• Journal of Ginseng Research
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• v.46 no.2
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• pp.255-265
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• 2022

Background: Ginsenoside Rb1, a bioactive component isolated from the Panax ginseng, acts as a remedy to prevent myocardial injury. However, it is obscure whether the cardioprotective functions of Rb1 are related to the regulation of endogenous metabolites, and its potential molecular mechanism still needs further clarification, especially from a comprehensive metabolomics profiling perspective. Methods: The mice model of acute myocardial ischemia (AMI) and oxygen glucose deprivation (OGD)-induced cardiomyocytes injury were applied to explore the protective effect and mechanism of Rb1. Meanwhile, the comprehensive metabolomics profiling was conducted by high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS) and a tandem liquid chromatography and mass spectrometry (LC-MS). Results: Rb1 treatment profoundly reduced the infarct size and attenuated myocardial injury. The metabolic network map of 65 differential endogenous metabolites was constructed and provided a new inspiration for the treatment of AMI by Rb1, which was mainly associated with mitophagy. In vivo and in vitro experiments, Rb1 was found to improve mitochondrial morphology, mitochondrial function and promote mitophagy. Interestingly, the mitophagy inhibitor partly attenuated the cardioprotective effect of Rb1. Additionally, Rb1 markedly facilitated the phosphorylation of AMP-activated protein kinase α (AMPKα), and AMPK inhibition partially weakened the role of Rb1 in promoting mitophagy. Conclusions: Ginsenoside Rb1 protects acute myocardial ischemia injury through promoting mitophagy via AMPKα phosphorylation, which might lay the foundation for the further application of Rb1 in cardiovascular diseases.

• Analytical Science and Technology
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• v.28 no.4
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• pp.278-287
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• 2015

Phosphodiesterase type 5 inhibitors (PDE-5 inhibitors) are used in the treatment of erectile dysfunction. In recent years, a number of reports have been conducted on dietary supplements contaminated with PDE-5 analogues. In this study, 58 analogues of PDE-5 inhibitors were sorted into five groups: tadalafil, sildenafil, hongdenafil, vardenafil, and other analogues. These analogues were then evaluated using a liquid chromatography-quadrupole-time of flight mass spectrometry (LC-QTOF-MS) electrospray ionization mass method. Each compound has a unique fragmentation ion, which can be easily analyzed qualitatively. The fragmentation pathways of the analogues were elucidated based on the QTOF-MS and MS/MS data. Common ions were confirmed for each group by analyzing the structural characteristics and fragmentation pathways. Specifically, common ions were observed at m/z 169.08 and 135.04 (tadalafil analogues), m/z 311.15 and 283.12 (sildenafil analogues and hongdenafil analogues), and m/z 312.16 and 151.09 (vardenafil analogues). The advantage of this method is that the structure of unknown components can be determined by interpreting the product ions. Hence, the developed method can be used for the identification of unknown compounds. Fragmentation pathways may also aid in the detection and identification of PDE-5 inhibitor analogues.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.1
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• pp.87-98
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• 2012

The Ministry of Education, Science and Technology has been implementing the government's recognition project regarding the program accrediting organizations in Higher Education. This research is to establish an evaluation model for the government's recognition process of the program accrediting organizations, and develop the evaluation categories, items and indices for the model. The research has set the concept for the government's recognition regarding the program accrediting organizations and designed an evaluation model for such organizations. The evaluation model embraces a preliminary evaluation process, assessing an eligibility of the accrediting organizations for the higher education based on the formalities set forth for the government recognition organization accrediting process. It also includes the main evaluation process, which assess the eligibility based on the evaluation standards stipulated in the associated legislation and regulations. In addition, through in-depth verification processes by experts, this research finalized 2 evaluation domains, 5 evaluation categories, 24 evaluation items and 33 evaluation indices.

• International Journal of Computer Science & Network Security
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• v.21 no.5
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• pp.243-255
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• 2021

This study aimed to identify the effectiveness of the use of distance-evaluation tools and methods among students with learning difficulties from the teachers' point of view, to achieve this goal. A scale was built, and the psychometric characteristics were validated. It consisted, in its final form, of 17 items distributed on four axes, in addition to three open questions. It was applied to a random sample of (149) teachers of students with learning difficulties in Makkah Region. The results showed that teachers' keenness to encourage students with learning difficulties, so that they would not feel frustrated with the distance learning process. It was also evident that teachers did not use achievement portfolios in the evaluation process. In connection with the appropriate evaluation methods, the majority indicated the use of work sheets and visual evaluation methods that rely on audio and visual skills, such as presenting videos, pictures, audio and games, and applying short objective tests. Among the proposals to improve evaluation methods and tools: Individual evaluation, attention to individual treatment, obligating personal attendance of students to school, splitting the required tasks, and not increasing the skills required to be mastered. As for the obstacles that teachers face: Lack of time, difficulty in communicating with students with distance learning difficulties and problems related to the Internet such as interruption, weakness, or lack of availability.

• Biomolecules & Therapeutics
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• v.25 no.3
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• pp.288-295
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• 2017

The incidence of polypharmacy-which can result in drug-drug interactions-has increased in recent years. Drug-metabolizing enzymes and drug transporters are important polypharmacy modulators. In this study, the effects of bosentan and rifampin on the expression and activities of organic anion-transporting peptide (OATP) and cytochrome P450 (CYP450) 2C9 and CYP3A4 were investigated in vitro. HEK293 cells and primary human hepatocytes overexpressing the target genes were treated with bosentan and various concentrations of rifampin, which decreased the uptake activities of OATP transporters in a dose-dependent manner. In primary human hepatocytes, CYP2C9 and CYP3A4 gene expression and activities decreased upon treatment with $20{\mu}M$ $bosentan+200{\mu}M$ rifampin. Rifampin also reduced gene expression of OATP1B1, OATP1B3, and OATP2B1 transporter, and inhibited bosentan influx in human hepatocytes at increasing concentrations. These results confirm rifampin- and bosentan-induced interactions between OATP transporters and CYP450.

• Geomechanics and Engineering
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• v.12 no.1
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• pp.35-52
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• 2017

In order to evaluate the grouting effects of water-rich fault in tunnels systematically, a feasible and scientific method is introduced based on the extension theory. First, eight main influencing factors are chosen as evaluation indexes by analyzing the changes of permeability, mechanical properties and deformation of surrounding rocks. The model of evaluating grouting effects based on the extension theory is established following this. According to four quality grades of grouting effects, normalization of evaluation indexes is carried out, aiming to meet the requirement of extension theory on data format. The index weight is allocated by adopting the entropy method. Finally, the model is applied to the grouting effects evaluation in water-rich fault F4-4 of Qingdao Jiaozhou Bay Subsea Tunnel, China. The evaluation results are in good agreement with the test results on the site, which shows that the evaluation model is feasible in this field, providing a powerful tool for systematically evaluating the grouting effects of water-rich fault in tunnels.

• Korean Management Science Review
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• v.14 no.1
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• pp.177-203
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• 1997

This study concerns performance evaluation of the research-funding agencies focused on Korea Science and Engineering Foundation(KOSEF). On the basis of the constituents approach and the systems approach of organizational effectiveness theory, the evaluation items are derived reflecting generic characteristics of the research-funding agencies, and their priorities are determined using analytic hierarchy process(AHP). Three groups of constituents with differing interest and expections are identified, and their perceptions on the importance of the evaluation items are measured through questionnaire survey. Analyses show that the evaluation items perceived important by each constituency are significant different from one another. The implications of the result to the Government and KOSEF are discussed.

• ETRI Journal
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• v.27 no.4
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• pp.465-468
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• 2005

This paper presents a new trust evaluation scheme in an ad hoc network. To overcome the limited information about unfamiliar nodes and to reduce the required memory space, we propose a cluster-based trust evaluation scheme, in which neighboring nodes form a cluster and select one node as a cluster head. The head issues a trust value certificate that can be referred to by its non-neighbor nodes. In this way, an evaluation of an unfamiliar node's trust can be done very efficiently and precisely. In this paper, we present a trust evaluation metric using this scheme and some operations for forming and managing a cluster. An analysis of the proposed scheme over some security problems is also presented.

• Analytical Science and Technology
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• v.16 no.1
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• pp.90-93
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• 2003

The standard uncertainties of two different approaches using the same data set are compared and evaluated using an example of gravimetric standard gas preparation. It is shown that the correlation between input quantities should be taken into account for the proper evaluation of uncertainty resulting from random effects.

• International Journal of Advanced Culture Technology
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• v.6 no.4
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• pp.131-142
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• 2018

This research examines the impact of teachers' self-evaluation on their classroom interactions with students. Changes in class behavior were analyzed by following two experienced middle school teachers for one semester of self-evaluation, comparing their classes at the beginning and end of the semester. A qualitative research method was adopted to take, a deeper look at the effect of changes in the teachers' in class actions. Both of the teachers reported positive effects of implementing self-evaluation on their teaching pedagogy, focusing specifically on their interactions with students when asking question and giving feedback. By the end of semester, they were asking broader questions that encouraged their students to engage in deeper thought and, when giving feedback, the class atmosphere was much brighter with better communication due to the positive reinforcement provided. This research supports research into ways to enhance teachers' expertise and improve their teaching via self-evaluation, centering on teachers' actions in the classroom.