• Title/Summary/Keyword: Resin Mixture Ratio

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Characterization of ATPase Activity of Free and Immobilized Chromatophore Membrane Vesicles of Rhodobacter sphaeroides

  • Kim, Hyeonjun;Tong, Xiaomeng;Choi, Sungyoung;Lee, Jeong K.
    • Journal of Microbiology and Biotechnology
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    • v.27 no.12
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    • pp.2173-2179
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    • 2017
  • The intracytoplasmic membrane of Rhodobacter sphaeroides readily vesiculates when cells are lysed. The resulting chromatophore membrane vesicle (CMV) contains the photosynthetic machineries to synthesize ATP by ATPase. The light-dependent ATPase activity of CMV was lowered in the presence of $O_2$, but the activity increased to the level observed under anaerobic condition when the reaction mixture was supplemented with ascorbic acid (${\geq}0.5mM$). Cell lysis in the presence of biotinyl cap phospholipid (bcp) resulted in the incorporation of bcp into the membrane to form biotinylated CMV (bCMV), which binds to streptavidin resin at a ratio of approximately $24{\mu}g$ bacteriochlorophyll a/ml resin. The ATPase activity of CMV was not affected by biotinylation, but approximately 30% of the activity was lost by immobilization to resin. Interestingly, the remaining 70% of ATPase activity stayed constant during 7-day storage at $4^{\circ}C$. On the contrary, the ATPase activity of bCMV without immobilization gradually decreased to approximately 40% of the initial level in the same comparison. Thus, the ATPase activity of CMV is sustainable after immobilization, and the immobilized bCMV can be used repeatedly as an ATP generator.

A study on color characteristics of Multi-color functional Rapid Prototypes Using laser stereolithography (광조형을 이용한 다색 기능성 시작품의 색상특성에 관한 연구)

  • 조진구;정해도;손재혁;임용관
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2000.05a
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    • pp.824-828
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    • 2000
  • As production cycle has become more and more shorter, the demand of rapid prototyping technology has increased largely. There are many methods for rapid prototyping technology, such as SLA. SLS, FDM. INK JET, LOM and so on. Of all methods, SLA has been most widely used for fabricating precision parts. But products manufactured by this method have limitation of single color and single material. So the principal purpose of this study is to overcome the limit of single color product. If the internal structure of manufactured product is visible with multi-color characteristic, it is possible to check easily the designed model with reality. In order to give multi-color characteristic to the product, photocurable resin mixed with pigment is used in this study. First, transparency of photocurable resin without pigment is evaluated, and then color characteristic and curing characteristic of the mixture is evaluated changing mixing ratio. Through the basic experiments, it becomes possible to fabricate multi-color 3D prototype without assembly.

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Improvement of Spatial Resolution in Nano-Stereolithography Using Radical Quencher

  • Park, Sang-Hu;Lim, Tae-Woo;Yang, Dong-Yol;Kim, Ran-Hee;Lee, Kwang-Sup
    • Macromolecular Research
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    • v.14 no.5
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    • pp.559-564
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    • 2006
  • The improvement of spatial resolution is a fundamental issue in the two-photon, polymerization-based, laser writing. In this study, a voxel tuning method using a radical quencher was proposed to increase the resolution, and the quenching effect according to the amount of radical quencher was experimentally investigated. Employing the proposed method, the lateral resolution of the line patterns was improved almost to 100 nm. However, a shortcoming of the quenching effect was the low mechanical strength of polymerized structures due to their short chain lengths. Nano-indentation tests were conducted to evaluate quantitatively the relationship between mechanical strength and the mixture ratio of the radical quencher into the resins. The elastic modulus was dramatically reduced from an average value of 3.015 to 2.078 GPa when 5 wt% of radical quencher was mixed into the resin. Three-dimensional woodpile structures were fabricated to compare the strength between the resin containing radical quencher and the original resin.

Characteristics of Elastic Paving Material Made of Sawdust and Urethane Resin Mixture (톱밥과 우레탄 수지 혼합물로 제조한 탄성 포장재의 특성)

  • Choi, Jae-Jin;Lee, Kwan-Ho;Moon, Seung-Kwon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.6
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    • pp.673-680
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    • 2017
  • Research and commercialization of wood chips-urethane resin mixtures as paving materials for park trails and tourist attractions are underway. The aim of this study was to expand the use of such paving materials to the playgrounds, where vigorous physical movements occur frequently. For this purpose, the physical properties and safety of the paving material, in which some or all of the wood chips(passing through a 10mm sieve and remaining in a 3mm sieve) were replaced with sawdust, were studied experimentally. Strength, elastic modulus, slip resistance, shock absorption and heavy metal content tests were carried out by varying the mixing ratio of urethane resin, sawdust and wood chip. As a result, in the case of wood chip-resin mixtures with mass ratios of the resin to total mass of sawdust and wood chips of 1.0 and 1.2 and having a ratio of sawdust mass to total mass of sawdust and wood chips of 0-0.4, it was found that the properties satisfied KS F 3888-2. On the other hand, in case of using sawdust only as a woody material, the shock absorbability was below standard, and the mass ratio of resin to sawdust required 1.2 or more to ensure the specified tensile strength.

Characteristic Analysis on Mixed Filler of Conservation Materials for Stone Cultural Heritage (석조문화재 보존처리에 사용되는 혼합충전제의 특성분석)

  • Song, Chi-Young;Han, Min-Su;Lee, Jang-Jon;Jun, Byung-Kyu;Do, Min-Hwan
    • Journal of Conservation Science
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    • v.25 no.4
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    • pp.439-450
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    • 2009
  • The purpose of this study is to find out characteristics of the fillers made by epoxy resin (L-30) and filling epoxy resin (L-50) with talc, quartz sand and wollastonite with different mixture ratio of 5%, 50%, 80%, 120%, and 150%. The viscometer and colorimeter were used to measure the viscosity and chromaticity of the fillers. Additionally, IC and SEM were used to reveal characteristics of the filler, and checked ultrasonic wave velocity, compressive strength and contact angle to estimate the stability between the filler and stones which are essential for conservation treatments. The filler mixed with the talc had the lowest value in the ultrasonic wave velocity analysis, and its compressive strength decreased as the mixing rate of talc increased. On the other hand, wollastonite had higher values than others in the ultrasonic wave velocity and the compressive strength regardless of epoxy resin type, also, these values increased as mixing rate increased. The properties of the filler, which include the granularity and shape, have influence on characteristics of the stone conservation adhesives. Thus, the filler type, characteristic, and mixture ratio must be considered for effective conservation treatment.

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A Study on Structures and Properties of Liquid Crystal-UV Curable Resin Composite Materials (액정-UV경화 이크릴레이트 수지 복합재료의 구조와 물성에 관한 연구)

  • 김종원
    • Journal of the Korean Graphic Arts Communication Society
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    • v.18 no.1
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    • pp.59-69
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    • 2000
  • The characteristics of liquid crystal polymer composite(LCPC) films are possessed of large-area and flexible display, polarizer free, high contrast, wide angle of visual filed and high responsiveness. It is well known that the LCPC films consisting of a continuous LC phase embeded in a three-dimentional network of polymer matrix are formed by photopolymerization-induced phase separation. In this study, we have investigated the point that both liquid crystals and polymer having different properties have to coexiste as composed films. The purpose of this study has been the development of new application with liquid crystals and UV-curable monomers. In the results abtained on the miscibility of nematic liquid crystal and UV-curable resins, difunctional monomer HX-620 turned out to shows the best. From the results abtained on structures, electro-optical properties and dynamic visocoelasticity for LCPC films, the best mixing ratio of monomer to LC mixture were 3/7(photoinitiator; 4wt%) by weight, and this ratio has been provided the most thermal stability for LCPC films. In the results abtained on structure and discoloration properties of LCPC films, it has been demonstrated that consiste of a 8:2 mixture of chiral nematic liquid crystal and HX-620 has the greatest domain and it was the best discoloration.

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Experimental investigation of the effect of the addition of Aerosil 200 nanoparticles on the water absorption of polymer concrete

  • A.M. Fattahi;Babak Safaei;Elham Moaddab;Zahra Pezeshki
    • Advances in nano research
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    • v.14 no.1
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    • pp.81-92
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    • 2023
  • In this work, the effect of the addition of Aerosil 200, an insulating resin applied in many industries, on the water absorption of cement plast mixture has been experimentally evaluated. First, the preparation stages of cement plast mixture was evaluated based on corresponding standards and then, the effect of the addition of Aerosil 200 nanoparticles (NPs) to cement plast mixtures with sand weight percentage range of 0-0.1% on the variation of water absorption properties was evaluated based on National Standard Institution of Iran 20185 for Concrete Flooring Blocks - Requirements and Test Procedures. Based on the obtained results, it could be found that excessive addition of NPs did not affect the physical properties of the final product. Water absorption percentage was increased in the weight percentage of cement. By the increase of the amount of Aerosil 200 NPs in the prepared cement plast mixture, the percentage of water absorption in weight percentage of sand was decreased. Cement plast with NPs presented significantly lower water absorption than that without NPs.

Preparation of Highly Tough Ethylene Vinyl Acetate (EVA) Heterogeneous Cation Exchange Membranes and Their Properties of Desalination

  • Kim, In Sik;Ko, Dae Young;Canlier, Ali;Hwang, Taek Sung
    • Korean Chemical Engineering Research
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    • v.56 no.3
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    • pp.361-369
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    • 2018
  • A manufacturing method has been devised to prepare novel heterogeneous cation exchange membranes by mixing ethylene vinyl acetate (EVA) copolymers with a commercial cation exchange resin. Optimum material characteristics, mixture ratios and manufacturing conditions have been worked out for achieving favorable membrane performance. Ion exchange capacity, electrical resistance, water uptake, swelling ratio and tensile strength properties were measured. SEM analysis was used to monitor morphology. Effects of vinyl acetate (VA) content, melt index (MI) and ion exchange resin content on properties of heterogeneous cation exchange membranes have been discussed. An application test was carried out by mounting a selected membrane in a membrane capacitive deionization (MCDI) system to investigate its desalination capability. 0.92 meq/g of ion exchange capacity, $8.7{\Omega}.cm^2$ of electrical resistance, $40kgf/cm^2$ of tensile strength, 19% of swelling ratio, 42% of water uptake, and 56.4% salt removal rate were achieved at best. VA content plays a leading role on the extent of physical properties and performance; however, MI is important for having uniform distribution of resin grains and achieving better ionic conductivity. Overall, manufacturing cost has been suppressed to 5-10% of that of homogeneous ion exchange membranes.

An Experimental Study on the Bending Behavior of F.R.P. Sandwich Structure with 2nd Reinforced Bonding (2차 접착된 Sandwich 구조의 굽힘에 관한 실험연구)

  • Kim, Ik Tai
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.19 no.1
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    • pp.47-51
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    • 2016
  • It has made a special study of bending behavior of F.R.P. sandwich beams with bonded 2nd-reinforced plies. Specimen's faces were made of chopped mat 300-450, roving clothes 570, core is urethane foam, resin is 713bp unsaturated polyester for ship construction and the mixture weight ratio of resin versus fiber was 55:45 for bending analysis. The purpose of this paper is to study the exact bending behavior of bonded area's deflection and stiffness depends upon various bonded F.R.P. (2nd reinforced ply) length and thickness on which covered joints and to find the optimum design for the sandwich structures. All results and suggestions are based on experiment and using thick face calculation.

Impact Echo Test for the Dynamic Characteristics of a Vibration-Mitigated Concrete Structure

  • Chung, Young-Soo;Park, Young-Goo
    • KCI Concrete Journal
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    • v.14 no.1
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    • pp.23-29
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    • 2002
  • Recent construction activities have given rise to civil petitions associated with vibration-induced damages or nuisances. To mitigate unfavorable effects of construction activities, the measures to reduce or isolate from vibration need to be adopted. In this research, a vibration-mitigated concrete, which is one of the active measures for reducing vibration in concrete structures, was investigated. Concrete was mixed with vibration-reducing materials (i.e. latex, rubber power, plastic resin, and polystyrofoam) to reduce vibration and tested to evaluate dynamic material properties and structural characteristics. Normal and high strength concrete specimens with a certain level of damage were also tested for comparisons. In addition, recycling tires and plastic materials were added to produce a vibration-reducing concrete. A total of 32 concrete bars and eight concrete beams were tested to investigate the dynamic material properties and structural characteristics. Wave measurements on concrete bars showed that vibration-mitigated concrete has larger material damping ratio than normal or high strength concrete. Styrofoam turned out to be the most effective vibration-reducing mixture. Flexural vibration tests on eight flexural concrete beams also revealed that material damping ratio of the concrete beams is much smaller than structural damping ratio for all the cases.

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