• Korean Journal of Microbiology
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• v.27 no.3
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• pp.285-290
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• 1989

The biololgical effect of the preemergence rice field herbicide, butachlor(commercial name, Machete) on purple nonsulfur photosynthetic bacterium Rhodospirillum rubrum KS-301 has been studied under cultural conditions. Bacterial growth showed a tendency to decline according to the degree of the concentration of butachlor until $10^{-3}$ M and slmost stopped at $10^{-2}$M. The growth inhibitory action at $10^{-3}$M of butachlor was evident (4.2-18.7% inhibition of growth rate) but had little effect in nitrogen fixation. Conversely, there was a little enhancement effect(1%) in pyruvate, dinitrogen gas growing cultures. At concentration of $10^{-3}$ M, instead of spiral form, rod shapes were observed through phase contrast microscope and instead of vesicular intracytoplasmic membrane, irregular tubular forms were observed through electron microscope. Alkaline pH value slightly reversed tha inhibitory action of butachlor.

• Microbiology and Biotechnology Letters
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• v.16 no.6
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• pp.510-516
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• 1988

The effects of input substrate concentration and dilution rate on mass transfer resistance in the operation of immobilized cell reactors were investigated using Rhodospirillum rubrum KS-301 immobilized by Ca alginate as reactor element and glucose as growth-limiting substrate. The kinetic parameters were obtained to estimate effectiveness factors. In the packed-bed reactor, internal mass transfer resistance was predominating although external resistance could not be neglected. The overall effectiveness factor was decreased with increase of dilution rate. In the continuous stirred-tank reactor, external resistance was nearly neglected and the overall effectiveness factor was not affected by dilution rate. In this experiment the overall effectiveness factors in PBR and CSTR were estimated to be 0.70 and 0.71 at D$_{i}$ = 0.2/h, R = 0.15 cm, and S$_{i}$ : 1.0g/L, respectively.

• Journal of the Korean Society of Radiology
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• v.12 no.3
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• pp.381-387
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• 2018

A Effect on physiological metabolism of microorganism which irradiated by visible light of non-ionization radiation(12,000 Lux) was investigated. The microorganism used in this experiment was a Rhodospirillum rubrum KS-301 of chemosynthetic microorganism. Batch fermentation of glucose were implement, and based on the data resulted from the fermentation. First, physiological characteristic of microorganism which was not irradiated was investigated. As a result, the decrease of the residual quantity of the substance(5.03 g/L - 2.17 g/L) was increased with the quantity of the bacteria(1.08 g/L - 3.14 g/L)and the quantity of the hydrogenous production(0.186 g - 0.3 g) respectively. Second, physiological characteristic of microorganism which was irradiated was investigated. As a result, the decrease of the residual quantity of the substance(13.17 g/L - 5.2 g/L) was increased with the quantity of the bacteria(4.7 g/L - 10.57 g/L)and the quantity of the hydrogenous production(0.186 g - 0.3 g) respectively. As the physiological characteristic of microorganism which was irradiated by visible light of non-ionization radiation(12,000 Lux) was active with its life, but the cell damages irradiated by with gamma-ray, X-ray, electron-ray in ionization radiation were appeared at cell.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.303-309
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• 1985

Continuous production of hydrogen by Ca alginate-immobilized photosynthetic bacteria was studied in a packed-bed bioreactor. The dilution rate and input concentration of carbonaces substrate were selected as operating parameters. To choose the strain for immobilization, hydrogen productivities of Rhodopseudomonas caposulata 10006 and Rhodospirillum rubrum KS-301 were compared through preliminary batch cultures of their free cells: the former was found to show better hydrogen productivity in spite of its lower specific growth rate. For the continuous production of hydrogen by immobilized R capsulata, the optimum dilution rate was about 0.84 h$^{-1}$ . The Immobilized tells gave better hydrogen yield and conversion efficiency than free ones. And a kinetic parameter K'$_{m}$ was determined for the packed-bed bioreactor, being practically constant for a specific range of dilution rates.s.