• Molecules and Cells
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• 제22권2호
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• pp.233-238
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• 2006

Endogenous salicylic acid (SA) and its predominant conjugates, SA 2-O-${\beta}$-D-glucoside (SAG) and the glucose ester of SA (SGE), increase dramatically during plant defense responses. Here I report the isolation and characterization of an Arabidopsis thaliana UDP-glucose:SA glucosyltransferase1 (AtSGT1) gene using a tobacco SGT gene previously reported, whose product catalyzes the formation of both SAG and SGE. The recombinant AtSGT1 protein had significant activities with SA and benzoic acid, and synthesized SAG and SGE. Northern blot analysis showed that AtSGT1 was rapidly induced both by exogenous SA and infection with the bacterial pathogen Pseudomonas syringae, indicating that pathogen-inducible AtSGT1 expression is an early disease response and may be involved in the accumulation of glucosyl SA during pathogenesis.

• 한국광학회:학술대회논문집
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• 한국광학회 2001년도 제12회 정기총회 및 01년도 동계학술발표회
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• pp.4-5
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• 2001

Advent of lasers offering high intensity beam has opened the glass to the nonlinear optic (NLO). The high electric field associated with such laser beams can be so large that high order components of the glass polarization can be measured. Such development is of scientific and technological interests in particular in systems involving an intensity-dependent refractive index and/or ultra-fast response (＜10$\^$-12/s). From a scientific viewpoint the NLO response intensity must be understood as a function of the glass composition. On the other hand, large family of applications are presently under investigation in various fields of optical materials or systems e.g. laser glasses for fusion energy, soliton propagation for ultra-long distances, ultra-fast-switching, optical storage etc....(omitted)

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2013년도 춘계학술대회 논문집
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• pp.38-38
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• 2013

The mechanical cues that adherent cells derive from the extracellular matrix (ECM) can effect dramatic changes in cell migration, proliferation, and differentiation. Using a thin film of Type I collagen fibrils comprised of 100 nm to 200 nm collagen fibrils overlaying a bed of smaller fibrils, changes in cellular response to systematically controlled changes in mechanical properties of collagen was investigated. Further, an experimental and modeling approaches to calculate the elastic modulus of individual collagen fibrils, and thereby the effective stiffness of the entire collagen thin film matrix, from atomic force microscopy force spectroscopy data was performed. These results demonstrate an approach to analysis of fundamental properties of thin, heterogeneous, organic films, and add further insights into the mechanical properties of collagen fibrils that are of relevance to cell response to the ECM.

• Earthquakes and Structures
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• 제3권5호
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• pp.629-647
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• 2012

Multiple acceleration sequences of earthquake ground motions have been observed in many regions of the world. Such ground motions can cause large damage to the structures due to accumulation of inelastic deformation from the repeated sequences. The dynamic analysis of inelastic structures under repeated acceleration sequences generated from simulated and recorded accelerograms without sequences has been recently studied. However, the characteristics of recorded earthquake ground motions of multiple sequences have not been studied yet. This paper investigates the gross characteristics of earthquake records of multiple sequences from an engineering perspective. The definition of the effective number of acceleration sequences of the ground shaking is introduced. The implication of the acceleration sequences on the structural response and damage of inelastic structures is also studied. A set of sixty accelerograms is used to demonstrate the general properties of repeated acceleration sequences and to investigate the associated structural inelastic response.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권6호
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• pp.516-521
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• 2006

The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens IuxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

• Computers and Concrete
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• 제7권2호
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• pp.169-190
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• 2010

The focus of this research effort was characterization of the flexural and tensile properties of a specific ultra-high-strength, fiber-reinforced concrete material. The material exhibited a mean unconfined compressive strength of approximately 140 MPa and was reinforced with short, randomly distributed alkali resistant glass fibers. As a part of the study, coupled experimental, analytical and numerical investigations were performed. Flexural and direct tension tests were first conducted to experimentally characterize material behavior. Following experimentation, a micromechanically-based analytical model was utilized to calculate the material's tensile failure response, which was compared to the experimental results. Lastly, to investigate the relationship between the tensile failure and flexural response, a numerical analysis of the flexural experiments was performed utilizing the experimentally developed tensile failure function. Results of the experimental, analytical and numerical investigations are presented herein.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.29-29
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• 2003

Incompatible plant-pathogen interactions result in the rapid cell death response known as hypersensitive response (HR) and activation of host defense-related genes. To understand the molecular and cellular mechanism controlling defense response better, several approaches including isolation and characterization of novel genes, promoter analysis of those genes, protein-protein interaction analysis and reverse genetic approach etc. By using the yeast two-hybrid system a clone named Tsipl, Tsil -interacting protein 1, was isolated whose translation product apparently interacted with Tsil, an EREBP/AP2 type DNA binding protein. RNA gel blot analysis showed that the expression of Tsipl was increased by treatment with NaCl, ethylene, salicylic acid, or gibberellic acid. Transient expression analysis using a Tsipl::smGFP fusion gene in Arabidopsis protoplasts indicated that the Tsipl protein was targeted to the outer surface of chloroplasts. The targeted Tsipl::smGFP proteins were diffused to the cytoplasm of protoplasts in the presence of salicylic acid (SA) The PEG-mediated co-transfection analysis showed that Tsipl could interact with Tsil in the nucleus. These results suggest that Tsipl-Tsil interaction might serve to regulate defense-related gene expression. Basically the useful promoters are valuable tools for effective control of gene expression related to various developmental and environmental condition.(중략)

• BMB Reports
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• 제28권2호
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• pp.155-161
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• 1995

A nicotinic acetylcholine receptor (nAchR) isolated from the electric tissues of Torpedo californica has been reconstituted into a vesicle comprising a bifunctional azo-ligand (Bae 1) compound, and a liposome containing phospholipids and cholesterol (1 : 1, w/w). The liposome-mediated reconstituted receptor showed a concentration-dependent response to cholinergic drugs in a lithium ion flux assay. This liposome-mediated reconstituted nAchR was immobilized onto an electrode using various synthetic polymers which were tested for their response to the cholinergic ligands. The immobilized nAchR not only exhibited a linear response to a wide range of cholinergic ligand concentrations but also retained an operational stability which lasted for longer than 6 days. Thus, this result provides a basis for application of the immobilized nAchR-based biosensor in detecting cholinergic ligands in vitro.

• 전기학회논문지
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• 제56권5호
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• pp.915-920
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• 2007

AIN-based film bulk acoustic resonator (FBAR) devices which adopt a membrane-type configuration such as Mo/AIN/bottom-metal/Si are fabricated by employing a novel process. The proposed resonator structure does not require any supporting layer above the substrate, which leads to the reduction in energy loss of the resonators. For all the FBAR devices, the frequency response characteristics are measured and the device parameters, such as return loss and input impedance, are extracted from the frequency responses, and analyzed in terms of the various metals such as Al. Cu, Mo, W used in the bottom-electrode. The mass-loading effect caused by the used bottom-electrode metals is found to be the main reason for the difference revealed in the measured characteristics of the fabricated FBAH devices. The results obtained in this study also show that the degree of match in lattice constant and thermal expansion coefficient hetween piezoelectric layers and electrode metals is crucial to determine the device performance of FEAR.

• Structural Engineering and Mechanics
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• 제68권4호
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• pp.423-442
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• 2018

In this paper, masonry infilled reinforced concrete (RC) frames are analyzed through a probabilistic approach. A macro-modeling technique, based on an equivalent diagonal pin-jointed strut, has been resorted to for modelling the stiffening contribution of the masonry panels. Since it is quite difficult to decide which mechanical characteristics to assume for the diagonal struts in such simplified model, the strut width is here considered as a random variable, whose stochastic characterization stems from a wide set of empirical expressions proposed in the literature. The stochastic analysis of the masonry infilled RC frame is conducted via the Probabilistic Transformation Method by employing a set of space transformation laws of random vectors to determine the probability density function (PDF) of the system response in a direct manner. The knowledge of the PDF of a set of response indicators, including displacements, bending moments, shear forces, interstory drifts, opens an interesting discussion about the influence of the uncertainty of the masonry infills and the resulting implications in a design process.