• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05b
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• pp.221-224
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• 2004

By effect of infinity internet market copyright connection skills for extent extension of digital contents and profit rescue through this impoliticly research and develop. Contents protection techniques, management techniques, circulation techniques etc. are becoming development by contents circulation company and developers but that there is no standard as problem advances soul between copyright managerial system by development competition and in reciprocity compatibility tell. ISO MPEG commission arrived that develop MPEG-21 framework to solve problem that do that is to copyright management's opacity province that non efficiency and standard of contents distribution structure that do that is by redundancy investment are not. Data structure in Framework interior is doing and propose copyright expression language of XML base of REL to establish copyright connection data standard and normalized based on no XML. But, a tool that can be easy as must understand whole framework and can have problem in to edit REL and create copyright document fast was required. In this paper, research about method that edit REL document and develop and spend a edit tool that tan be that expressing intuitionally.

• International Journal of Oral Biology
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• v.44 no.2
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• pp.31-36
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• 2019

Streptococcus mutans is one of the important bacteria that forms dental biofilm and cause dental caries. Virulence genes in S. mutans can be classified into the genes involved in bacterial adhesion, extracellular polysaccharide formation, biofilm formation, sugar uptake and metabolism, acid tolerance, and regulation. The genes involved in bacterial adhesion are gbps (gbpA, gbpB, and gbpC) and spaP. The gbp genes encode glucan-binding protein (GBP) A, GBP B, and GBP C. The spaP gene encodes cell surface antigen, SpaP. The genes involved in extracellular polysaccharide formation are gtfs (gtfB, gtfC, and gtfD) and ftf, which encode glycosyltransferase (GTF) B, GTF C, and GTF D and fructosyltransferase, respectively. The genes involved in biofilm formation are smu630, relA, and comDE. The smu630 gene is important for biofilm formation. The relA and comDE genes contribute to quorumsensing and biofilm formation. The genes involved in sugar uptake and metabolism are eno, ldh, and relA. The eno gene encodes bacterial enolase, which catalyzes the formation of phosphoenolpyruvate. The ldh gene encodes lactic acid dehydrogenase. The relA gene contributes to the regulation of the glucose phosphotransferase system. The genes related to acid tolerance are atpD, aguD, brpA, and relA. The atpD gene encodes $F_1F_0$-ATPase, a proton pump that discharges $H^+$ from within the bacterium to the outside. The aguD gene encodes agmatine deiminase system and produces alkali to overcome acid stress. The genes involved in regulation are vicR, brpA, and relA.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1170-1175
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• 2004

The involvement of the relA and rsh genes in the morphological and physiological differentiation of Streptomyces clavuligerus was evaluated with the relA and rsh genes mutants. The morphological differentiation of S. clavuligerus was greatly affected by the disruption of the relA gene, but not very much by the disruption of the rsh gene. The altered morphological characteristics were completely restored by the complementation of the corresponding disrupted genes. Thus, it was apparent that the mycelial morphology and clavulanic acid production were severely affected by the disruption of the relA gene. Production of clavulanic acid in the submerged batch culture and glycerol-limited chemostat showed that production was inversely related to the specific growth rate in the wild-type strain. However, the production of clavulanic acid in the ${\Delta}relA$ and ${\Delta}rsh$ null mutants was completely abolished. Therefore, it seems plausible that the stringent response of S. clavuligerus to starvation for amino acids is governed mainly by ReIA, rather than Rsh, and that the (p)ppGpp synthesized immediately after the depletion of amino acids triggers the initiation of pathways for both morphological and physiological differentiation in this species.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.6
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• pp.353-358
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• 2010

This study demonstrates the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to inhibit LPS-induced expression of iNOS gene and activation of NF-${\kappa}B$/Rel in RAW 264.7 cells. Immunohisto-chemical staining of iNOS and Western blot analysis showed magnolol to inhibit iNOS gene expression. Reporter gene assay and electrophoretic mobility shift assay showed that magnolol inhibited NF-${\kappa}B$/Rel transcriptional activation and DNA binding, respectively. Since p38 is important in the regulation of iNOS gene expression, we investigated the possibility that magnolol to target p38 for its anti-inflammatory effects. A molecular modeling study proposed a binding position for magnolol that targets the ATP binding site of p38 kinase (3GC7). Direct interaction of magnolol and p38 was further confirmed by pull down assay using magnolol conjugated to Sepharose 4B beads. The specific p38 inhibitor SB203580 abrogated the LPS-induced NF-${\kappa}B$/Rel activation, whereas the selective MEK-1 inhibitor PD98059 did not affect the NF-${\kappa}B$/Rel. Collectively, the results of the series of experiments indicate that magnolol inhibits iNOS gene expression by blocking NF-${\kappa}B$/Rel and p38 kinase signaling.

• Communications of the Korean Mathematical Society
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• v.11 no.3
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• pp.815-823
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• 1996

M. Kontsevich posed a problem on mapping class groups of 3-manifold that is if M is a compact 3-manifold with nonempty boundary, then BDiff (M rel $\partial$ M) has the homotopy type of a finite complex. Here, Diff (M rel $\partial$ M) is the group of diffeomorphisms of M which restrict to the identity on $\partial$ M, and BDiff (M rel $\partial$ M) is its classifying space. In this paper we resolve the problem affirmatively in the case when M is a Haken 3-manifold.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.305-312
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• 2007

This study focused on the involvement of the unusual nucleotide (p)ppGpp, a stringent factor, during the morphological and physiological differentiation of Streptomyces coelicolor. Two genes, relA and rshA, were disrupted to demonstrate the roles of the stringent factor in the differentiation. The intracellular concentration of (p)ppGpp in the wild-type (M600) and disrupted mutants was measured in relation to the intentional starvation of a specific nutrient, such as carbon, nitrogen, and phosphate or the in situ depletion of nutrients in a batch culture. As a result, it was found that the morphological characteristic of the ${\Delta}relA$ mutant was a bld phenotype forming condensed mycelia, whereas the ${\Delta}rshA$ mutant grew fast-forming spores and straightforward mycelia. In both mutants, the production of actinorhodin (Act) was completely abolished, yet the undecylprodigiosin (Red) production was increased. Intracellular (p)ppGpp was detected in the ${\Delta}relA$ mutant in the case of limited phosphate, yet not with limited carbon or nitrogen sources. In contrast, (p)ppGpp was produced in the ${\Delta}rshA$ mutant under limited carbon and nitrogen conditions. Therefore, (p)ppGpp in S. coelicolor was found to be selectively regulated by either the RelA or RshA protein, which was differentially expressed in response to the specific nutrient limitation. These results were also supported by the in situ ppGpp production during a batch culture. Furthermore, it is suggested that RelA and RshA are bifunctional proteins that possess the ability to both synthesize and hydrolyze (p)ppGpp.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.8
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• pp.903-910
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• 2016

This paper investigates an advanced receiver for interference mitigation of downlink control channel in the 3GPP Rel-13 standard. There are several features for downlink throughput performance improvement with inter-cell interference management such as network coordination and advanced receivers during Rel-10~Rel-12. These features can be operated always under the assumption that UE perfectly decodes control channels (PCFICH and PDCCH) of serving cell. However, the performance of control channels could be deteriorated in the cell edge region due to inter-cell interference. In this paper, we introduce the advanced receivers and analyze performance for control channel interference mitigation (CCIM) based on 3GPP Rel-13 standard. Additionally, we propose UE behavior depending on network condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.3
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• pp.189-194
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• 1991

This experiment was carried out to investigate the effect of pure mineral nitrogen fertilization on dry matter yield and to determine the amounts of advisable mineral nitrogen according to difference of annual precipitations in permanent grassland. The results obtained were as follows: 1. Only PK-fertilizing without additional mineral nitrogen application produced 6. 9 tonlha of annual dry matter yields on average in relatively wet years that was higher than in rel. dry and normal years under 3-cut system by 0. 7 and 0.6 ton DMIha, respectively. 2. Due to the lesser rainfall the nitrogen-efficiency was substantially higher with heavy dressing of nitrogen fertilizer. The absolute differences in yield between rel. dry and wet years were 0.4 and 0.7 ton DMIhalyear respectively when dressed with 90 and 120 kgN/ha/cut at 4-, 5- and 6-cut systems, whereas at 3-cut system differences around 1.3 and 1.1 ton Dhllhalyear respectively were recorded. 3. In rel. dry years the most efficient N-dressing rates per ha and year tended to be slightly higher than in rel. wet and normal years. Particularly at 5-cut system 4-7 kg/ha/cut of more nitrogen fertilization was required in order to obtain the highest overyields. 4. The N-dressing rates needed to maintain a nitrogen-efficiency of 8 to 16 kg DM/kg N tended to be stronger particularly at high cut system, and also in rel. dry years higher dressing rates were required that in rel. wet and normal years.

• Journal of the Chungcheong Mathematical Society
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• v.6 no.1
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• pp.113-130
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• 1993

In this paper, we introduce certain subgroups $E^{Rel}_n$ (X, Y, K, G) of the relative homotopy groups ${\sigma}_n$(X, Y, K, G) of a transformation group and examine the algebraic properties of these groups.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.8-8
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• 2019

The stringent response (SR) is characterized as a bacterial defense mechanism in response to various growth-inhibiting stresses. It is activated by accumulation of a small nucleotide regulator, (p)ppGpp, and induces global changes in bacterial transcription and translation. Recent work from our group has shown that (p)ppGpp plays a critical role in virulence and survival in Vibrio cholerae. The genes, relA and relV, are involved in the production of (p)ppGpp, while the spoT gene encodes an enzyme that hydrolyzes it in V. cholerae. A mutant strain defective in (p)ppGpp production (i.e. ${\Delta}relA{\Delta}relV{\Delta}spoT$ mutant) lost the ability to produce cholera toxin (CT) and lost their viability due to uncontrolled production of organic acids, when grown with extra glucose. In contrast, the ${\Delta}relA{\Delta}spoT$ mutant, a (p)ppGpp overproducer strain, produced enhanced level of CT and exhibited better growth in glucose supplemented media via glucose metabolic switch from organic fermentation to acetoin, a neutral fermentation end product, fermentation. These findings indicates that (p)ppGpp, in addition to its well-known role as a SR mediator, positively regulates CT production and maintenance of growth fitness in V. cholerae. This implicates SR as a promising drug target, inhibition of which may possibly downregulate V. cholerae virulence and survival fitness. Therefore, we screened a chemical library and identified a compound that induces medium acidification (termed iMAC) and thereby loss of wild type V. cholerae viability under glucose-rich conditions. Further, we present a potential mechanism by which the compound inhibits (p)ppGpp accumulation. Together, these results indicate that iMAC treatment causes V. cholerae cells to produce significantly less (p)ppGpp, an important regulator of the bacterial virulence and survival response, and further suggesting that it has a therapeutic potential to be developed as a novel antibacterial agent against cholera.