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Molecular Cloning of a Cellulase Gene from Abalone Haliotis discus hannai and Its Expression in E coli

  • Park, Eun-Mi;Han, Yun-Hee;Park, In-Suk;Nam, Bo-Hye;Kong, Hee Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Young-Ok
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.108-112
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    • 2007

  • A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

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EFFECT OF 17 $\beta$-ESTRADIOL ON MATING BEHAVIOR AND REPRODUCTIVITY OF MALE MEDAKA (ORYZIAS LATIPES)

  • Jisung Ryu;Lee, Chul-Woo;Park, Eung-Roh;Nam, Seong-Sook;Nam, Kyu-Chan;Rhu, Hong-Il;Jeon, Seong-Hwan;Na, Jin-Gyun;Park, Kwangsik
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2001.05a
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    • pp.135-135
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    • 2001

  • Mating behavior and reproductivity of male fish were studied as an in vivo screening method of endocrine disruptors. Male medaka (Oryzias latipes) were exposed to 17$\beta$-estradiol at nominal concentrations of 2 and 20 $\mu\textrm{g}$/L for 14 days.(omitted)

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