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Validation of Reference Genes for Quantitative Real-Time PCR in Bovine PBMCs Transformed and Non-transformed by Theileria annulata

  • Zhao, Hongxi;Liu, Junlong;Li, Youquan;Yang, Congshan;Zhao, Shuaiyang;Liu, Juan;Liu, Aihong;Liu, Guangyuan;Yin, Hong;Guan, Guiquan;Luo, Jianxun
    • Parasites, Hosts and Diseases
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    • 제54권1호
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    • pp.39-46
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    • 2016

  • Theileria annulata is a tick-borne intracellular protozoan parasite that causes tropical theileriosis, a fatal bovine lymphoproliferative disease. The parasite predominantly invades bovine B lymphocytes and macrophages and induces host cell transformation by a mechanism that is not fully comprehended. Analysis of signaling pathways by quantitative real-time PCR (qPCR) could be a highly efficient means to understand this transformation mechanism. However, accurate analysis of qPCR data relies on selection of appropriate reference genes for normalization, yet few papers on T. annulata contain evidence of reference gene validation. We therefore used the geNorm and NormFinder programs to evaluate the stability of 5 candidate reference genes; 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ACTB (${\beta}-actin$), PRKG1 (protein kinase cGMP-dependent, type I) and TATA box binding protein (TBP). The results showed that 18S rRNA was the reference gene most stably expressed in bovine PBMCs transformed and non-transformed with T. annulata, followed by GAPDH and TBP. While 18S rRNA and GAPDH were the best combination, these 2 genes were chosen as references to study signaling pathways involved in the transformation mechanism of T. annulata.

  • https://doi.org/10.3347/kjp.2016.54.1.39 인용 PDF KSCI

3차 교차상관신호의 역상관을 통한 펨토초 및 처프된 피코초 펄스의 정확한 선행펄스 대비율 측정 (Accurate Contrast-ratio Measurement of Femtosecond and Chirped Picosecond Pulses from the Decorrelation of Third-order Correlation Trace)

  • Hong, Kyung-Han;Sung, Jae-Hee;Yu, Tae-Jun;Choi, Il-Woo;Kim, Hyung-Taek;Noh, Young-Chul;Ko, Do-Kyeong;Lee, Jong-Min
    • 한국광학회:학술대회논문집
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    • 한국광학회 2007년도 동계학술발표회 논문집
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    • pp.349-350
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    • 2007

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