• Journal of the Korean Magnetic Resonance Society
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• v.11 no.2
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• pp.73-84
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• 2007

MTH1821 (UniProtKB/TrEMBL ID O27849) is a 96-residue hypothetical protein from the open reading frame of Methanobacterium thermoautotrophicum H one of the target organisms of structural genomics pilot project. Proteins which contain conserved sequence compared with MTH1821 have not been discovered yet and the functional and structural information for MTH1821 is not available. Here, we present the sequence-specific backbone resonance using multidimensional heteronuc1ear NMR spectroscopy and propose the secondary structure using GetSBY software. The backbone resonances of N, HN, $C_{\alpha}$, $C_{\beta}$, CO and $H_{\alpha}$ which are necessary for a prediction of secondary structure by GetSBY were assigned about 98% (557/568). The secondary structure of MTH1821 confirmed that it is comprised of four strand regions and two helical regions. This report will provide a valuable resource for the calculation solution structure of MTH1821 and for the other hypothetical protein that is targeted for structural-based functional discovery.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.12
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• pp.1768-1775
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• 2015

Recent major domestic facilities, such as nuclear power plants, many control cables are installed and are degraded by long-term use, but research on deterioration diagnosis is lacking. In the event of a fault in the cable due to deterioration can be developed into a major accident such as the main plant is stopped, so the deterioration diagnostic techniques of high reliability for the cable is required. In this paper, proposes a methodology using a cable resonance that can effectively diagnose the deterioration of the cable. Prior to the test, we developed a setup for stable measuring the characteristics of the cable and it verified the suitable of the measurement set-up in terms of interactivity and reliability, also measured S-parameters applying verified measurement set-up to the cables that deterioration degree is different. Then, we had amplified the difference in resonance frequency between the healthy state and the deteriorated state using connection in a series of measured S-parameters. In a result from the method, we have verified that the more deteriorate the cables is, the more decrease the resonance frequency is. Measured results are justified by inducing the resonance frequency calculation of the cable from the S- parameters represented by the hyperbolic function formula. VNA(Vector Network Analyzer) for S-parameter measurements used in this study is Agilent E5061B and shielded twisted-pair cables was used for deterioration diagnostic test.

• Korean Journal of Radiology
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• v.1 no.3
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• pp.142-151
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• 2000

Objective: To determine the optimal scan timing for contrast-enhanced magnetic resonance angiography and to evaluate a new timing method based on the arteriovenous circulation time. Materials and Methods: Eighty-nine contrast-enhanced magnetic resonance angiographic examinations were performed mainly in the extremities. A 1.5T scanner with a 3-D turbo-FLASH sequence was used, and during each study, two consecutive arterial phases and one venous phase were acquired. Scan delay time was calculated from the time-intensity curve by the traditional (n = 48) and/or the new (n = 41) method. This latter was based on arteriovenous circulation time rather than peak arterial enhancement time, as used in the traditional method. The numbers of first-phase images showing a properly enhanced arterial phase were compared between the two methods. Results: Mean scan delay time was 5.4 sec longer with the new method than with the traditional. Properly enhanced first-phase images were found in 65% of cases (31/48) using the traditional timing method, and 95% (39/41) using the new method. When cases in which there was mismatch between the target vessel and the time-intensity curve acquisition site are excluded, erroneous acquisition occurred in seven cases with the traditional method, but in none with the new method. Conclusion: The calculation of scan delay time on the basis of arteriovenous circulation time provides better timing for arterial phase acquisition than the traditional method.

• Journal of the Korean Magnetic Resonance Society
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• v.1 no.2
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• pp.71-78
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• 1997

The magnetic anisotropy effects of peptide group in structured protein on proton chemical shift have been investigated using trialanine modeling. The structure dependent part of chemical shift of C${\alpha}$H of the second amino acid residue was assumed to come purely from the magnetic anisotropy effects of C=O and C-N bonds of peptide in the direct neighborhood and thus to be dependent on and $\psi$ angle of this dipeptide. A set of dipeptide models with different and $\psi$angles were generated and from these models the chemical shift values were calculated using known algorithm to emphasize the role of parameters used in the equation. Comparison of sets of different parameters resulted in an optimized parameters which could reproduce the statistical chemical shift values observed in proteins with respect ot the secondary conformation.

• Journal of the Korean Magnetic Resonance Society
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• v.10 no.1
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• pp.59-73
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• 2006

Spin-lattice relaxation pathway of $CH_2$ spin system by two dimensional NOESY sequence has been discussed. Two-dimensional spectra governed by dipolar relaxation mechanism were simulated in term of transfer mode, the generalization of conventionally used magnetization mode in one dimension. The transfer matrix directly related to the Redfield relaxation matrix can be constructed by the multiplet of transfer mode. The observable relaxation transfer modes causes to variation of the off-diagonal signal intensity of phase sensitive NOESY spectra from which variable spectral density can be extracted with simple group theoretical calculation. The variation of the J-coupling peak intensity as a function of the mixing time in 2-D spectra for $n-Undecane-5-^{13}C$ and Bromoacetic $2-^{13}C$ acid has been theoretically traced.

• Journal of the Korean Magnetic Resonance Society
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• v.10 no.1
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• pp.96-104
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• 2006

The gene coding for APG8a (At4g21980), a protein from Arabidopsis thaliana, is involved in the autophagy process. The protein is an interesting candidate for structure determination by NMR spectroscopy. Toward this end, APG8a has been produced recombinantly in Escherichia coli and typical NMR experiments such as $^{15}N-HSQC$, HNCA, HN(CO)CA, CBCA(CO)NH, HCCH-TOCSY, HNCO were performed. The backbone resonances, HN, N, CA, CB, and C' were sequence-specifically assigned, and the secondary structures including 3 $\alpha$ helices and $4\beta$ strands were deduced based on the assignments. Due to the intrinsic flexibility or the effect of the denaturant, the backbone resonances were not fully observed. Since the structure calculation by NMR data was not possible, the 3-dimensional model was built based on the sequence homology, and compared with the NMR results. The overall structure of the model could explain and complement the NMR derived secondary structures.

• Journal of the Korean Magnetic Resonance Society
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• v.7 no.1
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• pp.16-24
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• 2003

Nuclei with the spin quantum number not smaller than unity have not only the nuclear magnetic moment but also the electric quadrupole moment. The quadrupole moment couples with the electric field gradient (EFG) to produce the nuclear quadrupole interaction. It is well known that two independent parameters, i.e. the quadrupole coupling constant (QCC) and the asymmetry parameter ($\eta$) together with the principal axis directions can fully describe the interaction and are very sensitive to the local symmetry and structure of the solid. In order to obtain quantitative estimates of the EFG tensor for various simple ionic configurations surrounding the nucleus under consideration, we employ the simple point charge approximation and apply the calculated results to some real crystals. General agreement is rather satisfactory.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.52 no.10
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• pp.484-490
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• 2003

This paper describes with the stator analysis and design of a ring type ultrasonic motor. The design for piezoelectric ceramic and elastic body of stator were calculated by using the finite element method (FEM) that consider the resonance frequency, vibration mode and coupling efficiency. Namely, such results were acquired the calculation result of the piezoelectric ceramic thickness 0.5[mm], elastic body thickness 2.0[mm], resonance frequency 51.8[kHz], vibration mode 7 order and coupling efficient 12.5[%], the outer and inner diameter of vibrator 50[mm], 38[m]. On the basis of such result, the ring type ultrasonic motor was manufactured. Also for driving characteristics of ring type ultrasonic motor, 2-phase inverter was constructed. Then the propriety of this paper was established from comparision of the simulation and an experiment results of the ring type ultrasonic motor.

• Bulletin of the Society of Naval Architects of Korea
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• v.25 no.3
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• pp.3-12
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• 1988

A natural or an artificial harbor can exhibit frequency(or period) dependent water surface oscillations when excited by incident waves. Such oscillations in harbors can cause significant damage to moored ships and adjacent structures. This can also induce undesirable current in harbors. Many previous investigators have studied various aspects of harbor resonance problem. In the percent paper, both a localizes finite element method(LFEM) which is based on the functional constructed by Chen & Mei(1974) and Bai & Yeung(1974) and an integral equation method which was used by Lee(1969) are applied to harbor resonance problem. The present method(LFEM) shows computationally more efficient than the integral equation method. Our test results shows good agreement compared with other results. This enhanced computational efficiency is due to the fact that the present method gives a banded symmetric coefficients matrix and requires much less computational time in the calculation of the influence coefficients matrix than the integral equation method involved with Green's function. To test the present numerical scheme, two models are treated here. The present method(LFEM) can be extended to a fully three dimensional harbor problem with the similar computational advantage.

• Proceedings of the IEEK Conference
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• 2000.07a
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• pp.175-178
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• 2000

The resonance characteristic of microstrip antenna with an airgap between the substrate layer and ground plane is investigated. The study is performed by using a rigorous Green’s function formulation in the spectral domain and Galerkin’s moment method calculation. The numerical convergence using sinusoidal basis functions, the unknown surface current distribution in the rectangular patch, is discussed. Numerical result for the effects of airgap and patch length on the complex resonant frequencies of the rectangular microstrip structure are also presented