• Journal of Photoscience
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• v.1 no.2
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• pp.95-105
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• 1994

Experiments were carried out to investigate whether P, deficiency in detached 25 mM mannose-feeding led to a decline of the photosynthetic electron transport rates through acidification of the thylakoid lumen. With increasing mannose-feeding time, the maximal CO2 exchange rates and the maximal quantum yields of photosynthesis decreased rapidly up to 6 h by 73% then with little decrease up to 12 h. The ATP/ADP ratio declined by 54% 6 h after the treatment and then recovered to the control level at 12 h. However, the NADPH/NADP~ ratio was not significantly altered by mannose treatment. Electron transport rates of thylakoid membranes isolated from 6 h treated leaves did not change, but they decreased by 30% in 12 h treated leaves. The quenching analysis of Chl fluorescence in mannose-treated leaves revealed that both the fraction of reduced plastoquinone and the degree of acidification of thylakoid lumen remained higher than those of the control. The reduction of PSI in mannose fed leaves was inhibited due to acidification of thylakoid lumen (high qE). The reduction of primary quinone acceptor of PSII was inhibited by mannose feeding. Mannose treatment decreased the efficiency of excitation energy capture by PSII. Fo quenching was induced when treated with mannose more than 6 h, and had a reverse linear correlation with (Fv)m/Fm ratio. These results suggest that Pi deficiency in Chinese cabbage leaves reduce photosynthetic electron transport rates by diminishing both PSII function and electron transfer from PSII to PSI through acidification ofthylakoid lumen, which in turn induce the modification of photosynthetic apparatus probably through protein (de)phosphorylation.

• Archives of Reconstructive Microsurgery
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• v.16 no.2
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• pp.86-92
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• 2007

Failure of reattachment of finger is inevitable in replantation surgery and that failure rate is about 10 % are reported in many authors. Management of the failed finger replantation is challenge to microsurgeons. We report 7 cases of thumb reconstruction after failure of replantation. The reconstructive surgery composed with early debridement of soft tissue that are under gangrenous processing, extract the phalangeal bone without any soft tissues. Osteosynthesis of the extracted phalangeal bone with host phalangeal bone. The exposed bony portion covered with vascularized flaps such as reverse radial forearm pedicled flap, free radial forearm flap and neurovascular island finger flap. This procedure underwent within a week after vascular insufficiency developed. All of the flaps are survived, bone union achieved within 3 months. The function and external appearance of the reconstructed thumb were encouraging; pinch power was average 1.2 pounds. Early removal of necrotizing soft tissue followed by covering none vascular phalangeal bone which extracted from the dead phalanx with vascularized flap is one of the useful alterative solutions in failed replantation surgery in hand.

• Journal of the Institute of Convergence Signal Processing
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• v.1 no.1
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• pp.49-57
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• 2000

In this study, a new algorithm for canceling a MRI artifact due to the translational motion In the image plane is described. Unlike the conventional iterative phase retrieval algorithm, in which there is no guarantee for the convergence, a direct method for estimating the motion is presented. In previous approaches, the motions in the x(read out) direction and the y(phase encoding) direction were estimated simultaneously. However, the feature of x and y directional motions are different from each other. By analyzing their features, each x and y directional motion is canceled by the different algorithms in two steps. First, it is noticed that the x directional motion corresponds to a shift of the x directional spectrum of the MRI signal, and the non-zero area of the spectrum just corresponds to the projected area of the density function on the x axis. So the motion is estimated by tracing the edges between non-zero area and zero area of the spectrum, and the x directional motion is canceled by shifting the spectrum in an reverse direction. Next, the y directional motion is canceled by using a new constraint condition, with which the motion component and the true image component can be separated. This algorithm is shown to be effective by using a phantom image with simulated motion.

• Animal cells and systems
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• v.13 no.4
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• pp.381-389
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• 2009

Using the DDRT-PCR, a series of differentially expressed genes in human primary cervical cancer was isolated. Among the 250 PCR amplimers, 88 gene fragments were confirmed by reverse Northern hybridization. Homology searches indicated that 26 out of 88 were previously known genes including calmodulin, human BBC1, histone H3.3, a series of ribosomal proteins (RPL19, RPS19, and RPS12), translation initiation factor (eIF-4AI), lactoferrin, integrin ${\alpha}6$, cell-surface antigens (CD9 and CD59), transcription factor (mbp-1), and mitochondrial proteins. Several unknown clones showed sequence homology with known genes. Furthermore, six of the unknown genes showed identical sequence with expressed sequence tags (EST) of unknown function. Differential expression patterns of identified genes were further examined and confirmed with multiple pairs of cervical cancer samples using Northern hybridization. Our profiling of differentially expressed genes may provide useful information about the underlying genetic alterations in human cervical carcinoma and diagnostic markers for this disease. The precise roles of these genes in cancer development remain to be elucidated.

• The Journal of the Acoustical Society of Korea
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• v.24 no.3E
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• pp.87-89
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• 2005

The acoustical sources of intake and exhaust systems in fluid machines are often characterized by the source impedance and strength using linear frequency-domain modeling. In the case of the sources which are nonlinear and time-variant, however, the source parameters were sometimes incorrectly obtained. In this paper, the source model and direct measurement technique are modified in order to evaluate the effect due to nonlinear and periodically time-varying source character as well as the linear property of the reflectivity of in-duct fluid machine source. With a priori known kinematical information of the source, the types of nonlinear time-variant terms can be presumed by a simple physical model, in which there is practically no restriction on the form of the model. The concept of source impedance can be extendable by introducing the linear frequency response function for each nonlinear or time-variant input. Extending the conventional method and adapting the reverse MISO technique, it is possible to develop a direct method that can deal with the nonlinear time-variant source parameters. The proposed direct method has a novel feature that there is no restriction on the probability or spectral natures of the excited sound pressure data. The present method is verified by the simulated measurements for simplified fluid machines. It is thought that the proposed method would be useful in predicting the insertion loss or the radiated sound level from intake or exhaust systems.

• Molecules and Cells
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• v.38 no.11
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• pp.959-965
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• 2015

Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.

• 한국연소학회:학술대회논문집
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• 1998.10a
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• pp.9-21
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• 1998

The optimization of frontal device including fuel nozzle and swirler is required to secure the mixing of fuel and air, and the combustion stability in the gas turbine combustor design for the reduction of pollutant emissions and the increase of combustion efficiency. The effects of injection nozzle and swirler on the flow field, spray characteristics and consequently the combustion stability, were experimentally investigated by measuring the velocity field, droplet sizes of fuel spray, lean combustion limit and the temperature field in the main combustion region. The effect of fuel injection nozzle was tested by adopting three different nozzles; a dual orifice fuel nozzle, a hollow cone nozzle and a solid cone nozzle. These tests were combined with the three different swirler geometries; a dual-stage swirler with 40$^{\circ}$ /-4 5$^{\circ}$ vanes and two single-stage swirlers with 40$^{\circ}$ vane angle having 12 and 16vanes, respectively. Flow fields and spray characteristics were measured with APV(Adaptive Phase Doppler Velocimetry) under atmospheric condition using kerosine fuel. Temperatures were measured by Pt-PtI3%Rh, R-type thermocouple which was 0.2mm thick. It was found that the dual swirler resulted in the biggest recirculation zone with the highest reverse flow velocity at the central region, which lead the most stable combustion. The various combustion characteristics were observed as a function of the combination between the injector and swirler, that gave a tip for the better design of gas turbine combustor.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.36S no.9
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• pp.18-26
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• 1999

Recently, multiuser detection has been investigated as an interesting topic because of its capability of eliminating multiuser interference and resistance to the near-far problem. In this paper, we consider a quasi maximum likelihood detector in the reverse link system which used antenna arrays in quasi-synchronous channels. It is also shown that the proposed system can suppress the interuser interference without using the side information of the other users. It is also shown that the performance of the proposed system is superior to that of the conventional matched filter system.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.8 no.6
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• pp.645-655
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• 1997

In this paper, the performance of the noncoherent code tracking loop designed at baseband for CDMA applications is analyzed in detail and is confirmed by computer simulations. Analytical closed-form formula for jitter variance is derived for AWGN channel environments as a function of pulse shaping filter, timing offset, signal-to-interference ratio, and loop filter coefficients. The design issue of the loop filter is also addressed with emphasis on the second-order tracking loop. Finally, the performance of the designed tracking loop is examined by computer simulations for both AWGN and Rayleigh fading channels, when applied to the reverse link of the coherent CDMA system for IMT-2000 designed by ETRI.

• International Journal of Oral Biology
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• v.31 no.4
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• pp.135-140
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• 2006

Temperature signaling can be initiated by members of transient receptor potential (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. Since odontoblasts constitute a well-defined layer between the pulp and the mineralized dentin, being first to encounter thermal stimulation from oral cavity, they may be involved in sensory transduction process, in addition to their primary function as formation of dentin. We investigated whether thermo-TRP channels are expressed in a odontoblast cell line, MDPC-23. The expressions of thermo-TRP channels were examined using reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, fluorometric calcium imaging. Analysis of RT-PCR revealed mRNA expression of TRPV1, TRPV2, TRPV4 and TRPM8, but no TRPV3, TRPA1. Immunohistochemical approach failed to detect TRPV1 expression. Whereas the application of 4-phorbol-12,13-didecanoate($10\;{\mu}M$, a TRPV4 agonist), menthol(1 mM, a TRPM8 agonist) and icilin($10\;{\mu}M$, a TRPM8 agonist) produced the enhancement of intracellular calcium concentration, capsaicin($1\;{\mu}M$, a TRPV1 agonist) did not. Our results suggest that subfamily of thermo-TRP channels expressed in odontoblasts may serve as thermal or mechanical transducer in teeth.