• Korean Chemical Engineering Research
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• v.59 no.2
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• pp.276-280
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• 2021

The oxygen transfer rate at the liquid surface of the reciprocally shaking vessel was studied. The required power of the reciprocally shaking vessel was not proportional to the shaking frequency, unlike the rotational shaking vessel, and the liquid level suddenly fluctuated greatly at a certain frequency as the flow pattern in the vessel was a left and right wave flow different from that of the rotational shaking that has a rotational flow. The effect of the shaking frequency on the required power in the reciprocally shaking vessel was very complex, such as less power required than the rotational shaking vessel when the shaking frequency is more than 3 s-1, but the required power for the range of the generated rotational flow in the reciprocally shaking vessel could be correlated with the equation that was reported for the rotational shaking vessel. The kLa (mass transfer capacity coefficient) in the reciprocally shaking vessel also increased in a complex pattern because the required power for shaking was not consumed in a simple pattern, unlike kLa in the rotational shaking vessel, which increases linearly with increasing frequency. The kLa of the reciprocally shaking vessel was larger than the kLa of the rotational shaking vessel, and as the kLa value increased, the difference between them increased sharply. As a result, the oxygen transfer rate in the reciprocal motion was greater than that of the rotational motion, and could be correlated with the required power per unit volume.

• Korean Chemical Engineering Research
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• v.53 no.2
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• pp.174-180
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• 2015

The flow pattern and the solid-liquid mass transfer coefficient in figure-eight shaking vessels were observed by experimental method. The flow patterns, mixing time, power consumption and mass transfer coefficient in the figureeight shaking vessels changed irregularly with increase in the shaking frequency. Any frequency, even in the Fr = 0.095 or more became clear experimentally. The region of the optimum operating condition of the figure-eight shaking was larger than that of the reciprocal shaking. The solid-liquid mass transfer coefficient was correlated with the same correlation as that of the rotary shaking vessel of existing. The gas-liquid mass transfer coefficient of the figure-eight shaking vessel was also correlated with the same type of correlation as that of the rotary shaking vessel of existing.

• Korean Journal of Pharmacognosy
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• v.16 no.3
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• pp.171-174
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• 1985

Panax ginseng root has been widely used as an important drug for thousands years in China, Korea and Japan. The main effective components of ginseng have been believed to be saponins. However, ginseng cultivation is very difficult and needs many years for growth. It has already been shown that Panax ginseng callus produces a considerable amount of the same kinds of saponins as in intact plants. Various culture conditions were examined for increased production of ginseng saponins by cell culture. The saponin contents and the growth rates in two cell lines of ginseng callus were compared in static and suspension cultures, rotary and reciprocal shaking cultures. It was shown that the growth rate in rotary shaking cultures of D5-B2K-B2K callus was the highest and ginseng saponin production was most effective in reciprocal cultures of D5-B2K-B2K callus. The saponin content per fresh weight of the culture was 1.03 times higher than that of the fresh ginseng root.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.131-135
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• 2002

Acetobacter xylinum KJ1 efficiently producing bacterial cellulose(BC) in shaking culture was isolated from a rotten grape. The strain was used to investigate optimum operating conditions for increasing BC production and factorial design model was employed for the optimization. The results of experiments were statistically analyzed by SAS program. Reciprocal effects of each factors(carbon source concentration, shaking speeds(rpm), oxygen pressure, and CSL concentration) and culture condition of BC production were examined by getting regression equation of the dependent variable. Comparisons between experimental results and predicted results about BC concentration were done in total 24 experiments by combination of each factors using SAS program, and the correlation coefficients of BC concentration and BC yield were 0.91 and 0.81, respectively. The agitated cultures were performed in various operation conditions of factors which affected considerably to BC production in jar fermentor. The results showed that BC concentration was 11.67g/ L in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 460rpm : 0.28 : 6%. On the other hand BC yield was 0.42g/g in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 564rpm : 0.21 : 2%. The BC production could be enhanced up to more than 65.3% by factorial design. The result of a verifying experiment under the optimal conditions determined by the factorial design to the BC production showed that the model was appropriate by obtaining BC concentration of 11.02g/L in the optimum condition

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.6
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• pp.1020-1026
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• 1994

Pseudomonas plycolor was used to investigated the optimal culture condition to examine the various properties of superoxide dismutase (SOD). this SOD was inhibited by $H_2O_2$, azide ion, but not by cyanide ion. This result indicates that the enzyme might be a Fe-SOD. The composition of optimal culture medium for the enzyme production was 3% of glycerin, 1% of polypeptone, 0.5% of meat extract, 0.2% of KCI and the initial ph was 9.0 . The cultivation for the enzyme production was carried out in 500ml shaking flask containing 100ml of the optimal medium at $30^{\circ}C$ on a reciprocal shaker. The enzyme production reached maximum at 15hrs of cultivation and then declined sharply afterward.

• Journal of Life Science
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• v.8 no.1
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• pp.1-7
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• 1998

The mold YH-69 producing pigments was isolated from soil, and identified as Monascus sp.. For the production of the pigments from the mold, the best condition was observed with 6% rice powder with 200 mesh, 0.2% yeast extract, 0.3% ammonium sulfate, and 0.02% ZnSO4 at pH 5 and 30$\circ$ for 6 day with reciprocal shaking.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.10 no.S_1
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• pp.41-45
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• 2001

Pseudomonas aeruginosa EMS1, isolated from activated sludge, was able to grow an produce a biosurfactant on 4.5 % soybean oil, used as the source of energy and carbon. Pseudomonas aeruginosa EMS1 was cultivated at 3$0^{\circ}C$ in a reciprocal shaking incubator, and the highest biosurfactant production was observed after 3 days. Furthermore, Pseudomonas aeruginosa EMS1 was also able to use whey as a co-substrate for biosurfactant production and growth

• KSBB Journal
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• v.17 no.3
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• pp.228-234
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• 2002

Acetobacter xylinum KJ1 efficiently producing bacterial cellulose(BC) In shaking culture was isolated from a rotten grape. The strain was used to investigate optimum operating conditions for increasing BC production and factorial design model was employed for the optimization. The results of experiments were statistically analyzed by SAS program. Reciprocal effects of each factors(carbon source concentration, shaking speeds(rpm), oxygen pressure, and CSL concentration) and culture condition of BC production were examined by getting regression equation of the dependent variable. Comparisons between experimental results and predicted results about BC concentration were done in total 24 experiments by combination of each factors using SAS program, and the correlation coefficients of BC concentration and BC yield were 0.91 and 0.81, respectively. The agitated cultures were peformed in various operation conditions of factors which affected considerably to BC production in jar fermentor. The results showed that BC concentration was 11.67 g/L in 80 hours cultivation under the condition of carbon source concentration : shaking speeds(rpm) : oxygen pressure : CSL concentration : 4% : 460 rpm : 0.28 : 6%. On the other hand BC yield was 0.42 g/g in 80 hours cultivation under the condition of carbon source concentration : shaking speeds(rpm) : oxygen pressure : CSL concentration : 4% : 564 rpm : 0.21 : 2%. The BC production could be enhanced up to more than 2.4 times by factorial design. The result of a verifying experiment under the optimal conditions determined by the factorial design to the BC production showed that the model was appropriate by obtaining BC concentration of 11.47 g/L in the optimum condition.

• Korean Journal of Microbiology
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• v.34 no.1_2
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• pp.37-42
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• 1998

The microorganisms degrading levan were screened from soil. The isolated strain produced levanase releasing single oligosacchride from levan. The optimum cultural medium for levanase production (g/l) was composed of 0.5% levan, 0.1% $K_2HPO_4$, 0.05% NaCl, 0.3% $NaNO_3$, 0.3% yeast extract (pH 8.0). The cultivation for levanase production was carried out in 500 ml shaking flask containing 50 ml of the optimum medium at $30^{\circ}C$ on a reciprocal shaker, and the highest levanase production was observed after 54 hours of cultivation. The levanase hydrolyzed levan into single oligosaccharide. The product purified by chilled EtOH precipitation and gel filtration was detected as a single peak on HPLC analysis. The oligosaccharides formed by enzyme reaction was identified as levanheptaose (DP7) by HPLC and by ESI-MASS.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.1
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• pp.27-35
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• 2010

This paper presents the vibration control performance of the smart passive control system to suppress the undesired vibration of the structure subjected to the earthquake loadings. Smart passive control system is the MR damper-based control system augmented with electromagnetic induction(EMI) device which consists of permanent magnets and solenoid coils. According to the Faraday's law of electromagnetic induction, an EMI device produces electrical energy from the mechanical energy due to the reciprocal motions of the structure and provide it to the MR damper. The smart passive control system can be the simple and easy to implement and maintain control system by replacing the feedback control system including sensors, controllers and external power sources of the conventional MR damper-based semiactive control system with the EMI device. The control performance of the smart passive control system is evaluated through the set of shaking table test considering the various historical earthquake loadings.