• 한국지반공학회논문집
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• 제20권5호
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• pp.27-35
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• 2004

본 연구의 목적은 불포화토의 거동을 대변하는 흙-수분 특성곡선을 결정하는 데 있어서 액성한계상태를 이용한 비교상태접근법(reference state approach)의 적용 가능성을 확인하고 이를 바탕으로 하는 예측방법을 제안하는 데 있다. 세립잔적토를 사용하여 각기 다른 간극비로 다짐된 여러 개의 시료로부터 흙-수분 특성곡선을 구하였다. 실험결과를 바탕으로, 흙-수분 특성곡선을 예측하는 데 있어서 액성한계상태를 비교상태로 사용할 수 있음을 증명하였다. 마지막으로, Fredlund와 Xing(1994)이 제안한 흙-수분 특성곡선식을 사용하여 실험결과를 회귀분석 함으로서 액성한계를 비교상태로 하여 세립잔적토의 흙-수분 특성곡선을 예측할 수 있는 방법을 제안하였다.

• Journal of Veterinary Science
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• 제23권5호
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• pp.32.1-32.11
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• 2022

Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

• 전자공학회논문지A
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• 제31A권7호
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• pp.55-62
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• 1994

Interpattern association (IPA) model which the interconnection weight matrix(IWM) is constructed by the association between patterns is effective in similar pattern recognitions. But, if the number of reference patterns is increased, the ability of recognition is decreased. Using a hierarchical recognition algorithm which adopts the tree search strategy, we classified reference patterns into sub-groups by similarity. In IPA model, if input includes random noise we make it converge to reference pattern by means of input includes random noise we make it converge to reference pattern by means of increasing the number of pixels of prohibited state in IWM. In relation to reference patterns the pixel of prohibited state made partially prohibited state of no connected state using which is not included common and feature regions by each reference patterns.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2004년도 춘계학술발표회
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• pp.146-153
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• 2004

The goals of this study are to investigate the feasibility of the reference state approach in determining the generalized soil-water characteristic curve that is essential for characterization of unsaturated soil behavior. The soil-water characteristic curves are obtained from a number of specimens of fine-grained residual soils compacted with different void ratios. Based on the experimental test results, the feasibility of using the liquid limit state as the reference state for predicting the soil-water characteristic curve are verified. Finally, through the regression analysis of experimental data using the equation of Fredlund and Xing (1994), a reliable method is proposed to predict the generalized soil-water characteristic curve of fine-grained residual soils using the liquid limit state as the reference.

• Corrosion Science and Technology
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• 제7권3호
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• pp.179-181
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• 2008

With nano-$SiO_2$ and sulphate acid, a kind of colloid electrolyte is synthesized by sol-gel method. It is placed outside the reference electrode as a layer of gel electrolyte so as to decrease the leaching of $Cu^{2+}$ and increase the life of the reference electrode. The performance of the gel electrode in simulating soil solution is measured as follows: the potential of the electrodes ranging from 60 mV to 80 mV (vs. SCE) with potential variation no more than $\pm10mV$, enough resistance to polarization. The $Cu^{2+}$ effusion rate of the reference electrode without gel electrolyte is 3 times that with colloid electrolyte, which means that gel electrolyte can extend the life of the reference electrode significantly.

• Transactions on Control, Automation and Systems Engineering
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• 제3권1호
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• pp.32-42
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• 2001

In this paper, a parameter estimator is developed for the plant model whose structure is represented by the Takagi-Sugeno model. The essential idea behind the on-line estimation is the comparison of the measured stated with the state of an estimation model whose structure is the same as that of the parameterized model. Based on the parameter estimation scheme, and indirect Model Reference Adaptive Fuzzy control(MRAFC) scheme is proposed to provide asymptotic tracking of a reference signal for the systems with uncertain for slowly time-varying parameters. The developed control law and adaptive law guarantee the boundedness of all signals in the closed-loop systems. In addition, the plant state tracks the state of the reference model asymptotically with time for any bounded reference input signal.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.112-121
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• 2017

Edwardsiella tarda is a gram-negative pathogenic bacterium in aquaculture that can cause hemorrhagic septicemia in fish. Many secreted proteins have already been identified as virulent factors of E. tarda. Moreover, since virulent phenotypes are based on the expression regulation of virulent genes, understanding the expression profile of virulent genes is important. A quantitative RT-PCR is one of the preferred methods for determining different gene expressions. However, this requires the selection of a stable reference gene in E. tarda, which has not yet been systematically studied. Accordingly, this study evaluated nine candidate reference genes (recA, uup, rpoB, rho, topA, gyrA, groEL, rpoD, and 16S rRNA) using the Excel-based programs BestKeeper, GeNorm, and NormFinder under different culture conditions. The results showed that 16S rRNA was more stable than the other genes at different culture growth phases. However, at the same culture time, topA was identified as the reference gene under the conditions of different strains, different culture media, and infection, whereas gyrA was identified under the condition of different temperatures. Thus, in experiments, the expression of gapA and fbaA in E. tarda was analyzed by RT-qPCR using 16S rRNA, recA, and uup as the reference genes. The results showed that 16S rRNA was the most suitable reference gene in this analysis, and that using unsuitable reference genes resulted in inaccurate results.

• 한국도서관정보학회지
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• 제36권2호
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• pp.241-262
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• 2005

본 연구에서는 국내 4년제 대학 도서관 중 접속 가능한 236곳의 도서관 홈페이지 분석을 통하여 디지털참고봉사의 제공 및 접근성과 이용 실태에 대해 조사하였다. 진정한 의미의 디지털 참고봉사 즉, 참고질의 응답을 제공하는 대학도서관은 236개 대학 도서관 중 $36.9\%$인 87개 기관으로 우리나라에서 전통적인 참고봉사의 부진이 디지털 환경에서도 나타나는 것으로 파악되었다. 디지털 참고봉사를 적극적으로 활용한다면 전통적인 참고봉사의 부진을 씻고 참고 봉사의 새로운 전환점이 될 수 있을 것으로 판단되며, 분석결과를 바탕으로 현 디지털 참고봉사 시스템의 현황과 문제점을 파악하고 그 발전방향을 제언하였다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.1138-1141
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• 2008

By defining a uniform reference point array corresponding to the 3D voxel array and abandoning voxels whose deviations from their respective reference points exceed a given tolerance, the distribution of voxels within the volumetric 3D image space gets uniform, effects of non-uniform distribution upon the image reconstructing are eased.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2022년도 봄 학술논문 발표대회
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• pp.57-58
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• 2022

Reinforced concrete (RC) structures play a significant role in the construction industries. An embeddable solid-state reference electrode (ESSRE) was used to evaluate the corrosion status of steel rebar in the concrete of various cover thicknesses that exposed to the maritime environment (3.5 % NaCl) in this study. From the open circuit potential measurement (OCP), the passive state, the corrosion uncertainty, and the 90% probability of corrosion state of the steel rebars in the concrete were monitored by ESSRE. From the electrochemical impedance spectroscopy (EIS) method, severe corrosion was observed at the exposure period of 1510, 1847, 2350, and 3020 h for C10, C15, C20, and C30 concrete, respectively. The results confirm that the ESSRE can be useful to identify the corrosion occurrence and severe corrosion of steel rebar embedded in different cover depth concrete structures.