• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.136-139
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• 2009

Avermectin and its analogs are major commercial antiparasitic agents in the fields of animal health, agriculture, and human infections. Previously, comparative transcriptome analysis between the low-producer S. avermitilis ATCC31267 and the high-producer S. avermitilis ATCC31780 using a S. avermitilis whole genome chip revealed that 50 genes were overexpressed at least two-fold higher in S. avermitilis ATCC31780. To verify the biological significance of some of the transcriptomics-guided targets, five putative regulatory genes were individually cloned under the strong-and-constitutive promoter of the Streptomyces expression vector pSE34, followed by the transformation into the low-producer S. avermitilis ATCC31267. Among the putative genes tested, three regulatory genes including SAV213, SAV3818, and SAV4023 exhibited stimulatory effects on avermectin production in S. avermitilis ATCC31267. Moreover, overexpression of SAV3818 also stimulated actinorhodin production in both S. coelicolor M145 and S. lividans TK21, implying that the SAV3818, a putative TetR-family transcriptional regulator, could be a global upregulator acting in antibiotic production in Streptomyces species.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.169-175
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• 2000

The filamentous soil bacterium Streptomyces coelicolor is known to produce four distinct antibiotics. The simultaneous global regulation for the biosynthesis of those four antibiotics was previously confirmed by absA and absB mutations that blocked all four antibiotics' biosynthesis without influencing their morphological differentiation. To study the complex regulatory cascade that controls the secondary metabolism in Streptomyces, a new abs-like mutation was characterized. namely absR, which is slightly leaky on a complete R2YE medium, yet tight on a minimal medium. A genetic analysis of the absR locus indicated that it is located at 10 o'clock on the genetic map, near the site of absA. A cloned copy of the absA gene that encoded bacterial two-component regulatory kinases did not restore antibiotic biosyntheis to the absR mutant. Accordingly, it is proposed that absR is another abs-type mutation which is less tight than the previously identified absA or absB mutations income medium conditions, and can be used to characterize another global regulatory gene for secondary metabolete formation in S. coelicolor.

• IMMUNE NETWORK
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• 제14권2호
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• pp.67-72
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• 2014

The herpes virus entry mediator (HVEM) is a member of the tumor necrosis factor receptor superfamily (TNFRSF), and therefore it is also known as TNFRSF14 or CD270 (1,2). In recent years, we have focused on understanding HVEM function in the mucosa of the intestine, particularly on the role of HVEM in colitis pathogenesis, host defense and regulation of the microbiota (2-4). HVEM is an unusual TNF receptor because of its high expression levels in the gut epithelium, its capacity to bind ligands that are not members of the TNF super family, including immunoglobulin (Ig) superfamily members BTLA and CD160, and its bi-directional functionality, acting as a signaling receptor or as a ligand for the receptor BTLA. Clinically, Hvem recently was reported as an inflammatory bowel disease (IBD) risk gene as a result of genome wide association studies (5,6). This suggests HVEM could have a regulatory role influencing the regulation of epithelial barrier, host defense and the microbiota. Consistent with this, using mouse models, we have revealed how HVEM is involved in colitis pathogenesis, mucosal host defense and epithelial immunity (3,7). Although further studies are needed, our results provide the fundamental basis for understanding why Hvem is an IBD risk gene, and they confirm that HVEM is a mucosal gatekeeper with multiple regulatory functions in the mucosa.

• 한국정보시스템학회지:정보시스템연구
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• 제26권4호
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• pp.303-325
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• 2017

Purpose The purpose of this study is to forage for the factors for explaining the adoption behavior of the mobile convenient payment system based on the Personality Trait, Unified Theory of Acceptance and Use of Technology, and Regulatory focus. Design/methodology/approach This study made a design of the research model by integrating the factors of Unified Theory of Acceptance and Use of Technology with the factors of Big five personality traits on the basis of Samrt device environment. In addition, this study, did empirical analysis of the structural equation model by PLS(Partial Least Squares) in order to find out the relationship of personal factors and mobile convenient payment system's features. Findings According to the empirical analysis result, this study confirmed that the acceptance of mobile convenient payment systems is influence by personality traits and factors based on Unified Theory of Acceptance and Use of Technology and trust. In addition, this study find out that the behavior of an adoption of mobile convenient payment systems according to the type of regulatory focus.

• 한국의류산업학회지
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• 제16권4호
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• pp.580-587
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• 2014

This study demonstrates how the composition of VM based on regulatory focus influences shopping value and how shopping value influences shopping satisfaction. A survey form was developed and 90 copies were used for analysis. SPSS 19.0 for Windows Package was used to perform frequency analysis, factor analysis, and regression analysis. The results of the study were: First, convenience of VM had a significantly positive (+) influence on utilitarian shopping value. Second, both utilitarian shopping value and hedonic shopping value had a statistically significant influence on shopping satisfaction. Third, in terms of promotion focus, suitability, fashionableness, reconcilability, and attractiveness of VM had a significantly positive (+) influence on the hedonic shopping value. In terms of preventive focus, convenience and reconcilability of VM had a significantly positive (+) influence on utilitarian shopping value, and convenience, suitability, and fashionableness had a significantly positive (+) influence on hedonic shopping value. In the promotion focus, both utilitarian shopping value and the hedonic shopping value had a significantly positive (+) influence on shopping satisfaction. In the preventive focus only the utilitarian shopping value had a significantly positive (+) influence on shopping satisfaction.

• 대한의생명과학회지
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• 제20권3호
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• pp.147-155
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• 2014

It has well studied that immune cells are strongly related to tumor progression and tumor suppression. To identify the difference of immune cell between tumor bearing mice and normal mice, we examined systemically the immune cell of CT26 tumor bearing mice on 21 days after tumor cell administration. As previously reported, CD4+ and CD8+ T cells population of tumor bearing mice significantly decreased 38% and 30% on day 21 compared to that of normal mice, respectively. All subpopulation of CD4 and CD8+ T cell significantly decreased, except CD49b+ T cell subpopulation. But, myeloid cell population ($CD11b^{high}$ and all Gr-1+ subpopulation) of tumor bearing mice significantly increased on day 21. Especially, all subpopulation of CD11b+Gr-1+ cell of tumor bearing mice significantly increased on day 21. Also, Foxp3+$CD25^{high}$ CD4 T cell (regulatory T cells) population significantly increased on day 21. These results suggest that tumor can induce the decline of T lymphocyte and the expansion of myeloid cells and regulatory T cells, and provide the basic information for the study of tumor immunology.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3677-3683
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• 2015

Abnormally expressed microRNAs (miRNAs) and genes have been found to play key roles in esophageal squamous cell carcinoma (ESCC), but little is known about the underlying mechanisms. The aim of this paper was to assess inter-relationships and the regulatory mechanisms of ESCC through a network-based approach. We built three regulatory networks: an abnormally expressed network, a related network and a global network. Unlike previous examples, containing information only on genes or miRNAs, the prime focus was on relationships. It is worth noting that abnormally expressed network emerged as a fault map of ESCC. Theoretically, ESCC might be treated and prevented by correcting the included errors. In addition, the predicted transcription factors (TFs) obtained by the P-match method also warrant further study. Our results may further guide gene therapy researchers in the study of ESCC.

• 환경정책연구
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• 제12권3호
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• pp.49-71
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• 2013

독성, 환경 노출 및 인체에 대한 위해성 유해성 등 안전성에 대한 문제점을 가지고 있는 나노물질의 확인 및 안전성 확보의 요구가 점차 높아지고 있다. 나노물질은 화학물질로 이용될 뿐만 아니라 소비자가 이용하는 제품으로 범 분야에 걸쳐 활용되기 때문에, 보다 효과적인 나노물질의 안전관리 제도화를 위해서는 각국의 정책사례를 규제 강도별, 나노 적용분야별, 규제대상 종류별로 구분하여 정책 동향을 파악하는 것이 중요하다. 나노물질의 잠재적 위험성을 최소화시키기 위해서는 사전예방원칙을 적용한 제도의 구축 또한 필요하다. 더불어 국내 실정에 적합한 정책 수립을 목표로 하고, 국제 흐름에 선제적, 능동적으로 대응할 수 있는 방안도 함께 고려해야 한다.

• 한국안전학회지
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• 제15권4호
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• pp.112-118
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• 2000

This paper is resulted from the research to activate Self-control Safety Management system that adopted to improve an assessment system for Hazard Prevention Plan. And members of company, university and research institute have jointly participated in the research. First, it is investigated that introduced background and processing method with existing practical data & materials references in order to understand what domestic Self-control Safety Management system will be available for. And general construction company at site have their ability to assess Hazard Prevention Plan by mailing questionnaire to on thousand site, visits, and interviews. Also, It is investigated how much they have the assessment ability. It is selected to do a questionnaire survey for the status of self-regulatory safety assessment ability of the designated self-regulatory companies in order to produce and enhance the self-regulatory assessment ability and the necessity of Self-control Safety Management system. Finally, it is selected to do a questionnaire survey for fixing and the enhance Self-control Safety Management system of general construction contractors.

• 대한기계학회논문집A
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• 제34권4호
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• pp.495-499
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• 2010

이 연구에서는 우리나라 CANDU형 원전의 계속운전 규제지침을 개발하였다. CANDU 600 로형인 월성 1호기는 2012년에 30년 설계수명에 도달한다. 설계수명이후에 계속운전을 원하는 원전 사업자는 11개 인자를 포함하는 주기적안전성 평가 보고서를 제출해야 하며, 추가로 다음 사항에 대한 보고서를 제출해야 한다. (1) 경년열화 관리에 대한 범위 및 사전 검토 결과 (2) 경년열화 관리 프로그램 (3) 계속 운전기간을 포함하는 시간제한 경년열화 수명평가, (4) 운전경험 및 주요 연구결과의 반영. 이 연구에서는 상기 항목에 대한 54개의 규제지침이 개발되었다.