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Difference in Physiological Responses to Environmental Stress in Protox Inhibitor Herbicide-Resistant Transgenic Rice and Non-transgenic Rice (Protox 저해형 제초제 저항성 형질전환벼와 비형질전환벼의 환경스트레스에 대한 생리적 반응 차이)

  • Yun, Young-Beom;Kwon, Oh-Do;Shin, Dong-Young;Hyun, Kyu-Hwan;Lee, Do-Jin;Jung, Ha-Il;Kuk, Yong-In
    • Korean Journal of Weed Science
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    • v.32 no.1
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    • pp.35-43
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    • 2012
  • The objective of this research was to confirm the difference in physiological responses to environmental stresses such as chilling, high temperature, NaCl, and chemical stress (paraquat) in Protox inhibitor resistant-transgenic rice (MX, PX, and AP37) and its non-transgenic counterpart (WT). Transgenic and non-transgenic rice plants were exposed to a chilling temperature of $5^{\circ}C$ for 1 day or a high temperature of $45^{\circ}C$ for 4 days and allowed to recover at $25^{\circ}C$ for 6 days after the chilling treatment or 8 days after the high temperature treatment. Leaf injury, shoot fresh weight, porphyrin biosynthesis substances, and chlorophyll content were investigated in transgenic and non-transgenic rice at 6 days after 0.5% and 1% NaCl treatments or at 5 days after 0~300 ${\mu}M$ paraquat treatments. No significant difference in leaf injury and shoot fresh weight were observed between transgenic and non-transgenic rice during chilling and recovery. Plant height and shoot fresh weight were also similar between transgenic and non-transgenic rice during the high temperature and recovery period (0~5 days). However, plant height and shoot fresh weight in transgenic rice line MX and PX were lower than in non-transgenic rice at 6 days for recovery. Leaf injury, chlorophyll, and Mg-Proto IX ME contents had no significant difference between transgenic rice and non-transgenic rice after NaCl treatment, but Proto IX content for AP37 and shoot fresh weight for PX and AP37 in 0.5% NaCl treatment were significantly reduced compared with non-transgenic rice. There was no difference in leaf injury and shoot fresh weight when comparing transgenic rice and non-transgenic rice after paraquat treatment. Although transgenic rice and non-transgenic rice showed a little difference at a particular measurement period in certain environmental stresses, there was generally no difference in physiological responses between transgenic rice and non-transgenic rice.

A Study on Establishment for Archival Management and Training of Archivists (기록관리학의 정립과 기록전문가 양성교육에 관한 연구)

  • Choe, Jung-Tai;Yoon, Song-Won
    • Journal of Korean Society of Archives and Records Management
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    • v.1 no.1
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    • pp.95-129
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    • 2001
  • The treatise is a follow-up thesis of "The Archival Management : destination of its education" published by The Research Institute for Korean Archives and Records in June 17, 2000, and it was written to secure its original temper of the "Archival Management" which has established at first as the domestic collegiate educational course. Presently, we are mixed up for interpretation of terminology by facing unfamiliar words: such as, Archives, Documents, Manuscripts, Records / Archival Management, Archival Preservation / Archivist, Archival Conservator, Manuscripts Curator and Record Manager. Also, tile treatise has confirmed scholars' various opinion about its education and curriculum not only domestic but overseas scholars, and examined realities and course of studies of 4 overseas countries, and also have purpose to pursue domestic universities' actual condition of curriculum and its reform measure. And since dispositon of Archivist and the professional organization of the Archivist was based on the Laws, therefore, establishment of an educational institution for Archival Management from now on will be expanded more and need to be accelerated. Accordingly, the universities that already established or to be established from now on, shall be needed educational quality and its contents to be ascended. Its concrete plan is as follows : (1) Now, disarranged archive and record words, and to make publish 'Glossary of Archives & Records' as soon as possible. (2) Remind the substance of the Record and Archival Management and its sphere again and need to be considered alteration of the studies' name. (3) There needs to establish the role and establishment of conception of Archivists. (4) Refer to the theory and practical educational method of overseas professional Archivist Scholars, but to be needed to develop the curriculum which is accorded with am tradition and way of thinking. (5) Confer for development of teaching materials and educational method jointly through 'Academic Society', and there needs reorganize the subject which is fit for each university's characteristics. (6) Recently, "Cultural Resources Studies" for research specialization which has established in Graduate School of Tokyo University was very useful to us. We also need to be considered such establishment of process.

Seed Viability and Growth Characteristics of Eclipta prostrata (L.) L. (한련초의 종자생존력(種子生存力) 및 생장특성(生長特性))

  • Lee, H.K.;Moody, K.
    • Korean Journal of Weed Science
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    • v.8 no.3
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    • pp.309-316
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    • 1988
  • Several experiments were conducted to investigate the achene viability and growth characteristics of Eclipta prostrata (L.) L. No dormancy and no after-ripening requirement were found for E. prostrata achenes. When achenes were stored at room temperature, germination did not decrease with up to 5 months storage. Large differences in loss of viability of E. prostrata achenes occurred when different dehydration methods were used. Immediate dehydration resulted in high viability, but slow dehydration resulted in severe loss of viability. Achene viability at shallow burial depths (5 and 10 cm deep) was lower under upland soil conditions than under lowland soil conditions. Seedling growth was greatly reduced when flooding to a depth of 10 cm occurred at or before the 4-leaf stage. Flooding after the 4-leaf stage stimulated stem elongation. Branching started from the second week and usually terminated at the tenth week. Leaf size was determined by the branch which are related to the assimilate supply. Flowering of E. prostrata started during the fifth week after emergence, and mature achenes were produced from the sixth week. Ten to 14 days were needed for the achenes to mature. About 14,000 achenes were produced on each plant. Achene production per week increased from the sixth week to the tenth week and thereafter it declined. The average number of achenes per inflorescence decreased with delay in flowering.

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Adaptation Strategy in Dry Matter and Seed Production of Rice and Weed Species (벼와 잡초(雜草)의 건물생산(乾物生産) 및 종자생산(鐘子生産) 특성(特性)에 따른 환경적응(環境適應) 전략(戰略))

  • Kim, Soon-Chul;Moody, Keith
    • Korean Journal of Weed Science
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    • v.9 no.3
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    • pp.183-200
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    • 1989
  • An experiment was conducted at the greenhouse of the International Rice Research Institute in 1987 to find out the character of dry matter production, the potential seed production ability and the ecological and physiological strategies to adaptation. For these, two rice cultivars, IR64(lowland rice) and UPLRi-5(upland rice), and seven weed species were used ; Echinochloa glabrescens Munro ex Hook. f., E. cress-galls ssp. hispidula (Retz.) Honda, E. colons (L.) Link, Monochoria vaginalis (Burin. f.), Ludwigia octovalvis Jacq.) Raven, Fimbristylis miliacea Vahl and Cyperus difformis L. Adaptation strategies of weed species varied by species. However, they had efficient seed production strategy through different ways. In general, sedge weed species (F. miliacea and C. difformis) produced great amount of seeds at the expense of seed size through greater ratooning ability and low relative dry weight for flowering. For broadleaved weed species, greater number of descendants were obtained through high plasticity and low relative dry weight for flowering (M. vaginalis) or greater growth ability through effective photosynthetic efficiency (L. octovalvis). Grass weed species, on the other hand, produced their seeds through effective growth (net assimilation rate and relative growth rate), high ratooning ability (except E. crus-galls ssp. hispidula) or low relative dry weight to maximum dry weight for flowering (E. glabrescens). The harvest indices of the weed species were considerably lower than those of rice. Fimbristylis miliacea had the greatest ratooning ability followed by C. difformis, E. colons and E. glabrescens. The greatest seed productivity was recorded by C. difformis (279, 000) and L. octovalvis (268,000) while rice produced the least number of seeds (1300-6100). Log seed weight had a negative linear relationship with log seed number (y=6.30-1.48X, $R=-0.965^{**}$). For all species plant plasticity response was not directly correlated with mortality response.

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Effect of Rice Bran and Barley Bran Application on Growth and Yield of Chinese Chive (Allium tuberosum Rottler) and Taro (Colocasia esculenta) and Weed Control (쌀겨, 보릿겨 처리가 부추와 토란의 생육과 수량 및 잡초방제에 미치는 영향)

  • Ryu, Deok-Kyo;Yun, Young-Beom;Kwon, Oh-Do;Shin, Dong-Young;Hyun, Kyu-Hwan;Lee, Do-Jin;Kuk, Yong-In
    • Korean Journal of Weed Science
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    • v.31 no.3
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    • pp.260-270
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    • 2011
  • This study was carried out to examine the effect of rice bran, barley bran, burned rice bran, and burned barley bran on the growth and yield of Chinese chive (Allium tuberosum Rottler), taro (Colocasia esculenta), and weed control. When the above 4 brans were examined 13, 27, 41 and 57 days respectively after application, the plant height of Chinese chive applied with burned barley bran was significantly higher than non-treated control, whereas the other brans did not have any distinct effect on the plant height or population number of Chinese chive. However, when examined 57 days after the application of the above 4 brans, all the plants applied with brans showed more than twice the improvement in shoot fresh weight compared with non-treated control. A chemical analysis of soil 57 days after the application of the above 4 brans showed that the soils were richer in available phosphate and organic matter. Shoot fresh weight of Chinese chive at 2 weeks after cutting was significantly higher in barely bran treated plot than in non-treated plot. In the case of taro, only taro plots transplanted when 10 cm tall and applied with barley bran showed an improvement in growth increment of both the underground and above parts. However, when sowed seeds after the application of the 4 brans, the yield of taro was reduced by the brans. Thus this research indicates that the effect of brans is differ based on the amount of bran application as well as crops. The effect of weed control on Echinochloa crus-galli, Digitaria clliaris, Chenopodium album, and Solanum nigrum as affected by brans was very low in pot conditions. Weed efficacy of the brans was also very low in field conditions. Growth of Chinese cabbage and garland chrysanthemum was inhibited 63% and 37% by rice bran at $4,000kg\;ha^{-1}$, respectively, but other crops such as maize, squash, cucumber, and Chinese chive were inhibited by 0-20%. These results were similar to that of barley bran except for Chinese cabbage.

Germination of Two Rice Cultivars and Several Weed Species (벼와 수종(數種) 논잡초(雜草)의 휴면성(休眠性)과 발아성(發芽性))

  • Kim, Soon-Chul;Moody, Keith
    • Korean Journal of Weed Science
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    • v.9 no.2
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    • pp.116-122
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    • 1989
  • An experiment was carried out at the International Rice Research Institute in 1987 to understand the seed dormancy and germination habit of rice and several weed species. The germinability of the weed seeds just after harvest was variable depending on the species and ranged from 0 to 72%. Two rice cultivars, IR64(lowland type) and UPLRi-5(upland type) had higher than 95% in germination ability throughout the experimental period due to the fact that the rice seeds came from the harvest of the previous season and dormancy had already been overcome. The length of the storage period needed to overcome dormancy at room temperature($25{\pm}2^{\circ}C$) was about 50 days for Echinochloa glabrescens Munro ex Hook, f., more than 60 days for E. crus-galli ssp. hispidula (Retz.) Honda and 20 days for Ludwigia octovalvis(Jacq.) Raven. Seeds of E. colona(L.) Link, Monochoria vaginalis(Burm. f.) Presl, Fimbristylis miliacea(L.) Vahl and Cyperus difformis L. appeared to have no dormancy. Among the nine species M. vaginalis had the lowest germination of less than 1% throughout the experimental period. However, its seed germinated easily when planted in soil. The low germinability of E. glabrescens, E. crus-galli ssp. hispidula and L. octovalvis just after harvest could be overcome through pretreatment of seeds either by soaking in nitric acid(0.1N) for 1 day or removal of the hull in the grass species, the nitric acid treatment being superior. The results imply that germination habit of weed species varied depending on the species through their differential dormancy period or differential germination strategy.

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Germination and Seedling Development of Rice and Echinochloa Species (벼와 피의 발아(發芽) 및 유묘발달(幼苗發達) 양상(樣相))

  • Kim, Soon-Chul;Moody, Keith
    • Korean Journal of Weed Science
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    • v.9 no.2
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    • pp.108-115
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    • 1989
  • An experiment was carried out at the International Rice Research Institute in 1987 to understand the seed germination and seedling development of rice and Echinochloa species. The percentage germination of rice cultivars(IR64 for lowland-type and UPLRi-5 for upland-type) was extremely high(>90%) regardless of temperature while that of Echinochloa species ranged from 10 to 80% depending on the species. Among these, E. crus-galli ssp. kispidula had the lowest germination with less than 20% at the high temperatures ($30^{\circ}C$, $35^{\circ}C$) and about 45% at the low temperature ($25^{\circ}C$). Original seed weight gradually decreased with time white root and shoot weight increased for both rice and Echinochloa species. However, increase in root and shoot weight did not compensate for the loss of stored carbohydrate during the experimental period. The root of E. glabrescens grew from the seed on the opposite side of the shoot while for rice it grew from the same side of the seed as the shoot. Echinochloa glabrescens had a leaf blade-like expanded first leaf that contained chlorophyll while rice had an incomplete first leaf that had no leaf blade and no chlorophyll. Due to this E. glabrescens could grow independently 2 to 3 days earlier than rice.

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Studies on Photosynthetic and Respiratory Characteristics in Warm Season and Cool Season Turfgrasses (한지형(寒地型) 잔디와 난지형(暖地型) 잔디의 광합성(光合成) 및 호흡특성(呼吸特性))

  • Nan, Xuan Song;Kaneko, Seiji;Ishii, Ryuichi
    • Korean Journal of Weed Science
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    • v.15 no.2
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    • pp.166-174
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    • 1995
  • This experiment was conducted to investigate a cause of summer depression of cool season turfgrass, using nine cultivars in warm season and twenty-eight cool season turfgrasses. Even though an average of apparent photosynthesis(APS) per fresh weight was 13.09 mg $CO_2$/g/h in warm season turfgrass and 7.75 mg $CO_2$/g/h in cool season turfgrass, the Creeping bentgrass in cool season type was higher than Kikuyugrass and Bahiagrass in warm season type. The optimum temperature for the heighest APS was $30^{\circ}C$ in warm season type and $25^{\circ}C$ in cool season type. In $CO_2$ compensation point(CCP) as an index of dark respiration, it was higher in cool season turfgrass(75.6ppm) than warm season turfgrass(29.5ppm). In warm season type, even though the temperature increased from $25^{\circ}C$ to $40^{\circ}C$ the CCP was not increased. But the higher temperature rises the more increased CCP in cool season type. Dark respiration(DR) was higher in cool season type than warm season type under various temperature conditions, but the increasing ratio of DR with the temperature increment was not so much differed between two types.

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The Collection of Paintiongs and Calligraphy at Jipgyeong-dang Hall during King Gojong's Reign(1897~1907) (고종연간 집경당(緝敬當)의 운용과 궁중(宮中) 서화수장(書畵收藏))

  • Hwang, Jung-yon
    • Korean Journal of Heritage: History & Science
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    • v.40
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    • pp.207-241
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    • 2007
  • This paper investigates the royal collection of paintings and calligraphy during King Gojong's (高宗) reign (1897~1907) based on the function of the Jipgyeong-dang Hall (緝敬堂), situated at "sleeping realm (寢殿)," Gyeongbok Palace. Using the surviving palace records and art works this study argues that the date of building the hall is approximately confined to the year of 1890. Not being matched with the general opinion that the Jipgyeong-dang Hall was used for the official meeting with envoys, this hall functioned as the main place for royal audience and the storehouse for archives. The role of Gojong as collector and patron was essential not only to the maintenance of the collection but also to the strengthening of royal authority just before the Japanese annexation in 1910. The specific titles of the collection at this hall can be verified through the Catalogue of the Books, Paintings, and Calligraphy Exposed to the Sun at Jipgyeong-dang Hall (緝敬堂曝曬書目) dating to the nineteenth century. The records of the catalogue inform us that more than 1,000 paintings and pieces of calligraphy, inkrubbings from old steles, manuals for painting, and encyclopedia concerning art theories from Korea, China, and Japan were preserved there. The collection of Jipgyeong-dang Hall resulted from Gojong's policy to foster the collection of contemporary Chinese and Japanese art works and various catalogues. Standing behind the Gyeongbok Palace, the Jibok-jae Hall (集玉齋) also preserved the diverse sources of practical learning, as did the Jipgyong-dang Hall for Gojong. The enormous royal collections by Gojong might have been constructed in accordance with the royal artistic taste and the artistic milieu of the late Joseon period. The surviving royal catalogues confirm this assumption as documentary evidence.

Weed Occurrence in Upland Crop Fields of Korea (최근(最近) 한국(韓國)의 전작지(田作地) 잡초발생(雜草發生) 분포(分布)에 관(關하)여)

  • Chang, Y.H.;Kim, C.S.;Youn, K.B.
    • Korean Journal of Weed Science
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    • v.10 no.4
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    • pp.294-304
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    • 1990
  • For the survey of weed distribution in the cultivated upland of Korea, weed species were investigated at 2 field by crop of 2 myon per kun in 81 kun selected among the 139 kun of the whole country. 232 species in 46 families were observed, totally. From among the result, 165 species in 39 families the in winter crop field, 189 species in 41 families in the summer crop field were classified. 122 species in 34 families were emerged the from the upland crop field of the whole season. Further more, in the 10 dominant weed species which emerged from upland crop field, Alopecurus aequalis, Chenopodium album, slellaria media, Galium spurieum, Capsella bursa-pastoris and Rorippa islandica were dominated in the winter upland and paddy field, and that Erigeron canadensis, Cyperus amuricus, Equisetum arvense and Arenaria serpyllifolia were dominated in the winter upland field, additionally. Stellaria alsine, Bothriospermum tenellum, Trigonotis peduncularis and Polygonum arviculare were dominated in the winter cropping on drained paddy field, additionally. In the summer crop field, Digitaria sanguinalis, Portulaca oleracea, Acalypha australis, Echinochloa crus-galli, Setaria viridis, Persicaria hydropiper, Amaranthus lividus, commelina communis, Chenopodium album and Cyperecs amuricus were dominated.

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