• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.317-322
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• 1997

In order to investigate vigor of rice seed pregerminated, some organic and inorganic component content, response of ecotypes, and effect of gibberellin treatment, under different levels of hastening of germination, were examined. Protein content was high in dried seed, the larger size in length of pregerminated seed, the less protein content, but lipid content was a reverse tendency to protein one. Potassium content was low in dried seed, the larger size in length of pregerminated seed, the more potassium content, but increase dramatically at above 10 mm in length of sprout. Magnesium content showed a same tendency to potassium one. The ability of regermination was high in early maturing cultivar, but almost lost in the pregerminated seeds with above 5 mm in length of sprout. The regermination percentage of dried seed was lower than that of pregerminated seed with 0.1∼0.5 mm in length, but was similar to that of pregerminated one with 1∼3 mm in length of sprout. The effect of gibberellin on regermination percentage was highest at 10 ppm and showed high with small sized one of pregerminated seed. The effect of gibberellin on plant height, fresh weight, and dry weight showed a same tendency to regermination percentage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.115-120
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• 1994

To find out the effects of preharvest sprouted degrees of barley on yield, grain quality and germination rate, various sprouted grains were planted. The grains sprouted upto 2 mm and 4mm of root lengths showed regerminating rates of 68% and 49% respectively, and those above 4mm of root length decreased seriously in regerminating rate. First of all, the PI (promptness index) of sprouted barley compared with that of intact barley decreased conspicuously even in 2mm of root length. Grain yield decreased inversely with the growth of root lengths of sprouted grains. The times required to polish the naked barley up to polishing yield of 72% and husked one upto that of 64% were shortened inversely with the root lengths of sprouted grains. The rate of split kernel against sound one after polishing increased greatly by 17.6% to 36% in sprouted barley compared to 2% to 3.5% of the control which presoaked in water for 2 hours. Whiteness indexes of polished kernels of the sprouted barley and the control were higher than that of intact one. The index, however, was lowered inversely with root lengths in sprouted barley.

• KSBB Journal
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• v.9 no.2
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• pp.200-210
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• 1994

In batch bioreactor fermentations for cyclosporin A (CyA) production, good potential for bioprocess improvement was demonstrated in the immobilized cell system, providing appreciably better utilization of the catalytic activity of the biomass than the freely suspended cells, especially during the exponential phase. When concentrated nutrient medium was added pulsely during the exponential phase of cell growth(at hour 139 of fermentation), reactivation and regermination in both immobilized and suspended cell cultures were observed to contribute to the longevity of CyA production, maintaining maximum CyA titre until 250 hours of fermentation. Contrarily, simple batch fermentations without any supplement of medium in both systems showed repid decrease in CyA concentrations during the late stationary phase. Notably, the CyA yield coefficient $(Y_p/x)$ for the immobilized cell system was maintained quite high even after the pulse addition of the concentrated full medium, reaching almost 80% of the level attained during the exponential phase. This is in sharp contrast when compared with the corresponding value of 58% in the case of parallel-suspended cells. This pattern of CyA production resulted in considerably enhanced CyA production in the immobilized cell system, reaching almost 2 time higher maximum CyA production in comparison with the free cell system.