• 한국컴퓨터정보학회논문지
• /
• 제14권10호
• /
• pp.207-215
• /
• 2009

최근 도시화 율이 증가됨에 따라 발생되는 도시의 범죄 예방을 위하여 컴퓨터정보기술과 GIS 기술을 이용한 범죄 공간의 분석에 대한 연구가 활발하게 이루어지고 있다. 본 논문에서는 정적인 환경에서 도시공간정보에 포함된 지역특성을 이용하여 단위 셀별 우범 위험도를 계량화하여 래스터 형태의 확률 지도를 구성하였다. 지형적 특성에 의한 상대적 위험도, 시설물에 의한 상대적 위험도, 수목이나 하천에 의한 위험도 등을 기본으로 하여 위험도 확률 지도를 구축한다. 이를 통합한 위험도 확률 지도를 구할 때는 각각의 단위 위험도에 기후나 계절적 요인에 의해 가중치를 적용한 후 평균하게 된다. 또한 일회성 분석이 아닌 범죄 발생 상대적 위험지수의 패턴을 판독키로 하여 전체 위험도의 확률 지도를 생성하여 이후 발생하는 범죄의 유형을 계량화하는 확률지도에 추가적으로 적용하어 정적인 정보가 아닌 시간의 흐름에 따라 범죄 위험도 확률지도가 달라질 수 있는 모델과 시뮬레이션 하는 방법론을 제안하였다.

• Journal of Pharmaceutical Investigation
• /
• 제41권1호
• /
• pp.25-30
• /
• 2011

Recently, Korean Food and Drug Administration (KFDA) has focused on developing quality control guidelines for all commercial products in Korea to enforce regulations, improve the quality control, and protect consumers by developing prevalently used and efficient analytical tools to determine and quantify target compounds. Because the Korean Pharmacopeia (KP) presents microbiological assays for biotin, which is laborious and time-consuming, this study is focused on applying HPLC-UV to detect and quantify biotin in complex drugs and dietary supplements like multi-vitamin. Biotin in complex drugs was extracted from methanol and analyzed using mobile phase with 10 mM potassium phosphate (monobasic, pH=3.0) in distilled water and acetonitrile. Gradient condition was used to successfully detect and quantify biotin within 20 minutes. Validation result for linearity was significant that average $r^2$ was 0.999 (n=3) and its relative standard deviation (RSD) was 0.0578% which was less than 2%. Using this method, quantification of biotin in complex drugs was completed successfully and recovery tests were finished that recovery percentage greater than 95% with relative standard deviation less than 2%.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권5호
• /
• pp.2305-2309
• /
• 2012

Crocin, the main pigment of Crocus sativus L., has been shown to have antiproliferative effects on cancer cells, but the involved mechanisms are only poor understood. This study focused on probable effect of crocin on the immortality of hepatic cancer cells. Cytotoxicity of crocin ($IC_{50}$ 3 mg/ml) in hepatocarcinoma HepG2 cells was determined after 48 h by neutral red uptake assay and MTT test. Immortality was investigated through quantification of relative telomerase activity with a quantitative real-time PCR-based telomerase repeat amplification protocol (qTRAP). Telomerase activity in 0.5 ${\mu}g$ protein extract of HepG2 cells treated with 3 mg/ml crocin was reduced to about 51% as compared to untreated control cells. Two mechanisms of inhibition, i.e. interaction of crocin with telomeric quadruplex sequences and down regulation of hTERT expression, were examined using FRET analysis to measure melting temperature of a synthetic telomeric oligonucleotide in the presence of crocin and quantitative real-time RT-PCR, respectively. No significant changes were observed in the $T_m$ telomeric oligonucleotides, while the relative expression level of the catalytic subunit of telomerase (hTERT) gene showed a 60% decrease as compared to untreated control cells. In conclusion, telomerase activity of HepG2 cells decreases after treatment with crocin, which is probably caused by down-regulation of the expression of the catalytic subunit of the enzyme.

• 대한산업공학회지
• /
• 제30권1호
• /
• pp.44-49
• /
• 2004

The aim of this study is to compare the methods in analyzing bio-signals representing measure driver's psychophysiological staus. This study has considered three approaches: first, the deterministic approach calculating the mean and standard deviation of bio-signal, second, probabilistic approach converting driver's bio-signal values to probability density function and identifying individual state relative to overall distribution, and third, diagnostic approach identifying the pattern change of signal over certain period of time. For evaluation of analysis methods, driver's bio-signal was collected under various road conditions, and three analysis approaches were applied respectively. In result, the deterministic approach was found to be simple to use, but generated a large variability of bio-signal. The probabilistic approach provide a relative status of individual driver among overall population, but too much affected by temporal variability of individual driver. The diagnostic approach seemed to reasonably find driver's psychophysiological change over certain period of time, but still needs to develop quantification method of the bio-signal.

• Bulletin of the Korean Chemical Society
• /
• 제24권11호
• /
• pp.1571-1574
• /
• 2003

This paper describes an inductively coupled plasma emission spectrometric method for the analysis of Fe in rat plasma. Calibration curves were obtained in the range of 0.125-1.50 ${\mu}g{\cdot}mL^{-1}$. The relative standard deviation ranges from 5.93% to 6.80%, and accuracy was between 87.6 and 102.0%. Dilution with water had no influence on the performance of the method, which could then be used to quantify Fe concentration in plasma up to 0.50 ${\mu}g{\cdot}mL^{-1}$. The limit of quantification was 0.10 ${\mu}g{\cdot}mL^{-1}$. At this level, the average relative standard deviation was 6.8%. The results indicate that the method meets the accuracy and precision requirements for the pharmacokinetic studies. The Fe concentration in rat plasma was measured and the main pharmacokinetic parameters were calculated by Topfit 2.0 (GmbH. Shering AG, Godecke AG, Germany).

• 한국축산식품학회지
• /
• 제43권6호
• /
• pp.949-960
• /
• 2023

This research aimed to validate a high-performance liquid chromatography method for the quantitative determination of bixin and norbixin in various foods. The Diode Array Detector (495 nm) technique was used. Method was validated for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. The calibration curve showed excellent linearity (r²≥0.9999) over the tested concentration range of 0.2-25 mg/L. The LOD and LOQ were 0.03-0.11 and 0.02-0.05 mg/L for bixin and norbixin, respectively. The intra-and inter-day accuracies and precisions were 88.0±1.3-97.0±0.5% and 0.2%-2.6% relative SD (RSD) for bixin and 88.2±0.8-105.8±0.8% and 0.3%-2.7% RSD for norbixin, respectively. Inter-laboratory validation for accuracy and precision was conducted in three laboratories, and these results all met the AOAC guidelines. In addition, the relative expanded uncertainty (<22%) satisfied the CODEX recommendation. Furthermore, products distributed in Korea were monitored for annatto extracts using the proposed method to demonstrate its application. The developed analytical method is reliable for quantifying bixin and norbixin in various foods.

• 한국안전학회지
• /
• 제30권4호
• /
• pp.174-180
• /
• 2015

This study developed a Safety Culture Impact Assessment Model (SCIAM) which consists of a safety culture assessment methodology and a safety culture impact quantification methodology. The SCIAM uses safety culture impact index (SCII) to monitor the status of safety culture of the NPPs periodically and it uses relative core damage frequency (RCDF) to present the impact of safety culture on the safety of the NPPs. As a result of applying SCIAM to the reference plant (Kori 3), the standard for the healthy safety culture of the reference plant is suggested. SCIAM might contribute to improve the safety of the NPPs (Nuclear Power Plants) by monitoring the status of safety culture periodically and presenting the standard of healthy safety culture.

• Communications for Statistical Applications and Methods
• /
• 제27권1호
• /
• pp.109-128
• /
• 2020

A climate-impact projection usually consists of several stages, and the uncertainty of the projection is known to be quite large. It is necessary to assess how much each stage contributed to the uncertainty. We call an uncertainty quantification method in which relative contribution of each stage can be evaluated as uncertainty decomposition. We propose a new Bayesian model for uncertainty decomposition in climate change impact assessments. The proposed Bayesian model can incorporate uncertainty of natural variability and utilize data in control period. We provide a simple and efficient Gibbs sampling algorithm using the auxiliary variable technique. We compare the proposed method with other existing uncertainty decomposition methods by analyzing streamflow data for Yongdam Dam basin located at Geum River in South Korea.

• Asian-Australasian Journal of Animal Sciences
• /
• 제11권3호
• /
• pp.209-216
• /
• 1998

The paper presented here is aimed at increasing knowledge on purine metabolism in ruminants and hence the quantification of microbial cells entering the small intestine from urinaη excretion of purine derivatives. Nucleic acid metabolisms of micro-organisms in the rumen, digestion and absorption of nucleic acids entering the intestines, metabolisms of absorbed and endogenous purines involving de novo synthesis of nucleic acids in the ruminants host, and the relationship between absorbed and excreted purines are reviewed. Principal concerns about an amount of purine derivatives excreted in urine in relation to a change in purine-N: total-N ratios in rumen microbes that leave the rumen are discussed. The use of urinary excretion of purine derivatives as an indicator of the amount of microbial biomass leaving the rumen has to be done with some caution since it may be impossible to get a representative sample of microbes entering the intestine and thus yield estimates are relative rather than absolute.

• 통합자연과학논문집
• /
• 제1권3호
• /
• pp.250-253
• /
• 2008

New issues arise as to surface characterization, quantification and interface formation. Surface and interface control of CdSe nanocrystal systems, one of the most studied and useful nanostructures. Semiconductor quantum dots (QDs) have been the subject of much interest for both fundamental reseach and technical applications in recent years, due mainly to their strong size dependent properties and excellent chemical processibility. In this dissertation, the synthesis of CdSe quantum dots were synthesized by pyrolysis of high-temperature organometallic reagents. In order to modify the size and quality of quantum dots, we controlled the growth temperature and the relative amount of precursors to be injected into the coordinating solvent. Moreover, an effective surface passivation of monodisperse nanocrystals was achieved by overcoating them with a higher-band-gap material. Synthesized CdSe quantum dots were studied to evaluate the optical, electronic and structural properties using UV-absorption, and photoluminescence measurement.