• IMMUNE NETWORK
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• v.1 no.3
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• pp.230-235
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• 2001

Background: Finding of the regulation of various gene expression by cytokine including $IFN-{\gamma}$ in hematopoietic stem cell will light up the understanding of pathogenesis of aplastic anemia in various aspects. To study on aplastic anemia, however, we have to circumvent the difficulty of directly obtaining bone marrow stem cells from the patient. Therefore, we tried to find out a cell can replace the bone marrow stem cells for study on cell signaling pathway and regulation of gene expression by $IFN-{\gamma}$. Materials and Methods: HL-60 cells, of 20 ng/mL of $IFN-{\gamma}$. Total RNA was isolated from the cells and RT-PCR of the indoleamine 2,3-dioxygenase (IDO), $IFN-{\gamma}$, TNF-${\alpha}$, $MIP-1{\alpha}$, and $TGF-{\beta}2$ was carried out for the estimation of the gene expression. Results: $IFN-{\gamma}$ induced IDO gene expression of mononuclear cells from umbilical cord blood showed similar pattern as compared to that of bone marrow. Whether $INF-{\gamma}$ was treated or not, $TNF-{\alpha}$ was expressed in both mononuclear cells from umbilical cord blood and bone marrow. However, HL-60 cells showed different expression patterns. HL-60 cells would express neither IDO nor $TNF-{\alpha}$ even under the culture with 20ng/mL of $IFN-{\gamma}$. Conclusion: Our results showed bone marrow can be replaced with mononuclear cells from umbilical cord blood in the study on the relation between aplastic anemia and $IFN-{\gamma}$ including $IFN-{\gamma}$ cell signaling pathway.

• IMMUNE NETWORK
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• v.3 no.1
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• pp.16-22
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• 2003

Background: The S100A2 gene, also known as S100L or CaN19, encodes a protein comprised of 99-amino acids, is a member of the calcium-binding proteins of EF-hand family. According to a recent study, this gene was over-expressed in several early and malignant carcinomas compared to normal tissues. To elucidate the role of S100A2 protein in the process during carcinogenesis, production of monoclonal antibody specific to the protein is essential. Methods: First, cDNA sequence coding for ORF region of human S100A2 gene was amplified and cloned into an expression vector to produce GST fusion protein. Recombinant S100A2 protein and subsequently, monoclonal antibody to the protein were produced. The specificity of anti-S100A2 monoclonal antibody was confirmed by immunoblot analysis of cross reactivity to other recombinant proteins of S100A family (GST-S100A1, GST-S100A4 and GST-S100A6). To confirm the relation of S100A2 to cervical carcinogenesis, S100A2 protein in early cervical carcinoma tissue was immunostained using the monoclonal antibody. Results: GST-S100A2 recombinant protein was purified by affinity chromatography and then fusion protein was cleaved and S100A2 protein was isolated. The monoclonal antibody (KK0723; Korean patent pending #2001-30294) to the protein was produced and the antibody did not react with other members of EF-hand family proteins such as S100A1, S100A4 and S100A6. Conclusion: These data suggest that anti-S100A2 monoclonal antibody produced in this study can be very useful for the early detection of cervical carcinoma and elucidation of mechanism during the early cervical carcinogenesis.

• International Commerce and Information Review
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• v.1 no.2
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• pp.145-165
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• 1999

With the rapid spreading of the internet that is based on the development of the information network system, the paradigms of "internet round" and "cyber" are given much weight in the notion of "market." Especially, the cyber shopping for the individuals is developing rapidly creating the new life style, and also in the domestic economy the cases of building and running the cyber shopping malls are increasing. The purpose of this study is to analyse the customers' shopping styles that can be shown when the customers purchase physical goods or digital goods at the B to C EC and find the way to activate the shopping malls by controlling the factors which influence the trade. The result of the study is as follows: First, to analyse the acting style of the customers at the B to C EC, it is searched whether there is any relationship between the purchasing goods, which are divided into physical goods and digital goods. There was a cross analysis between the first factor of the five factors of the purchase decision or delay at the B to C EC and the goods type. The result of the analysis is that the purchase decision factor is different according as what type of goods is purchased. On the other hand, the purchase delay factor has no relation with the goods type. Second, the fact that the cyber shopping activities are quite different according to sexuality, age, academic background, or occupation suggests that these factors are very important to the strategy for the market-specification of the B to C EC marketing construction. The result shown in this study is sure to give great help to figure out the improvement strategies and the market-specialization strategies to accelerate the B to C EC marketing. On the side of the strategies for the improvement of the goods services, more attention should be given to the functional side for the improvement of the reliability of the goods service such as capacity, technique, and quality. And the activities of the customers are so different according to the vital statistics that the way to cope with the changeability properly should be considered.

• Journal of Radiation Protection and Research
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• v.22 no.2
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• pp.111-117
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• 1997

Recently, the conclusion of Comprehensive Test Ban Treaty(CTBT) is globally constructing a network system for nuclear test monitoring. The radionuclide experts of the Conference on Disarmament recommended that the detection of nuclear debris in the atmosphere was an essential factor of nuclear test monitoring and proposed the technical requirements. Based on those requirements, atmospheric radionuclide monitoring system to detect nuclear debris generated from the nuclear explosion test was composed. The system is comprised of high volume air sampler(HVAS), filter paper presser and high purity germanium detector(HPGe). Minimum detectable concentrations(MDCs) of the key nuclides requiring in CTBT monitoring strategies are determined by considering of decay time, counting time and flow rate of the high volume air sampler for the rapid explosion and the optimum measurement condition. The results were selected $10{\pm}$2h, $20{\pm}$2h and $850{\pm}50m^3$/h as parameters, respectively. The relation between the natural air-borne radionuclide concentration of $^{212}Pb$ and MDC were calculated which gave effect in the Compton continuum baseline due to those nuclides in the gamma-ray spectroscopy. These results can be used as an actually tool in the CTBT monitoring strategies.

• Journal of The Korean Astronomical Society
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• v.49 no.6
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• pp.261-288
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• 2016

We present the results of simultaneous monitoring observations of $H_2O$ $6_{1,6}-5_{2,3}$ (22GHz) and SiO J=1-0, 2-1, 3-2 maser lines (43, 86, 129GHz) toward five post-AGB (candidate) stars, using the 21-m single-dish telescopes of the Korean VLBI Network. Depending on the target objects, 7 - 11 epochs of data were obtained. We detected both $H_2O$ and SiO maser lines from four sources: OH16.1-0.3, OH38.10-0.13, OH65.5+1.3, and IRAS 19312+1950. We could not detect $H_2O$ maser emission toward OH13.1+5.1 between the late OH/IR and post-AGB stage. The detected $H_2O$ masers show typical double-peaked line profiles. The SiO masers from four sources, except IRAS 19312+1950, show the peaks around the stellar velocity as a single peak, whereas the SiO masers from IRAS 19312+1950 occur above the red peak of the $H_2O$ maser. We analyzed the properties of detected maser lines, and investigated their evolutionary state through comparison with the full widths at zero power. The distribution of observed target sources was also investigated in the IRAS two-color diagram in relation with the evolutionary stage of post-AGB stars. From our analyses, the evolutionary sequence of observed sources is suggested as OH65.5+1.3${\rightarrow}$OH13.1+5.1${\rightarrow}$OH16.1-0.3${\rightarrow}$OH38.10-0.13, except for IRAS 19312+1950. In addition, OH13.1+5.1 from which the $H_2O$ maser has not been detected is suggested to be on the gateway toward the post-AGB stage. With respect to the enigmatic object, IRAS 19312+1950, we could not clearly figure out its nature. To properly explain the unusual phenomena of SiO and $H_2O$ masers, it is essential to establish the relative locations and spatial distributions of two masers using VLBI technique. We also include the $1.2-160{\mu}m$ spectral energy distribution using photometric data from the following surveys: 2MASS, WISE, MSX, IRAS, and AKARI (IRC and FIS). In addition, from the IRAS LRS spectra, we found that the depth of silicate absorption features shows significant variations depending on the evolutionary sequence, associated with the termination of AGB phase mass-loss.

• Journal of The Korean Astronomical Society
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• v.49 no.6
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• pp.295-306
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• 2016

We present the results of BV time-series photometry of the globular cluster NGC 288. Observations were carried out to search for variable stars using the Korea Microlensing Telescope Network (KMTNet) 1.6-m telescopes and a 4k pre-science CCD camera during a test observation from August to December, 2014. We found a new SX Phe star and confirmed twelve previously known variable stars in NGC 288. For the semi-regular variable star V1, we newly determined a period of 37.3 days from light curves spanning 137 days. The light-curve solution of the eclipsing binary V10 indicates that the system is probably a detached system. The pulsation properties of nine SX Phe stars were examined by applying multiple frequency analysis to their light curves. We derived a new Period-Luminosity (P-L) relation, ${\langle}M_V{\rangle}=-2.476({\pm}0.300){\log}P-0.354({\pm}0.385)$, from six SX Phe stars showing the fundamental mode. Additionally, the period ratios of three SX Phe stars that probably have a double-radial mode were investigated; $P_{FO}/P_F=0.779$ for V5, $P_{TO}/P_{FO}=0.685$ for V9, $P_{SO}/P_{FO}=0.811$ for V11. This paper is the first contribution in a series assessing the detections and properties of variable stars in six southern globular clusters with the KMTNet system.

• Journal of Navigation and Port Research
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• v.30 no.1 s.107
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• pp.53-59
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• 2006

Recently, 8,000TEU class containerships has started operating the shipping service and the mega-containership of over 10,000TEU is on planning. A unit cost in relation to shipping service is decreased by the bigger ship based on the economy of scale. Most of the previous studies have been performed and focused on the total operation cost from mega port to mega port. However, the purpose of this paper is to estimate economic efficiency of selected hub ports from point of view of total cost such as service cost(or operation cost), port charge and feeder cost, etc. First, the service-network of mega containerships is based on data of a domestic shipping company operated main line and economic analysis of individual scenarios on the cost and traffic when 10,000TEU mega containerships offer the services. The three scenarios presented in this paper set up the hub ports which are the port of Busan, Shanghai and Yokohama The results show that port of Busan is economically the most efficient one among others.

• The Journal of Fisheries Business Administration
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• v.39 no.1
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• pp.87-114
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• 2008

The cultured tuna production which has suddenly expanded at the short time and the demand for it attract attention. Farming mode, distribution transactions, change of the market (domestic and international) and the price trend are reviewed from the Japan's position which is the biggest consuming country. This paper tries to describe the current status of the food system related to the cultured tuna. Japanese government began the development of the tuna culture technology in 1970. It was by the Fisheries Agency's project. Kinki University which is the large scale private university in Japan participated in the project. After that, 32 years have passed. Kinki University established the full farming of the bluefin tuna in August, 2002. On the other hand, in 1974, one Japanese private enterprise began its tuna farming business in Canada. Kinki University gave this company technical cooperation. Also, in the early stages of the 90s, as for the policy of the overseas fishery cooperation foundation, it supported the tuna farming business in Australia. It is very clear to understand that the long-term technological-development has supported the take-off scene of the tuna culture business not only in foreign countries but also in Japan. The total shipment scale of the cultured tuna expanded very much within about 10 recent years. However, the decrease of the wild tuna catch, the reinforcement of the fisheries regulation and the tuna body to dwarf are remarkable now. Under the condition as the mentioned above, Japan's tuna consumption, especially, in the market at the fatty meat of tuna of the cultured tuna is building up firm status. At present, the Mediterranean Sea coastal countries, Australia, Mexico and Japan have the tuna farming sites. Australia farms the southern bluefin tuna. The others do the bluefin tuna. About for 3 years, Japan farms the juvenile of the tuna. The global production areas are as follows. 8 coastal countries of the Mediterranean Sea; 18,000 tons (61 % of the cultured tuna quantity in foreign countries), Mexico; 4,500 ton (15%), Australia; 7,000 tons (24%). In 2003, Japan has 32 managements and 39 offices for tuna farming. In Japan, Kyushu and Okinawa district, the share shows itself as 80 % of the domestic production quantity. Especially, the share of Amami-oshima Island in Kagoshima Prefecture exceeds 60 %. Therefore, this island has the maximum production scale of Japan. The amount of supply of BT and SBT was 56,000 tons in 2004. In Abroad, the tuna farming business forms a fixed connection between the importer and the wholesaler which have their office in Japan. In the field of the capital composition, the payment in advance, transaction and the way of settlement, each maintains their fixed relation. The market conditions of the cultured tuna are supported by "the decline of price level" and "the expansion of the general public consumption segment". These lead a team merchandising, and it is supported by the fixed business connection of each. This makes the profit of each business which are on the cultured tuna distribution. However, they have competition on the power balance among them.

• Journal of the Korean association of regional geographers
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• v.17 no.2
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• pp.181-203
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• 2011

Transformation towards multicultural society requires discussion on new concepts of citizenship which would overcome some limits of national citizenship developed on the basis of the nation-state. Citizenship can be defined as a relationship between individuals and their community, and conceptualized in a relation with identity. Citizenship also includes its spatial elements such as site and movement, place and public/private space, boundary and territory, flow and network, level and scale, etc. and in particular implies a multi-scalability of local, national, and global level. A new discussion on citizenship has emerged in Japan in shift to multicultural society, especially focusing on activities of local governments and grassroots social movements to support and ensure welfare services to and human rights of foreign immigrants in local communities, hence develops a concept of local citizenship. This concept seems to be highly significant for both foreign immigrants and Japanese dwellers for multicultural coexistence, but raises serious problems of separating local citizenship from formal national citizenship and from universal global citizenship. In order to resolve these problems, a new multiscalar concept of glocal citizenship which links interrelationally local, national and global citizenship. The concept of glocal citizenship is suggested to lead academically a new version of cosmopolitanism which embraces the universal and the particular in a dialectic manner, and to give strategically an alternative to multicultural coexistence policy and discourse and local citizenship discussion in Japan.

• Journal of the Korean association of regional geographers
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• v.20 no.4
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• pp.357-378
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• 2014

The concern on new regional geography in Korea has emerged in the 1990s under the influence of paradigm shift of Western geography, that is, the withering of positivist geography and the introduction of grand social theories into geography. New regional geography in Korea also seems to have developed in the rapidly changing process of glocalization of capitalism which has accompanied with the transformation toward post-Fordism with high-tech innovation, development of transportation and communication technology with time-space compression, and increasing social and cultural mobility with change of identity. But it can be pointed out that discussion on methodology for regional geography in Korea has been shrunken since the mid 2000s, and there has been relatively little empirical research with synthetic approach to region. But more concern on methodology in terms of place, territory, network, scale, etc. rather than the concept of region itself has increased, and empirical researches on regions in specific fields of human geography have been promoted. It is argued that the traditional distinction between synthetic and analytic approaches seems no longer significant. But geographers need to extend the concept of region in relation to other diverse spatial concepts, and to purse simultaneously structural analysis on glocalization process and practical strategies responding positively to the process.