirement of Korean women. Three daily menu and meal patterns were used. Low riboflavin(LR) diet provided riboflavin at a level of 0.71mg / 1000Kcal based on food composition table. High riboflavin(HR) diet provided 1.14mg / 1000Kcal. The riboflavin status was assessed by erythrocyte glutathione reductase activity coefficient(EGRAC) and urinary exretion of riboflavin. Mean EGRAC of LR period and HR period were 1.17+0.08 and 1.01+0.03, repectively. There were discrepanices of riboflavin content of experimental diet between chemical analysis and calculation by Korean food composition table. Urinary riboflavin excretion of subjects was acceptable over the whole study period. It appeared that 0.71mg / 1000Kcal is requirement of riboflavin to maintain EGRAC withing normal range. Thus, it is suggested that current recommendation of 0.6mg / 1000Kcal of riboflavin intake may not be adequate as RDA level in Korea.

• The Korean Journal of Zoology
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• v.38 no.4
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• pp.498-504
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• 1995

The effeds of riboflavin defidency on the biosynthesis of flavin pepddes and levels of flavoenzymes and catecholamines have been investigated. The percentage of 14C. riboflavin radioactivity formed in mitochondria appeared to increase up to 2 weeks but started to decline at 3 weeks. A significant increase of radioactivity incorporation into mitochondria and into trypsin-digestable plus trypsin-non-digestibie flavin peptides was detected in riboflavin-deficient animals. More than 35% of incorporation was observed at the end of the first week and 160% higher incorporation was observed in fiavin peptide after the second week. Activities of MAO and succinate dehydrogenase were affected markedly by riboflavin status whereas those of acetyichoilnesterase were not affected. Riboflavin defidency also brought about marked reductions in levels of epineplrrine and norepinephrine. it is concluded that the levels of flavin peptides, MAO and succinate dehydrogenase, and catecholamines were affected significanily by the availability of riboflavin and in particular the duration of its depiction.

• Journal of Nutrition and Health
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• v.27 no.4
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• pp.368-377
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• 1994

The purpose of this study was to investigate determining factors which influence the riboflavin and pyridoxin nutritional status of korean newborns. The riboflavin and pyridoxine nutriture were evaluated by measuring the enzymecoenzyme saturation kinetics of erythrocyte glutathione reductase and alanine aminotransferase. The subjects were 259 newborns and their mothers. The average activity coefficent values of the cord blood EALAT was 1.4$\pm$0.11 and 32% of the samples had EALATAC values greater than 1.25. EGRAC was 1.26$\pm$0.12 and 27.4 of the blood samples had EALATAC values greater than 1.25. Vitamine B6 nutrition of the newborns whose mothers consumed more energy, protein and vitamine B6 was better as cord blood EALATAC values were found to the lower in this group. A stepwise multiple regression analysis showed that birth length and mother's age significantly influenced EGRAC values, positive and negative ways, respectively.