• The Journal of the Korea Contents Association
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• v.15 no.12
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• pp.18-23
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• 2015

This paper proposes a new protection technique against deregistration attack in SIP. Although it is caused by simple spoofing the REGISTER message of a legitimate SIP UA, its impact is serious. This new protection technique identifies and protects the deregistration attack by removing a binding form the location server after delaying a certain period of time instead of removing the binding immediately after receiving deregistration message. Therefore, this technique makes it possible to establish a secure SIP environment defending the deregistraion attack without any additional overhead such as an encryption or authentication.

• Journal of Life Science
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• v.5 no.1
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• pp.26-32
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• 1995

The antimutagenic effects of insoluble dietary fibers(IDF) extracted from some green-yellow vegetables(kale, carrot, spinach, broccoli and soybean sprout) and soybean by binding the carcinogens of MeIQ (2-amino-3,4- dimethyl-imidazo(4,5-f) quinoline) and Trp-P-2(3-amino-1-methyl-5H-pyrido[4,3-b] indole) in Salmonella tylhimirium TA100 and TA98 were studied. All of the insoluble dietary fiber samples which binded MeIQ exhibited high antimutagenic effects by removing the mutagen. Among the samples, IDFs from kale and soybean showed strong binding capacity fo the carcinogen and revealed about 90% of the antimutagenic activity. the IDF samples showed somewhat lower binding capacity to the Trp-p-2. The lignin which extracted from kale, soybean and carrot, and the cellulose strongly removed the mutagenicity of MeIQ by the binding. Among the samples, the level of lignin in kale revealed the highest(about 10%), and it seemed that the higher content of lignin in kale is one of the reasons to increase its antimutagenic effect.

• The Korean Journal of Ecology
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• v.26 no.5
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• pp.263-266
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• 2003

This research investigated the process of removing cadmium and tested the detoxification mechanism of the cadmium-binding peptide (Cd-BP) from Rumex crispus. Phytochelatin-like cadmium-binding peptide (PC-Cd-BP) of Rumex crispus was purified and identified. Rumex crispus was exposed to 4.3 mg Cd/L for seven days. Heat-treated supernatant fraction taken by root tissues showed traces of PC-Cd-BP An analysis of the material through Gel-filteration chromatography on the Sephadex G-75 column showed two symmetrical Cd-BP peaks. The major peak with the smaller molecular weight was further purified by $C_{18}$ reverse-phase HPLC to produce apparent homogeneity. The amino acid composition of Cd-BP from Rumex crispus included cysteine (22.6%), glutamate and glutamate acid (20%), and glycine (12%). It was similar the amino acid composition of most PC. The molecular weight of the purified peptide was determined at 568-706 Da by MALDI-TOF MS. Therefore, the Cd-BP of Rumex crispus was PC-Cd-BP consisting of isopeptides.

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1088-1097
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• 2021

Grouper nervous necrosis virus (GNNV) infection causes mass grouper mortality, leading to substantial economic loss in Taiwan. Traditional methods of controlling GNNV infections involve the challenge of controlling disinfectant doses; low doses are ineffective, whereas high doses may cause environmental damage. Identifying potential methods to safely control GNNV infection to prevent viral outbreaks is essential. We engineered a virus-binding bacterium expressing a myxovirus resistance (Mx) protein on its surface for GNNV removal from phosphate-buffered saline (PBS), thus increasing the survival of grouper fin (GF-1) cells. We fused the grouper Mx protein (which recognizes and binds to the coat protein of GNNV) to the C-terminus of outer membrane lipoprotein A (lpp-Mx) and to the N-terminus of a bacterial autotransporter adhesin (Mx-AIDA); these constructs were expressed on the surfaces of Escherichia coli BL21 (BL21/lpp-Mx and BL21/Mx-AIDA). We examined bacterial surface expression capacity and GNNV binding activity through enzyme-linked immunosorbent assay; we also evaluated the GNNV removal efficacy of the bacteria and viral cytotoxicity after bacterial adsorption treatment. Although both constructs were successfully expressed, only BL21/lpp-Mx exhibited GNNV binding activity; BL21/lpp-Mx cells removed GNNV and protected GF-1 cells from GNNV infection more efficiently. Moreover, salinity affected the GNNV removal efficacy of BL21/lpp-Mx. Thus, our GNNV-binding bacterium is an efficient microparticle for removing GNNV from 10‰ brackish water and for preventing GNNV infection in groupers.

• BMB Reports
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• v.34 no.6
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• pp.489-495
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• 2001

DNA damage by UV and environmental agents are the major cause of genomic instability that needs to be repaired, otherwise it give rise to cancer. Accordingly, mammalian cells operate several DNA repair pathways that are not only responsible for identifying various types of DNA damage but also involved in removing DNA damage. In mammals, nucleotide excision repair (NER) machinery is responsible for most, if not all, of the bulky adducts caused by UV and chemical agents. Although most of the proteins involved in NER pathway have been identified, only recently have we begun to gain some insight into the mechanism by which proteins recognize damaged DNA. Binding of Xeroderma pigmentosum group C protein (XPC)-hHR23B complex to damaged DNA is the initial damage recognition step in NER, which leads to the recruitment of XPA and RPA to form a damage recognition complex. Formation of damage recognition complex not only stabilizes low affinity binding of XPA to the damaged DNA, but also induces structural distortion, both of which are likely necessary for the recruitment of TFIIH and two structure-specific endonucleases for dual incision.

• Journal of the Semiconductor & Display Technology
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• v.15 no.4
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• pp.92-96
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• 2016

Although deregistration attack is caused by simple spoofing the REGISTER message of a legitimate SIP UA, its impact is serious. The root cause of this attack is based on the fact that RFC 3261 allows the UA to remove the binding from the Location Server. In this paper, we propose a simple protection method to allow Registrar or Location Server just to ignore deregistration messages. We also show that this method works well by analyzing the process of registration and deregistration. Without any additional overhead such as an encryption or authentication, this method is able to establish a secure SIP environment efficiently protecting against the deregistration attack.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1612-1616
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• 2009

Isoflavonoids are a class of phytoestrogens. Isoflavonone synthase (IFS) is responsible for the conversion of naringenin to genistein. IFS is a cytochrome P450 (CYP), and requires cytochrome P450 reductase (CPR) for its activity. Additionally, the majority of cytochrome P450s harbor a membrane binding domain, making them difficult to express in Escherichia coli. In order to resolve these issues, we constructed an inframe fusion of the IFS from red clover (RCIFS) and CPR from rice (RCPR) after removing the membrane binding domain from RCIFS and RCPR. The resultant fusion gene, RCIFS-RCPR, was expressed in E. coli. The conversion of naringenin into genistein was confirmed using this E. coli transformant. Following the optimization of the medium and cell density for biotransformation, $60\;{\mu}M$ of genistein could be generated from $80\;{\mu}M$ of naringenin. This fusion protein approach may be applicable to the expression of other P450s in E. coli.

• Journal of KIISE:Computer Systems and Theory
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• v.29 no.9
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• pp.493-502
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• 2002

The connectivity and edge-connectivity have been the prime deterministic measure of fault tolerance in multicomputer networks. These parameters have a problem that they do not differentiate the different types of disconnected graphs which result from removing the disconnecting vertices or disconnecting edges. To compensate for this shortcoming, one can utilize generalized measures of connectedness such as superconnectivity, toughness, scattering number, vertex-integrity, binding number, and restricted connectivity. In this paper, we analyze such deterministic measures of fault tolerance in recursive circulants and hypercubes, and compare them in terms of fault tolerance.

• Herbal Formula Science
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• v.24 no.3
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• pp.195-211
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• 2016

Objectives : The purpose of this study was to investigate the interpretation of the Daeseunggi-tang(DST) in order to obtain the evidence for clinical applications.Methods and Results : We have analyzed the interpretation on the DST through translations and comparisons based on classic books about the oriental medical prescriptions.Conclusion : 1. 'Dae(大)' in DST means 'strong symptoms' or 'strong efficacy'. 'Seung(承)' in DST means 'smooth (順)', 'attach(連)' and 'serve(奉)'. 2. The main pathogenesis of DST's syndrome are heat binding(熱結)·qi stagnation(氣滯). 3. The main areas where disease pattern of DST is 'Yang brightness(陽明)'. It is also associated with 'Lesser yin (少陰)', 'Qi aspect(氣分)·Blood aspect(血分)' and 'Triple Energizer(三焦)'. 4. Laxative the Rhei Radix et Rhizoma(大黃) is sovereign medicinal(君藥) of DST, because of removing the heat binding(熱結) and the heat that spread throughout the body.

• Journal of the Korea Institute of Building Construction
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• v.16 no.3
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• pp.219-227
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• 2016

Chloride ions ingress continuously in reinforced concrete through pores of it by $Cl^-$. Finally, it causes a localized corrosion of the rebar and then it generates cracks on concrete structures. Recently, new materials removing harmful anions have been developed. Layered double hydroxides(LDHs) has an excellent ability to remove harmful anions because various anions can be adsorbed in the interlayer space between divalent and trivalent cations. Thus, LDHs has been applied in various fields. Especially, LDHs is expected to be effective adsorbent binding chloride ions. In this study, $Ca/Al-NO_3$ and $Mg/Al-NO_3$ LDHs were prepared by using a co-precipitation method. $Ca/Al-NO_3$ and $Mg/Al-NO_3$ LDHs were compared and analyzed by using XRD, SEM analysis. Many nano size hexagonal crystals were observed by SEM. Experiments for binding chloride ions of LDHs were conducted by using potentiometric method. The experimental data were measured every 15 minutes. It was observed that the chloride ion content is reduced by increasing of LDHs mass fraction and the reaction rate of $Mg/Al-NO_3$ is faster than $Ca/Al-NO_3$. In future studies, binding chloride capacity in cement materials will be evaluated based on results of this study.