• Title/Summary/Keyword: Regeneration

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A Study of Hox Gene Expression Profile During Murine Liver Regeneration

  • Boyeon-Youn;Kim, Byung-Gyu;Kim, Myoung-Hee
    • Biomedical Science Letters
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    • v.9 no.1
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    • pp.1-8
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    • 2003
  • Liver is an organ having an ability to regenerate by itself when it is damaged or removed. Since the research on the liver regeneration so far was regarding on the cellular multiplications not the formation of the shape, we intended to analyze the expression pattern of Hox genes during liver regeneration. RNA samples isolated from liver at the time of partial hepatectomy, 4 hours as well as 3 days later following regeneration were used to perform RT-PCR with Hox-specific degenerate primers. The PCR products were cloned, sequenced and analyzed through BLAST program. Genes belonging to the AbdB type Hox genes (paralogous groups IX-XIII) expressed predominantly during regeneration, while the other group (I-VII), especially Hoxal and bl seemed to be expressed continuously before and after regeneration. These data altogether imply that paralogous group IX and X genes including Hoxa10 and d10 seemed to be regeneration specific genes of liver.

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A Study on the Diagnosis Indicators and checklist for Urban Regeneration Projects by LH (LH형 도시재생사업 진단 지표 및 체크리스트 개발)

  • Park, Dong Sun;Lee, Young Eun;Kim, Ho Chang
    • Land and Housing Review
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    • v.9 no.2
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    • pp.1-7
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    • 2018
  • The purpose of this study is to suggest diagnosis indicators and checklist for urban regeneration projects by Korea Land & Housing Corporation(LH). There are already deprivation indices in the Urban Regeneration Act but not any additional guidelines in the practical aspect. In order to use the diagnosis indicators, the central government should supply more specific checklist to the actors in the regeneration field. The key actor of many stakeholders is LH as an operator and implementer in the regeneration projects. So far, LH has developed housing and cities and there haven't been any obvious changes to realize public benefit in the deteriorated area. From now on, It has to plan, implement, and manage a lot of regeneration projects entirely. Therefore, It is necessary to develope and apply the diagnosis indicators and checklist based on projects. This paper came up with the 6 factors related with LH business field : housing, urban infrastructure, public service, private service, environment, and smart city. For these, 32 diagnosis indicators and 72 checklists were selected that can include both physical and qualitative indicators. These can be used not only for the selection of regeneration projects but also for the process monitoring such as planning and implementation.

A Study on the Berlin Waterfront Development for Urban Regeneration -Focused on the case of Wasserstadt Berlin-Oberhavel, Berlin, Germany-

  • Park, Jong-Ki
    • KIEAE Journal
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    • v.17 no.3
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    • pp.23-34
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    • 2017
  • Purpose: The purpose of this study is to explore the relationship between suburban waterfront und urban regeneration strategies. Specifically, This paper investigates how urban design strategies for suburban waterfront has been conceptualized and realized in a case-based research study of the regeneration of suburban waterfront in Berlin, Germany. Since Germany's reunification, Berlin was faced a wide variety of challenges and has been transformed into a new capital city. First of all, Wasserstadt Berlin-Oberhavel was a key development task for the urban regeneration in Berlin. The Wasserstadt Berlin-Oberhavel project with over 207 hectares will provide new residential urban areas, office spaces, educational facilities and cultural facilities. In details, 3,640 apartments with 12,700 units and around 4,800 jobs will be created. Method: The study consists of five sections. The first and second section are divided into two stages:"waterfront definition in urban design" and "urban design strategy in waterfront". The third section explores the waterfront development process of Wasserstadt Berlin-Oberhavel. The fourth section aims to investigate the strategies for urban regeneration as well as waterfront development. Finally, the fifth provides some of the key policy challenges for Urban Regeneration in suburban waterfront area. Result: The results showed that urban regeneration strategies in suburban waterfront have a substantial impact on all two dimensions: relationship between waterfront and urban context, relationship between waterfront and natural environment.

Quantitative trait loci (QTLs) detection for plant regeneration ability from seed culture in rice (Oryza sativa L.)

  • Liu, Meihan;Sohn, Jae-Keun
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.169-174
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    • 2012
  • Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

The Natural Regeneration and Stand Characteristic of the Korean Fir Stand in Nae Sorak - A Study about the Concept of the Natural Regeneration in a Natural Fir Forest - (내설악 전나무림 천연갱신과 임분특성 - 전나무 자연림에서 천연갱신에 대한 개념적 고찰을 중심으로 -)

  • Youn, Young-Il
    • Korean Journal of Environmental Biology
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    • v.27 no.2
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    • pp.176-182
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    • 2009
  • We surveyed the total 30 sites in Gilgol, Nae Sorak for the patterns of the natural regeneration of the korean fir in connection with the characteristic of the stand. The natural regeneration in a forestrical meaning is not found in natural forest but mainly in the anthropogenically already disturbed area. It may be inferred from this fact that the anthropogenic disturbance can give more better condition for a natural regeneration. The regeneration phase in the natural forest is not so distinctive as the artificial forest, and shows itself transiently. And it may be also said that a shift in generation is only a part of the change of the stand phase. The concept of the natural regeneration will lose its meaning, in so far as the patch dynamic determines the phase of the stand, and the main tree species are shade-tolerant abies.

Optimization of Regeneration Condition Under Agrobacterium-mediated Transformation in in vitro Cultured Korean Soybean

  • Kantayos, Vipada;Bae, Chang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.36-36
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    • 2018
  • Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

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A Study on Environment of Daldongnae and Regeneration in Jeonju - Focused on the Jaman-maeul area in Jeonju City - (전북 전주시 달동네 환경 분석과 마을 가꾸기 방안에 관한 연구 - 전북 전주시 교동 자만마을을 중심으로 -)

  • Lee, Sung-Kyun
    • Journal of the Korean Institute of Rural Architecture
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    • v.15 no.3
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    • pp.41-48
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    • 2013
  • The study aims to figure out the problems of Daldongnae (hillside shantytown) in Jaman-dong, Jeonju, and to propose the countermeasures for village regeneration. Based on the survey of current conditions in the area and the case study of successful regeneration projects, this study suggests regenerating concepts for the community. The study on the current state analyzes that Jaman-dong is faced with the population decline, especially among the young, the lack of community facilities and infrastructure, and the unfavorable situation in the residential environment. The case study involves three successful Daldongnae regeneration projects: the culture-based regeneration of Cultural Village (in Gamcheon-dong, Busan), the regeneration focused on healthcare and education of Ibagugil (in Choryang-dong, Busan), and the community-led regeneration of Rainbow Village (in Dae-dong, Daejeon). The regenerating concepts for the community are as follows: utilizing the existing local community center as a gathering place for the residents, furnishing a cultural space for a growing number of visitors, adding new parking space, installing convenient facilities in order to provide better pedestrian environment for the disabled and elderly, improving street landscapes, and establishing citizen participation programs.

MET1-Dependent DNA Methylation Represses Light Signaling and Influences Plant Regeneration in Arabidopsis

  • Shim, Sangrea;Lee, Hong Gil;Seo, Pil Joon
    • Molecules and Cells
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    • v.44 no.10
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    • pp.746-757
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    • 2021
  • Plant somatic cells can be reprogrammed into a pluripotent cell mass, called callus, which can be subsequently used for de novo shoot regeneration through a two-step in vitro tissue culture method. MET1-dependent CG methylation has been implicated in plant regeneration in Arabidopsis, because the met1-3 mutant exhibits increased shoot regeneration compared with the wild-type. To understand the role of MET1 in de novo shoot regeneration, we compared the genome-wide DNA methylomes and transcriptomes of wildtype and met1-3 callus and leaf. The CG methylation patterns were largely unchanged during leaf-to-callus transition, suggesting that the altered regeneration phenotype of met1-3 was caused by the constitutively hypomethylated genes, independent of the tissue type. In particular, MET1-dependent CG methylation was observed at the blue light receptor genes, CRYPTOCHROME 1 (CRY1) and CRY2, which reduced their expression. Coexpression network analysis revealed that the CRY1 gene was closely linked to cytokinin signaling genes. Consistently, functional enrichment analysis of differentially expressed genes in met1-3 showed that gene ontology terms related to light and hormone signaling were overrepresented. Overall, our findings indicate that MET1-dependent repression of light and cytokinin signaling influences plant regeneration capacity and shoot identity establishment.

A Study on the Regeneration of Low-rise Residential Village through Future Heritage in Jeonju - Focusing on the Case of Village Regeneration through the Future Heritage of Seohak-dong Artist Village - (전주시 미래유산을 통한 저층 주거지 마을 재생 연구 - 서학동 예술인 마을 미래유산을 통한 마을 재생사례를 중심으로 -)

  • Kim, Jun-Young
    • Journal of the Korean Institute of Rural Architecture
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    • v.25 no.2
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    • pp.9-16
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    • 2023
  • This study attempted to analyze the aspect of seeking a characteristic regeneration of low-rise residential areas through the Urban Regeneration New Deal Project in Seohak-dong Art Village in Jeonju, while the urban regeneration new deal projects are being carried out in various regions, nationwide. Time-series changes in aerial photographs in the study area, age, number of floors, and uses of buildings were examined. The main street structure, lots, and changes in buildings were reviewed, and through this, spatial zones that could extract the unique characteristics of the target area were classified. The characteristics of each area was linked to the proposal of an appropriate regeneration plan through the analysis. This study is meaningful in showing a case of characteristics of low-rise residential areas regeneration plan suitable for the specific region while the number of old low-rise residential areas is increasing.