• Journal of Plant Biology
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• v.16 no.1_2
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• pp.12-16
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• 1973

The inhibitory effect of Inula sesquiterpene lactones on the growth of Phaseolus vulgaris was tested and the abnormality of the stem organization caused by the lactones was also examined. The longitudinal growth of the young stem and the expansion of the young leaf were stopped by the application of the lactones. However, this inhibitory effect was appeared and strictly restricted within the treated area. So the young shoot was observed for possible bending as a result of the unilateral application of the lactones. When the application of the lactones into the medium, the growth of the plant was entirely repressed. However, the growth of shoot and re-initiation of root were started after the plant was transfered to the lactone free medium. And partial reversal of inhibition of the stem growth was achieved by the additions of gibberelline and the lactones.

• Journal of Magnetics
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• v.7 no.1
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• pp.4-8
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• 2002

The magnetoresistance (MR) and the magnetization reversal of a lateral spin-injection device based on a spin-polarized field effect transistor (spin FET) have been investigated. The device consists of a two-dimensional electron gas (2DEG) system in an InAs single quantum well (SQW) and two ferromagnetic $(Ni_{80}Fe_{20})$ contacts: all injector (source) and a detector (drain). Spin-polarized electrons are injected from the first contact and, after propagating through the InAs SQW are collected by the second contact. By engineering the shape of the permalloy contacts, we were able to observe distinct switching fields $(H_c)$ from the injector and the collector by using scanning Kerr microscopy and MR measurements. Magneto-optic Kerr effect (MOKE) hysteresis loops demonstrate that there is a range of magnetic field (20~60 Oe), at room temperature, over which the magnetization in one contact is aligned antiparallel to that in the other. The MOKE results are consistent with the variation of the magnetoresistance in the spin-injection device.

• The Korean Journal of Pain
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• v.18 no.2
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• pp.107-112
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• 2005

Background: Nerve ligation injury may produce mechanical allodynia, but this can be reversed after an intrathecal administration of adenosine analogues. In many animal and human studies, ATP-sensitive potassium channel blockers have been known to reverse the antinociceptive effect of various drugs. This study was performed to evaluate the mechanical antiallodynic effects of spinal R-PIA (Adenosine A1 receptor agonist) and the reversal of these effects due to pretreatment with glibenclamide (ATP-sensitive potassium channel blocker). Thus, the relationship between the antiallodynic effects of R-PIA and ATP-sensitive potassium channel were investigated in a neuropathic model. Methods: Male Sprague Dawley rats were prepared by tightly ligating the left lumbar 5th and 6th spinal nerves and implantation of a chronic lumbar intrathecal catheter for drug administration. The mechanical allodynia was measured by applying von Frey filaments ipsilateral to the lesioned hind paw. And the thresholds for paw withdrawal assessed. In study 1, either R-PIA (0.5, 1 and $2{\mu}g$) or saline were administered intrathecally for the examination of the antiallodynic effect of R-PIA. In study 2, glibenclamide (2, 5, 10 and 20 nM) was administered intrathecally 5 min prior to an R-PIA injection for investigation of the reversal of the antiallodynic effects of R-PIA. Results: The antiallodynic effect of R-PIA was produced in a dose dependent manner. In study 1, the paw withdrawal threshold was significantly increased with $2{\mu}g$ R-PIA (P < 0.05). In study 2, the paw withdrawal threshold with $2{\mu}g$ R-PIA was significantly decreased almost dose dependently by intrathecal pretreatment of 5, 10 and 20 nM glibenclamide (P < 0.05). Conclusions: These results demonstrated that an intrathecal injection of ATP-sensitive potassium channel blockers prior to an intrathecal injection of adenosine A1 receptors agonist had an antagonistic effect on R-PIA induced antiallodynia. The results suggest that the mechanism of mechanical antiallodynia, as induced by an intrathecal injection of R-PIA, may involve the ATP-sensitive potassium channel at both the spinal and supraspinal level in a rat nerve ligation injury model.

• Economic and Environmental Geology
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• v.14 no.3
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• pp.111-122
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• 1981

This study is a model analysis for an effective application of the geophysical prospecting to the investigation of geological structures or useful resources, and the purpose of it is to research a property of the electrical resistivity prospecting, especially by using a Three-Point electrode array method. In using the Three-Point electrode array method, it is theoretically assumed to choose the infinite for a distance between the two current electrodes, however it is impossible in applying to the practical field prospecting. Therefore this study was conducted for determination and presentation of a minimum appropriate distance between the two current electrodes by making a study on prospecting effect in the variation of distance between both the electrodes. In case that the ratios of the distance between the two current electrodes to that between the two potential electrodes are respectively chosen for 40, 400, 5,000, the experimental data of this study showed that the minimum appropriate distance between the two current electrodes is forty times as much as that between two potential electrodes. In order to make clear a problem about prospecting depth which is essential to the data processing, it had been chosen equally to the distance between two potential electrodes. As a result of it, it was shown that the anomaly is appeared along the position of an assumed ore body. Consequently it was found out that the prospecting depth of the Three-Point electrode array method is the same as the distance between the two potential electrodes. From the model experiment on the sheeting ore body(or linear structure) of horizontal, dipping of $30^{\circ}$, $60^{\circ}$ and vertical on the basis of above experimental condition, it was found out that the position and dip of assumed ore body could be inferred from the aspects of the equiresistivity curve. In consequence of performing out the simultaneous Normal and Reversal electrode movement, it was shown that the electrode movement of the Reversal forms the anomaly more clearly than that of Normal when the sheeting ore body is situated obliquely, therefore it could be ascertained that the electrode movement have to be performed simultaneously in the manner of Normal and Reversal. It was also exhibited that the aspect of the equiresistivity curve forms symmetrically when an assumed ore body (or linear structure) is situated horizontally or vertically, that is, symmetrically, and moreover that the aspect of the equiresistivity curve forms unsymmetrically when an assumed ore body (or linear structure) is situated obliquely. On the basis of these experimental analysis it is thought that it can be inferred from the aspect of equiresistivity curve whether an assumed ore body is obliquely situated or not.

• The Journal of the Petrological Society of Korea
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• v.25 no.1
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• pp.85-88
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• 2016

The new rocks of the oceanic crust, like basalt, are created in the mid-oceanic ridge, and the magnetic polarities of the rocks are supposed to be oriented as following the Earth's magnetic field. An extensive magnetic survey of total field at sea level reveals mainly unusual north-south magnetic stripes parallel to the axis of the mid-oceanic ridge, especially in the Atlantic Ocean. From this stripes the Earth's magnetic field is considered as repeatedly 'flipped'(the N pole becoming the S pole, and vice versa) and many times over geological time. The discovery of stripes of alternately normal and reversed-magnetized rocks forming the ocean floor has been a key evidence for the sea-floor spreading, continental drift, and plate tectonics. This study introduces a simple apparatus to explain a possible mechanism of the magnetic reversal in the new oceanic crust, which makes a magnetic stripe adjacent to the mid-oceanic ridge. The apparatus shows a bar magnet effect of adjoined stripes to have a special magnetic polarity on the rocks in the center of the mid-oceanic ridge. The new magnetic stripe seems to be generated not only by Earth's magnetic field, but also by neighbored stripes in the mid-oceanic ridge, acting as a bar magnet.

• Journal of Ginseng Research
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• v.25 no.2
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• pp.74-81
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• 2001

The effect of Korean Ginseng saponins (total saponin, PD saponin and PT saponin) on the serotonin type 3 receptor, which is known to be involved in nausea and vomiting following anticancer chemotherapy or the general anesthesia, was investigated. after in vitro transcribed recombinant serotonin type 3 receptor in the Xenopus laevis oocyte, classic two electrodes voltage clamp technique was used. All of ginseng saponins inhibited the response of the agonist, serotonin, on the serotonin type 3 receptor in a dose-dependent manner. PT saponin showed to have the inhibitory effect more than 2 times as potent as PD saponin. Total saponin shifted the serotonin dose response plot to the right (EC$\_$50/, 0.70$\pm$0.17 $\mu$M into 3.57$\pm$1.42 $\mu$M, and Hill coefficient, 2.14$\pm$0.60 into 1.52$\pm$1.00). Ginseng saponin did not change the reversal potential (∼0 mV) of serotonin type 3 receptor. These results suggest that Korean ginseng saponin may have the inhibitory effect on serotonin type 3 receptor.

• Archives of Pharmacal Research
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• v.22 no.2
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• pp.119-123
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• 1999

Parathyroid hormone (PTH) treatment of bone and kidney-derived cells not only activates adenyly cyclase buy also increases intracellular free calcium, and translocates protein kinase C (PKC) from cytosol to plasma membranes. We have found that acute phorbol ester pretreatment significantly decreases PTH-induced calcium transients and the effect of phorbol ester was antagonized by staurosporine (ST). Although the major effect of ST in that study was the reversal of the action of phorbol ester, it appeared that ST may also have promoted the effect of PTH directly. To further investigate the observation, we examined the effect of ST on the intracellular calcium transients induced by PTH and $\alpha$-thrombin ($\alpha$-TH). For calcium transient experiments, UMR-106 cells were loaded with 2 mM fluo-acetoxymethylester for 30 min at room temperature. The cells were then washed and suspended in buffer containing 1 mM calcium. Fluorescence was detected at 530 nm, with excitation at 505 nm. ST alone did not cause calcium transients, but enhanced the transients elicited by PTH response. added 5 min before the hormone. Another protein kinase inhibitor H-7 likewise enhanced the calcium responses elicited by PTH, while genistein did not affect PTH response. Calcium transients elicited by $\alpha$-TH were also enhanced by ST. The results suggest that there might be tonically activated endogenous protein kinase(s) which inhibit calcium signaling of some calcemic agents.

• The Korean Journal of Pain
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• v.12 no.1
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• pp.1-7
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• 1999

Background: To study the role of spinal alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors in pain behaviors caused by mild burn, we examined the effect of intrathecal administered ACEA 2085, which has been recently characterized as a high potency competitive AMPA receptor antagonist, on the thermal hyperalgesia state induced by mild burn. Methods: A thermal injury was induced by applying the left hind paw to a thermal surface ($52.5^{\circ}C$) for 45 sec. Thermal escape latency of the hind paw was determined using an underglass thermal stimulus. Thirty min after thermal injury, the paw withdrawal latency (PWL) in injured paw of all groups fell from 10~12 sec to 5~7 sec. At that time, ACEA 2085 (0.01~0.1 mcg) and 6-cyano-7-nitroquinoxalinedione (CNQX, 1~30 mcg) were injected through intrathecal heters in rats with mild burn injury on the right hindpaw. And then, PWL were measured in the both hindpaw every 30 minutes for about three hours. Results: The intrathecal injection of ACEA 2085 produced a dose dependent reversal of the hyperalgesia in the right hindpaw and more potent than CNQX, but had no effect upon the response latency of the normal left hind paw even at the largest doses. All effects were observed at doses that had no significant effect upon motor function. Conclusions: Intrathecal ACEA 2085, highly selective AMPA receptor antagonist produce a dose- dependent reversal of the thermal hyperalgesia evoked mild burn injury. These results suggested that spinal AMPA receptor play an important role in the hyperalgesia induced by mild burn injury.

• Journal of the Korea Concrete Institute
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• v.28 no.2
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• pp.187-196
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• 2016

The current seismic design code prescribes that coupling beam should be reinforced using diagonally bundled bars. However, the use of a diagonally bundled bars has a negative effect on constructability and economic efficiency. In the present study, the seismic performance of 4 coupling beams with the different details of reinforcement was evaluated through a cyclic reversal loading test. The specimens were constructed to measure the results of the experimental variable regarding the details of shear reinforcement. Next, the seismic performance of the coupled shear wall system evaluated by methods proposed in the FEMA P695. The cyclic reversal loading test results of this study showed that the performance of coupling beams with relaxed reinforcement detail was almost similar to that of a coupling beam with the ACI detail and meet the level which requested from standard. The result of the seismic evaluation showed that all coupling beams are satisfied with the design code and seismic performance.

• Biomolecules & Therapeutics
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• v.3 no.4
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• pp.245-250
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• 1995

The carbon tetrachloride($CCl_4$) has been demonstrated to have a hepatotoxic effect in human or many other species. To investigate the enzyme induction of mixed function oxygenases in liver of male Sprague-Dawley rats a single 0.1, 0.5 mι/kg dose of carbon tetrachloride were given. At 24 hr after a single dose of 0.1 mι CC1$_4$/kg weight, methanol extract of Hedera rhombea leaves was administered with 100, 500 mg/kg weight. Assays of 7-ethoxyresorufin-Ο-deethylation(EROD),7-benzyloxyresorufin-Ο-deathylation(BROD),4-nitro-phenol-UDP-glucuronosyltransferase(UDPGT), Western blot and RNA slot blot were used as representatives of the activities of cytochrome P-450 enzymes. The change of the activity of CYP1A1 form measured by EROD assay and Western analysis using 1-7-1 monoclonal antibody was not observed. The activity CYP2B1 form by BROD assay and using 2-66-3 monoclonal antibody was remarkably increased. Elevated level of CYP2B1 mRNA was shown by slot hybridization with 2B1-specific probe. Administration of methanol extract of Hedera rhombea leaves reversed the enzyme activity and the level of mRNA, which suggest the chemoprotective effect of methanol extracts of Hedera rhombea leaves to carbon tetrachloride hepatotoxlcity.