• Journal of Animal Science and Technology
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• v.57 no.6
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• pp.21.1-21.8
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• 2015

The digestibility of different vegetable protein sources were investigated and the effects of supplementing canola meal (CM) as partial inclusions were studied in growing pigs, to determine the performance parameters and its economic importance. In Exp. 1, four pigs (average initial $BW=15.4{\pm}0.35kg$, 5 weeks of age) fitted with simple T-cannula at terminal ileum, were fed four diets following repeated $4{\times}4$ Latin square design having adoption period of 7 days. Diet 1 was Nitrogen free diet containing corn starch. Diets 2, 3, and 4 were the basal diet supplemented with soybean meal (SBM), rapeseed meal (RSM), and domestic CM respectively. The AID of crude protein was decrease in RSM in comparison to SBM supplementation. The AID of Dietary indispensable amino acids (DIAA) such as Lys, Meth, Pha, and dispensable amino acid Ala, Pro, Asp were decreased (P < 0.05) in RSM supplemented diets. The SID of DIAA does not differ but the SID of Asp was higher (P < 0.05) in RSM and CM diets while SID of Pro was lower (P < 0.05) in RSM in comparison to SBM supplemented diets. In Exp. 2, 192 growing pigs (average initial BW $24.76{\pm}2.55kg$) were randomly allotted to four dietary treatments with increasing levels of CM i.e. 0, 3.75, 7.50, and 11.25 % respectively. Diets were fed in meal form for 35 days. Increasing CM levels in diets had no effects (P > 0.05) on growth performance and apparent total tract digestibility (ATTD) of nutrients and energy. Total weight gain, total feed intake, and feed cost per kg weight gain were not affected by increasing levels of CM in diets but total feed cost (TFC) per pigs was linearly reduced (26.463 to 25.674; P < 0.05). Broadly, the AID, and SID of amino acid was reduced in RSM but was not effected in CM in comparison to SBM supplemented pigs. Moreover, increasing levels of CM in pigs diet had no effect on the ATTD and performance but TFC per pig was reduced. Thus CM inclusion of up to 11.25 % in diets can be used for reducing the production cost in growing pigs without any negative effect.

• Food Science and Preservation
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• v.19 no.6
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• pp.866-872
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• 2012

This study was conducted to investigate the changes in physicochemical properties of Spergularia marina Griseb by blanching. The proximate composition, free sugar, free amiao acid, organic acid, vitamin and mineral composition of raw and blanching Spergularia marina Griseb were compared. After blanching treatment, the moisture and carbohydrate contents of Spergularia marina Griseb increased, but the crude protein, crude fat and crude ash contents decreased. Total free sugar content decreased by 62.50% compared with raw Spergularia marina Griseb after blanching, and the reduction of galactose was the largest in free sugars. The raw and blanched Spergularia marina Griseb contained all the essential amino acids, except tryptophan. The total amino acid and essential amino acid contents were reduced by blanching, but the essential amino acid ratios of the raw and blanched Spergularia marina Griseb were similar. The amounts of all the detected organic acids were reduced by blanching, except acetic acid. Spergularia marina Griseb was found to contain high levels of vitamin C, vitamin E, and minerals, but these were reduced by blanching. These results showed that most of the nutrients contained by Spergularia marina Griseb are decrease by blanching. Therefore, the blanching conditions that can reduce nutrient loss of Spergularia marina Griseb must be established.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.3
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• pp.183-189
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• 2021

We checked the emergence rate and early growth characteristics of young rice plants according to the germination length and seed damage ratio in direct seeding. The emergence rate was reduced according to the longer germination length and higher seed damage ratio. The emergence rate was further reduced under high-temperature conditions. The leaf number did not show a significant difference according to germination length and seed damage ratio. However, shoot and root dry weights were reduced according to the germination length and seed damage ratio. The root dry weight was highly correlated with the shoot dry weight. Based on these results, we concluded that a longer germination length and seed damage could induce the loss of nutrients and affect poor root growth, consequently inducing poor emergence and growth in the early growth stage of rice seeds. Therefore, in rice direct seeding, seed conditions of less than 1 mm of germination length should be checked for a sufficiently high standing rate and adequate growth characteristics after seeding.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.445-456
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• 2001

In the 1990s, Cochlodinium polykikoides red tide has been annually occurred in the southern coast of Korea and caused the mass damage to the fisheries with a huge amount of economic loss. The present study was done to establish the biological foundation for the elucidation of the mechanism of C. polykikoiaes red tide. The growth response of C. polykikoides to physico-chemical factors such as temperature, salinity, pH, and light intensity were examined using axenic cultures to evaluate the relative importance of these factors on the dynamics of natural populations, It was found that the highest growth conditions were $25^{\circ}C,\;40\%_{\circ}$, pH 7.5, and 7,500 lux, respectively. The tolerable salinity range of growth was relatively wide at an optimum temperature and was reduced to a much narrower range at a sub-optimum temperature. These findings indicate that C. polykikoides is an eurythermal and euryhaline organism. The organism demanded higher light intensity and oceanic pH narrow in its growth. C. polykikoides utilize inorganic nutrients, such as nitrate and ammonium as N, and phosphate as P. The nutritional requirements of C. polykikoides were $40{\mu}M$ for nitrate, $50{\mu}M$ for ammonium, and $5{\mu}M$ for phosphate. The half saturation constant (Ks) for growth was $2.10{\mu}M$ for nitrate, $1.03{\mu}M$ for ammonium, and $0.57{\mu}M$ for phosphate. These values were comparatively smaller than those of other dinoflagellates reported previously. We confirmed that the organism is characterized as an eutrophic species. However, ammonium Ks value is smaller than that of other eutrophic species, This result indicates that C. polykikoides red tide may outbreak in the waters which eutrophication is in progress rather than eutrophicated waters. C. polykikoides preferred ammonium better than nitrate as a nitrogen source when in a growth stage, Therefore, our results indicate that ammonium is more important nutrient on the growth of the organism in comparison with other inorganic nutrients and C. polykikoides red tide is related with the increased ammonium concentration in the coastal waters.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.5
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• pp.523-530
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• 2020

Based on the results of harmful algal blooms (HABs) monitoring by the National Institute of Fisheries Science and local governments, the effects of changes in the marine environment on HABs are described. Since the beginning of HABs monitoring in 1972, they continued to increase from the 1980s to the 1990s. After the largest number of HAB incidents (109) in 1998; the trend declined until the 2010s. Most HABs in the 1970s were caused by diatoms. In the 1980s, coastal dinoflagellates caused HABs; Cochlodinium polykrikoides blooms have been occurring continuously since 1993. There are three HAB species that cause damage to fisheries in Korea. The high-density bloom of Karenia mikimotoi caused mass mortality in shellfish in Jinhae Bay in 1981. Karenia sp. blooms occurring around Tongyeong in 1992 killed aquaculture fish. Since the occurrence of the largest fisheries damage of KRW 76.6 billion in 1995 caused by C. polykrikoides blooms, they have been occurring continuously. The concentration of nutrients in coastal waters was the highest in the 1980s and has declined since the mid-1990s. This reduction in nutrient concentration is a good explanation for the decreasing number of HABs. Since 2016, a summer high water temperature of 30℃ or more has appeared, and the range and scale of C. polykrikoides blooms have been greatly reduced. In 2016, K. mikimotoi blooms occurred around Wando, Jangheung and Goheung and small scale blooms of C. polykrikoides occurred around Yeosu. There were no C. polykrikoides blooms in 2017; however, Alexandrium affine blooms occurred from Yeosu to Tongyeong. There was a small-scale blooms of C. polykrikoides in 2018 compared to those in the previous years. Our results show that reduction in nutrients and the high water temperature owing to climate change are a good explanation for variation in HABs in Korean coastal waters.

• Korean Journal of Environmental Biology
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• v.19 no.1
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• pp.7-17
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• 2001

Growth optima and tolerance ranges of marine algae species nay vary greatly during the developmental stage. The establishment stage is clearly of great importance for each generation, affecting the performance of the adult population. The present study was aimed to determine the effect of various concentrations of inorganic nutrients and heavy metals on germination and germlings of Ulva pertusa Kjellman (Chlorophyta). Percent germination increased rapidly as irradiance level increased, reaching the maximum at 100 ${\mu}$mol m$\^$-2/s$\^$-1/ in both east and west seawater. Percent germination in the east seawater was higher than that in the west seawater at irradiances lower than 30 ${\mu}$mol m$\^$-2/s$\^$-1/, and there was no difference in percent germination between the two different seawaters at irradiances higher than 60 ${\mu}$mol m$\^$-2/s$\^$-1/. Germling growth increased in the both cases with increasing irradiances but no growth was found in the dark. Overall germling area was larger in the east seawater than in the west seawater. Number of cell increased with increasing irradiance and became light-saturated at 100 ${\mu}$mol m$\^$-2/s$\^$-1/ in the both cases. Germlings grown in the east seawater had more cells than those cultivated in the west seawater at irradiances lower than 60 ${\mu}$mol m$\^$-2/s$\^$-1/. In various combinations of nitrate and phosphate, percent germination increased with increasing nitrate concentrations irrespective of phosphate concentrations. At 3 days after inoculation, nitrate concentration of 2.5 ppm with all phosphate concentrations promoted germination. Area and number of cell of germlings increased with increasing nitrate concentrations in all phosphate concentrations. In various combinations of copper and lead, germination was delayed as copper concentrations increased in all lead concentrations. Percent germination was severely reduced at 1 ppm Cu$\^$2+/. Area and number of cell of germlings decreased with increasing copper concentrations in all lead concentrations. The highest copper concentration (1 ppm) clearly suppressed the germling growth in U. pertusa.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.1
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• pp.45-52
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• 2020

This study analyzed the components contained in the pine needles of first and second-year-olds to analyze the factors that the in vivo content of inorganic elements affects the sex determination of pine trees. In response, the plan for pine tree plant and maintenance was intended to be presented in consideration of the reproductive environment and physiological characteristics. The results are as follows. First, last year, when there were many encyclopedias, the analyzed N(%) content was found to be high. The nitrogen content of the previous year's soil was found to affect the production of the spheres the following year. This is believed to be possible to reduce the rate of Pine pollen produced in the new plant in the following year through a dispute over quality consumption in the spring of the previous year. Second, the weapons elements involved in the Pine cones and the generation of the Pine pollen in the new plant appeared to be P(%), K(%), Ca(%), and Fe(%). However, the nutrients from the previous year's leaves of Ca(%) and Fe(%) were found to have a low influence on the sex determination of first-year pine trees. Because Ca(%) and Fe(%) are not able to move nutrients accumulated in aging organs due to the nature of the components, feeding nutrients in the fall when the growth of the previous year's branches is reduced is expected to affect oral generation. Third, pine trees are extremely positive and Pine pollen is related to the time of the northeast wind. Therefore, it is believed that it would be good to be located in the northern direction, where the sunlight is good, in an outdoor space. In addition, it is important to use vaginal consumer products in spring and carry out a quarrel involving Mg and Fe during fall to reduce the effect of the Pine pollen, which is an outdoor plant. This is an important part of maintaining and managing pine trees in outdoor spaces as well as the sex determination of pine trees. This study suggested that plant planning, which derives the correlation between pine inorganic element content on sexual determination and takes into account the physiological characteristics of pine trees, is an important issue in the creation of outdoor space. Follow-up research on other factors affecting pine tree sex determination is expected.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.3
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• pp.231-238
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• 1986

This field experiment was undertaken to assess the effects of three levels of inclination ($10^{\circ}$, $20^{\circ}$ and $30^{\circ}$) and four rates of $N-P_2O_5-K_2O$ (0-0-0, 14-10-10, 28-25-25, and 42-40-40 kg/10a) on the hilly pasture concerning to establishment, forage yield and quality, and botanical composition in the mixed grass-clover sward set up on a new reclaimed woodland by an oversowing method. This part was concerned with the concentration and mutual balance of mineral nutrients in relation to grass tetany hazard. The results obtained were summarized as follows: 1. Concentration of exchangeable Mg, relative proportions of Mg to CEC, K, and Ca in soil were considerably below the critical level for good forage growth and prevention of grass tetany. These properties were rather worsened by increasing the inclination grade and the NPK rate. 2. Compared with the critical level for the likelihood of tetany (Mg < 0.2%, K > 2.5%, and K/(Ca+Mg) equivalent ratio > 2.2 in forages in DM basis), mean concentration of Mg in mixed forages turned out to range from 0.09 to 0.14%. 2.6% of K concentration and 2.5 of K/(Ca+Mg) equivalent ratio were shown to be at a normal NPK rate. The likelihood of grass tetany was generally enhanced by increasing the inclination grade and the NPK rate. 3. The increasing of the inclination grade and NPK rate rather aggravated the low Mg concentration and unbalanced mutual ratios of mineral nutrients, from which resulted the grass tetany hazard and the depression of forage growth. To prevent these two resulted factors, Mg fertilization on grassland and/or extra Mg supplement to dairy cattle needed to be carried out. 4. The Ca/P ratios in the mixed forages ranging from 1.0 to 2.0 were reduced by the increased rate of NPK, whereas the difference due to inclination grade was yet to be recognized.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.367-374
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• 2000

The composition of dissolved gases and nutrients in a liquid medium were determined for establishment of the optimum conditions for in vitro culture of Helicobacter pylori. A microaerobic condition facored by the organism was prepared by adjusting the partial pressure of the gas, agitation speed, and viscosity of the medium. The gaseous concentrations were controlled by utilizing CampyPak Plus that reduced oxygen while augmenting carbon dioxide. Agitation of the broth facilitated the oxygen transfer to the cells, yet inhibited the growth at high rates. An increase of viscosity in the medium repressed the culture although this variable was relatively insignificant. The chemical constituents of the liquid broth were examined to establish an economic model for H. pylori cultivation. The microbe required a neutral pH for optimum growth, and yet was also able to proliferate in an acidic condition, presumably by releasing the acidity-modulating enzyme, urease. Cyclodextrin and casamino acid were investigated as growth enhancers in place of serum, while yeast extract unexpectedly inhibited the cells. A low concentration of glucose, the unique carbon source for the organism, increased the cell density, yet high concentrations resulted in an adverse effect. Under optimally dissolved gas conditions, the cell concentration in brucella broth supplemented with serum substitutes and glucose reached $1.6{\times}10^8$ viable cells/ml which was approximately 50% higher than that obtained in the liquid medium added with only cyclodextrin or serum.

• Korean Journal of Soil Science and Fertilizer
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• v.14 no.3
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• pp.130-136
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• 1981

Rice hull was reduced to ash by carbonization grades to illcuidate alkalinity increase and extract of inorganic nutrients in the rice hull charcoal. Alkaline reaction of water extraction was neutral at less carbonized charcoal, but much carbonized ash from 65% weight loss reached over 10 of pH value, also origin shape of rice hull was maintained until near 65% carbonized grade. Therefore, physical properties sustained good condition for seedling bed. The more charcoal carbonized to ash, the pH value and concentration of inorganic nutrient in their extracts were increased gradually. Nitric acid concentrations for neutralizing extract from charcoal were stronger in proportion to the carbonized grade but 0.1 N nitric acid solution was very reasonable to neutralize the 65% carbonized charcoal for mixing with heavy texture acidy soil(pH 5.3) of uncultivated deep horizon to transplant the tobacco seedlings. Volume ratio mixing for seedling bed is adequate at five of ash to one of acid solution. Neutralization with nitric acid solution also accelerated extraction of the inorganic nutrient in rice hull ash. Tobacco seedlings grown on bed mixed with neutralized rice hull charcoal and soil had shown better results on the agronomic measurement than alkaline ash bed, and phosphorus and cations were uptaken more amounts.