• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.624-630
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• 2008

Terminal restriction fragment length polymorphism (T-RFLP) is a method that has been frequently used to survey the microbial diversity of environmental samples and to monitor changes in microbial communities. T-RFLP is a highly sensitive and reproducible procedure that combines a PCR with a labeled primer, restriction digestion of the amplified DNA, and separation of the terminal restriction fragment (T-RF). The reliable identification of T-RF requires the information of nucleotide sequences as well as the size of T-RF. However, it is difficult to obtain the information of nucleotide sequences because the T-RFs are fragmented and lack a priming site of 3'-end for efficient cloning and sequence analysis. Here, we improved on the T-RFLP method in order to analyze the nucleotide sequences of the distinct T-RFs. The first method is to selectively amplify the portion of T-RF ligated with specific oligonucleotide adapters. In the second method, the termini of T-RFs were tailed with deoxynucleotides using terminal deoxynucleotidyl transferase (TdT) and amplified by a second round of PCR. The major T-RFs generated from reference strains and from T-RFLP profiles of activated sludge samples were efficiently isolated and identified by using two modified T-RFLP methods. These methods are less time consuming and labor-intensive when compared with other methods. The T-RFLP method using TdT has the advantages of being a simple process and having no limit of restriction enzymes. Our results suggest that these methods could be useful tools for the taxonomic interpretation of T-RFs.

• Journal of Periodontal and Implant Science
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• v.45 no.6
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• pp.216-222
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• 2015

Purpose: After extraction, the alveolar bone tends to undergo atrophy in three-dimensions. The amount of alveolar bone loss in the horizontal dimension has been reported to be greater than the amount of bone loss in the vertical dimension, and is most pronounced in the buccal aspect. The aim of this study was to monitor the predictive alveolar bone level following the extraction of anterior teeth seriously involved with advanced chronic periodontitis. Methods: This study included 25 patients with advanced chronic periodontitis, whose maxillary anterior teeth had been extracted due to extensive attachment loss more than one year before the study. Periapical radiographs were analyzed to assess the vertical level of alveolar bone surrounding the edentulous area. An imaginary line connecting the mesial and the distal ends of the alveolar crest facing the adjacent tooth was arbitrarily created. Several representative coordinates were established in the horizontal direction, and the vertical distance from the imaginary line to the alveolar crest was measured at each coordinate for each patient using image analysis software. Regression functions predicting the vertical level of the alveolar bone in the maxillary anterior edentulous area were identified for each patient. Results: The regression functions demonstrated a tendency to converge to parabolic shapes. The predicted maximum distance between the imaginary line and the alveolar bone calculated using the regression function was $1.43{\pm}0.65mm$. No significant differences were found between the expected and actual maximum distances. Likewise, the predicted and actual maximum horizontal distances did not show any significant differences. The distance from the alveolar bone crest to the imaginary lines was not influenced by the mesio-distal spans of the edentulous area. Conclusions: After extraction, the vertical level of the alveolar ridge increased to become closer to the reference line connecting the mesial and distal alveolar crests.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.31 no.6_2
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• pp.567-576
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• 2013

The determination of the geoid in South Korea is a national imperative for the modernization of height datums, specifically the orthometric height and the dynamic height, that are used to monitor hydrological systems and environments with accuracy and easy revision, if necessary. The geometric heights above a reference ellipsoid, routinely obtained by GPS, lead immediately to vertical control with respect to the geoid for hydrological purposes if the geoid height above the ellipsoid is known accurately. The geoid height is determined from gravimetric data, traditionally ground data, but in recent times also from airborne data. This paper illustrates the basic concepts for combining these two types of data and gives a preliminary performance assessment of either set or their combination for the determination of the geoid in South Korea. It is shown that the most critical aspect of the combination is the gravitational effect of the topographic masses above the geoid, which, if not properly taken into account, introduces a significant bias of about 8 mgal in the gravity anomalies, and which can lead to geoid height bias errors of up to 10 cm. It is further confirmed and concluded that achieving better than 5 cm precision in geoid heights from gravimetry remains a challenge that can be surmounted only with the proper combination of terrestrial and airborne data, thus realizing higher data resolution over most of South Korea than currently available solely from the airborne data.

• International journal of advanced smart convergence
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• v.6 no.4
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• pp.9-18
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• 2017

In order to respond to the ever-changing media environments in the era of smart and mobile technology, KBS has introduced and partially applied PIE-TV and PIE-nonTV modes that monitor the average number of viewers among the national population group by means of the sample household extraction method which is a traditional way of rating investigation. This study analyzes the correlation between the number of viewers of premiere, re-air broadcasting, and MPP channel programs and the number of OTT-based VOD viewers in reference to the data extracted from PIE-TV survey results. KBS conducted a survey for 3 months between June and August 2017 to measure the PIE-TV Index, based on which the above-mentioned correlation was analyzed with programs classified to entertainment, drama, and cultural programs. For data analysis, SPSS (Ver. 18.0 for Window, SPSS Inc, Chicago, IL, USA) was utilized. It was assumed that when p<0.05 in the confidence interval of 95%, statistically significance would be secured. Among the 30 subjects in the simple correlation analysis, the parameter was determined by the Person correlation coefficient and the non-parameter by the Spearman correlation coefficient. Analysis results are as below: (1) As the number of viewers of premier entertainment, drama, and cultural programs was larger, the number of VOD viewers was larger accordingly. (2) As for entertainment and drama programs, as the number of re-air broadcasting viewers was larger, the number of VOD viewers decreased accordingly. (2) As for entertainment and drama programs, as the number of MPP viewers was larger, the number of VOD viewers decreased accordingly. It is expected that this statistical data can be utilized for strategic planning of MPP channel lineups including terrestrial TV broadcasting, cable TV, etc.

• Korean Journal of Remote Sensing
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• v.29 no.5
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• pp.537-544
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• 2013

Baton Peninsula, where Sejong station is located, mainly covered with snow and vegetation. Because this area is sensitive to climate change, monitoring of surface variation is important to understand climate change on the polar region. Due to the inaccessibility, the remote sensing is useful to continuously monitor the area. The objectives of this research are 1) map classification of land-cover types in the Barton Peninsular around King Sejong station and 2) grasp distribution of vegetation species in classified area. A KOMPSAT-2 multispectral satellite image was used to classify land-cover types and vegetation species. We performed classification with hierarchical procedure using KOMPSAT-2 satellite image and ground reference data, and the result is evaluated for accuracy as well. As the results, vegetation and non-vegetation were clearly classified although species shown lower accuracies within vegetation class.

• Journal of Intelligence and Information Systems
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• v.9 no.1
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• pp.53-69
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• 2003

Many process variables in modem manufacturing processes have influence on quality of products with complicated relationships. Therefore, it is necessary to control multiple quality variables in order to monitor abnormal signals in the processes. This study proposes an integrated procedure of self-organizing map (SOM) neural network and case-based reasoning (CBR) for multivariate process control. SOM generates patterns of quality variables. The patterns are compared with the reference patterns in order to decide whether their states are normal or abnormal using the goodness-of-fitness test. For validation, it generates artificial datasets consisting of six patterns, normal and abnormal patterns. Experimental results show that the abnormal patterns can be detected effectively. This study also shows that the CBR procedure enables to keep Type 2 error at very low level and reduce Type 1 error gradually, and then the proposed method can be a solution fur multivariate process control.

• The Journal of Society for e-Business Studies
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• v.9 no.1
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• pp.39-59
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• 2004

E-business automates overall activities in a company and allows distributed systems to communicate their information. The automation and information sharing improves its productivity and responsiveness. B2B workflow systems progress their business processes electronically and help the companies to monitor their execution and administrate the processes. This research analyzes the technologies and standards for B2B workflow integration, and proposes three approaches to implement effectively workflow integration. We analyze several existing workflow standards and business process definition languages, and compare their structures and features. And we examine the messaging technologies and architectures to implement business integration. Finally we extract a reference model for B2B workflow integration, and propose three types of workflow integration and implementation designs. This research helps companies to understand the workflow standards and the messaging technologies for B2B workflow integration, and to implement workflow integration systems that are suitable for their e-business environments.

• Journal of Astronomy and Space Sciences
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• v.24 no.4
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• pp.269-274
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• 2007

We established a regional ionospheric model for investigating ionospheric TEC (Total Electron Contents) variations over the Korean Peninsula during major geomagnetic storms. In order to monitor the ionospheric TEC variations, we used nine permanent GPS reference stations uniformly distributed in South Korea operated by the Korea Astronomy and Space Science Institute (KASI). The cubic spline smoothing (CSS) interpolation method was used to analyze the characteristics of the ionospheric TEC variations. It has been found that variations of TEC over the Korean Peninsula increase when a major geomagnetic storm occurred on November 20, 2003. The TEC has increased about one and a half of those averaged quite days at the specific time during a geomagnetic storm. It has been indicated that the KASI GPS-derived TEC has a correlation with the geomagnetic storm indices (eq. Kp and Dst indices).

• The Journal of Korean Society for Radiation Therapy
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• v.11 no.1
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• pp.6-10
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• 1999

The field size can be beam output, therefore MonitorUnit can be varied due to field size dependence The purpose of this study is to evaluate and compare the dose variation according to exchange of collimator The measurements were perfomed with Wellhofer dosimetry system(water phantom. ion chamber. electrometer. system controller. build up cap. etc)and two types of linear accerlerator (Mevatron KD, MevatronMX) Scatter can be affected to field size dependence and scatter correction is separated into collimator and phantom components, scatter components can affect by exchanging of collimator Measurements of collimator scatter factor(Sc) was done in air with build up cap. 1)Square field (5cm2 to 40cm2) was measured 2)and then keeping the upper jaw constant at loom and varing lower jaw from 5cm to 40cm, 3)keeping the lower jaw constant at 10cm and varing upper jaw from 5cm to 40cm Measurements of total scatter factor(Scp) was done in water at Dmax as the procedure of collimator scatter factor measurements in water Dmax The total scatter factors were obtained to the following equation(Sp=Scp/Sc) The measured data is normalized to the data of reference field size($10{\times}10$), rectangular field is inverted to equivalent field to compare three field size data As the collimator setting is varied, the output was changed In conclusion, the error was obtained small but it must be eliminated if we intend to reach the common stated goal of $5\%$ overall uncertainty in dose determination

• Journal of Biomedical Engineering Research
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• v.35 no.6
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• pp.177-184
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• 2014

Continuous monitoring of heart rates and respiratory rates for newborns or infants is very important since the abnormal breathing and heart problems can threaten the life of newborns or infants. A noncontact baby monitoring system based on a Doppler radar and an air mattress was designed. The Doppler radar was used to acquire respiratory signals and the air mattress was employed to obtain heart rates. The performance of the designed system was evaluated using a commercialized infant simulator ($Simbaby^{TM}$) and a respiration belt transducer was used to measure respiration rates as a reference. Results for respiratory rates revealed that the correlation coefficients between I-and Q-channel and the respiration belt were 0.84 and 0.91 and the mean ${\pm}$ standard deviations of errors between them were $1.66{\pm}1.92$ (bpm) and $0.88{\pm}1.65$ (bpm). Heart rates showed that the correlation coefficient between air mattress and set value of the simulator was 0.73 and the mean ${\pm}$ standard deviation of errors between them was $1.09{\pm}3.45$ (bpm). These results indicate that the designed system holds the potential as an effective monitoring tool for continuous monitoring heart rates and respiratory rates of newborns or infants.