• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1951-1964
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• 2016

1,4-Dioxane-degrading bacterial consortia were enriched from forest soil (FS) and activated sludge (AS) using a defined medium containing 1,4-dioxane as the sole carbon source. These two enrichments cultures appeared to have inducible tetrahydrofuran/dioxane and propane degradation enzymes. According to qPCR results on the 16S rRNA and soluble di-iron monooxygenase genes, the relative abundances of 1,4-dioxane-degrading bacteria to total bacteria in FS and AS were 29.4% and 57.8%, respectively. For FS, the cell growth yields (Y), maximum specific degradation rate ($V_{max}$), and half-saturation concentration ($K_m$) were 0.58 mg-protein/mg-dioxane, $0.037mg-dioxane/mg-protein{\cdot}h$, and 93.9 mg/l, respectively. For AS, Y, $V_{max}$, and $K_m$ were 0.34 mg-protein/mg-dioxane, $0.078mg-dioxane/mg-protein{\cdot}h$, and 181.3 mg/l, respectively. These kinetics data of FS and AS were similar to previously reported values. Based on bacterial community analysis on 16S rRNA gene sequences of the two enrichment cultures, the FS consortium was identified to contain 38.3% of Mycobacterium and 10.6% of Afipia, similar to previously reported literature. Meanwhile, 49.5% of the AS consortium belonged to the candidate division TM7, which has never been reported to be involved in 1,4-dioxane biodegradation. However, recent studies suggested that TM7 bacteria were associated with degradation of non-biodegradable and hazardous materials. Therefore, our results showed that previously unknown 1,4-dioxane-degrading bacteria might play an important role in enriched AS. Although the metabolic capability and ecophysiological significance of the predominant TM7 bacteria in AS enrichment culture remain unclear, our data reveal hidden characteristics of the TM7 phylum and provide a perspective for studying this previously uncultured phylotype.

• The Journal of Advanced Prosthodontics
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• v.7 no.2
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• pp.172-177
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• 2015

PURPOSE. The purpose of this study was to evaluate cell toxicity due to ion release caused by galvanic corrosion as a result of contact between base metal and titanium. MATERIALS AND METHODS. It was hypothesized that Nickel (Ni)-Chromium (Cr) alloys with different compositions possess different corrosion resistances when contacted with titanium abutment, and therefore in this study, specimens ($10{\times}10{\times}1.5mm$) were fabricated using commercial pure titanium and 3 different types of Ni-Cr alloys (T3, Tilite, Bella bond plus) commonly used for metal ceramic restorations. The specimens were divided into 6 groups according to the composition of Ni-Cr alloy and contact with titanium. The experimental groups were in direct contact with titanium and the control groups were not. After the samples were immersed in the culture medium - Dulbecco's modified Eagle's medium[DMEM] for 48 hours, the released metal ions were detected using inductively coupled plasma mass spectrometer (ICP-MS) and analyzed by the Kruskal-Wallis and Mann-Whitney test (P<.05). Mouse L-929 fibroblast cells were used for cell toxicity evaluation. The cell toxicity of specimens was measured by the 3-{4,5-dimethylthiazol-2yl}-2,5-diphenyltetrazolium bromide (MTT) test. Results of MTT assay were statistically analyzed by the two-way ANOVA test (P<.05). Post-hoc multiple comparisons were conducted using Tukey's tests. RESULTS. The amount of metal ions released by galvanic corrosion due to contact between the base metal alloy and titanium was increased in all of the specimens. In the cytotoxicity test, the two-way ANOVA showed a significant effect of the alloy type and galvanic corrosion for cytotoxicity (P<.001). The relative cell growth rate (RGR) was decreased further on the groups in contact with titanium (P<.05). CONCLUSION. The release of metal ions was increased by galvanic corrosion due to contact between base metal and titanium, and it can cause adverse effects on the tissue around the implant by inducing cytotoxicity.

• The Korean Journal of Mycology
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• v.34 no.2
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• pp.112-118
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• 2006

To select efficient entomopathogenic fungal strains of Lecanicillium for the biocontrol of aphid, Myzus persicae, conidial suspension ($1{\times}10\;conidia/ml$) was sprayed onto a detached Chinese cabbage leaf in a petri dish with a dampened filter paper that had 20 nymphs of aphid. Lecancillium strain 4078 and 6543 were the best strains for the biocontrol of aphid at high temperature of $30^{\circ}C$ and low relative humidity (RH) of 85%, respectively. The cumulative mortality of strain 4078 at $30^{\circ}C$ after 3 days was 100% and that of strain 6543 was 90% at 85% RH after 5 days. Strain 4078 also exhibited almost 100% germination ratio of conidia and high rate of mycelial growth at the broad temperature-range of $15{\sim}25^{\circ}C$. The strain 4078 and 6543 were all identified as Lecanicillium species based on the DNA sequences (accession no.: EF026004 and EF026005, respectively) of the ITS regions of the fungi. Excellent production of aerial conidia of strain 6543 was accomplished by using steamed polished rice as the solid culture medium.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.6
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• pp.77-88
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• 2021

Duck industry had a rapid growth in recent years. Nevertheless, researches to improve duck house environment are still not sufficient enough. Moisture generation of duck house litter is an important factor because it may cause severe illness and low productivity. However, the measuring process is difficult because it could be disturbed with animal excrements and other factors. Therefore, it has to be calculated according to the environmental data around the duck house litter. To cut through all these procedures, we built several machine learning regression model forecasting moisture generation of litter by measured environment data (air temperature, relative humidity, wind velocity and water contents). 5 models (Multi Linear Regression, k-Nearest Neighbors, Support Vector Regression, Random Forest and Deep Neural Network). have been selected for regression. By using R-Square, RMSE and MAE as evaluation metrics, the best accurate model was estimated according to the variables for each machine learning model. In addition, to address the small amount of data acquired through lab experiments, bootstrapping method, a technique utilized in statistics, was used. As a result, the most accurate model selected was Random Forest, with parameters of n-estimator 200 by bootstrapping the original data nine times.

• Journal of Korea Port Economic Association
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• v.37 no.1
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• pp.103-119
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• 2021

Ports in Korea have been increasing in terms of volume while they have performed functions and roles such as industrial ports in promoting industries of their hinterlands as well as commercial ports supporting imports and exports. Nevertheless, specialization degree is different from port to port by cargo type and the changes in cargo volume. This study aims to analyze the structural changes and the degree of concentration and specialization by cargo type and port between 2001 and 2020. Top 10 ports were analyzed in terms of traffic volume by categorizing liquid, dry, general cargo and containers. HHI(Herfindahl-Hirschman Index), LQ(Location Coefficient), and shift-share analysis were employed in order to identify the degree of concentration, specialization and changes in cargo volume by port and cargo type. As a result of the analysis, the degree of port concentration and specialization for each cargo of 4 categories have maintained a high level, and no significant difference were found in fluctuations over the past 20 years. As a result of calculating the flucation of cargo volume through the shift-share analysis, the growth rate of liquid cargo was high in Yeosu Gwangyang Port, Pyeongtaek Dangjin Port in dry cargo, and Busan Port in general cargo and container ports. The result implies that it is not expected that the structural changes including degree of cargo concentration, specialization and relative fluctuation of cargo volume is significant in Korean ports in the future since the effects of economies of scale and clustering were achieved to the great degree.

• Animal Bioscience
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• v.35 no.11
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• pp.1733-1743
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• 2022

Objective: This study was aimed to explore the efficacy of combination of endo-xylanase (Xyn) and xylan-debranching enzymes (arabinofuranosidase, Afd and feruloyl esterase, FE) in improving utilization of bran in piglet diet. Methods: In vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd, and FE, concurrent with their effect on degradation of arabinoxylan (Abx) in bran. In vivo experiment was then implemented by allocating two hundred and seventy 35-d-old postweaning piglets into 3 groups (6 replicates/group), which received bran-containing diet supplemented with Xyn (1,600 U/kg) or its combination with Afd (0.8 U/kg) and FE (4 U/kg) or without enzyme. Results: Both Xyn, Afd, and FE are relatively stable against the changes in temperature and pH value. Combining Xyn with Afd and FE had a superiority (p<0.05) over Xyn alone and its combination with Afd or FE in promoting (p<0.05) degradation of Abx in different brans. Combined treatment with Xyn, Afd, and FE was more beneficial than Xyn alone to induce increasing trends (p<0.10) of average daily gain, final body weight and feed efficiency of piglets fed bran-containing diet. Moreover, combination of Xyn, Afd, and FE showed advantages (p<0.05) over Xyn alone in causing reductions (p<0.05) in diarrhea rate and cecal pH value, concurrent with increases (p<0.05) in cecal and colonic acetic acid and total volatile fatty acid concentrations, as well as cecal butyric acid concentration of piglets fed bran-containing diet. Conclusion: Combining Xyn with Afd and FE was more beneficial than Xyn alone in promoting degradation of Abx in bran, along with growth performance and intestinal volatile fatty acid profile of piglets received bran-containing diet. Thereby, combination of Xyn, Afd, and FE had a superior efficacy relative to Xyn alone in improving application of cereal bran in piglet diet.

• Korean Journal of Remote Sensing
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• v.38 no.5_1
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• pp.597-608
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• 2022

Remotely sensed data have been used in various fields, such as disasters, agriculture, urban planning, and the military. Recently, the demand for the multitemporal dataset with the high-spatial-resolution has increased. This manuscript proposed an automatic image matching algorithm using a deep learning technique to utilize a multitemporal remotely sensed dataset. The proposed deep learning model was based on High Resolution Net (HRNet), widely used in image segmentation. In this manuscript, denseblock was added to calculate the correlation map between images effectively and to increase learning efficiency. The training of the proposed model was performed using the multitemporal orthophotos of the National Geographic Information Institute (NGII). In order to evaluate the performance of image matching using a deep learning model, a comparative evaluation was performed. As a result of the experiment, the average horizontal error of the proposed algorithm based on 80% of the image matching rate was 3 pixels. At the same time, that of the Zero Normalized Cross-Correlation (ZNCC) was 25 pixels. In particular, it was confirmed that the proposed method is effective even in mountainous and farmland areas where the image changes according to vegetation growth. Therefore, it is expected that the proposed deep learning algorithm can perform relative image registration and image matching of a multitemporal remote sensed dataset.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.263-269
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• 1997

To cope with increasing importance of water stress in food crop production, some physiological characteristics, their cultivar-differences and grain yield of winter barley cultivars in response to water stress during reproductive stages were studied employing three covered-barley cultivars, Milyang 12, Durubori, and Olbori, one naked-barley cultivar, Baegdong, and one two-row malting-barley cultivar, Hyangmaeg. The barley grown in pot-soil was conditioned for 10 days under water stress, varying the time of water stress : 20 days before heading, 10 days before heading and the time of heading. The decrease in growth due to water stress varied greatly with the cultivars and time of water stress. The greatest injury occurred when water stress was imposed for 10 days from 10 days before heading : the culm length of water-stressed plants have shown reduced by 85∼98％ of the non-stressed; the number of spikes per plant by 52∼83%; the number of grains per spike by 71∼86%; 1,000-grain weight by 80∼84%; yield per pot by 60∼94%. The number of spikes per plant as one of yield components was most sensitively affected. As a whole, the drought resistance of cultivars was high in the order of Olbori> Milyang 12 and Durubori> Hyangmaeg>Baegdong. On rewatering the plants after termination of the water stress treatment the recovery rate of free proline content and relative turgidity of flag leaf were higher in 3 covered-barley cultivars, and lower in cultivars Baegdong and Hyangmaeg.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.3
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• pp.172-181
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• 2023

In order to raise the self-sufficiency rate of domestic forage and obtain informations necessary for production of high-quality winter forage, forage yield and feeding value were analyzed in the above-ground parts of a winter wheat 'Cheongwoo' harvested at five different growth stages, and the appropriate harvest time was determined. The yield increased until 30 days after heading, and then decreased afterwards. The proportion of spike in the above-ground part was less than 40% until 30 days after heading, but increased to more than 60% afterward. At 30 days after heading, the protein content and relative feed value (RFV) also reached peaks, while acid detergent fiber (ADF) and neutral detergent fiber (NDF) were low. The mineral nutrient contents are within the standard range required for feeding ruminant livestock or do not exceed the maximum allowable level. Therefore, the best time to harvest above-ground parts of a winter wheat 'Cheongwoo' for use as a forage for feeding ruminant livestock is around 30 days after heading, when considering forage yield and feed values. As a result it would match well in time with the transplantation of rice seedlings or the sowing of rice-alternative field crops in the double cropping system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.256-267
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• 1987

Changes of light intensity under and above shading materials were measured at different relative light intensity(R.L.I. 5% of common straw shading, 15% of polyester shading and 20% of polyester shading at 12 o'clock on clear day) and lines(lst, 3rd and 5th lines) on clear and cloudy days in 5-year -old ginseng plant populations. Rates of photosynthesis and respiration, microclimate and root yield were also measured in field. Air temperature of R.L.I. 5% were lower 2$^{\circ}C$ compared with R.L.I. 15% and lower 3$^{\circ}C$ compared with R. L. I. 20% from 12 to 14 o'clock on clear day, but there were not difference among R. L. I. on cloudy day. Relative humidity of R. L. I. 5% were higher 5% compared compared with R.L.I. 20% from 10 to 14 o'clock on clear day, R. L. I. on cloudy day. Light intensity were below 5,000 lux at R.L.I. 5%, about 15,000 lux at R.L.I. 15% and about 20,000 lux at R.L.I. 20% from 10 to 15 o'clock on clear day. But light intensity were below 3, 000 lux at R. L. I. 5% about 10, 000 lux at R. L. I. 15% and about 15, 000 lux at R. L. I. 20% from 10 to 15 o'clock on cloudy. Photosynthetic rate of R.L.I. 15% and R.L.I. 20% were higher compared with R.L.I. 5% on clear and cloudy days. Tatal photosynthesis in a day were increased by R.L.I. 5%, 20%, 15% in turn on clear and cloudy days. R. L. I. 15% and 20% were not notable difference of photosynthetic rates among lines but R. L. I. with R. L. I. 15%, and higher 8% but there were not different among 5% was notable difference of one. Root fresh weight were increased by R.L.I. 5%, 20% and 15% in turn and R.L.I. 15% and 20% were not notable difference of root yield among lines but R. L. I. 5% was notable difference of one.