• BMB Reports
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• v.28 no.6
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• pp.509-514
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• 1995

A putative secondary structure of the mRNA for the human hepatitis B virus (HBV) X gene is proposed based on not only chemical and enzymatic determination of its single- and double-stranded regions but also selection by the computer program MFOLD for energy minimum conformation under the constraints that the experimentally determined nucleotides were forced or prohibited to base pair. An RNA of 536 nucleotides including the 461-nucleotide HBV X mRNA sequence was synthesized in vitro by the phage T7 RNA polymerase transcription. The thermally renatured transcripts were subjected to chemical modifications with dimethylsulfate and kethoxal and enzymatic hydrolysis with single strand-specific RNase T1 and double strand-specific RNase V1, separately. The sites of modification and cleavage were detected by reverse transcriptase extension of 4 different primers. Many nucleotides could be assigned with high confidence, twenty in double-stranded and thirty-seven in Single-stranded regions. These nucleotides were forced and prohibited, respectively, to base pair in running the recursive RNA folding program MFOLD. The results suggest that 6 different regions (5 within X mRNA) of 14~23 nucleotides are Single-stranded. This putative structure provides a good working model and suggests potential target sites for antisense and ribozyme inhibitors and hybridization probes for the HBV X mRNA.

• The Journal of Korean Association of Computer Education
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• v.21 no.6
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• pp.39-47
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• 2018

This paper dealt with investigating the number of minimal spanning ladders originated from ladder game and their properties as well as the related computational thinking aspects. The author modified the filtering techniques to enhance Mathematica project where a new type of graph was generated based on the algorithm using a generator of firstly found minimal spanning graph by repeatedly applying independent ladder operator to a subsequence of ladder sequence. The newly produced YC graphs had recursive and hierarchical graph structures and showed the properties of edge-symmetric. As the computational complexity increased the author divided the whole search space into the each floor of the newly generated minimal spanning graphs for the (5, 10) YC graph and the higher (6, 15) YC graph. It turned out that the computational thinking capabilities such as data visualization, abstraction, and parallel computing with Mathematica contributed to enumerating the new YC graphs in order to investigate their structures and properties.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.8
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• pp.555-563
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• 2020

Foward-swept wings are known to possess superior aerodynamic performance compared to the conventional straight wings. However major concerns regarding forward-swept wings include divergence at lower airspeeds which require careful consideration at the design stage. As an endeavor to overcome such drawbacks, aeroelastic tailoring is attempted. In order to find an optimal ply sequence, recursive aeroelastic analyses is conducted and one-dimensional beam analysis coupled with simple aerodynamics is used for the improved computational efficiency and modelling convenience. The analysis used in this paper, DYMORE and analytic formula, both use one-dimensional beam model for the structure. Cross-sectional analysis for multi-cell NACA0015 airfoil section is conducted using VABS and oblique function is used for the sweep angle. Throughout the present aeroelastic tailoring, the maximum divergence speed of 290.2m/s is achieved which is increased by approximately 43% than that for the conventional ply configuration.