• Korean Journal of Materials Research
• /
• v.34 no.2
• /
• pp.105-110
• /
• 2024

Scanning probe microscopy (SPM) has become an indispensable tool in efforts to develop the next generation of nanoelectronic devices, given its achievable nanometer spatial resolution and highly versatile ability to measure a variety of properties. Recently a new scanning probe microscope was developed to overcome the tip degradation problem of the classic SPM. The main advantage of this new method, called Reverse tip sample (RTS) SPM, is that a single tip can be replaced by a chip containing hundreds to thousands of tips. Generally for use in RTS SPM, pyramid-shaped diamond tips are made by molding on a silicon substrate. Combining RTS SPM with Scanning spreading resistance microscopy (SSRM) using the diamond tip offers the potential to perform 3D profiling of semiconductor materials. However, damage frequently occurs to the completed tips because of the complex manufacturing process. In this work, we design, fabricate, and evaluate an RTS tip chip prototype to simplify the complex manufacturing process, prevent tip damage, and shorten manufacturing time.

• Structural Engineering and Mechanics
• /
• v.89 no.5
• /
• pp.467-477
• /
• 2024

Manufacturing industries, now-a-days, focus mostly on redesigning of the products for reducing cost and lead-time via detailed analysis of its composition and constructional design regarded as the Reverse Engineering (RE) process that involves the acquisition of relevant data of the original product, analysis for its functional use and finally, reproduction of the design for improving the functionality. In the present work, a new model based on optimization at different steps of RE, is proposed to redesign a structural component, which is subjected to severe tensile stress while in service. The component under study is an accessory namely, hitch bracket, attached to the rear axle of a tractor to connect it to the plough. The methodology includes building of a 3D Computer Aided Design (CAD) model from the scanned data of the existing component with the help of 3D scanner. Computer Aided Engineering (CAE) analysis is carried out on the CAD model with existing load conditions by Finite Element Analysis (FEA). Topological optimization is carried out giving rise to a modified/optimized design of the component. It is observed that the performance of the modified component improves significantly with simultaneous weight reduction without affecting its functional use and the manufacturing process setup.

• The Korean Journal of Applied Statistics
• /
• v.26 no.3
• /
• pp.531-547
• /
• 2013

Multiple life models are useful in multiple life insurance and multiple life annuities when the payment times of benets in these insurance products are contingent on the future life times of at least two people. A reverse mortgage is an annuity whose monthly payments terminate at the death time of the last survivor; however, actuaries have used female life table to calculate monthly payments of a reverse mortgage. This approach may overestimate monthly payments. This paper suggests a last-survivor life table rather than a female life table to avoid the overestimation of monthly payments. Next, this paper derives the distribution of the future life time of last survivor, and calculates the expected life times of male, female and last survivor. This paper calculates principal limits and monthly payments in cases of male life table, female life table and last-survivor life table, respectively. Some numerical examples are discussed.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.4
• /
• pp.816-824
• /
• 2017

Human noroviruses are widespread and contagious viruses causing nonbacterial gastroenteritis. Real-time reverse transcription quantitative PCR (real-time RT-qPCR) is currently the gold standard for the sensitive and accurate detection of these pathogens and serves as a critical tool in outbreak prevention and control. Different surveillance teams, however, may use different assays, and variability in specimen conditions may lead to disagreement in results. Furthermore, the norovirus genome is highly variable and continuously evolving. These issues necessitate the re-examination of the real-time RT-qPCR's robustness in the context of accurate detection as well as the investigation of practical strategies to enhance assay performance. Four widely referenced real-time RT-qPCR assays (Assays A-D) were simultaneously performed to evaluate characteristics such as PCR efficiency, detection limit, and sensitivity and specificity with RT-PCR, and to assess the most accurate method for detecting norovirus genogroups I and II. Overall, Assay D was evaluated to be the most precise and accurate assay in this study. A ZEN internal quencher, which decreases nonspecific fluorescence during the PCR, was added to Assay D's probe, which further improved the assay performance. This study compared several detection assays for noroviruses, and an improvement strategy based on such comparisons provided useful characterizations of a highly optimized real-time RT-qPCR assay for norovirus detection.

• Korean Journal of Veterinary Service
• /
• v.38 no.2
• /
• pp.107-116
• /
• 2015

In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that accidentally infected in pig population. The RT-LAMP was completed in 40 min at $58^{\circ}C$ and the sensitivity of the RT-LAMP ($1copy/{\mu}L$) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) ($10copy/{\mu}L$) and the same to real time RT-PCR ($1copy/{\mu}L$). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diagnostic laboratory in the future.

• Membrane Journal
• /
• v.8 no.1
• /
• pp.29-41
• /
• 1998

Thin-film composite reverse osmosis membranes of aromatic polyamides were prepared by the interfacial polymerization. Aromatic polyamides as active skin layer were made from the interfacial polymerization of MPD(m-phenylene diamine) in the aqueous and TMC(trimesoyl chloride) in HCFC(1,1-dichloro-1-fluoroethane) organic solvent. The performances of the various reverse osmosis composite membranes prepared by changing processing variables were examined. The performance of membrane manufactured by batch system was varied with organic solvent, monomer concentration, dipping time, heat treatment temperature, acid acceptor, ethanol post treatment, and acid post treatment. Ethanol post treatment was the most dominant factors in increasing permeate amount, while the monomer concentration and dipping time were the main factors in increasing selectivity. The spiral-wound module was produced with the membrane prepared at optimum condition of the continuous process. Comparing the performance of this membrane module made here with that of commercial membrane module, the permeate flux was increased by 33% while the rejection was decreased by 5%.

• Journal of KIISE:Information Networking
• /
• v.32 no.3
• /
• pp.359-369
• /
• 2005

Delay estimation is a difficult Problem in computer networks. Accurate one-way delay estimation is crucial because it serves a very important role in network performance and thus application design. RTT(Round Trip Time) is often used as an approximation of the delay, but because it is a sum of the forward and reverse delays, the actual one-way delay cannot be estimated accurately from RTT. To estimate one-way delay accurately, this paper proposes a new scheme that analytically derives one-way delay, forward and reverse delay respectively. We show that the performance of TCP can improve dramatically in asymmetric networks using our scheme. A key contribution of this paper is that our one-way deiay estimation is much more accurate than RTT estimation so that TCP can quickly find the network capacity in the slow start phase. Since RTT is the sum of the forward and reverse delays, our scheme can be applied to any protocol that is based on RTT.

• Genomics & Informatics
• /
• v.18 no.4
• /
• pp.40.1-40.8
• /
• 2020

Avian influenza (AIV) outbreaks can induce fatal human pulmonary infections in addition to economic losses to the poultry industry. In this study, we aimed to develop a rapid and sensitive point-of-care AIV test using loop-mediated isothermal amplification (LAMP) technology. We designed three sets of reverse transcription LAMP (RT-LAMP) primers targeting the matrix (M) and hemagglutinin (HA) genes of the H5 and H9 subtypes. RT-LAMP targeting the universal M gene was designed to screen for the presence of AIV and RT-LAMP assays targeting H5-HA and H9-HA were designed to discriminate between the H5 and H9 subtypes. All three RT-LAMP assays showed specific amplification results without nonspecific reactions. In terms of sensitivity, the detection limits of our RT-LAMP assays were 100 to 1,000 RNA copies per reaction, which were 10 times more sensitive than the detection limits of the reference reverse-transcription polymerase chain reaction (RT-PCR) (1,000 to 10,000 RNA copies per reaction). The reaction time of our RT-LAMP assays was less than 30 min, which was approximately four times quicker than that of conventional RT-PCR. Altogether, these assays successfully detected the existence of AIV and discriminated between the H5 or H9 subtypes with higher sensitivity and less time than the conventional RT-PCR assay.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
• /
• 2005.05a
• /
• pp.228-230
• /
• 2005

Magnetic field has been used to hold plasma at high temperature for a long time. Reverse field has shown unstable process. Using ratio frequency, the author could control the instability of the process and formed a stable erversed field.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.8A
• /
• pp.898-908
• /
• 2004

In this paper, we propose a high speed preamble searcher feasible for RACH(Random Access Channel) preamble structure in WCDMA reverse link receiver. Unlike IS-95, WCDMA system uses AISMA(Acquisition Indication Sense Multiple Access) process. Because of the time limit between RACH preamble transmission and AI(Acquisition Indicators), and the restriction on the number of RACH signatures assigned to RACH preamble, fast acquisition indication is required for efficient operation. The preamble searcher proposed in this paper is based on 2-antenna system and has adopted FHT algorithm that has the radix-2 16 point FFT structure. The acquisition speed using FHT is 64 times faster than the conventional method that correlates each signature. Based on their fast aquisition scheme, we improved the acquisition performance by calculating the correlation up to the 4096 chips of the total preamble length. The performance is analyzed by using Neyman-pearson method. The proposed algorithm has been applied for the implementation of WCDMA reverse link receiver modem successfully.