• 한국환경보건학회지
• /
• 제33권1호
• /
• pp.21-29
• /
• 2007

Paeonia lactiflora was stepwise extracted with hexane, chloroform, ethyl acetate, butanol and water. Anti-microbial activity of each extract was investigated. Methanol extract of P. lactiflora revealed anti-microbial activity against S. mutans, C. albicans, and S. aureus. Also, hexane fraction revealed anti-bacterial activity against S. mutans and ethyl acetate fraction acted as potent anti-microbial agent on C. albicans and S. aureus. The relative growth ratio(RGR) of hexane fraction of P. lactiflora against S. mutans were determined as 77.8% in concentration of 0.125 mg/ml, 98.46% in 0.25 mg/ml and 100% in 0.5 mg/ml. The ethyl acetate fraction of P. lactiflora revealed RGR against C. albicans as 52.5% in concentration of 0.125 mg/ml, 60.83% in 0.25 mg/ml and 78.33% in 0.5 mg/ml. It indicate that increasing concentration increase RGR. The measured minimal inhibitory concentration(MIC) of hexane fraction on S. mutans KCTC 5316 strain was 0.5 mg/ml and MIC of ethyl acetate fraction on C. albicans KCTC 7270 was 2.0 mg/ml. The experiment of inhibition to growth of KB roll(oral squamous cell carcinoma) result 61.9% in butanol, 76.7% in hexane extract of P. lactiflora. The hexane extract exhibit potent inhibition effect to the growth of KB cell. These results suggest that the hexane extract of Paeonia lactiflora has antimicrobial activity against S. mutans and has preventive effect to dental caries in addition to potent inhibition to KB cell growth.

• 한국산림과학회지
• /
• 제96권2호
• /
• pp.160-169
• /
• 2007

The data used to develop distance-independent individual models for natural mixed forests were collected from 712 remeasured permanent sample plots (25,526 trees) of 10-year periodic from 1990 to 2000 in Baihe Forest Bureau of Changbai Mountains, northeast China. Based on analyzing relationship between diameter increment of individual trees with tree size, competitive status, and site condition, the diameter growth models for individual trees of 15 species growing in mixed-species uneven-aged forest stands, that have simple form, good predicting precision, and easily applicable, were developed using stepwise regression method. The main variables influencing on diameter increment of individual trees were tree size and competition, however, the site conditions were not significantly related with diameter increment. The tree size variables (lnDBH and $DBH^2$) were the most significant and important predictors of diameter growth existing in all 15 growth models. The diameter increment was directly proportional to tree diameter for each species. For the competitive factors in growth model, the relative diameter (RD), canopy closure (P), and the ratio of diameter of subject tree with maximum diameter (DDM) were contributed to the diameter increment at a certain extent. Other measures of stand density, such as basal area of stand (G) and stand density index (SDI), were not significantly influenced on diameter increment. Site factors, such as site index, slope and aspect were not important to diameter increment and excluded in the final models. The total variance explained by the final models of squared diameter increment ($R^2$) for all 15 species ranged from 35% to 72% and these results compared quit closely with those of Wykoff (1990) for mixed conifer stands. Using independent data set, validation measures were evaluated for predicting models of diameter increment developed in this study. The result indicated that the estimated precision was all greater than 94% and the models were suitable to describe diameter increment.

• Journal of Animal Science and Technology
• /
• 제65권5호
• /
• pp.951-970
• /
• 2023

This study utilized Italian ryegrass silage (IRGS) - based total mixed ration (TMR) as feedstuff and evaluated its effects on rumen fermentation, growth performance, blood parameters, and bacterial community in growing Hanwoo heifers. Twenty-seven Hanwoo heifers (body weight [BW], 225.11 ± 10.57 kg) were randomly allocated to three experimental diets. Heifers were fed 1 of 3 treatments as follows: TMR with oat, timothy, and alfalfa hay (CON), TMR with 19% of IRGS (L-IRGS), and TMR with 36% of IRGS (H-IRGS). Feeding high levels of IRGS (H-IRGS) and CON TMR to heifers resulted in a greater molar proportion of propionate in the rumen. The impact of different TMR diets on the BW, average daily gain, dry matter intake, and feed conversion ratio of Hanwoo heifers during the growing period did not differ (p > 0.05). Furthermore, the blood metabolites, total protein, albumin, aspartate aminotransferase, glucose, and total cholesterol of the heifers were not affected by the different TMR diets (p > 0.05). In terms of rumen bacterial community composition, 264 operational taxonomic units (OTUs) were observed across the three TMR diets with 240, 239, and 220 OTUs in CON, L-IRGS, and H-IRGS, respectively. IRGS-based diets increased the relative abundances of genera belonging to phylum Bacteroidetes but decreased the abundances of genus belonging to phylum Firmicutes compared with the control. Data showed that Bacteroidetes was the most dominant phylum, while Prevotella ruminicola was the dominant species across the three TMR groups. The relative abundance of Ruminococcus bromii in the rumen increased in heifers fed with high inclusion of IRGS in the TMR (H-IRGS TMR). The relative abundance of R. bromii in the rumen significantly increased when heifers were fed H-IRGS TMR while P. ruminicola increased in both L-IRGS and H-IRGS TMR groups. Results from the current study demonstrate that the inclusion of IRGS in the TMR is comparable with the TMR containing high-quality forage (CON). Thus, a high level of IRGS can be used as a replacement forage ingredient in TMR feeding and had a beneficial effect of possibly modulating the rumen bacterial community toward mainly propionate-producing microorganisms.

• 한국산림과학회지
• /
• 제100권4호
• /
• pp.609-615
• /
• 2011

본 연구의 목적은 우리나라 특산식물이면서 희귀식물인 산개나리의 유전자원 보존 및 복원을 위한 생육 환경 특성을 구명하기 위한 것으로, 서로 다른 광 조건을 가진 환경에 식재된 산개나리의 생장과 생리적 특성을 조사 분석하였다. 산개나리 시험구내 광량은 전광의 20%, 60%로 구성되었다. 산개나리의 생리적 특성으로 당년지 길이, 잎 크기 및 무게, 광색소 함량 및 잎 내 질소 함량, 광합성 특성을 분석하였다. 광량이 다른 두 시험구에서 측정한 산개나리의 잎 무게는 전광의 60% 광량을 가진 시험구의 잎이 20% 시험구 잎보다 무거웠으며, 잎 무게와 크기의 비율은 전광의 60% 시험구가 20% 시험구보다 1.47배 컸다. 산개나리 잎의 엽록소 a와 b 함량과 카로테노이드 함량 모두 전광의 60% 광량을 가진 시험구에서 높게 나타났으며, 총 엽록소 함량과 카로테노이드 함량의 비는 전광의 20% 시험구가 60% 시험구보다 높았다. 산개나리의 두 시험구에서 측정한 광합성 속도는 전광의 60% 광량을 가진 시험구가 20% 시험구보다 2.5배 이상 높았으며, 기공전도도와 증산 속도도 광합성 속도와 마찬가지로 전광의 60% 시험구가 20% 시험구보다 각각 2.65배와 1.79배 높았다. 그러나 수분이용효율은 전광의 20% 광량을 가진 시험구가 60% 시험구보다 높았다. 산개나리 잎의 질소 함량에 대한 총 엽록소 함량의 비는 20% 시험구가 60% 시험구보다 1.83배 높았으나, 총 엽록소 함량에 대한 순 광합성 량의 비는 60% 시험구가 20% 시험구보다 2.58배 높은 값을 나타냈다. 결론적으로 산개나리의 생장과 생리적 특성에 영향을 주는 가장 큰 요인은 광량이며, 광량이 높은 조건에서 산개나리의 생장과 생리적 특성은 개선될 수 있다고 판단된다.

• 한국산림과학회지
• /
• 제83권2호
• /
• pp.221-228
• /
• 1994

소나무 유령목(幼齡木)의 생장(生長)에 따른 기관별(器官別) 호흡속도(呼吸速度)를 적외선(赤外線) $CO_2$ 가스 분석기(分析機)로 측정(測定)하여, 이 기초자료(基礎資料)를 Thornley의 생장식(生長式) $$R=(\frac{1-Yg}{Yg})\frac{dW}{dt}+mW$$에 적용(適用), 구성호흡(構成呼吸)에 관계되는 물질전환효율(物質轉換效率)(Yg), 유지호흡계수(維持呼吸係數)(m), 그리고 상대생장속도(相對生長速度)(${\mu}$)를 추정(推定)하였다. 물질전환효율(物質轉換效率) Yg는 0.3637g/g dw/yr, 유지호흡계수(維持呼吸係數) m는 0.094g/g dw/yr, 그리고 상대생장속도(相對生長速度) ${\mu}$는 0.90(2년생(年生))에서 0.33(11년생(年生))까지 연령(年齡) 증가(增加)에 따라 현저히 감소(減少)하는 경향을 보였다. 또, 소나무 유령목(幼齡木)의 총광합성량(總光合成量)(Pg)에 대한 총호흡량(總呼吸量)(R)의 비율(比率)(R/Pg)은 0.6~0.7 이었으며, 년간(年間) Pg의 64%가 새로운 물질(物質)을 구성(構成)하는 구성호흡(構成呼吸)으로 소비(消費)되었고, 체중(體重)(건물중(乾物重))의 3.4%가 유지호흡(維持呼吸)으로 소비(消費)되었다.

• 한국연초학회지
• /
• 제7권1호
• /
• pp.15-23
• /
• 1985

Growth response and evapotranspiration of tobacco as to each soil moisture condition treated with different height of ground water table such as 30cm, 100cm, 170cm, and 230cm were investigated. The results obtained were as follows; 1. Soil water potential in the rhizosphere (25cm depth from surface) treated with ground water table (G.W.T) of 230 cm was less than -6.5 bar at 60 days after transplanting. 2. Dry weight of leaves per plant was the highest in the pot treated with 100 cm G.W.T and was the lowest in the pot treated with 230 cm G.W.T.. Leaves/Stem ratio of dry weight was the lowest in the pot treated with 230 cm G.W.T. This would show that extension of leaves as compared with elongation of stem was suppressed. .3. In the roster stress condition of 230 cm G.W.T., extension of leaves was continued until the latter growth stage, Relative growth rate of leaf area per plant of the pot with 30 cm G.W.T. was the highest through all growth period after fixation of G.W.7., but owing to lower density of leaf, the dry weight of leaves per plant was less rather than that of the pot with 170 cm G.W.T. at 64 days after transplanting. 4. The highest evapotranspiration per plant per day was shown at the 45th day after transplanting in the pots with 30cm and 100cm G.W.T. and at 60 days in the pots with 170cm and 230cm G.W.T respectively. 5. In the soil moisture conditions with 30cm and 100cm G.W.T., evapotranspiration per plant per hour was much higher in the daytime that at night as well as in clear day than in rain-cloudy day owing to loner relative humidity , but in the water stress condition with 230 cm G.W.T., that was much lower. 6. Total evapotranspiration per plant during the whole growing period of 64 days was in order of 30cm > 100 cm > 170 cm > 230 cm G.W.T.. Total evapotranspiration for 34 days after fixation of G.W.T was much higher at night than in the daytime in case of water stress conditions i.e 170cm and 230cm G.W.T. but in normal condition of the pots with 30cm and 100cm G.W.T. that was much higher in the daytime than at night. 7. Leaf water potential of the 3rd and 6th leaf from top at 64 days after transplanting was in order of 100cm > 30 cm > 170cm > 230cm G.W.T. The reason why leaf water potential in the pot with 30cm was less than that in the pot with 100cm G.W.T. would be the abnormal turgidity with low density of cell substrates by the excess water absorption in the pot with 30cm G.W.T..

• 생물환경조절학회지
• /
• 제26권2호
• /
• pp.100-107
• /
• 2017

본 실험은 순환식 수경재배에서 배지 재사용이 여름 파프리카의 생육 및 착과에 미치는 영향을 분석하기 위해 수행되었다. 시험구는 30% 배액 재사용을 기준으로 새배지, 1년 재사용 배지, 2년 재사용 배지로 구분하여 4월 18일부터 2016년 11월 31일까지 30주간 시행하였다. 실험결과 생육초기 초장은 2년 재사용 배지가 신규배지보다 길었지만 이후 역전되어 최종 생육초장은 56.58cm 짧았다. 1그룹 개화위치는 재사용 배지가 새배지에 비해 2.92cm 짧았고, 2그룹 개화위치는 0.31cm 길었다. 생육초기의 상대적 절간장은 재사용 배지를 사용할 경우 새배지를 사용하는 것에 비해 생육 후기의 불균형이 심화되었다. 1, 2년 재사용 배지의 생육마디수는 27.4마디로 가장 적었다. 하지만 수확마디수는 시험구간 차이를 보이지 않았기 때문에 2년 재사용 배지의 수확율이 26.8%로 가장 높았다. 비상품과의 비율은 생육이 진전될수록 증가하는 경향을 보였다. 생체중은 새배지가 227.7g, 2년 재사용 배지가 219.2g으로 재사용 배지가 21.2g 적었다. 건물중은 전 생육기간중 4그룹에서 가장 높았는데, 이 시기의 새배지의 건물중은 17.13g, 1년 재사용 배지 18.26g, 2년 재사용 배지 19.28g으로 배지를 재사용 할수록 증가하는 경향을 보였다. 전 생육기간의 평균 수분함량은 배지를 재사용 할수록 적었는데, 1년 재사용 배지가 2년 재사용배지보다 약 20g 더 적었으며, 이러한 경향은 전 생육 기간 동안 꾸준히 나타났다. 특히 1년 재사용배지는 3그룹 이후부터 다른 시험구에 비해 약 60g 이상 적었다. 결론적으로 코이어 배지를 재사용한 파프리카 순환식 수경재배시 중기생육 이후의 세력약화로 인한 비상품과의 발생을 유의하여 관리한다면 배지의 물리 화학성의 변화 및 병원균 감염에 의한 수량감소와 품질저하 등은 우려하지 않아도 될 것으로 판단된다.

• Korean Chemical Engineering Research
• /
• 제53권5호
• /
• pp.609-613
• /
• 2015

The onset of instability induced by impulsive spin-down of the rigid-body flow placed in the gap between two coaxial cylinders is analyzed by using the energy method. In the present stability analysis the growth rate of the kinetic energy of the base state and also that of disturbances are taken into consideration. In the present system the primary flow is a transient, laminar one. But for the Reynolds number equal or larger than a certain one, i.e. $Re{\geq}Re_G$ secondary motion sets in, starting at a certain time. For $Re{\geq}Re_G$ the dimensionless critical time to mark the onset of vortex instabilities, ${\tau}_c$, is here presented as a function of the Reynolds number Re and the radius ratio ${\eta}$. For the wide gap case of small ${\eta}$, the transient instability is possible in the range of $Re_G{\leq}Re{\leq}Re_S$. It is found that the predicted ${\tau}_c$-value is much smaller than experimental detection time of first observable secondary motion. It seems evident that small disturbances initiated at ${\tau}_c$ require some growth period until they are detected experimentally.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVI)
• /
• pp.140-143
• /
• 2005

This study was concerned with the optimization of liquid culture conditions for mycelial growth and polysaccharide production and its physicochemical properties in Cordyceps militaris. The one factor at a time method was adopted to investigate the effects of medium composition, environmental factors and C/N ratio. Among the these varialbles, glucose 80g/L, yeast extract 10g/L, $MgSO_4{\cdot}7H_{2}O\;0.5g/L$, $KH_{2}PO_4\;0.5g/L$ were proved to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, working volume were identified to be $24^{\circ}C$, 7.0 and 100ml, respectively. Under the optimal conditions, the strategies in shake flask culture and 5L jar fermentor led to mycelial growth of 29.43 g/L, 28.88g/L and polysaccharide production of 2.53g/L, 6.38 g/L, respectively. Among the phisicochemical properties, relative concentrations(w/v) of total sugar, uronic acid, protein and hexoseamine were identified to be 74.07%, 1.13%, 0.91%, and 0.46%, respectively. The fraction of neutral and acidic polysaccharide were identified to be 81.9% and 18.1%, respectively.

• 한국결정성장학회지
• /
• 제8권1호
• /
• pp.117-123
• /
• 1998

초크랄스키 실리콘 기판의 뒷면에 형성된 기계적 손상이 미치는 효과에 대하여 고찰하였다. 기계적 손상의 정도는 레이저 여기/극초단파 반사 광전도 감쇠법에 의한 소수반송자 재결합 수명, 광열범위, X-선 단면 측정 및 습식산화/선택적 식각 방법으로 평가하였다. 그 결과, 웨이퍼 뒷면에 가해지는 기계적 손상의 세기가 강할수록 소수반송자 재결합 수명은 짧아지고, 광열 변위의 평균값은 비례적으로 증가하였으며, 손상된 웨이퍼에서 Grade 1의 과잉 광열 변위값을 1로 봤을 때 과잉 광열 변위의 정규화한 상대 정량 비는 Grade 1: Grade 2:Grade 3 = 1:19.6:41이다.