• Archives of Pharmacal Research
• /
• v.21 no.1
• /
• pp.35-40
• /
• 1998

Human cytochrome P450 4F2 shows high regioselectivity in hydroxylation of stearic acid and leukotriene $ B_4.$ As a first step of its regulation study, human cytochrome P450 4F2 genomic DNA was isolated from liver of a person who was administered clofibrate for 10 years. From Southern hybridization, restriction enzyme digestion and sequencing experiments, isolated genomic DNA fragment was found to contain around 32 Kb DNA and more than 20 Kb of $5^I$ end regulatory region. Sequences of the structural gene region revealed exon 1 and exon 2. Further regulation studies would elucidate the feedback mechanisms of the oxidative degradation of fatty acids, inflammatory response and the clearance of leukotriene B4 in the liver. Furthermore, regulation study of this gene could explain the species difference in responses to peroxisome proliferator and help in the safety evaluation of peroxisome proliferating chemicals to human being.

• Journal of Applied Biological Chemistry
• /
• v.42 no.2
• /
• pp.62-65
• /
• 1999

Iron regulatory proteins (IRPs) 1 and 2 bind with equally high affinity to specific RNA stem-loop sequences known as iron-responsive elements (IRE) which mediate the post-transcriptional regulation of many genes of iron metabolism. To study putative IRE-like sequences in RNA transcripts using the IRP-IRE interaction, Eight known genes from database were selected and the RNA binding activity of IRE-like sequences were compared to IRP-2. Among them, the IRE-like sequence in 3'-untranslational region (UTR) of divalent ration transporter-1 (DCT-1) shows a significant RNA binding affinity. This finding predicts that IRE consensus sequence present within 3'-UTR of DCT-1 might confer the regulation by IRP-2.

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.17 no.3
• /
• pp.61-69
• /
• 2009

This research examined the influence of status and regulatory focus on go-around decision making. The result showed that there was no difference between captains and first offers on the go-around decision making about various landing situations. But there was significant relationships between regulatory focus and go-around decision making. Prevention focus was positively related with uncertain situation, and runway situation. Promotion focus has positive relationship with deficiency situation but has negartive relationship with runway situation.

• Proceedings of the KSR Conference
• /
• 2009.05a
• /
• pp.1793-1798
• /
• 2009

Railway accidents have caused tremendous casualties and loss of property. In order to reduce the rate of accidents, it is important for the railway operators to elevate their recognition and give effective training as a safety education and training. Through the previous study, we developed regulatory guidance for developing safety education and training program. Then, three educational technology specialists verified it primarily. In this paper, we investigated present condition for running education and training system of domestic railway through result of In-depth interview. And we verified actual reflection through result of Checklist. Finally, improved regulatory guidance for developing safety education and training program was suggested in this study.

• Nuclear Engineering and Technology
• /
• v.37 no.3
• /
• pp.231-244
• /
• 2005

In recent decades, significant effort has led to risk-informed improvements to regulation. Performance-based approaches also promise significant gains in efficiency (level of safety versus effort). However, significant work remains to be done before performance-based approaches realize their full potential in regulation of nuclear power plants. This paper reviews key concepts related to performance-based regulation, discusses some applications of performance-based approaches, and identifies issues that still need to be addressed. Realistic, experience-based models of licensee performance are still lacking; this makes it difficult to assess the prospective effectiveness of any given regulatory approach, in light of the performance issues that it will actually face. Also, while 'compliance' is an intuitively straightforward concept to apply within a prescriptive implementation, its analog in a performance-based approach remains unclear. An overarching theme of the paper is that formal methods of decision analysis are very helpful in developing appropriate regulatory approaches, especially performance-based ones; this theme is illustrated at several points.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.11
• /
• pp.1755-1761
• /
• 2008

The present study revealed that Lrp, a leucine-responsive regulatory protein, is involved in the regulation of cadBA transcription through activation of $P_{cadBA}$. The influence of Lrp on $P_{cadBA}$ was mediated by CadC, and thereby, CadC was able to compensate for the lack of Lrp in the activation of $P_{cadBA}$. Western blot analyses and EMSA demonstrated that the cellular level of CadC was not significantly affected by Lrp, and that Lrp exerted its effect by directly binding to $P_{cadBA}$. These combined results suggested that CadC and Lrp function cooperatively to activate the $P_{cadBA}$ rather than sequentially in a regulatory cascade.

• BMB Reports
• /
• v.51 no.3
• /
• pp.106-118
• /
• 2018

How the organ size is adjusted to the proper size during development and how organs know that they reach the original size during regeneration remain long-standing questions. Based on studies using multiple model organisms and approaches for over 20 years, a consensus has been established that the Hippo pathway plays crucial roles in controlling organ size and maintaining tissue homeostasis. Given the significance of these processes, the dysregulation of the Hippo pathway has also implicated various diseases, such as tissue degeneration and cancer. By regulating the downstream transcriptional coactivators YAP and TAZ, the Hippo pathway coordinates cell proliferation and apoptosis in response to a variety of signals including cell contact inhibition, polarity, mechanical sensation and soluble factors. Since the core components and their functions of the Hippo pathway are evolutionarily conserved, this pathway serves as a global regulator of organ size control. Therefore, further investigation of the regulatory mechanisms will provide physiological insights to better understand tissue homeostasis. In this review, the historical developments and current understandings of the regulatory mechanism of Hippo signaling pathway are discussed.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 1999.04a
• /
• pp.17-24
• /
• 1999

The porvision against earthquakes and aseismic design of nuclear power plants (NPPs) in Korea are composed of four stages: site-selection, design, construction, and operation stages, Since regulatory criteria are strictly applied in each stage, the NPPs in Korea are believed to have a sufficient safety against maximum potential earthquakes. However, it has been recognized that those regulatory criteria borrowed from U.S. should be replace by Korea-specific ones by using earthquake data obtained from earthquake observation at and around NPP sites. Also, the government made a plan after the Yongwol and th Kyongju earthquakes that the regulatory body operates an independent earthquake network in order to reinforce the earthquake preparedness of NPPs. In compliance with the government's plan, this project is aiming at deployment of an earthquake motoring network composed of four seismic stations at NPP sites to record earthquake ground motions at NPP sites, to derive attenuation formulas of various ground motions and site-specific response spectra, and to develop structural intergrity assesment program.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2017.11a
• /
• pp.63-64
• /
• 2017

Protective coatings at nuclear power plants should be designed to withstand exposure to ambient conditions during normal operation or design-basis accidents. However, there was a change in the perception of the protective coating to the revision of the Regulatory Guidelines by the NRC in July 2000. In other words, maintenance guidelines have been strengthened in order to minimize the clogging of the cooling water system due to the substances in the containment building. Therefore, KHNP, the contractor and operator of the nuclear power plant, plans to develop the coating system for nuclear power plants in accordance with the regulation, and plans to develop its own coating expert.

• Nuclear Engineering and Technology
• /
• v.52 no.1
• /
• pp.69-77
• /
• 2020

After the Fukushima Daiichi accidents, public concerns on nuclear safety and the corresponding burden of nuclear power plant licensees are increasing. In order to secure public trust and enhance the rationality of current safety regulation, we develop a risk-informed decision making (RIDM) framework for the Korean regulatory body. By analyzing all the regulatory activities for nuclear power plants in Korea, eight action items are selected for RIDM implementation, with appropriate procedures developed for each. For two items in particular - the accident sequence precursor analysis (ASPA) and the significance determination process (SDP) - two customized risk evaluation software has been developed for field inspectors and probabilistic safety assessment experts, respectively. The effectiveness of the proposed RIDM framework is demonstrated by applying the ASPA procedure to 35 unplanned scrams and the SDP to 24 findings from periodic inspections.