• Korean Journal of Veterinary Service
• /
• v.34 no.4
• /
• pp.321-331
• /
• 2011

Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical-based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2004.10a
• /
• pp.993-997
• /
• 2004

A data error inspection algorithm for wireless digital data communication was developed. Original data converted By wireless digital data error inspection algorithm. Wireless digital data is high possibility to get distortion and lose by noise and barrier on wireless. If the data check damaged and lost at receiver, can't make it clear and can't judge whether this data is right or not. Therefore, by wireless transmission data need the data error inspection algorithm in order to decrease the data distortion and lose and to monitoring the transmission data as real time. This study consists of RF station for wireless transmission, Water Level Meter station for water level measurement and Error inspection algorithm for error check of transmission data. This study is also that investigation and search for error inspection algorithm in order to wireless digital data transmission in condition of the least data's damage and lose. Designed transmitter and receiver with one - chip micro process to protect to swell the volume of circuit. Had designed RF transmitter - receiver station simply by means of ATMEL one - chip micro processing the systems. Used 10mW of the best RF power and 448MHz-449MHz on frequency band which is open to public touse free within the limited power.

• Journal of Korea Multimedia Society
• /
• v.14 no.12
• /
• pp.1572-1582
• /
• 2011

The rapid growth of UnderWater Acoustic Sensor Networks (UW-ASNs) has led researchers to enhance underwater MAC protocols against limitations existing in underwater environment. We propose the customized robust real-time packet inspection mechanism with addressing the problem of the search for the data packet loss and network performance quality analysis in UW-ASNs, and describe our experiences using this approach. The goal of this work is to provide a framework to assess the network real-time performance quality. We propose a customized and adaptive mechanism to detect, monitor and analyze the data packets according to the MAC protocol standards in UW-ASNs. The packet analyzing method and software we propose is easy to implement, maintain, update and enhance. We take input stream as real data packets from sniffer node in capture mode and perform fully analysis. We were interested in developing software and hardware designed tool with the same capabilities which almost all terrestrial network packet sniffers have. Experimental results confirm that the best way to achieve maximum performance requires the most adaptive algorithm. In this paper, we present and offer the proposed packet analyzer, which can be effectively used for implementing underwater MAC protocols.

• The Journal of the Korea Contents Association
• /
• v.18 no.3
• /
• pp.101-113
• /
• 2018

Systems such as military inspection equipment which it is important to acquire and evaluate data in real-time should be able to real-time processing at the operating system level. As technology advances, there is a demand for replacing existing equipment with mobile device, but mobile devices with Android are not suitable for systems requiring real-time performance. On Android, garbage collection ensures free memory, while other tasks are interrupted while this task is performed, which cannot guarantee periodicity of particular tasks. In this paper, we designed and implemented a structure to control execution garbage collection of Android to solve this problem. Real-time performance is ensured by controlling garbage collection during the time required for real-time operation, and RTiK(Real-Time implanted Kernel) is applied to ensure real-time performance on Android. In order to evaluate the performance, we measured the call period of the 5ms period task, and, only 34.31% of the task was guaranteed before the control, but the task period of 98.18% was satisfied through control, providing real-time performance to Android.

• IEMEK Journal of Embedded Systems and Applications
• /
• v.18 no.3
• /
• pp.117-124
• /
• 2023

In the machine vision inspection equipment, diameter measurement is important process in inspection of cylindrical object. However, machine vision inspection equipment requires complex algorithm processing such as camera distortion correction and perspective distortion correction, and the increase in processing time and cost required for precise diameter measurement. In this paper, we proposed the algorithm for diameter measurement of cylindrical object using the laser displacement sensor. In order to fit circle for given four input outer points, grid search algorithms using root-mean-square error and mean-absolute error are applied and compared. To solve the limitations of the grid search algorithm, we finally apply the singular-value-decomposition based circle fitting algorithm. In order to compare the performance of the algorithms, we generated the pseudo data of the outer points of the cylindrical object and applied each algorithm. As a result of the experiment, the grid search using root-mean-square error confirmed stable measurement results, but it was confirmed that real-time processing was difficult as the execution time was 10.8059 second. The execution time of mean-absolute error algorithm was greatly improved as 0.3639 second, but there was no weight according to the distance, so the result of algorithm is abnormal. On the other hand, the singular-value-decomposition method was not affected by the grid and could not only obtain precise detection results, but also confirmed a very good execution time of 0.6 millisecond.

• Computers and Concrete
• /
• v.23 no.6
• /
• pp.445-457
• /
• 2019

Cracks are an important distress of concrete bridges, and may reduce the life and safety of bridges. However, the traditional manual crack detection means highly depend on the experience of inspectors. Furthermore, it is time-consuming, expensive, and often unsafe when inaccessible position of bridge is to be assessed, such as viaduct pier. To solve this question, the real-time automatic crack detecting system with unmanned aerial vehicle (UAV) become a choice. This paper designs a new automatic detection system based on real-time comprehensive image processing for bridge crack. It has small size, light weight, low power consumption and can be carried on a small UAV for real-time data acquisition and processing. The real-time comprehensive image processing algorithm used in this detection system combines the advantage of connected domain area, shape extremum, morphology and support vector data description (SVDD). The performance and validity of the proposed algorithm and system are verified. Compared with other detection method, the proposed system can effectively detect cracks with high detection accuracy and high speed. The designed system in this paper is suitable for practical engineering applications.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.32 no.6
• /
• pp.696-702
• /
• 2012

In this study, a noncontact laser ultrasonic system is proposed for rail defect detection. An Nd-Yag pulse laser is used for generation of ultrasonic waves, and the corresponding ultrasonic responses are measured by a laser Doppler vibrometer. For the detection of rail surface damages, the shape of the excitation laser beam is transformed into a line. On the other hand, a point source laser beam is used for the inspection of defects inside a rail head. Then, the interactions of propagating ultrasonic waves with defects are examined using actual rail specimens. Amplitude attenuation was mainly observed for a surface crack, and reflections were most noticeable from an internal damage. Finally, opportunities and challenges associated with real-time rail inspection from a high-speed train are discussed.

• Journal of the Korean Society of Safety
• /
• v.18 no.1
• /
• pp.56-63
• /
• 2003

During the last decade, effort has been made f3r reducing maintenance cost for aged equipments and ensuring safety, efficiency and profitability of petrochemical and refinery plants. Hence, it was required to develop advanced methods which meet this need. RBI(Risk Based Inspection) methodology is one of the most promising technology satisfying the requirements in the field of integrity management. In this study, a quantitative assessment algorithm fir RBI based on the API 581 code was reconstructed for developing an RBI software. The user-friendly realRBI software is developed with a module for evaluating quantitative risk md financial risk using the potential consequence and the likelihood. Also, inspection planning module for inspection time and inspection method are included in it.

• Journal of Mechanical Science and Technology
• /
• v.14 no.10
• /
• pp.1072-1080
• /
• 2000

Leakage magnetic flux (LMF) is widely used for non-contact detection of cracks. The combination of optics and LMF offers advantages such as real time inspection, elimination of electrical noise, high spatial resolution, etc. This paper describes a new nondestructive evaluation method based on an original magneto-optical inspection system, which uses a magneto-optical sensor, LMF, and an improved magnetization method. The improved magnetization method has the following characteristics: high observation sensitivity, independence of the crack orientation, and precise transcription of the geometry of a complex crack. The use of vertical magnetization enables the visualization of the length and width of a crack. The inspection system provides the images of the crack, and shows a possibility for the computation of its depth.

• Journal of Institute of Control, Robotics and Systems
• /
• v.2 no.3
• /
• pp.236-241
• /
• 1996

This paper presents a new eddy current testing system for inspecting tubes of steam generator in nuclear power plant. The proposed system adopted embedded expert system concept to automate tasks of the inspection such as inspection planning and flaw signal interpretation, and integrated all the tasks into a client/server type computing architecture using database management system. Therefore, human factor errors occurred during inspection could be minimized and the inspection data could be transferred in real-time. As a result, we can increase the level of inspection confidence and the productivity of a personal inspector. A prototype of the proposed system has been developed for 5 years and the test operation has been performed in domestic nuclear power plants.