• 대한물리의학회지
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• 제13권1호
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• pp.11-26
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• 2018

PURPOSE: The aim of this study was to review the effects of exercise intervention on blood glucose control in obese type 2 diabetic patients. METHODS: The PubMed and KERISS search engines were used and 61 papers that met the key questions were selected. RESULTS: Exercise is an effective intervention for the control of blood glucose in type 2 diabetic patients because it does not impair glucose transport in the skeletal muscle induced by muscle contractions. Insulin resistance, which is characteristic of type 2 diabetes, is caused by decreased insulin sensitivity or insulin responsiveness. Acute exercise improves the glucose metabolism by increasing the insulin-independent signaling pathways and insulin sensitivity in the skeletal muscle, and regular long-term exercise improves the skeletal muscle insulin responsiveness and systemic glucose metabolism by increasing the mitochondrial and GLUT4 protein expression in the skeletal muscle. CONCLUSION: The improvement of the glucose metabolism through exercise shows a dose-response pattern, and if exercise consumes the same number of calories, high intensity exercise will be more effective for the glucose metabolism. On the other hand, it is practically difficult for a patient with obese type 2 diabetes to control their blood glucose with high intensity or long-term exercise. Therefore, it will be necessary to study safe adjuvants (cinnamic acid, lithium) that can produce similar effects to high-intensity and high-volume exercises in low-intensity and low-volume exercises.

• Asian-Australasian Journal of Animal Sciences
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• 제30권5호
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• pp.743-748
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• 2017

Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs), the GFP-positive cells were sorted out by fluorescence-activated cell sorting procedure. Results: Through the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed. Conclusion: The knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.

• 한국의류산업학회지
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• 제15권2호
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• pp.173-181
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• 2013

This research analyzed the expression trend about the slit which composes the open space by the visual concept and then introduced the imbedded aesthetic value in it. Based on it, this work aims at showing the evidence on the slit as a utilizing tool for various design applications and providing the basic materials in order to develop the creative design production in the fashion area in the future. Specifically, for the theoretical background in this research, the concept of slit and its chronological changes were reviewed through literature. For the empirical analysis on the slit, a total of 226 designs with its application were analyzed from collection pieces in the four major collections including Paris, Milan, New York, and London from 2006S/S to 2012S/S. As a result of analysis, types of slit appeared as a slash, opening, slit, or a mix of them. These were applied to many different items of clothing, and among which, one-piece or dress was adopted most for application. In regard to space form, perpendicularity was used most frequently. But other various forms like cross and geometrical pattern were also used as well as curve, oblique line and horizontality. As to the arrangement of slit, single type was most frequently used. However, in addition to it, other types were also applied, producing both the functionality and the decorative detail such as bilateral symmetry, free irregularity, and a combination of regular and repeated layout which makes an effect of single pattern. Aesthetic values imbedded in slit were revealed as functionality, sensuality, spatiality, and decorativeness.

• 대한간호학회지
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• 제50권2호
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• pp.298-312
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• 2020

Purpose: This study had two objectives: 1) to develop a scale for the process of exercise engagement (SPEE) for prediabetic individuals (PDIs); 2) to validate a structural model for the process of exercise engagement for PDIs. Methods: A cross-sectional survey with simple random sampling was conducted from September 2013 to December 2015 (in Taiwan). A total of 310 PDIs were enrolled for scale development and model validation via item analysis, factor analyses, and structural equation modeling. The Kuo model was used as the basis for developing the Chinese version of the SPEE for PDIs. Results: The SPEE contains five subscales with a total of twenty-one items that account for 54.9% to 65.9% of the total variance explained for assessing participants' process of engagement during exercise. For Kuo model validation, the model measures indicated goodness of fit between the Kuo model and sample data. Analysis further revealed a direct effect between the creating health blueprints (CHB) stage and the spontaneous regular exercise (SRE) stage (β=.60). Conclusion: The SPEE includes five subscales for assessing the psychological transition and behavioral expression at each stage of the process of exercise engagement for PDIs. The SPEE for people with prediabetes provides deeper insights into the factors of behavioral change stages that are required to initiate long-term health care outcomes and avoid developing diabetes. These insights are significant as they allow for patient-specific mapping and behavior modification to effect exercise.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 추계종합학술대회
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• pp.504-507
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• 2003

오늘날 XML이 전자 상거래 시장에 널리 수용하여 사용되고 있다. 하지만 XML 문서는 논리적으로 동일한 의미를 물리적으로 여러 다른 형태로 나타낼 수 있는 표현의 자율성이 존재하기 때문에 XML 전자서명과 같은 물리적 형태로써 유효성을 판단하는 응용프로그램에서는 문제가 발생할 수 있다. 따라서 이런 문제점을 해결하기 위하여 W3C에서는 논리적으로 동일한 의미의 XML 문서를 물리적으로 동일하게 변환시키도록 XML 정규화 알고리즘을 제안하여 사용하도록 권고하고 있다. 이에 본 논문에서는 W3C에서 권고한 XML 정규화 알고리즘을 수행하는 시스템을 설계 및 구현함으로써, 좀 더 정교하고 정규화된 문서로 변형하여 W3C 표준을 따르는 다른 응용 시스템과의 상호운용이 가능하다. 또한 웹 서비스를 위한 전자서명 시스템에서의 사용이 용이하며 웹 서비스 상호 운용성을 위한 XML 문서 교환 시 물리적 동일성이 요구되는 여러 시스템에서 다양한 방면으로 사용될 것으로 사료된다. 뿐만 아니라 국제적 인코딩 스킴과 국내 인코딩 스킴인 EUC-KR과의 변환기능을 추가함으로써 국내 실정에 맞는 XML 정규화 알고리즘이 될 것이며, 이는 국제적 상호 운용성 확보의 기반 기술이 될 것이다.

• 정보과학회 논문지
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• 제44권11호
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• pp.1178-1193
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• 2017

지식 도메인에 대한 집단적인 패턴에서 시스템의 행위를 이해하는 것은 중요하다. 그러나 시스템의 크기, 상호작용의 복잡성으로 집단 행위를 표현하는데 구조적인 어려움이 있으며, 상태 폭발 문제가 발생한다. 또한, 다른 시스템과의 합성은 시스템의 크기와 복잡성이 기하급수적으로 증가하기 때문에 현실적으로 어렵다. 본 논문은 도메인 공학의 새로운 개념인 행위 온톨로지를 이용하여, 집단 행위를 모델링하는 실용적인 방법을 제시한다. 첫째, 액티브 온톨로지를 통해 시스템의 행위를 정의한다. 둘째, 행위는 일반 정규 표현식이라고 불리는 정량화된 추상 격자 구조로 표현된다. 셋째, 격자는 공통된 요소를 기반으로 다른 격자와 합성할 수 있다. 이 방법은 시스템 복잡성을 줄이기 위해, 시스템 상태를 최소화하는 것뿐만 아니라 집단 패턴에서 시스템 동작을 표현하는 혁신적인 방법 중 하나일 수 있다. 이 방법을 구현하기 위해, PRISM 프로토타입 도구를 ADOxx 메타 모델링 플랫폼으로 개발하였다.

• Nutrition Research and Practice
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• 제10권6호
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• pp.623-628
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• 2016

BACKGROUND/OBJECTIVES: Obesity-induced steatohepatitis accompanied by activated hepatic macrophages/Kupffer cells facilitates the progression of hepatic fibrinogenesis and exacerbates metabolic derangements such as insulin resistance. Heme oxyganase-1 (HO-1) modulates tissue macrophage phenotypes and thus is implicated in protection against inflammatory diseases. Here, we show that the flavonoid quercetin reduces obesity-induced hepatic inflammation by inducing HO-1, which promotes hepatic macrophage polarization in favor of the M2 phenotype. MATERIALS/METHODS: Male C57BL/6 mice were fed a regular diet (RD), high-fat diet (HFD), or HFD supplemented with quercetin (HF+Que, 0.5g/kg diet) for nine weeks. Inflammatory cytokines and macrophage markers were measured by ELISA and RT-PCR, respectively. HO-1 protein was measured by Western blotting. RESULTS: Quercetin supplementation decreased levels of inflammatory cytokines ($TNF{\alpha}$, IL-6) and increased that of the anti-inflammatory cytokine (IL-10) in the livers of HFD-fed mice. This was accompanied by upregulation of M2 macrophage marker genes (Arg-1, Mrc1) and downregulation of M1 macrophage marker genes ($TNF{\alpha}$, NOS2). In co-cultures of lipid-laden hepatocytes and macrophages, treatment with quercetin induced HO-1 in the macrophages, markedly suppressed expression of M1 macrophage marker genes, and reduced release of MCP-1. Moreover, these effects of quercetin were blunted by an HO-1 inhibitor and deficiency of nuclear factor E2-related factor 2 (Nrf2) in macrophages. CONCLUSIONS: Quercetin reduces obesity-induced hepatic inflammation by promoting macrophage phenotype switching. The beneficial effect of quercetin is associated with Nrf2-mediated HO-1 induction. Quercetin may be a useful dietary factor for protecting against obesity-induced steatohepatitis.

• The Plant Pathology Journal
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• 제29권2호
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• pp.209-220
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• 2013

Root colonization by Pseudomonas chlororaphis O6 induces systemic drought tolerance in Arabidopsis thaliana. Microarray analysis was performed using the 22,800-gene Affymetrix GeneChips to identify differentially-expressed genes from plants colonized with or without P. chlororaphis O6 under drought stressed conditions or normal growth conditions. Root colonization in plants grown under regular irrigation condition increased transcript accumulation from genes associated with defense, response to reactive oxygen species, and auxin- and jasmonic acid-responsive genes, but decreased transcription factors associated with ethylene and abscisic acid signaling. The cluster of genes involved in plant disease resistance were up-regulated, but the set of drought signaling response genes were down-regulated in the P. chlororaphis O6-colonized under drought stress plants compared to those of the drought stressed plants without bacterial treatment. Transcripts of the jasmonic acid-marker genes, VSP1 and pdf-1.2, the salicylic acid regulated gene, PR-1, and the ethylene-response gene, HEL, also were up-regulated in plants colonized by P. chlororaphis O6, but differed in their responsiveness to drought stress. These data show how gene expression in plants lacking adequate water can be remarkably influenced by microbial colonization leading to plant protection, and the activation of the plant defense signal pathway induced by root colonization of P. chlororaphis O6 might be a key element for induced systemic tolerance by microbes.

• Food Science and Biotechnology
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• 제16권6호
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• pp.910-917
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• 2007

The aim of this study is to determine the anti-obesity effects of genistein and exercise, separately and in combination, in mice. Fifty male C57BL/6J mice were divided into 5 treatment groups: normal diet (ND), high fat diet (HD), high fat diet with exercise (HD+Ex), high fat diet with 0.2% genistein (HD+G), high fat diet with 0.2% genistein, and exercise (HD+G+Ex). They were allowed free access to feed and water, and exercised mice engaged in swimming on a regular basis for 12 weeks. Genistein supplemented mice gained less weight, had lower energy intake, better lipid profiles, and lower leptin than the HD mice. Furthermore, when genistein was combined with exercise (HD+G+Ex) the effects were even greater. HD, HD+Ex, and HD+G mice exhibited increased hepatic CPT-1 mRNA expression. Therefore, genistein and exercise has anti-obesity effects, as shown by changes in body weight, fat accumulation, energy intake, and leptin levels.

• 한국콘텐츠학회논문지
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• 제15권11호
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• pp.1-9
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• 2015

감성인식을 규칙적으로 제어하고 변화하려는 감성컴퓨터 연구에 관심이 집중되고 있다. 따라서 색채 감성컴퓨팅의 정량화한 객관적인 평가 방식 적용이 필수적인 상황이다. 이에 본 논문은 이미지 감성인식을 숫자화한 표현방식으로 색채 디지털 감성컴퓨팅 계산을 적용한다. 감성컴퓨팅 연구방식은 이미지에 집중된 감성인식인 색채 속성으로 구성하고, 색채 감성속성은 색상, 명도, 채도로 구분한다. 감성속성의 비중톤 증감에 따른 감성점수와 가중치를 감성식에 적용하여 계산한다. 감성계산식은 쾌정도(X축), 긴장도(Y축)를 계산한다. 그리고 감성좌표에 쾌정도(X축), 긴장도(Y축)의 교차점을 감성점으로 위치한다. 감성좌표는 러셀의 핵심 효과(Core Affect)를 활용한다. 감성점 위치에서 대표감성크기와 감성상관관계를 숫자화하고, 색채 감성컴퓨팅을 통해 정량화한다.