• 한국수정란이식학회지
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• 제30권1호
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• pp.23-31
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• 2015

Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and $400{\mu}M$, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol ($100{\mu}M$) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol ($100{\mu}M$) treated group when compared with the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol ($100{\mu}M$) treated group were significantly decreased in the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol ($100{\mu}M$) treated group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect.

• Natural Product Sciences
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• 제13권4호
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• pp.311-316
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• 2007

In this study, the twenty-four ethyl acetate extracts of twenty-two medicinal plants, traditionally used in Vietnam as anti-diabetes agents, were investigated for ${\alpha}$-amylase and protein tyrosine phosphatase 1B (PTP1B) enzymes inhibitory activity in vitro. The results indicated that, twelve materials (50.0%) showed moderate to strong inhibitory activity in ${\alpha}$-amylase inhibitory activity with $IC_{50}$ values ranging from 2.5 to $48.8{\mu}g/mL$; meanwhile, ten extracts (41.6%) could demonstrate PTP1B activity with $IC_{50}$ values less than $30.5{\mu}g/mL$. Some plants presented interesting activities against both of ${\alpha}$-amylase and PTP1B enzymes such as Catharanthus roseus, Carthamus tinctorius, Momordica charantia, Gynostemma pentaphyllum, Glycyrrhiza glabra, Smilax glabra, Psidium guajava (leave), and Rehmannia glutinosa. The study may provide a proof, at least in a part, for the ethno-medical use in diabetes disease of these plants.

• 대한한방소아과학회지
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• 제31권1호
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• pp.63-73
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• 2017

Objectives The purpose of this study is to analyze recent clinical studies on treatment for precocious puberty. Methods 28 clinical studies from January 2009 to December 2016 about precocious puberty from the China Academic Journal (CAJ), China National Knowledge Infrastructure (CNKI), were selected and reviewed: 22 case control studies and 6 case series. Results The main traditional Chinese medicine (TCM) treatment was the herbal decoctions and granules. The frequently used herbs were root of Bupleurum falcatum (柴胡), fruit of Prunella vulgaris var. aleutica (夏枯草), root stem of Anemarrhena asphodeloides (知母), dried fungus nucleus of Poria cocos (茯苓), Rehmannia glutinosa var. purpurea (生地黃), peony Paeonia suffruticosa Andrews (牧丹皮), bark of Phellodendron amurense (黃柏), roots of a plant Paeoniae lactiflora (白芍藥). On the other hand, the main western medicine (WM) for precocious puberty was Gonadotropin-releasing hormone agonist (GnRHa). Total effective rate of the TCM group was 62.96-100%, that of the WM group was 36.6-93.3% and that of the TCM-WM group was 77.8-93.75%. Conclusions Traditional Chinese medicine has been shown as an effective treatment for precocious puberty. These research results can be utilized in other clinical studies and in treatment of precocious puberty.

• 한국수산과학회지
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• 제54권4호
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• pp.411-417
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• 2021

By-products produced from medicinal plants (MP) were evaluated as feed additives on growth performance, blood chemistry, and immune responses of the juvenile red lip mullet Liza haematocheila. A commercial diet was used as the control. Four experimental diets were prepared by combining 0.5% of MPs such as Panax ginseng (PG), Schisandra chinensis (SC), Angelica gigas (AG), and Rehmannia glutinosa (RG), via water absorption, to the control diet. Triplicate groups of fish averaging 61.8 g were fed one of experimental diets to apparent satiation for 8 weeks. Dietary supplementation of MPs did not affect the growth performance, biological indices and whole body composition of fish compared to the control. The fish fed with AG diet had an increased weight gain, and specific growth rate compared to fish in SC group. Decreased level of plasma glucose was observed in fish fed diet containing MPs. Lysozyme activity was significantly increased in the fish fed AG diet compared to that of fish in PG group. These results indicate that dietary supplementation of Angelica gigas may enhance growth performance and immune responses in juvenile red lip mullet.

• 농업과학연구
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• 제46권3호
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• pp.427-437
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• 2019

Oxidative stress and overproduction of free radicals have been reported to be a major pathological hallmark of neurodegenerative diseases. Samultang has been known as a beneficial agent to treat liver disease and cardiovascular diseases. However, the anti-oxidant activities and neuro-protective effects of Samultang against oxidative stress still have not been evaluated yet. The aim of the present study was to investigate the anti-oxidant and protective effects of Samultang and its four herbal plants, Paeonia lactiflora (PL), Ligusticum striatum (LS), Rehmannia glutinosa (RG), and Angelica gigas (AG), in vitro system and in SH-SY5Y neuronal cells. The extracts of Samultang strongly increased the radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (${\cdot}OH$), and nitric oxide (NO) in a concentration-dependent manner. Furthermore, we investigated the protective effects of Samultang on cellular damage against oxidative stress induced by hydrogen peroxide ($H_2O_2$) in SH-SY5Y cells. Treatment with Samultang alleviated the oxidative stress from $H_2O_2$ by increasing the cell viability and decreasing the intracellular reactive oxygen species levels. Based on these results, we further investigated the radical scavenging effects of PL, LS, RG, and AG. In our results, PL had the highest DPPH, ${\cdot}OH$, and NO radical scavenging activities. Thus, PL has a crucial role in Samultang, which has anti-oxidative and neuro-protective effects. The present research suggests that Samultang and PL have protective roles against oxidative stress from $H_2O_2$-induced neuronal cell death.

• Natural Product Sciences
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• 제25권2호
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• pp.103-110
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• 2019

We investigated the anti-inflammatory effect of Pyunkang-tang extract (PGT), a complex herbal extract based on traditional Chinese medicine that is used in Korea for controlling diverse pulmonary diseases, on cigarette smoke-induced pulmonary pathology in a rat model of chronic obstructive pulmonary disease (COPD). The constituents of PGT were Lonicerae japonica, Liriope platyphylla, Adenophora triphilla, Xantium strumarinum, Selaginella tamariscina and Rehmannia glutinosa. Rats were exposed by inhalation to a mixture of cigarette smoke extract (CSE) and sulfur dioxide for three weeks to induce COPD-like pulmonary inflammation. PGT was administered orally to rats and pathological changes to the pulmonary system were examined in each group of animals through measurement of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) levels in bronchoalveolar lavage fluid (BALF) at 21 days post-CSE treatment. The effect of PGT on the hypersecretion of pulmonary mucin in rats was assessed by quantification of the amount of mucus secreted and by examining histopathologic changes in tracheal epithelium. Confluent NCI-H292 cells were pretreated with PGT for 30 min and then stimulated with CSE plus PMA (phorbol 12-myristate 13-acetate), for 24 h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. The results were as follows: (1) PGT inhibited CSE-induced pulmonary inflammation as shown by decreased TNF-${\alpha}$ and IL-6 levels in BALF; (2) PGT inhibited the hypersecretion of pulmonary mucin and normalized the increased amount of mucosubstances in goblet cells of the CSE-induced COPD rat model; (3) PGT inhibited CSE-induced MUC5AC mucin production and gene expression in vitro in NCI-H292 cells, a human airway epithelial cell line. These results suggest that PGT might regulate the inflammatory aspects of COPD in a rat model.

• 대한본초학회지
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• 제24권4호
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• pp.101-106
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• 2009

Objectives : We investigated the anti-aging effects on skin with 9 Repetitive steaming and fermenting herbal composition extract (FHE). Methods : Herbal composition is comprised of Panax Ginseng, Rehmannia glutinosa, Polygonum multiflorum and Polygonatum Sibiricum. They steamed and fermented with Saccharomyces cerevisiae and Lactobacillus brevis by turns at 9 times. We measured various effects related to skin aging such as scavenging activity against free radical, cell proliferation, collagen synthesis and toxicity of 9 repetitive steaming and fermenting herbal composition extract were evaluated and compared with herbal composition extract (HE). Results : As the number of steaming and fermenting cycle increased free-radical scavenging activity were increased. But cell proliferating ratio was not increased when the number of steaming and fermenting cycle. The FHE could significantly increase the collagen synthetic ratio compared with HE treated group. And, FHE was showed no toxicity at all tested concentrations. Conclusions : The results of our study propose that FHE has good anti-aging effects on skin.

• 한국약용작물학회지
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• 제1권1호
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• pp.70-73
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• 1993

지황(地黃)의 재식밀도(栽植密度)가 수양(收量)에 미치는 영화(影響)을 규명(究明)코자 지방재내종(地方在來種)을 공시(共試)하여 두둑 100cm에 휴폭(畦幅) $30cm\;{\times}\;주간(株間)$ l0cm 2열(列)$(20주(株)\;/\;m^2)$, $30\;{\times}\;l0cm\;3열(列)(30주(株)\;/\;m^2),\;30\;{\times}\;l0cm\;4열(列)(40주(株)\;/\;m^2)$로 하고, $N-P_{2}O_{5}-K_{2}O=12-12-l6kg\;/\;10a$, 퇴비 1,000kg/10a를 시용(施用), 4월(月) 11일(日)에 파종(播種)하여 시험(試驗)을 실시(實施)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 출아(出芽)은 재식밀도간(裁植密度間)에 차리(差異)가 없었고, 초장(草長)은 $m^2$당(當) 20주구(株區) 15.5cm에 비(比)하여 30주구(株區)는 0.5cm, 40(주구株區)는 0.7cm가 각각(各各) 짧았으며, 엽장(葉長) 및 엽폭(葉幅)은 처리간(處理間)에 차리(差理)가 없었다. 2. 근장(根長)은 24cm 정도(程度)로 처리간(處理間)에 차리(差理)가 없었으나 근경(根莖)의 굵기는 $m^2$당(當) 20주구(朱區)의 11.4mm에 비(比)하여 밀식(密植)일 수록 0.6mm, 1.2mm가 각각(各各) 가늘었으며, 주당근경수(株當根莖數)는 감소되는 경향(傾向)이었다. 3. 10a당(當) 총(總) 수양(收量)은 $m^2$당(當) 20주구(株區) 793kg에 비(比)하여, 30주구(株區)는 40%, 40주구(株區)는 45%가 각각(各各) 증수(增收) 되었고, 상품수양(商品收量)은 $m^2$당(當) 20주구(朱區)의 420kg에 비(比)하여, 30주구(株區)는 48%, 40주구(株區)는 12%가 각각(各各) 증수(增收)되었으나, 중북부(中北部) 내륙지방(內陸地方)에서 소득(所得)을 고려(考慮)한 지황(地黃)의 적정재식밀도(適正裁植密度)는 $m^2$당(當) 30주(株)로 재배(栽培)하는 것이 유리(有利)하다고 판단(判斷)되었다.

• 한국토양비료학회지
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• 제22권1호
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• pp.45-52
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• 1989

한방(韓方)의 주요(主要)한 약용식물(藥用植物)로 사용(使用)되어 온 지황(地黃)의 품질(品質)을 향상(向上)시키기 위한 합리적(合理的)인 포장선정(圃場選定) 및 시비량(施肥量)을 결정(決定)하기 위하여 산지토양의 이화학성(理化學性) 및 삼요소(三要素) 시용량(施用量)이 지황(地黃)의 무기성분함량(無機成分含量)에 미치는 영향(影響)을 조사(調査)하였다. 재배지토양(栽培地土壤)의 토성(土性)은 사질양토(砂質壤土)~사질식양토(砂質埴壤土)가 대부분(大部分) 이었다. 지황근경(地黃根莖)의 질소함량(窒素含量)은 표토(表土)의 유기물(有機物) 및 전질소함량(全窒素含量), 심토(心土)의 점토함량(粘土含量)과 부(負)의 상관(相關)이, 심토(心土)의 모래함량(含量)과는 고도(高度)의 정(正)의 상관(相關)이 인정(認定)되었다. 지황근경(地黃根莖)의 Ca함량(含量)은 표토(表土) 및 심토(心土)의 모래함량(含量)과 고도(高度)의 부(負)의 상관(相關)이, 표토(表土) 및 심토(心土)의 점토(粘土) 및 유기물함량(有機物含量)과는 정(正)의 상관(相關)이 인정(認定)되었다. 지황근경(地黃根莖)의 Fe 함량(含量)은 표토(表土)의 점토(粘土), 인산(燐酸) 및 가리함량(加里含量)과 정(正)의 상관(相關)이 인정(認定)되었다. 지황엽중(地黃葉中)의 무기성분함량(無機成分含量)은 근경중(根莖中)의 무기성분함량(無機成分含量)보다 많았으며, 특(特)히 Fe와 Cu의 함량(含量)은 약(約) 10배에 달하였다. 근경중(根莖中)의 질소함량(窒素含量)은 20kg/10a, 엽중(葉中)의 질소함량(窒素含量)은 10kg/10a의 질소시용량(窒素施用量)부터 증가(增加) 하였다. 인산(燐酸) 및 가리(加里)의 증시(增施)에 따른 지황근경(地黃根莖) 및 엽중(葉中)의 인(燐) 및 가리(加里)의 함량(含量)에는 일정(一定)한 경향(傾向)이 없었다.

• 한국가금학회지
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• 제34권4호
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• pp.245-251
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• 2007

한방생약제제(Herb $Mix^{(R)}$) 첨가가 중대추의 증체와 산란 개시 및 peak 도달일령, 산란 생산성, 계란 품질, 분내 미생물 균총 및 혈액성상에 대해 미치는 영향을 검정하기 위해 사양시험을 실시하였다. 14주령된 산란계(Hy-Line Brown) 중추 1,120수를 무첨가 대조구(T1), Herb $Mix^{(R)}$(HM)(T2), 지황 강화 HM구(T3), 당귀 강화 HM구(T4), 천궁 강화 HM구(T5), 작약 강화 HM구(T6) 그리고 플라보마이신 구(T7) 등 총 7개의 처리구에 처리당 8반복, 반복당 10케이지, 케이지당 2수씩 수용하여 난괴법으로 배치하였다. 산란율 10% 도달 시 체중은 처리간에 유의한 차이가 있었는데, 당귀 강화 HM구(T4)가 가장 무거웠고 다음으로 천궁 강화 HM구(T5), HM-original구(T2)와 작약 강화 HM구(T6), Flavomycin 구(T7) 그리고 지황 강화 HM구 (T3)이었으며, 대조구(T1)가 가장 가벼웠다. 산란율 50% 도달 일령 및 산란 피크 도달 일령 역시 당귀 강화 HM구(T4)가 가장 빨랐으며, 대조구(T1)가 가장 늦었다. 일계산란율, 난중, 사료 섭취량 및 사료 전환율은 처리간에 유의한 차이가 있었다. 일계산란율에 있어서 당귀 강화 HM구(T4)가 가장 높았고, 전체적으로 HM 첨가구들이 항생제 첨가구(T7)나 대조구(T1)보다 높았다. 난중은 천궁 강화 HM구(T5)가 가장 무거웠으며, 대조구(T1)가 가장 가벼웠다. 사료 전환율에 있어서는 항생제 첨가구(T7)이 2.14로 가장 낮았으며, 당귀 강화 HM구(T4)와 작약 강화 HM구(T6)가 2.30으로 가장 높았다. 모든 장내 미생물들의 수는 처리간에 유의한 차이가 없었지만, Cl. perfringens와 E. coli 수는 모든 첨가구들이 대조구에 비해 낮은 경향을 나타내었다. 백혈구 (WBC) 수와 heterophils(HE)의 수는 처리간에 유의한 차이가 있었는데, WBC는 모든 첨가구 특히 HM 첨가구들이 항생제 첨가구나 대조구에 비해 낮은 수치를 나타내었다. 림프구 (LY)의 경우 유의적 차이는 없었지만, 모든 첨가구가 증가한 경향을 보였다. 반면, stress indicator로 알려진 HE/LY는 HM첨가구들이 항생제 첨가구나 대조구에 비해 낮은 수치를 나타내었다. 결론적으로 중대추의 증체와 산란개시일령 및 peak 도달일령 단축, 산란 생산성에 미치는 Herb $Mix^{(R)}$ 의 첨가 효과는 기존 Herb $Mix^{(R)}$ 처방에 당귀, 작약 및 천궁을 강화시킴으로 개선 효과를 증대시킬 수 있으며, 이러한 결과는 이들 한방제제가 사료섭취량, 혈액 성상 및 장내 미생물에 미치는 영향에 기인하는 것으로 사료된다.