• YAKHAK HOEJI
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• v.45 no.2
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• pp.227-236
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• 2001

The complexity and variability of both the biologicals and the bioassays used to test them led to the use of the reference standard- a sample of the product of defined purity and potency, against which all preparations of that product must be calibrated. In order to prepare and establish KFDA reference standard for recombinant human growth hormone (somatropin), somatropin substance was filled in ampoules in National Institute for Biological Standards and Control (NIBSC). The candidate KFDA reference standard for somatropin (designated as 98/674) was evaluated to determine the suitability of serving as a KFDA reference standard for somatropin by the collaborative study, in which 10 laboratories participated. Physicochemical analysis and in vivo bioassay were performed by direct comparison with the international somatropin standard 88/624. 98/674 was identified as somatropin by SDS-PAGE, IEF, peptide mapping, and HPLC. Determination of somatropin content by SE-HPLC yielded a mean estimate of 2.01 mg somatropin per ampoule. Data from the study also yielded mean values of 0.39 $\pm$ 0.26% for high molecular weight impurities by SE-HPLC and mean values of 2.13 $\pm$ 1.29% for somatropin related proteins by RP-HPLC. Estimates of relative potency by weight gain bioassay in the hypophysectomised rats showed that relative potency of KS 98/674 was 1.07 aganist IS 88/624. Based on the results of the collaborative study, the candidate reference standard for somatropin is suitable to serve as a KFDA reference standard for somatropin.

• The Korean Journal of Nuclear Medicine Technology
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• v.27 no.2
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• pp.133-136
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• 2023

Purpose: Prolactin in the blood is separated into three types, and over 90% of prolactin presents as a double monomer (23 KDa). Rarely, it can exist in the size of big prolactin (150 KDa), which is called macroprolactin and is known as an autoantibody complex. When macroprolactin accounts for more than 60% of prolactin in the blood, it is called macroprolactinemia. The presence of such macroprolactin was first reported in a patient with hyperprolactinemia but without typical symptoms. Macroprolactinemia is emerging as an important cause of idiopathic hyperprolactinemia. The polyethylene glycol (PEG) precipitation method using the property of precipitating large-molecular-weight proteins is simple and recently has been widely used as a screening test. The results are in good agreement with the results of gel chromatography. The purpose of this study was to confirm the measurement method and reference value verification of monomeric prolactin in blood prolactin using the PEG precipitation method. Materials and Methods: For 40 examinees who visited the Gangnam Center of Seoul National University Hospital in 2021, the prolactin level was verified using radioimmunoassay (RIA). For macroprolactinemia PEG precipitation method, 25% PEG (molecular weight 6000kDa) solution and serum were mixed in equal amounts in a test tube, then left at room temperature for 20 minutes and centrifuged at 4℃ for 30 minutes (1500g). The prolactin level was measured in the supernatant. Results : After confirming that more than 90% of the 40 tested samples within the reference range <25 ng/mL, the same value as the reference value for prolactin was applied. Since the concentration of monomeric prolactin in serum from which macroprolactin has been removed from blood is diluted 1:1 with PEG, our laboratory is currently reporting the result by multiplying the result by a dilution factor of 2. Conclusion: Radioimmunoassay using PEG precipitation method using the property of precipitating large molecular weight proteins is simple and effective for quantitative measurement of monomeric prolactin in blood prolactin.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.30B no.10
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• pp.95-104
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• 1993

IPA(interpattern association) model is a method of feature extraction using a neural network. Even in the case that the reference patterns are simuklar to one another, this model can recover the reference patterns effectively. However, when the pattern whose feature pixels are lost is used as input, this model can not guarantee perfect recovery of the reference pattern. It is proposed a improved interpattern association(IPA) model for the feature extraction using neural network. The improved IPA model that combines the first interconnection weight matrix of the IPA model with the second additional weight matrix is proposed here to overcome the recovery problem of the original IPA model. The results of computer simulation and optical experiment are advanced.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.6
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• pp.111-118
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• 2000

The design of reference trajectory with respect to drag acceleration is necessary to decelerate from hypersonic speed safely after atmosphere re-entry of space vehicle. The re-entry guidance design involves trajectory optimization, generation of a reference drag acceleration profile with the satisfaction of 6 trajectory constraints during the re-entry flight. This reference drag acceleration profile can be considered as the reference trajectory. The cost function is composed of the accumulated total heating on vehicle due to the reduction of weight. And a regularization is needed to prevent optimal drag profile from varying too fast and achieve realized trajectory. This paper shows the relations between velocity, drag acceleration and altitude in drag acceleration profile, and how to determine the reference trajectory.

• Nuclear Engineering and Technology
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• v.31 no.6
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• pp.23-38
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• 1999

This study addresses the reference spent fuel and its characteristics for developing a geological repository concept. As a disposal capacity of the reference repository system to be developed, spent fuel inventories were projected based on the basis of the Nuclear Energy Plan of the Long-term National Power Program. The reference spent fuel encompassing a variability in characteristics of all existing and future spent fuels of interest was defined. Key parameters in the reference fuel screening processes were the nuclear and mechanical design parameters and the burnup histories for existing spent fuels as of 1996 and for future spent fuels with the more extended burnup the initial enrichment and its expected turnup. The selected reference fuel was characterized in terms of initial enrichment, bumup, dimension, gross weight and age. Also the isotopic composition and the radiological properties are quantitatively identified. This information provided in this study could be used as input for repository system development and performance assessment and applied in fuel material balance evaluation for the various types of back-end fuel cycle studies.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.31A no.7
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• pp.55-62
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• 1994

Interpattern association (IPA) model which the interconnection weight matrix(IWM) is constructed by the association between patterns is effective in similar pattern recognitions. But, if the number of reference patterns is increased, the ability of recognition is decreased. Using a hierarchical recognition algorithm which adopts the tree search strategy, we classified reference patterns into sub-groups by similarity. In IPA model, if input includes random noise we make it converge to reference pattern by means of input includes random noise we make it converge to reference pattern by means of increasing the number of pixels of prohibited state in IWM. In relation to reference patterns the pixel of prohibited state made partially prohibited state of no connected state using which is not included common and feature regions by each reference patterns.

• Proceedings of the Acoustical Society of Korea Conference
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• 1994.06c
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• pp.133-137
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• 1994

In this paper, a method for designing an optimal weight function for the weighted cepstral distance measure is proposed. A conventional weight function or cepstral lifter is obtained eperimentally depending on the spectral components to be emphasized. The proposed method minimizes the error between word reference patterns and the traning data. To compare the proposed optimal weight function with conventional function, speech recognition systems based on Dpynamic Time Warping and Hidden Markov Models were constructed to conduct speaker independent isolated word necogination eperiment. Results show that the proposed method gives better performance than conventional weight functions.

• Journal of the Korean Vacuum Society
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• v.22 no.5
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• pp.231-237
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• 2013

New method of volume measurement for reference weights of a pressure balance using a gas pycnometer is proposed. The result of volume measurement of proposed method shows the uncertainties of approximately 0.2% at the level of confidence of 95% for reference weights in the ranges of 1 kg, 2 kg, and 5 kg. This measuring system consists of a sample chamber, an expansion chamber, a precision pressure gage, a precison thermometer, a vacuum pump, and helium as a medium gas. The measurement principle of this proposed method is based on Boyle's law. This method will contribute a reliability of the volume measurements of reference weights for a pressure balance to the national measurement standard.

• Journal of Positioning, Navigation, and Timing
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• v.11 no.4
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• pp.369-379
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• 2022

In weighted k-nearest neighbor (WkNN)-based Fingerprinting positioning step, a process of comparing the requested positioning signal with signal information for each reference point stored in the fingerprint DB is performed. At this time, the higher the number of matched base station identifiers, the higher the possibility that the terminal exists in the corresponding location, and in fact, an additional weight is added to the location in proportion to the number of matching base stations. On the other hand, if the matching number of base stations is small, the selected candidate reference point has high dependence on the similarity value of the signal. But one problem arises here. The positioning signal can be compared with the repeater signal in the signal information stored on the DB, and the corresponding reference point can be selected as a candidate location. The selected reference point is likely to be an outlier, and if a certain weight is applied to the corresponding location, the error of the estimated location information increases. In order to solve this problem, this paper proposes a WkNN technique including an outlier removal function. To this end, it is first determined whether the repeater signal is included in the DB information of the matched base station. If the reference point for the repeater signal is selected as the candidate position, the reference position corresponding to the outlier is removed based on the clustering technique. The performance of the proposed technique is verified through data acquired in Seocho 1 and 2 dongs in Seoul.

• Journal of Radiation Protection and Research
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• v.8 no.1
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• pp.1-14
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• 1983

For the purpose of prediction and protection of radiation effects on the Korean people, we have collected autoptic data from the National Institute of Scientific Investigation. The mass of organs and the size of brains measured is analysed for 1,921 Korean people. The results obtained are as follows; 1. The weight of the kidney in the Reference Korean is 251.6g in male and 227.7g in female. 2. The weight of the lung in the Reference Korean is 1,204.4g in male and 957.4g in female. 3. The weight of the heart in the Reference Korean is 348.8g in male and 301.6g in female. 4. The weight of the liver in the Reference Korean is 1,863.9g in male and 1,610.9g in female. 5. The weight of the pancreas in the Reference Korean is 56.4g in male and 54.0g in female. ,6. The weight of the spleen in the Reference Korean is 67.3g in male and 58.2g in female. 7. The anteroposterior diameter, transverse diameter and vertical diameter of the brain in the Reference Korean are 17.6cm, 15.5cm and 8.6cm in male, respectively, and 17.7cm, 15.4cm and 8.2cm in female, respectively.