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Locating QTLs controlling overwintering seedling rate in perennial glutinous rice 89-1 (Oryza sativa L.)

  • Deng, Xiaoshu;Gan, Lu;Liu, Yan;Luo, Ancai;Jin, Liang;Chen, Jiao;Tang, Ruyu;Lei, Lixia;Tang, Jianghong;Zhang, Jiani;Zhao, Zhengwu
    • Genes and Genomics
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    • v.40 no.12
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    • pp.1351-1361
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    • 2018
  • A new cold tolerant germplasm resource named glutinous rice 89-1 (Gr89-1, Oryza sativa L.) can overwinter using axillary buds, with these buds being ratooned the following year. The overwintering seedling rate (OSR) is an important factor for evaluating cold tolerance. Many quantitative trait loci (QTLs) controlling cold tolerance at different growth stages in rice have been identified, with some of these QTLs being successfully cloned. However, no QTLs conferring to the OSR trait have been located in the perennial O. sativa L. To identify QTLs associated with OSR and to evaluate cold tolerance. 286 $F_{12}$ recombinant inbred lines (RILs) derived from a cross between the cold tolerant variety Gr89-1 and cold sensitive variety Shuhui527 (SH527) were used. A total of 198 polymorphic simple sequence repeat (SSR) markers that were distributed uniformly on 12 chromosomes were used to construct the linkage map. The gene ontology (GO) annotation of the major QTL was performed through the rice genome annotation project system. Three main-effect QTLs (qOSR2, qOSR3, and qOSR8) were detected and mapped on chromosomes 2, 3, and 8, respectively. These QTLs were located in the interval of RM14208 (35,160,202 base pairs (bp))-RM208 (35,520,147 bp), RM218 (8,375,236 bp)-RM232 (9,755,778 bp), and RM5891 (24,626,930 bp)-RM23608 (25,355,519 bp), and explained 19.6%, 9.3%, and 11.8% of the phenotypic variations, respectively. The qOSR2 QTL displayed the largest effect, with a logarithm of odds score (LOD) of 5.5. A total of 47 candidate genes on the qOSR2 locus were associated with 219 GO terms. Among these candidate genes, 11 were related to cell membrane, 7 were associated with cold stress, and 3 were involved in response to stress and biotic stimulus. OsPIP1;3 was the only one candidate gene related to stress, biotic stimulus, cold stress, and encoding a cell membrane protein. After QTL mapping, a total of three main-effect QTLs-qOSR2, qOSR3, and qOSR8-were detected on chromosomes 2, 3, and 8, respectively. Among these, qOSR2 explained the highest phenotypic variance. All the QTLs elite traits come from the cold resistance parent Gr89-1. OsPIP1;3 might be a candidate gene of qOSR2.

Development of Standard Method for Quality Innovation to Strengthen Global Competitiveness and Create Management Performance of Small and Medium-sized Manufacturing Firms (중소 제조기업의 글로벌 품질경쟁력 강화 및 경영성과 창출을 위한 품질혁신 표준방법론 개발)

  • Park, Jong Kab;Kim, Youn Sung
    • Journal of Korean Society for Quality Management
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    • v.46 no.4
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    • pp.843-862
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    • 2018
  • Purpose: The purpose of this study was to develop quality innovation techniques specialized for the small and medium-sized businesses. which account for the majority of Korean companies, were having a hard time utilizing the widely recognized quality innovation techniques due to resource constraints. Methods: First, we do review the existing Single PPM and 6 Sigma. And investigate the utilization of these methods including Toyota Production System. Second, we devised a four-step problem-solving methodology based on recent trends in quality innovation such as Simple, Speedy, and Smart. Third, we do survey on frequently used tools for quality innovation. Many opinion leaders including quality consultants and professors answered and gave us valuable comments about our selected quality tools. Finally, we do specify and map tools to each step of PASS. Results: In 2017, 167 companies participated in the quality innovation support business for small businesses according to the Korea Chamber of Commerce & Industry. We conducted performance checks on 167 companies that had completed the "PASS" projects. For the purpose of evaluating improvement performance, the survey was carried out using a structured questionnaire during the field visit of these companies mentioned above. For the reference, 165 out of 167 companies (98.8 % response rate) responded to the questionnaire and conducted performance analysis based on it. According to the survey, 97.6 percent of the respondents were very satisfied with their overall satisfaction with the quality innovation support projects for small and medium sized enterprises in 2017. Also, 93.3 % of the respondents were satisfied with the results of level of the target achievement. As a result, 160 companies (97.0 % of the participating companies) hope to partic ipate in the quality improvement project using "PASS" once again. Conclusion: In this paper, we introduce the new quality innovation methodology, which is named as 'PASS', It could support the long-range business plan of the small and medium-sized businesses to achieve total customer satisfaction resulting in increased market share and improved profit margin. The most small companies can use this "PASS" technique more easily, quickly and most efficiently than their existing known quality innovation techniques such as Six Sigma and Single PPM, etc.

A Study on the View Characteristics of the Pavilion around Gyeongpo Lake (경포호 누정의 조망특성에 관한 연구)

  • Han, Gab-Soo
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.39 no.4
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    • pp.66-76
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    • 2021
  • The purpose of this study was to analyze the transition process of Gyeongpo lake and to analyze the viewing characteristics of each period in the pavilion. The view characteristics at pavilion were analyzed by overlapping classification maps of landscape types by period with the view range map from pavilion. The circumference and area of Gyeongpo lake have been reduced to 15.2km and 3.9km2 in the 1750s, 9.0km and 1.7km2 in the 1933, and now 4.41km and 1.0km2. By period, the largest decrease in the proportion of Gyeongpo lake viewed from Pavilion was Hwanseonjeong, which decreased by 48.0% from the 1750 to 2017. Hwallaejeong, Haeunjeong, and Hohaejeong were found to be impossible to view Gyeongpo lake as some of the adjacent lakes were changed to agricultural land. Pavilion has great significance as a space for traditional culture created in the background of humanities and natural environments. For the succession and development of Pavilion culture, it is necessary to use it as a cultural resource, to continuously manage the building, and to partially restore Gyeongpo lake considering the function of each pavilion.

Analysis of Time Series Changes in the Surrounding Environment of Rural Local Resources Using Aerial Photography and UAV - Focousing on Gyeolseong-myeon, Hongseong-gun - (항공사진과 UAV를 이용한 농촌지역자원 주변환경의 시계열 변화 분석 - 충청남도 홍성군 결성면을 중심으로 -)

  • An, Phil-Gyun;Eom, Seong-Jun;Kim, Yong-Gyun;Cho, Han-Sol;Kim, Sang-Bum
    • Journal of Korean Society of Rural Planning
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    • v.27 no.4
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    • pp.55-70
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    • 2021
  • In this study, in the field of remote sensing, where the scope of application is rapidly expanding to fields such as land monitoring, disaster prediction, facility safety inspection, and maintenance of cultural properties, monitoring of rural space and surrounding environment using UAV is utilized. It was carried out to verify the possibility, and the following main results were derived. First, the aerial image taken with an unmanned aerial vehicle had a much higher image size and spatial resolution than the aerial image provided by the National Geographic Information Service. It was suitable for analysis due to its high accuracy. Second, the more the number of photographed photos and the more complex the terrain features, the more the point cloud included in the aerial image taken with the UAV was extracted. As the amount of point cloud increases, accurate 3D mapping is possible, For accurate 3D mapping, it is judged that a point cloud acquisition method for difficult-to-photograph parts in the air is required. Third, 3D mapping technology using point cloud is effective for monitoring rural space and rural resources because it enables observation and comparison of parts that cannot be read from general aerial images. Fourth, the digital elevation model(DEM) produced with aerial image taken with an UAV can visually express the altitude and shape of the topography of the study site, so it can be used as data to predict the effects of topographical changes due to changes in rural space. Therefore, it is possible to utilize various results using the data included in the aerial image taken by the UAV. In this study, the superiority of images acquired by UAV was verified by comparison with existing images, and the effect of 3D mapping on rural space monitoring was visually analyzed. If various types of spatial data such as GIS analysis and topographic map production are collected and utilized using data that can be acquired by unmanned aerial vehicles, it is expected to be used as basic data for rural planning to maintain and preserve the rural environment.

Sensitivity Analysis of Wake Diffusion Patterns in Mountainous Wind Farms according to Wake Model Characteristics on Computational Fluid Dynamics (전산유체역학 후류모델 특성에 따른 산악지형 풍력발전단지 후류확산 형태 민감도 분석)

  • Kim, Seong-Gyun;Ryu, Geon Hwa;Kim, Young-Gon;Moon, Chae-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.17 no.2
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    • pp.265-278
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    • 2022
  • The global energy paradigm is rapidly changing by centering on carbon neutrality, and wind energy is positioning itself as a leader in renewable energy-based power sources. The success of onshore and offshore wind energy projects focuses on securing the economic feasibility of the project, which depends on securing high-quality wind resources and optimal arrangement of wind turbines. In the process of constructing the wind farm, the optimal arrangement method of wind turbines considering the main wind direction is important, and this is related to minimizing the wake effect caused by the fluid passing through the structure located on the windward side. The accuracy of the predictability of the wake effect is determined by the wake model and modeling technique that can properly simulate it. Therefore, in this paper, using WindSim, a commercial CFD model, the wake diffusion pattern is analyzed through the sensitivity study of each wake model of the proposed onshore wind farm located in the mountainous complex terrain in South Korea, and it is intended to be used as basic research data for wind energy projects in complex terrain in the future.

Research on Bridge Maintenance Methods Using BIM Model and Augmented Reality (BIM 모델과 증강현실을 활용한 교량 유지관리방안 연구)

  • Choi, Woonggyu;Pa Pa Win Aung;Sanyukta Arvikar;Cha, Gichun;Park, Seunghee
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.44 no.1
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    • pp.1-9
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    • 2024
  • Bridges, which are construction structures, have increased from 584 to 38,405 since the 1970s. However, as the number of bridges increases, the number of bridges with a service life of more than 30 years increases to 21,737 (71%) by 2030, resulting in fatal accidents due to basic human resource maintenance of facilities. Accordingly, the importance of bridge safety inspection and maintenance measures is increasing, and the need for decision-making support for supervisors who manage multiple bridges is also required. Currently, the safety inspection and maintenance method of bridges is to write down damage, condition, location, and specifications on the exterior survey map by hand or to record them by taking pictures with a camera. However, errors in notation of damage or defects or mistakes by supervisors are possible, typos, etc. may reduce the reliability of the overall safety inspection and diagnosis. To improve this, this study visualizes damage data recorded in the BIM model in an AR environment and proposes a maintenance plan for bridges with a small number of people through maintenance decision-making support for supervisors.

Habitat Distribution Change Prediction of Asiatic Black Bears (Ursus thibetanus) Using Maxent Modeling Approach (Maxent 모델을 이용한 반달가슴곰의 서식지 분포변화 예측)

  • Kim, Tae-Geun;Yang, DooHa;Cho, YoungHo;Song, Kyo-Hong;Oh, Jang-Geun
    • Korean Journal of Ecology and Environment
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    • v.49 no.3
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    • pp.197-207
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    • 2016
  • This study aims at providing basic data to objectively evaluate the areas suitable for reintroduction of the species of Asiatic black bear (Ursus thibetanus) in order to effectively preserve the Asiatic black bears in the Korean protection areas including national parks, and for the species restoration success. To this end, this study predicted the potential habitats in East Asia, Southeast Asia and India, where there are the records of Asiatic black bears' appearances using the Maxent model and environmental variables related with climate, topography, road and land use. In addition, this study evaluated the effects of the relevant climate and environmental variables. This study also analyzed inhabitation range area suitable for Asiatic black and geographic change according to future climate change. As for the judgment accuracy of the Maxent model widely utilized for habitat distribution research of wildlife for preservation, AUC value was calculated as 0.893 (sd=0.121). This was useful in predicting Asiatic black bears' potential habitat and evaluate the habitat change characteristics according to future climate change. Compare to the distribution map of Asiatic black bears evaluated by IUCN, Habitat suitability by the Maxent model were regionally diverse in extant areas and low in the extinct areas from IUCN map. This can be the result reflecting the regional difference in the environmental conditions where Asiatic black bears inhabit. As for the environment affecting the potential habitat distribution of Asiatic black bears, inhabitation rate was the highest, according to land coverage type, compared to climate, topography and artificial factors like distance from road. Especially, the area of deciduous broadleaf forest was predicted to be preferred, in comparison with other land coverage types. Annual mean precipitation and the precipitation during the driest period were projected to affect more than temperature's annual range, and the inhabitation possibility was higher, as distance was farther from road. The reason is that Asiatic black bears are conjectured to prefer more stable area without human's intervention, as well as prey resource. The inhabitation range was predicted to be expanded gradually to the southern part of India, China's southeast coast and adjacent inland area, and Vietnam, Laos and Malaysia in the eastern coastal areas of Southeast Asia. The following areas are forecast to be the core areas, where Asiatic black bears can inhabit in the Asian region: Jeonnam, Jeonbuk and Gangwon areas in South Korea, Kyushu, Chugoku, Shikoku, Chubu, Kanto and Tohoku's border area in Japan, and Jiangxi, Zhejiang and Fujian border area in China. This study is expected to be used as basic data for the preservation and efficient management of Asiatic black bear's habitat, artificially introduced individual bear's release area selection, and the management of collision zones with humans.

A Case Study on the Willow Tree Fence(樹柵) in Gasan(假山) of Cheonggyecheon, Hanyang in the Joseon Dynasty Period (조선시대 한양 청계천 가산(假山)의 버드나무 수책(樹柵)에 관한 연구)

  • SHIM Sunhui;KIM Choongsik
    • Korean Journal of Heritage: History & Science
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    • v.57 no.2
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    • pp.118-141
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    • 2024
  • This study investigates and analyzes ancient literature records and iconographic materials to examine the Willow Tree Fence(樹柵) built on Gasan(假山) Cheonggyecheon(淸溪川) within the Hanyangdoseong, which was deliberately created to prevent flood damage during the Joseon Dynasty. Although there have been research cases related to the willow tree, it is difficult to find research conducted with the purpose of identifying its archetypal value by investigating and analyzing specific use cases of the willow tree and its historical background. Accordingly, this study aims to identify examples of the Willow Tree Fence(樹柵) created in Cheonggyecheon(淸溪川) during the Joseon Dynasty and reinterpret their value by illuminating the background of construction and regional characteristics. The main contents of this study are as follows. It is presumed that floods during the Joseon Dynasty were a great hazard. Between the 16th and 18th centuries, Joseon suffered severe damage from floods. By the time of King Yeongjo, all Four Mountains(四山) of the capital had become bare mountains, which was the cause of frequent floods. In the year of Gyeongjin(庚辰, the 26th year of King Yeongjo's reign, 1760), King Yeongjo dredged the channel bottom of Cheonggyecheon(淸溪川), which overflowed every rainy season, with the Juncheon Project(Channel-Dredging, 濬川事業) and planted willow trees on the mountain on both sides of the Ogan Water Gate(五間水門), as measures to prevent flood damage and soil loss. was implemented. In the <Doseongdo(都城圖)> in 《 Gwangyeodo(廣輿圖)》 produced in the mid-18th century during the reign of King Yeongjo, Gasan(假山), built in front of the Ogan Water Gate(五間水門) is visible, and in the record 『Sinjeung Donggukyeoji Seungnam(新增東國輿地勝)』 In the record, it appears that willows were planted on both sides of the mountain in the year of Gyeongjin(1760). With <Hanyangdoseong Map(漢陽都城圖)> produced in the 46th year of King Yeongjo's reign(1770), it is confirmed that willow trees formed a thick forest on Gasan Mountain near the Ogan Water Gate(五間水門) in the late 18th century. In addition, the Juncheon Project(Channel-Dredging, 濬川事業) and the creation of the Willow Tree Fence(樹柵) continued from the 15th century, the early Joseon Dynasty(朝鮮前期), to the end of the 19th century, the late Joseon Dynasty(朝鮮後期), through the records of ancient literature such as 『Annals of the Joseon Dynasty(朝鮮王朝實錄)』, 『Seungjeongwon Diary(承政院日記)』, and 『Records of Daily Reflections(日省錄)』. This study is meaningful in informing that the willow tree was a unique cultural heritage and traditional landscape resource by investigating the composition and use of the Willow Tree Fence in the Joseon Dynasty, which was a great basis for preventing floods and flood damage, as well as forming a beautiful landscape.

Mechanisms of Suppression of Matrix Metalloproteinases in UVB-Irradiated HaCaT Keratinocytes of Colored Rice Varieties (UVB에 조사된 HaCaT Keratinocytes에서의 유색미에 의한 Matrix Metalloproteinases 발현억제 메커니즘)

  • Choi, Eun-Young;Lee, Jae-Bong;Kim, Do-Hoon;Kwon, Yong-Sham;Cheon, Jung-Yoon;Lee, Jin-Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.5
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    • pp.562-571
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    • 2017
  • In this study, we investigated the anti-oxidant activities [electron-donating ability (EDA), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability, and reactive oxygen species (ROS) inhibitory activity], anti-wrinkle activities [collagenase inhibitory activity, suppression and/or phosphorylation of matrix metalloproteinases (MMPs), and mitogen-activated protein (MAP) kinase activity], and mRNA expression levels using reverse transcription-polymerase chain reaction (RT-PCR) assay in ultraviolet (UV) B ray ($50mJ/cm^2$)-irradiated human keratinocyte HaCaT cells. Josaengheugchal, Sinneungheugchal (SE), Shintoheug rice, Heugjinju rice, and Heugseol (HE) among colored rice varieties were reported to have excellent antioxidant properties. In the EDA and ABTS radical scavenging assays, extracts of the five colored rice varieties had scavenging activities of 72% at concentrations higher $50{\mu}g/mL$. In the collagenase inhibition assay, ethanol extracts of the five colored rice varieties showed high inhibitory effects of about 60% at concentrations higher $25{\mu}g/mL$. In the ROS inhibition assay, ethanol extracts of HE and SE showed very excellent inhibition efficacies at all concentrations. We determined molecular biological mechanisms of MMPs (MMP-1, -3, -8, and -13) and mitogen-activated protein kinase (MAPK) with HE, and the results show that HE suppressed expression of MMPs and phosphorylation of MAPK and increased expression of pro-collagen type I in UVB-irradiated cells. It was also confirmed by RT-PCR that HE reduced expression of MMPs mRNA. Therefore, these results suggest that HE has anti-wrinkle and collagen production effects and may be used as a material in the development of functional food and cosmetic industries.

Korean Ginseng in "The Veritable Records of King Sejong" (『세종실록』을 통해 본 고려인삼)

  • Joo, Seungjae
    • Journal of Ginseng Culture
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    • v.3
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    • pp.11-37
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    • 2021
  • Korean ginseng is the one of the most famous medicinal herbs globally and has long been a representative item of East Asian trade, including across China and Japan. Since Joseon (1392-1910) ginseng trade was entirely controlled by the state, The Veritable Records of the Joseon Dynasty are a valuable resource that can shed light on the history of the ginseng industry at that time. By studying the subsection "The Veritable Records of King Sejong" (世宗實錄), when ginseng was used even more widely, we assess the purpose and scale of its trade in the 15th century, identify its original listing in the geographical appendix, develop a distribution map, and explore similarities to current ginseng cultivation areas. During the reign of King Sejong (1418-1450), ginseng was sent to China as a tribute 101 times, with a combined weight of 7,060 kilograms, with less than one-third of that amount given to Japan and Okinawa. It was used to cover the travel expenses of foreign envoys and servants, but this can be seen to gradually decrease after the regnal mid-term, primarily due to a decrease in the amount of ginseng being collected. At the time, there were 113 areas of naturally growing ginseng as listed in the records' geographical appendix, including 12 recorded in the 'tributes' category: Yeongdeok-gun, Yeongju, and Cheongsong-gun in Gyeongsangbuk-do; Ulju-gun and Ulsan in Gyeongsangnam-do; Jeongeup, Wanju-gun, and Jangsu-gun in Jeollabuk-do; Hwasun-gun in Jeollanam-do; Goksan-gun and Sinpyeong-gun in Hwanghaebuk-do; Jeongju and Taecheon-gun in Pyeonganbuk-do; and Jaseong-gun and Junggang-gun in Jagang-do. A total of 101 places are recorded in the 'medicinal herbs' category, located throughout the mountains of the eight Joseon provinces, except the islands. In comparison with current ginseng cultivation sites, many of these historical areas are either consistent with or adjacent to contemporary locations. The geographical appendix to "The Veritable Records of King Sejong" was compiled in the early days of the king's reign (1432) when there was a lot of wild ginseng. The appendix is a valuable resource that indicates the possibility of growing ginseng on the Korean Peninsula in the future. The apparently natural habitats in the south, where ginseng is not currently cultivated, could be candidates for the future. Moreover, areas in the north where ginseng has not been grown, except Kaesǒng, could be a good alternative under sustainable inter-Korean exchange should cultivation sites move north due to climate warming.