• Title/Summary/Keyword: Respirometer

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Measurement of Biological Activity in Pilot Scale Dyeing Wastewater Process by Using Respirometer (호흡률 측정기를 이용한 파일럿 스케일 염색폐수처리 장치에서의 생물학적 활성 측정)

  • Jeon Hyun Hee;Choi Kwang Keun;Yoon In Jun;Lee Jin Won
    • KSBB Journal
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    • v.19 no.5
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    • pp.390-393
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    • 2004
  • Oxygen uptake rate (OUR) was used as an indicator of microbial activity. In this study OUR at dyeing wastewater in the pilot plant was monitored to examine biological activity. Correlation between inlet COD concentration and maximum OUR showed that maximum OUR was proportional to inlet COD concentration. Changes in the OUR values reflected the changing waste load in the reactor. Consequently, OUR can be used to estimate biological activity of inlet COD concentration. This study showed that biodegradable COD at dyeing wastewater could be calculated from OUR and yield coefficient. Non-biodegradable COD was able to be calculated from a difference between initial COD concentration and biodegradable COD.

Effects of Sodium Cyanide (NaCN) on the Endogenous Rhythm of the Oxygen Consumption Rate in the Black Rockfish Sebastes schlegeli

  • Kim, Wan-Soo;Kim, Jong-Wook;Lee, Jae-Hak;Huh, Sung-Hoe
    • Ocean Science Journal
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    • v.43 no.2
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    • pp.107-113
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    • 2008
  • Laboratory bioassays were conducted to test the acute toxicity effects of sudden exposure to sodium cyanide (NaCN) on the endogenous rhythm of the oxygen consumption rate (OCR) in the black rockfish Sebastes schlegeli. The OCR of the black rockfish(n = 14, total length = $20.4{\pm}1.16\;cm$, wet weight = $158{\pm}25\;g$) was measured with an automatic intermittent-flow-respirometer. OCR decreased significantly when experimental fish were exposed to NaCN. When exposed to 10 ppb NaCN, fish were able to recover their OCR rhythmic activities. When fish were exposed to 20 ppb, however, the metabolic activity rhythms were not recovered. These results suggest that exposure to NaCN concentrations over 20 ppb cause severe physiological damage to the endogenous rhythms of black rockfish.

The Effect of Nonionic Surfactants on the Solubility and Biodegradation of PAHs in Soil Slurry (PAHs의 용해도와 생분해에 미치는 비이온계 계면활성제의 영향)

  • 박종섭;김인수
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1998.11a
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    • pp.174-177
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    • 1998
  • The effects of surfactants affecting polycyclic Aromatic Hydrocarbon(PAHs) solubility and biodegradation in soil slurry were investigated. The critical micelle concentration(CMC) values of surfactants used in this study were 12.7mg/L(Brij 30), 13.4mg/L(Tween 80), 13.6mg/t(Triton X-100). The solubility of PAH increased as the Hydrophile-Lipophile Balance(HLB) value of surfactant decrease. At surfactant biodegradation and toxicity experiement using respirometer, Brij 30 did not show any toxic effect and substrate inhibition upon the level of 1.5g/L. Also, biodegradation of Brij 30 gave no reduction on the phenanthrene biodegradation rate. When the desorption rate of phenanthrene between sand and clay is compared, lower percentage of phenanthrene was desorbed at clay because of the larger surface aera and higher organic content of clay. At the biodegradation experiments of phenanthrene in soil slurry phase, more than 90% of initial phenanthrene adsorbed onto both sand and clay were biodegraded by phenanthrene- acclimated cultures.

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Effects of Some Carbohydrates and Ammonium Sulfate on Lignin Degradation by Pseudomonas diminuta (탄수화물과 황산암모늄이 Pseudomonas diminuta의 리그닌 분해에 미치는 영향)

  • 김규중;신광수;맹진수;성치남
    • Korean Journal of Microbiology
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    • v.26 no.2
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    • pp.129-136
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    • 1988
  • To investigate the influence of cosubstrate supplement and ammonium sulfate on lignin degradation by Pseudomonas diminuta KM-4-2, isolated in the laboratory, the strain was cultured on the lignin media which contained lignin as a source of carbon and the culture filtrate was analyzed by Sephadex G-75 column chromatography. It was found that polymerization was not appeared unlike wood-rot fungi. When the carbohydrates were added, the peak of lignin at 280nm by UV scanning spectra of the filtrate, was significantly increased. In order to determine the effect of ammonium sulfate on the ligninolytic activity, the isolated strain was incubated in the media containing 0.1%, 0.25% and 0.5% of nitrogen concentration in the Warburg flask and the rate of oxygen uptake was esitmated by Warbuge Respirometer. As a result, the activity was maximum at 0.1% of nitrogen concentration and thereafter decreased in parallel with nitrogen concentration.

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Swimming Performance Evaluation of Four Freshwater Fish Species from the South Korea (국내에 서식하는 담수어류 4종에 대한 유영능력 평가)

  • Misheel, Bold;Kim, Kyu-Jin;Min, Kun-Woo;Jang, Min-Ho
    • Korean Journal of Ecology and Environment
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    • v.52 no.2
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    • pp.118-125
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    • 2019
  • Swimming performance of fish is an important factor in the survival of fish. Also, swimming performance of fish is used in the form of habitat, or as a condition to consider when creating a fish ladder. However in Korea, researches in swimming performance of Korean freshwater fish were scarce and inadequate in some part, thus fish ladders were installed without considering their swimming performance. Therefore, in this study, we measured swimming performance of 4 Korean freshwater fish species to consider importance of swimming performance test. The fish used in this study were Carassius auratus, Zacco koreanus, Gnathopogon strigatus, Acheilognathus lanceolata intermedia species which was collected during October to November, 2018 at Geum River, and measurement for swimming speed of each fish was done by using $Loligo^{(R)}$ System, swim tunnel respirometer in January to February of 2019. The average value of the burst critical swimming speed ($U_{crit}$) for each species was $0.8{\pm}0.04m\;s^{-1}$ for C. auratus, $0.77{\pm}0.04m\;s^{-1}$ for Z. koreanus, $0.95{\pm}0.04m\;s^{-1}$ for G. strigatus, $0.73{\pm}0.03m\;s^{-1}$ for A. lanceolata intermedia and the average value of prolonged critical swimming speed was $0.54m\;s^{-1}$ for C. auratus, $0.67m\;s^{-1}$ for Z. koreanus, $0.7m\;s^{-1}$ for G. strigatus, $0.54m\;s^{-1}$ for A. lanceolata intermedia. Since the fish used in this experiment were collected from a small part of the water system in Korea and there were only 4 species, they were not enough to represent the species that inhabit the entire Korean water system. It will be necessary to continue evaluating the swimming performance of other freshwater species in Korea.

Effects of Soil-Amended Bottom Ash on Decomposition Rates of Organic Matter as Investigated by an Enforced-Aeration Respirometer (호기순환 호흡계를 이용한 토양처리 석탄바닥재의 유기물 분해에 미치는 영향)

  • Jung, Seok-Ho;Chung, Doug-Young;Han, Gwang-Hyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.2
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    • pp.253-259
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    • 2012
  • Disposal of high amount of coal combustion by-products, such as fly ash and bottom ash, is of a great concern to the country, due to the huge treatment cost and land requirement. On the other hand, those coal-ash wastes are considered to have desirable characteristics that may improve physical, chemical, and biological properties of soils. Especially, compared with fly ash, bottom ash has a larger particle size, porous surface area, and usable amount of micronutrients. In the present study, we examined bottom as a soil amendment for mitigating $CO_2$ emission and enhancing carbon sequestration in soils fertilized with organic matter (hairy vetch, green barely, and oil cake fertilizer). Through laboratory incubation, $CO_2$ released from the soil was quantitatively and periodically monitored with an enforced-aeration and high-temperature respirometer. We observed that amendment of bottom ash led to a marked reduction in $CO_2$ emission rate and cumulative amount of $CO_2$ released, which was generally proportional to the amount of bottom ash applied. We also found that the temporal patterns of $CO_2$ emission and C sequestration effects were partially dependent on the relative of proportion labile carbon and C/N ratio of the organic matter. Our results strongly suggest that amendment of bottom ash has potential benefits for fixing labile carbon as more stable soil organic matter, unless the bottom ash contains toxic levels of heavy metals or other contaminants.

Respirometry for the Assessment of Organics Biodegradability in Municipal Wastewater: I. Respirometry (호흡률법에 의한 하수의 생분해 특성 평가: I. 호흡률법)

  • Kim, Dong Han;Kim, Hee Jun;Chung, Tai Hak
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.1
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    • pp.29-36
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    • 2004
  • Organics may be divided into biodegradable and nonbiodegradable fractions on the basis of biodegradability. Biodegradable organics may be subdivided into readily and slowly biodegradable fractions. As this biodegradability of organics in municipal wastewater has a great influence on the efficiency of a biological nutrient removal process, it has been assessed by respirometry. The respirometer, which consisted of a respiration chamber and a respiration cell, was used to measure the respiration rate of biomass utilizing the readily biodegradable organics. The readily biodegradable organics are about 10% of the COD in municipal wastewater. The adequate ratio of wastewater to sludge volume and the concentration of sludge are required in measuring the respiration rate due to the readily biodegradable organics. By using a biochemical oxygen demand test, the slowly biodegradable organics including biomass are estimated about 66% of COD. The soluble inert organics are about 11% of COD. On the basis of mass balance, the particulate inert organics are estimated about 13% of COD.

Critical Thermal Maximum (CTM) of Cultured Black Rockfish, Sebastes schlegeli

  • Kim Wan-Soo;Yoon Seong-Jin;Gil Joon-Woo
    • Fisheries and Aquatic Sciences
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    • v.6 no.2
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    • pp.59-65
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    • 2003
  • The critical thermal maximum (CTM) of black rockfish, Sebastes schlegeli, was evaluated. Black rockfish were acclimated at $24^{\circ}C$, and then exposed to temperatures from 24 to $33^{\circ}C$. Black rockfish were kept in constant darkness and subjected to a gradual temperature increase $(1 ^{\circ}C\;12^{-1})$. The oxygen consumption rate (OCR) was measured using an automatic intermittent­flow-respirometer (AIFR) during the exposure period (from 119.3 to 143.5 h). The OCR increased from 94.5 to 214.2mL $O_2 kg^{-1}\;ww\;h^{-1}$ as the temperature rose from 24 to $29.4-30.9^{\circ}C$. Subsequently, the OCR increased abruptly, reaching 245.8-412.7mL $O_2 kg^{-1}\;ww\;h^{-1}$ at $32^{\circ}C$. This study suggests that the CTM for black rockfish is $29.4-30.9^{\circ}C$ when temperature is increased at $1^{\circ}C\;12h^{-1}$ following acclimation at $24^{\circ}C$.

Rate of Oxygen Uptake in Antheraea mylitta in Various Stages of Development and during Diapause

  • Rath S. S.;Negi B. B. S.;Singh B. M. K.;Thangavelu K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.1
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    • pp.45-49
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    • 2005
  • Respiration in Antheraea mylitta was studied using constant pressure respirometer. Oxygen uptake in different stages of life showed that moth has highest rate of oxygen uptake as compared to larva and pupa. At each stage significant sexual differentiation was observed. Depression in the rate of oxygen uptake in diapausing pupa was found to be higher in male ($65.75\%$) than female ($60.65\%$) as compared to non-diapausing counterparts. During diapause lowest oxygen uptake was recorded in the month of February, and were in the order of February < January < December < March < November < April < May < June in male, and, February < January < December < March < April < November < May < June in female. Significant sexual differentiation in oxygen uptake was recorded throughout the period of diapause (November to June) where female pupae registered lower rate of oxygen uptake than that of male. Oxygen uptake in female pupae declined upto $28.89\%$ in non-diapausing and $18.29\%$ in diapausing broods over male. Highest respiratory quotient value was recorded in the moth of November (0.68 in male and 0.70 in female) and, the lowest in the month of March in male (0.54) and May in female (0.55). Percentage loss in pupa weight always remained at a significantly higher level in male (except in February and March). The lowest oxygen uptake rate and weight loss in the month of February shows that the pupae were at peak of their dormancy during this month.

A Comparative Study on COD Fractionation Methods of Wastewater (하수의 COD 분류 시험 방법에 관한 비교 연구)

  • Kim, Sung-Hong;Yun, Jung-Won;Choi, Young-Gyun
    • Journal of Korean Society of Water and Wastewater
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    • v.24 no.4
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    • pp.387-394
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    • 2010
  • The influent COD of municipal wastewater has been divided into 4 fractions; readily soluble biodegradable, slowly particulate biodegradable, soluble and particulate unbiodegradable COD. The mathematical modeling of biological wastewater treatment processes and the design and operation of nutrient removal plants require a reliable and accurate estimate of the composition of influent wastewater COD. COD utilization rate is proportional to the oxygen uptake rate(OUR), so a batch biodegradation test with OUR measurement has been effectively used for the determination of COD fractionation. But the mathematical model of COD utilization and heterotrophs synthesis is essential to interpret the OUR measurement. Mamais method is another method for determining readily biodegradable soluble COD. Like the OUR test method, batch biodegradation test is necessary but it does not require mathematical model. These two methods for determining COD fractionation are introduced here in detail. Experimental results showed that COD composition by Mamais method is not different to that by OUR test method so, either of them can be used.