• 한국화재소방학회논문지
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• 제15권2호
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• pp.13-19
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• 2001

OECD는 규제개혁을 '보다 나은 규제'와 '규제폐지'로 구분하고, 환경 및 안전 분야의 규제는 지속되어야 한다고 강조한다. 반면에, 1990년대에 진행된 한국소방의 규제개혁은 일방적인 규제의 완화 또는 폐지라는 방향으로 진행되는 매우 기이한 현상을 나타내고 있다. 영국 소방예방법규의 경우, 1971년 화재예방법의 제정 이후 대형화재의 발생에 대응하여 지속적으로 예방법규를 강화해 오고 있으며, 1991년 건축령에서는 주택을 포함하여 모든 건물에 대하여 각층에 최소 한 개의 연기경보기를 설치할 것을 의무화하고 있다. 이 연구는 영국에서의 소방예방법규의 강화가 소방안전을 제고 시켰다는 것을 보여 주고 있는데, 이것은 한국소방에 시사하는 바가 크다. 한국소방의 경우 화제로부터 시민의 생명과 재산을 보호하기 위해서 '안전, 보건, 환경과 관련된 규제의 질을 개선한다'는 규제개혁위원회의 규제개혁 기본방향이 철저히 준수되어야 하며, 비록 규제개혁의 전체적 전략이 모든 규제를 감소시키는 것일지라도 안전에 관한 규정은 오히려 증가시킬 수 있다는 기본방향의 유연성이 확보되어야 한다.

• 한국화재소방학회논문지
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• 제16권4호
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• pp.1-6
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• 2002

정부는 행정규제를 폐지·개선하는데 중점을 두고 규제개혁을 추진해 왔다. 그러나 많은 규제개혁의 성과에도 불구하고 관료들의 행태 변화가 됫받침되지 못하거나, 이해관계집단의 반발로 인하여 규제개혁 체감도는 이에 미치지 못한다는 비판이 있었다. 이에 본 연구에서는 위험물 제조소 등의 현황을 파악하고, 소방행정의 규제순응에 대해서 피규제집단, 규제집단, 3차집단들을 대상으로 행정 규제를 제대로 인식하고 있는지, 또한 인정하고 준수하는지 등을 종합적·체계적으로 분석하여 소방규제의 제고방안을 제시하고자 하였다. 조사 결과, 전체적 순응도가 높게(전체 평균 80.0%) 평가되었다. 이는 현대사회의 급속한 산업경제의 발전과 의·식·주·에너지의 다양화를 뒷받침해온 위험물의 수요가 날로 증가하고 그에 따른 위험성의 증가에 따른 당연한 결과일 것이며, 피규제자, 규제자 그리고 일반국민들까지도 위험물 제조소등의 위험성을 인식하고 있음을 말해준다.

• Communications for Statistical Applications and Methods
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• 제25권4호
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• pp.341-354
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• 2018

The one of the principles described in ICH E9 is that only results obtained from pre-specified statistical methods in a protocol are regarded as confirmatory evidence. However, in multi-regional clinical trials, even when results obtained from pre-specified statistical methods in protocol are significant, it does not guarantee that the test treatment is approved by regional regulatory agencies. In other words, there is no so-called global approval, and each regional regulatory agency makes its own decision in the face of the same set of data from a multi-regional clinical trial. Under this situation, there are two natural methods a regional regulatory agency can use to estimate the treatment effect in a particular region. The first method is to use the overall treatment estimate, which is to extrapolate the overall result to the region of interest. The second method is to use regional treatment estimate. If the treatment effect is completely identical across all regions, it is obvious that the overall treatment estimator is more efficient than the regional treatment estimator. However, it is not possible to confirm statistically that the treatment effect is completely identical in all regions. Furthermore, some magnitude of regional differences within the range of clinical relevance may naturally exist for various reasons due to, for instance, intrinsic and extrinsic factors. Nevertheless, if the magnitude of regional differences is relatively small, a conventional method to estimate the treatment effect in the region of interest is to extrapolate the overall result to that region. The purpose of this paper is to investigate the effects produced by this type of extrapolation via estimations, followed by hypothesis testing of the treatment effect in the region of interest. This paper is written from the viewpoint of regional regulatory agencies.

• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.1029-1036
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• 2017

Objective: In the livestock industry, the regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth and meat production. We investigated the regulatory pathway of MyoD and its role in muscle differentiation in quail myoblast cells. Methods: The MyoD gene was mutated by the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology and single cell-derived MyoD mutant sublines were identified to investigate the global regulatory mechanism responsible for muscle differentiation. Results: The mutation efficiency was 73.3% in the mixed population, and from this population we were able to establish two QM7 MyoD knockout subline (MyoD KO QM7#4) through single cell pick-up and expansion. In the undifferentiated condition, paired box 7 expression in MyoD KO QM7#4 cells was not significantly different from regular QM7 (rQM7) cells. During differentiation, however, myotube formation was dramatically repressed in MyoD KO QM7#4 cells. Moreover, myogenic differentiation-specific transcripts and proteins were not expressed in MyoD KO QM7#4 cells even after an extended differentiation period. These results indicate that MyoD is critical for muscle differentiation. Furthermore, we analyzed the global regulatory interactions by RNA sequencing during muscle differentiation. Conclusion: With CRISPR/Cas9-mediated genomic editing, single cell-derived sublines with a specific knockout gene can be adapted to various aspects of basic research as well as in functional genomics studies.

• Safety and Health at Work
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• 제4권2호
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• pp.95-99
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• 2013

Background: Work-related stress is one of the major causes of occupational ill health. In line with the regulatory framework on occupational health and safety (OSH), adequate models for assessing and managing risk need to be identified so as to minimize the impact of this stress not only on workers' health, but also on productivity. Methods: After close analysis of the Italian and European reference regulatory framework and workrelated stress assessment and management models used in some European countries, we adopted the UK Health and Safety Executive's (HSE) Management Standards (MS) approach, adapting it to the Italian context in order to provide a suitable methodological proposal for Italy. Results: We have developed a work-related stress risk assessment strategy, meeting regulatory requirements, now available on a specific web platform that includes software, tutorials, and other tools to assist companies in their assessments. Conclusion: This methodological proposal is new on the Italian work-related stress risk assessment scene. Besides providing an evaluation approach using scientifically validated instruments, it ensures the active participation of occupational health professionals in each company. The assessment tools provided enable companies not only to comply with the law, but also to contribute to a database for monitoring and assessment and give access to a reserved area for data analysis and comparisons.

• 디지털융복합연구
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• 제14권3호
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• pp.143-150
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• 2016

본 연구는 조절초점의 조절효과를 중심으로 한 관광소셜커머스의 고객가치, 만족도, 지속적 이용의도 간의 관계를 규명한 후 고객가치와 만족도간 조절초점의 조절효과를 파악하였다. 실증분석결과로서 관광소셜커머스의 고객가치는 만족도에 부분적으로 긍정적 영향작용을 하는 것으로 나타났다. 그리고 만족도는 지속적 이용의도에 긍정적 영향을 미치는 것으로 나타났다. 조절초점의 조절효과 실증분석결과에 의거하면 고객가치와 만족도간의 조절초점의 조절효과는 부분적으로 긍정적 영향을 나타냈다. 이상과 같은 실증분석 결과에 의거하면 다음과 같은 시사점을 제시할 수 있겠다. 즉, 관광소셜커머스의 지속적 이용의도를 증진시키기 위해서는 관광객의 고객가치를 중시하고 또한 관광객의 향상초점을 촉진시켜야 할 것이다. 동시에 관광객의 예방초점에 대한 수준을 약화시킴으로써 관광소셜커머스의 지속적 이용의도를 도모하도록 하여야 할 것이다.

• 한국미생물·생명공학회지
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• 제38권4호
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• pp.475-480
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• 2010

Actinomycetes are Gram-positive soil bacteria and one of the most important industrial microorganisms due to superior biosynthetic capabilities of many valuable secondary metabolites as well as production of various valuable bioconversion enzymes. Among them are cytochrome P450 hydroxylase (CYP), which are hemoproteins encoded by a super family of genes, are universally distributed in most of the organisms from all biological kingdoms. Actinomycetes are a rich source of soluble CYP enzymes, which play critical roles in the bioactivation and detoxification of a wide variety of metabolite biosynthesis and xenobiotic transformation. Cyclosporin A (CyA), one of the most commonly-prescribed immunosuppressive drugs, was previously reported to be hydroxylated at the position of 4th N-methyl leucine by a rare actinomycetes called Sebekia benihana, leading to display different biological activity spectrum such as loss of immunosuppressive activities yet retaining hair growth-stimulating side effect. In order to improve this regio-selective CyA hydroxylation in S. benihana, previously-identified several secondary metabolite up-regulatory genes from Streptomyces coelicolor and S. avermitilis were heterologously overexpressed in S. benihana using an $ermE^*$ promoter-containing Streptomyces integrative expression vector. Among tested, SCO4967 encoding a conserved hypothetical protein significantly stimulated region-specific CyA hydroxylation in S. benihana, implying that some common regulatory systems functioning in both biosynthesis and bioconversion of secondary metabolite might be present in different actinomycetes species.

• Journal of Ginseng Research
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• 제12권2호
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• pp.135-144
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• 1988

갑상선호르몬 분비조절물질(TSH, DB cAMP, NaF, carbachol, isoproterenol, propranolol)과 인삼성분(total saponin, diol saponin, triol saponin)의 복합처리가 갑상선의 cAMP의 양에 미치는 영향을 알아보기 위하여, 흰쥐의 갑상선을 4일 또는 7일간 배양한 후 갑상선호르몬 분비조절물질과 인삼성분을 각각 복합처리, 또는 단독처리하여 cAMP의 양을 조사하였다. 인삼성분만을 처리한 경우, total saponin은 $10^{-5}$%(w/v), diol saponin과 triol saponin은 $10^{-4}$%(w/v)의 농도에서 각각 가장 높은 증가를 나타내었다. 갑상선호르몬 분비조절물질과 복합처리한 인삼성분의 농도는 위의 값으로 하였다. 복합처리한 경우, TSH에 대해서는 증가효과를 나타냈지만 그 양상은 작았다. Total saponin은 DBcAMP와 isoproterennol에 대해서는 증가효과를, carbachol과 propranolol에 대해서는 감소효과를 나타내었고, NaF에 대해서는 영향이 크지 않았다. Diol saponin과 triol saponin은 그 양은 다르지만 isoproterenol을 복합처리한 경우를 제외하고 diol saponin은 감소효과를, triol saponin은 증가효과를 보이는 상반작용을 나타내었다. 억제효과를 가지는 propranolol에 대해서도 diol saponin과 triol saponin은 상반되는 효과를 나타내었다. 인삼성분의 정상화작용은 NaF와 carbachol의 경우에도 두드러지게 나타났다. 이상의 결과들은 인삼성분이 갑상선호르몬의 생성과 분비에 관여하는 cAMP의 생성에 촉진 또는 억제를 가진다는 것을 나타내고 있다.

• IMMUNE NETWORK
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• 제11권1호
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• pp.11-41
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• 2011

The discovery of microRNA (miRNA) is one of the major scientific breakthroughs in recent years and has revolutionized current cell biology and medical science. miRNAs are small (19~25nt) noncoding RNA molecules that post-transcriptionally regulate gene expression by targeting the 3' untranslated region (3'UTR) of specific messenger RNAs (mRNAs) for degradation of translation repression. Genetic ablation of the miRNA machinery, as well as loss or degradation of certain individual miRNAs, severely compromises immune development and response, and can lead to immune disorders. Several sophisticated regulatory mechanisms are used to maintain immune homeostasis. Regulatory T (Treg) cells are essential for maintaining peripheral tolerance, preventing autoimmune diseases and limiting chronic inflammatory diseases. Recent publications have provided compelling evidence that miRNAs are highly expressed in Treg cells, that the expression of Foxp3 is controlled by miRNAs and that a range of miRNAs are involved in the regulation of immunity. A large number of studies have reported links between alterations of miRNA homeostasis and pathological conditions such as cancer, cardiovascular disease and diabetes, as well as psychiatric and neurological diseases. Although it is still unclear how miRNA controls Treg cell development and function, recent studies certainly indicate that this topic will be the subject of further research. The specific circulating miRNA species may also be useful for the diagnosis, classification, prognosis of diseases and prediction of the therapeutic response. An explosive literature has focussed on the role of miRNA. In this review, I briefly summarize the current studies about the role of miRNAs in Treg cells and in the regulation of the innate and adaptive immune response. I also review the explosive current studies about clinical application of miRNA.

• Bulletin of the Korean Chemical Society
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• 제31권6호
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• pp.1573-1576
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• 2010

Bacillus subtilis PerR is a metal-dependent peroxide-sensing transcription factor which uses metal-catalyzed histidine oxidation for peroxide-sensing. PerR contains two metal binding sites, one for structural $Zn^{2+}$ and the other for the regulatory/peroxide-sensing metal. Here we investigated the effect of mutations at both the structural and regulatory metal binding sites on the oxidation of either H37 or H91, two of the peroxide-sensing ligands. All four serine substitution mutants at the structural $Zn^{2+}$ site (C96S, C99S, C136S and C139S) exhibited no detectable oxidation at histidine residues. Two of the alanine substitution mutants at regulatory metal site (H37A and D85A) exhibited selective oxidation preferentially at the H91-containing tryptic peptide, whereas no oxidation was detected in the other mutants (H91A, H93A and D104A). Our results suggest that the cysteine residues coordinating structural $Zn^{2+}$ are essential for peroxide sensing by PerR, and that the C-terminal regulatory metal binding site composed of H91, H93 and D104 can bind $Fe^{2+}$, providing a possible explanation for the peroxide sensing mechanisms by PerR.