• Nutrition Research and Practice
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• 제9권1호
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• pp.43-48
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• 2015

BACKGROUND/OBJECTIVES: Physical exercise promotes energy producing pathways requiring thiamin and riboflavin as a coenzyme. Therefore, this study investigated the effects of regular exercise training on urinary excretion of thiamin and riboflavin. MATERIALS/METHODS: Fifty rats were randomly assigned to one of two groups: non-exercise training (NT, n = 25) and regular exercise training (ET, n = 25) for 5 weeks. The rats performed moderate exercise on a treadmill (0.5-0.8 km/hour) for 30 min/day, 5 days/week. Twenty-four hour urine samples were collected at the end of the 0 week, $3^{rd}$ week, and $5^{th}$ week of training and thiamin and riboflavin were analyzed. RESULTS: No significant differences in thiamin and riboflavin intakes for each week were observed between the NT and ET groups. Urinary thiamin excretion of each group was the highest at the $5^{th}$ week compared to the levels at 0 and $3^{rd}$ week. Urinary thiamin at the $5^{th}$ week was significantly lower in the ET group than in the NT group. Urinary riboflavin excretion was increased by training duration, however, no difference was observed between NT and ET for each week. At 0 and $3^{rd}$ week, no significant relationships were observed between dietary intake and urinary excretion of thiamin and riboflavin, however, at the $5^{th}$ week, urinary excretion was significantly increased by dietary intake only in the NT group (P < 0.05). Thiamin excretion of both NT and ET groups was significantly increased with riboflavin excretion at the $5^{th}$ week (P < 0.01). CONCLUSION: Regular moderate exercise training increased urinary excretion of thiamin. Dietary intakes and urinary excretions of thiamin and riboflavin showed positive correlation in both the exercise training and non-exercise training groups as the exercise training period went by, while the correlations in the exercise training group were weaker than those in the non-exercise training group. Therefore, regular exercise training can alter the urinary excretion of thiamin and riboflavin in rats.

• Journal of Microbiology
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• 제38권2호
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• pp.74-79
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• 2000

For the demonstration of a novel riboflavin kinase assay method based on the bacterial bioluminescence, partially purified riboflavin kinase was prepared from bovine liver through ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. Using bacterial luciferase from Photobacterium phosphoreum and the dithionite reduction method, and easy, safe, and fast assay method was established. The optimal temperature, pH, Km values form riboflavin and ATP of boving liver riboflavin kinase determined with this luminescence method were 35$^{\circ}C$, pH 7, 15.3${\mu}$M and 8.3.${\mu}$M, respectively. The detection limit of FMN produced by riboflavin kinase was in the range of 200 pM to 4${\mu}$M which is comparable to the HPLC-fluorescence detection method, while the detection time for each assay was less than 15 sec compared to the HPLC method which requeires at least 10 min for completion.

• 한국식품조리과학회지
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• 제20권1호
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• pp.34-41
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• 2004

The retention rate of riboflavin in two cooked vegetable salads (spinach salad and sesame leaf salad) were examined at every cooking stage, holding temperature and holding time, with various cooking methods, and a better food preparation method developed. The riboflavin contents of the samples were analyzed by HPLC, with fluoresence detection. The changes in the pH and water contents of the samples were measured during the holding stage at various temperatures and times. There were significant differences in the riboflavin contents during the various preparation and cooking processes, such as trimming, washing, boiling and parching etc. The retention rates of the riboflavin with the various holding methods and cooking temperature were also significantly different. The boiling process caused large losses of riboflavin: in the cases of spinach salad and sesame leaf salad these were 78％ and 52％ respectively. The pH and water contents showed faster change during 0-6 hours than during 6-12 hours.

• Journal of Nutrition and Health
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• 제27권7호
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• pp.729-739
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• 1994

Riboflavin status of 17 insulin-dependent diabetic mellitus(IDDM) patients in growing period was evaluated as a function of energy intake and expenditure, biochemical nutritional status and diabetic control indicators. Compared with recommended dietary allowances for Koreans(RDA, 1989), only 35.3% of subjects was at good levels of all nutrients intakes and 52.9% of subjects was below normal level of height and weight. Nutrients consumed below RDA levels were energy(=88.5% of subjects), niacin(64.7%), iron(52.9%) and protein(23.5%) respectively. The riboflavin status was within normal range by urinary riboflavin excretion but 17.6% of subjects was evaluated as showed riboflavin deficiency by erythrocyte glutathione reductase activity coefficient(EGRAC). Correlation between riboflavin intake, urinary riboflavin excretion, EGRA level and diabetic duration were not statistically significant. Correlation analyses indicated that EGRA level was inversely correlated with thiamin, niacin and cabohydrate intake. No significant correlations were found between the EGRA and glycosylated hemoglobin A1(HbA1) (r=-0.464, p=0.129). From this study, it is suggested that IDDM subjects need to maintain balanced diet containing nutrients above RDA for individual activity during growing period. It needs more study whether the current recommended riboflavin allowance is adequate for diabetic patients.

• Mass Spectrometry Letters
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• 제11권1호
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• pp.10-14
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• 2020

Riboflavin is a water-soluble vitamin, which serves as a precursor to flavin mononucleotide and flavin adenine dinucleotide. This study aimed to develop a simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the quantification of riboflavin in the Beagle dog plasma. This method utilized simple protein precipitation with acetonitrile and ¹³C₄, ¹⁵N₂-riboflavin was used as an internal standard (IS). For chromatographic separation, a hydrophilic interaction liquid chromatography (HILIC) column was used with gradient elution. The mobile phase consisted of 0.1% (v/v) aqueous formic acid with 10 mM ammonium formate and acetonitrile with 0.1% (v/v) formic acid. Since riboflavin is an endogenous compound, 4% bovine serum albumin in phosphate buffered saline was used as a surrogate matrix to prepare the calibration curve. The quantification limit for riboflavin in the Beagle dog plasma was 5 ng/mL. The method was fully validated for its specificity, sensitivity, accuracy and precision, recovery, and stability according to the US FDA guidance. The developed LC-MS/MS method may be useful for the in vivo pharmacokinetic studies of riboflavin.

• 한국미생물·생명공학회지
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• 제15권2호
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• pp.112-115
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• 1987

B. megaterium ATCC 13639와 E. aerogenges의 비타민 B$_{12}$ 생산을 위한 최적 pH는 각각 6.0과 5.0으로 pH의 영향을 크게 받았으나 두 세균에 의한 riboflavin 생산은 pH에 따른 변화가 거의 없었다. Sucrose를 첨가하면 두 비타민 생산량이 크게 증가되었고 최적pH도 변함을 알 순 있었다. 두 세균에 의한 두 비타민 생산에 미치는 온도의 영향은 pH에 비하여 아주 적었다. 이상의 결론은 장차 여러 가지 식품 폐기물을 이용한 비타민 B$_{12}$와 riboflavin의 생산이 가능함을 보여주었다.

• 한국미생물·생명공학회지
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• 제21권6호
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• pp.615-621
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• 1993

In order to maximize the riboflavin production by a mutant strain Ashbya gosspyii, the optimization of medium and fed-batch fermentation were performed. As carbon sources, glucose and soybean oil were necessary for the riboflavin overproduction. Optimal concentrations of glucose and soybean oil in the flask cultures were found to be 3.0% and 0.5%, respectively, in a complex medium containing corn steep liquor(CLS) 1%. Among the various organic nitrogen sources tested, CSL was the most effective one both for the cell growth and riboflavin overproduction.

• Archives of Pharmacal Research
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• 제8권3호
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• pp.99-107
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• 1985

The interaction of reduced riboflavin 2', 3', 4', 5'-tetrabutyrate with salicylic acid, aspirin, and salicylamide has been spectroscopically investigated to determine the binding mechanism. Hydrogen-1 and carbon-13 unclear magnetic resonance, infrared, and absoption spectra were measured in chloform-d and chloroform. The association of the reduced riboflavin with salicylic acid derivatives is different from that osidizd one. Salicylic acid and the reduced riboflavin form a cyclic hydrogen bounded complex through the imino (3-N, 5-N) protons and the carbonyl (2-C, 4-C) oxygens of the isolloxazine ring of the latter, and the carboxylic hydroxyl proton and carbonyl oxygen of the former. Aspirin and the reduced riboflavin form a complex by the same mode as salicylic acid. Salicylamide forms a cyclic hydrogen bonded complex with the reduced riboflavin through the imino (3-N, 5-N) protons and the carbonyl (2-C, 4-C) oxygens of the isoalloxazine ring, and the amino proton and the carbonyl oxygen of salic aylmide. It appears that both the oxidized and reduced form of riboflavin are associated with salicylic acid derivatives.

• Applied Biological Chemistry
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• 제13권3호
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• pp.219-222
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• 1970

탁주 양조에 사용되는 각종 원료중의 Riboflavin을 정량하고 아울러 쌀, 소맥분및 옥수수분을 각각 주원료로 일반 탁주양조법에 따라 담금하여 숙성시까지 그들 술덧중의 Riboflavin의 소장을 측정하여 다음과 같은 결과를 얻었다. 1. 각원료 100g중의 Riboflavin함량 (${\gamma}$)은 쌀 46.9, 소맥분 67.3, 옥수수분 73.9, 누룩 169.0, 소맥분입곡 87.1, 옥수수분입곡 $84.4{\gamma}$이었으며 쌀소맥분 및 옥수수분중의 함량은 증자에 의하여 약 $30{\sim}40%$파괴됨을 보였다. 2. 누룩단용법의 쌀및 소맥분구의 각술덧에서 Riboflavin의 소장은 같은 경향을 보여 3일까지 감소하였다가 이후 다시 증가하며 5일째에 가서 급격히 증가하여 6일째에 약간 감소하는 소장을 보여 결국 양 숙성술덧 100ml중의 Riboflavin함량은 누룩단용법의 쌀구에서 $82{\gamma}$, 소맥분구에서 $72{\gamma}$를 보였으며 누룩및 입곡혼용법의 소맥분및 옥수수분구의 각술및에서는 초기부터 감소하지 않고 기복이 있는 증가의 경향을 보이다가 역시 5일째에 현저히 증가되며 6일째에 다시 감소하여 결국 양 숙성술덧 100ml중에는 소맥분구에서 $88.6{\gamma}$ 옥수수분구에서 $83.6{\gamma}$이었다. 3. 각숙성술덧으로 제성한 탁주 100ml중에는 Riboflavin 이 누룩단용법구들에서 $28.8{\sim}41{\gamma}$, 누룩및 입곡혼용법구들에서 $33.6{\sim}44.5{\gamma}$ .함유됨을 알 수 있었다. 5. 누룩단용법의 쌀구및 소맥분구의 지게미 무수물 100g중에는 Riboflavin이 각각 $47.3{\gamma}$및 $66.2{\gamma}$함유되었으며 누룩및 입곡혼용법의 소맥분구및 옥수수분구의 것에는 각각 $64.5{\gamma}$ 및 $62.9{\gamma}$ 함유되었다.

• 대한수의학회지
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• 제41권4호
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• pp.591-596
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• 2001

The chicks from 6 field broiler farms revealed peripheral neuropathy including leg weakness, curled toes and drooped wings. Grossly distinctive enlargements of sciatic nerve, branchial nerve and lumbar nerve were observed in the chicks. Histologically nerve lesions consisted of demyelination of myelin sheaths, Schwann cell proliferation and swelling, and interstitial edema in the peripheral nerves of all birds examined. Axonal swelling and infiltration of small lymphocytes were observed, but not a primary lesion. After treatment of riboflavin, neurological disorder was markedly recovered. From these results, it is suggested that the peripheral nerve lesions in these cases were caused by dietary riboflavin deficiency.