• Journal of Animal Science and Technology
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• 제63권5호
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• pp.998-1007
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• 2021

Brine mineral water (BMW) is groundwater near the deep sea, and the mineral component of the BMW is different from the water of the deep sea because the components of BMW are derived from the unique geographical features surrounding it. Recently, BMW has attracted attention due to the unique health-related minerals it possesses; however, the influence of BMW on physiological function has not yet been determined in domestic animals. Therefore, this experiment investigated the influence of BMW on the growth performance, water intake, blood properties, and immunoglobulin (Ig) levels of serum in growing-finishing pigs. A total of 64 pig barrows (Landrace × Yorkshire × Duroc) with an average initial weight of 40.56 ± 0.17 kg were used in the experiment, and 0%, 2%, 3%, and 5% samples of BMW diluted with freshwater were provided to experimental animals during the 56 days. We found that the gain/feed ratio in the 3% BMW group was significantly higher than that in the 5% BMW group of growing-finishing pigs (p < 0.05). The water intake was significantly increased in the 5% BMW group compared with the other groups (p < 0.05) of growing-finishing pigs. Additionally, the concentrations of red blood cells (RBCs), hemoglobin (HGB), and hematocrit (HCT) were significantly higher in the 3% BMW group than in the control group. The level of high-density lipoprotein cholesterol was higher in the 3% BMW group than in the 5% BMW group (p < 0.05). Furthermore, IgG and IgM levels in the serum were significantly higher in the 2% and 3% BMW groups than in the control group (p < 0.05). These results suggest that a dilution of 3% BMW in the intake water could improve the levels of RBCs and serum Igs in growing-finishing pigs.

• Restorative Dentistry and Endodontics
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• 제47권4호
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• pp.42.1-42.11
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• 2022

Objective: This study investigated the effects of various concentrations of sodium hypochlorite (NaOCl) on human whole-blood clotting kinetics, the structure of the blood clots formed, and transforming growth factor (TGF)-β1 release. Materials and Methods: Human whole blood was collected from 5 healthy volunteers and divided into 4 groups: CG (control, 0.5 mL of blood), BN_0.5 (0.5 mL of blood with 0.5 mL of 0.5% NaOCl), BN₃ (0.5 mL of blood with 0.5 mL of 3% NaOCl), and BN_5.25 (0.5 mL of blood with 0.5 mL of 5.25% NaOCl). The effects of NaOCl on clotting kinetics, structure of fibrin and cells, and release of TGF-β1 were assessed using thromboelastography (TEG), scanning electron microscopy (SEM), and enzyme-linked immunosobent assay, respectively. Statistical analysis was conducted using the Kruskal Wallis and Mann-Whitney U tests, followed by the post hoc Dunn test. A p value < 0.05 indicated statistical significance. Results: The blood samples in BN_0.5 and BN₃ did not clot, whereas the TEG of BN_5.25 showed altered clot formation. Samples from the CG and BN₃ groups could only be processed with SEM, which showed that the latter lacked fibrin formation and branching of fibers, as well as clumping of red blood cells with surface roughening and distortion. TGF-β1 release was significantly highest in BN₃ when all groups were compared to CG (p < 0.05). Conclusions: Each concentration of NaOCl affected the release of TGF-β1 from blood clots and altered the clotting mechanism of blood by affecting clotting kinetics and cell structure.

• 한국수정란이식학회지
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• 제25권4호
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• pp.287-290
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• 2010

On January 6, 2010, two months earlier than normal breeding season, a red fox vixen was implanted with synthetic GnRH analogue, Deslorelin. Blood was sampled every 2~3 days from the day of implant to identifying spermatozoa on stains of epithelial cells. Estradiol and progesterone were examined. Even though the vixen was in non-breeding season, she was mated by a male fox. Pregnancy was confirmed by canine pregnancy detection kit that detect relaxin released from placenta. Four healthy pups were born on March 9, 2010. This is the first report showing synthetic GnRH can activate ovarian function and lead to fertile estrus of red fox in non-breeding season.

• 한국임상수의학회지
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• 제16권1호
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• pp.214-217
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• 1999

An 8-month-old male orangutan (Pongo pygmaeus) housed in Everland Zoo-logical Gardens died after having 10 day history of depression, anorexia and acute respiratory distress. On necropsy, the lung failed to collapse and showed diffuse red consolidation. Microscopically, the alveoli were filled with large amounts of red blood cells, fibrin and varing number of hemosiderin-laden macrophages. The alveolar septa were thickened due to infiltrated mononuclear cells and congestion and are occasionally necrotic. Escherichia coli was isolated from the lung, spleen and kidney. The result of antibiotic sensitivity test of the isolated organism was also described.

• 한국자기학회지
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• 제27권3호
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• pp.92-97
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• 2017

펄스자기장을 인체에 자극했을 때 몸 안에 있는 이온성 전해물질에 의해 유도전류가 발생하며, 최근에는 이를 활용하여 자기장 치료기술이 새롭게 연구 개발되고 있다. 본 연구에서는 강한 펄스자기장을 자극했을 때, 여러 층으로 응집되어 붙어있는 적혈구 연전형성의 변화를 동역학적으로 조사 분석하였다. 적혈구의 연전형성은 여러 가지 원인에 의해 발생하는데 물리학적으로 보면 적혈구 중심에 있는 $Fe^{+3}$ 이온에 의한 전기장 발생과 혈액 내에 녹아있는 음이온의 상호작용이 원인으로 고려된다. 본 연구에서 사용된 펄스자기장의 세기와 시간은 0.27 Tesla, 0.102 msec이고 펄스 반복시간은 1초이다. 인체에 가한 펄스자기장의 자극시간은 5분에서 10분, 15분, 20분까지 증가시켰고, 이에 따라 적혈구의 연전상태 변화를 동영상으로 조사하였다. 펄스자기장을 10분 동안 가했을 때 적혈구의 연전상태는 매우 호전되어 대체로 개별로 분리되었으며 slide glass 위에서 이동속도 역시 가장 활발하게 움직여서 $8{\times}10^{-4}m/sec$로 증가하였다. 그러나 20분까지 자기장 자극을 증가시키면 오히려 적혈구의 연전상태가 증가하고 활동성이 떨어지는 것을 확인하였다. 이에 대한 해석은 좀 더 체계적인 연구와 분석이 필요해 보인다.

• ALGAE
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• 제31권3호
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• pp.205-217
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• 2016

Freshwater red tides due to dinoflagellates have caused spectacular and regular “summer reddening” in recent years in Baishihai Lake, a temperate, meromictic, meso- or oligotrophic, high-altitude, landslide-dammed, deep lake located at the eastern edge of Tibetan Plateau in China. Based on morphological and molecular analyses, the causative organism has been identified as a new woloszynskioid dinoflagellate, Tovellia diexiensis Q. Zhang et G. X. Liu sp. nov. The vegetative cells are 20-32 μm long and 16-24 μm wide. They have a hemispherical episome and a broadly rounded hyposome with a short characteristic antapical spine. Usually cells are bright red due to the presence of numerous red-pigmented bodies, which often masked the yellowish green discoid chloroplasts. The amphiesma of motile cells comprise mainly quadrilateral, pentagonal or hexagonal thin plates, arranged in 4-5 latitudinal series on the episome, 1 in the cingulum and 4 on the hyposome. Molecular phylogenies based on small subunit ribosomal DNA and large subunit ribosomal DNA (LSU) indicate T. diexiensis from Baishihai Lake to belong to the family Tovelliaceae, which was monophyletic in our LSU phylogenies. During the bloom-forming period in 2005, cell density of T. diexiensis reached 9.15 × 10⁵ cells L⁻¹. Astaxanthin and its diester were found to be the major pigments in T. diexiensis, resulting in a characteristic blood-red color of the water in Baishihai Lake.

• 한국발생생물학회지:발생과생식
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• 제19권4호
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• pp.209-215
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• 2015

Red spotted grouper, Epinephelus akaara is a popular aquaculture species in many Asian countries. This species is a protogynous hermaphrodite that first differentiates into female and changes to male later. Due to this reproductive characteristic, stable supply of male and female gametes is a key to the success of seed production in this species. Thus, understanding early gonadal differentiation is required to develop effective sex control techniques. Red spotted grouper were reared in indoor tanks and sampled every 5 days from 40 days post-hatch (DPH) to 130 DPH. Changes of gonadal tissues were examined and analyzed by means of histology. A pair of gonadal primordium has already existed underneath the kidney in the posterior part of the body cavity at 38 DPH when this study began. Gonadal primordia of 38, 40 DPH consisted of germ cells surrounded by a few somatic cells. The blood vessel was observed in the gonadal primordium at 45 DPH. The number of somatic cells and size of gonadal primordium increased age-dependently up to 60 DPH. Formation of ovarian cavity was obvious by two protuberant aggregations of somatic cells at 65 DPH. Completed ovarian cavity and oogonia were first observed in the gonad of one fish sample at 105 DPH. Based on these histological observations, it can be suggested that induction of primary male differentiation could be more successfully applied at around 60 DPH in this species.

• Journal of Animal Science and Technology
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• 제55권4호
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• pp.289-293
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• 2013

The objective of this study was to assess the effects of dietary carbohydrases on growth performance, nutrient digestibility and blood characteristics in finishing pigs. A total of 90 pigs [(Landrace ${\times}$ Yorkshire) ${\times}$ Duroc] (initial BW = $56.15{\pm}1.26kg$) were used for a 35 d feeding trial. The dietary treatments included: 1) CON (control diet), 2) MIX (CON + mixture with ${\alpha}$-galactosidase and ${\beta}$-mannanase 0.05%) and 3) MAN (CON + ${\beta}$-mannanase 0.05%). There were six replications per treatment with five pigs per pen. The average daily gain (ADG) in MIX was higher than in CON (p<0.05). No significant differences were noted in the average daily feed intake (ADFI) and feed efficiency (G:F) among dietary treatments (p>0.05). Apparent total tract digestibility (ATTD) of dry matter (DM) and energy (E) in MIX increased (p<0.05) relative to CON and MAN. The ATTD of nitrogen (N) in MIX was higher (p<0.05) than in CON. No differences in red blood cells (RBC), white blood cells (WBC), lymphocytes and IgG concentrations were observed among dietary treatments (p>0.05). In conclusion, the addition of the mixture of carbohydrases (${\alpha}$-galactosidase and ${\beta}$-mannanase 0.05%) increased ADG and nutrient digestibility in finishing pigs.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.1889-1891
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• 2004

The differential white blood cell count plays an important role in the diagnosis of different diseases. It is a tedious task to count these classes of cell manually. An automatic counter using computer vision helps to perform this medical test rapidly and accurately. Most commercial-available automatic white blood cell analysis composed mainly 3 steps including segmentation, feature extraction and classification. In this paper we concentrate on the first step in automatic white-blood-cell analysis by proposing a segmentation scheme that utilizes a benefit of active contour. Specifically, the binary image is obtained by thresolding of the input blood smear image. The initial shape of active is then placed roughly inside the white blood cell and allowed to grow to fit the shape of individual white blood cell. The white blood cell is then separated using the extracted contour. The force that drives the active contour is the combination of gradient vector flow force and balloon force. Our purposed technique can handle very promising to separate the remaining red blood cells.

• Journal of Ginseng Research
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• 제34권2호
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• pp.138-144
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• 2010

Ginseng (Panax ginseng C.A. Meyer) has been shown to have anti-stress effects in animal studies. However, most studies have only managed to detect altered levels of biomarkers or enzymes in blood or tissue, and the actual molecular mechanisms by which ginseng exerts these effects remain unknown. In this study, the anti-oxidative effect of Korean red ginseng (KRG) was examined in human SK-N-SH neuroblastoma cells. Incubation of SK-N-SH cells with the oxidative stressor hydrogen peroxide resulted in significant induction of cell death. In contrast, pre-treatment of cells with KRG decreased cell death significantly. To elucidate underlying mechanisms by which KRG inhibited cell death, the expression of apoptosis-related proteins was examined by Western blot analysis. KRG pre-treatment decreased the expression of the pro-apoptotic gene caspase-3, whereas it increased expression of the anti-apoptotic gene Bcl-2. Consistent with this, immunoblot analysis showed that pre-treatment of the SK-N-SH cells with KRG inhibited expression of the pro-inflammatory gene cyclooxygenase 2 (COX-2). RT-PCR analysis revealed that the repression of COX-2 expression by KRG pre-treatment occurred at the mRNA level. Taken together, our data indicate that KRG can protect against oxidative stress-induced neuronal cell death by repressing genes that mediate apoptosis and inflammation.