• Korean Journal of Microbiology
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• v.43 no.1
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• pp.23-30
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• 2007

The most rapid Real-time PCR based detection method for Avian influenza A virus (AIV) subtype H5N1 was developed. The target DNA sequence in this study was deduced from H5N1 subtype-specific 387 bp partial gene of hemagglutinin, and was synthesized by using PCR-based gene synthesis on the ground of safety. Real-Time PCR was performed by $GenSpector^{TM}$ using microchip-based, total $1{\mu}l$ of reaction mixture with extremely short time in each steps in PCR. The detection including PCR-amplication and analysis of melting temperature was totally completed within 13 min. The H5N1-specific 189 bp PCR product was correctly amplified until 2.4 molecules of hemagglutinin gene as minimum of templates. This kind of PCR was designated as Quick Real-Time PCR in this study and it could be applied to detect not only AIV H5N1, but also other pathogens using PCR-based detection.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.5 no.3
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• pp.291-299
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• 2015

With recent surge of attention to heating and air conditioning energy consumption, the need for evaluating performance of heating and air conditioning energy is also on the rise. This research aims to propose daily-life based evaluation method as an alternative to existing one, as well as to apply the method in real life to prove its validity. The results are as following. 1) I studied temperatures preferred by persons indoors and appropriate level of temperature, and the results show that properties of such persons such as age is directly linked with pleasantness of the room, but the issue lies with the fact that such properties are not considered in existing performance evaluation of heating and air conditioning. 2) Daily life based evaluation proposed herein reflects such properties of persons indoors. It controls heating / air conditioning devices installed in the test bed with the same size as a real life room to get quantitative and visual performances in our daily life as well as simple temperature information. 3) To verify validity of daily life based evaluation, I conducted different evaluation sessions with and without a blind and also based on ages of persons indoors. Results based on properties of persons showed difference of 77.6%, leading to effective analysis of energy consumption pattern by heating / air conditioning devices. This research takes significance in that it comes with a new performance evaluation method based on real life, and I gather that further studies are required to develop more multilateral performance evaluation in order to verify and improve technology for reduction of energy consumption.

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.24.1-24.1
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• 2006

• Journal of the Korean Wood Science and Technology
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• v.39 no.5
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• pp.437-443
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• 2011

In Korea, there has been a traditional post and beam wood construction with large roof load. Because a large friction is generated in wooden joint or members, it is important to evaluate the friction between wood members according to wood direction. Because most of studies have been concerned with friction between wood and steel, excluding effect of real area of contact, there are a few studies on the friction between wood members. The object of this study was to evaluate friction or coefficient of friction according to normal force and real area of contact of wood. With Japanese larch (Larix kaempferi) test specimens, five steps of normal force and combinations of test were prepared. Results indicated that normal force had almost no affection on the friction, however there was difference about friction or coefficient of friction according to real contact conditions of wood grain and contact area.

• Journal of the Korea Society of Computer and Information
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• v.21 no.1
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• pp.85-90
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• 2016

In this paper, the biometric data is transmitted in real time from the IoT environment is runoff, forgery, alteration, prevention of the factors that can be generated from a denial-of-service in advance, and the security strategy for the biometric data to protect the biometric data secure from security threats offer. The convenience of living in our surroundings to life with the development of ubiquitous computing and smart devices are available in real-time. And is also increasing interest in the IOT. IOT environment is giving the convenience of life. However, security threats to privacy also are exposed for 24 hours. This paper examines the security threats to biological data to be transmitted in real time from IOT environment. The technology for such security requirements and security technology according to the analysis of the threat. And with respect to the biometric data transmitted in real time on the IoT environment proposes a security strategy to ensure the stability against security threats and described with respect to its efficiency.

• Corrosion Science and Technology
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• v.8 no.6
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• pp.243-250
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• 2009

When a steel sheet pipe applied to marine environment, an anti-corrosive coating should be treated to obtain long-term life-time for steels, especially, splash zone. Although anti-corrosive property of coatings is required to be tested in real marine environment, it is difficult because of long test time such as 20 years or more time. Therefore, we used cyclic corrosion tester in a laboratory, which has similar conditions with salt-dry-wet process such as real marine environment. Anti-corrosive properties of the coatings and two steels were tested their anti-corrosive properties under cyclic corrosion test conditions(KS D ISO 14993) and the results were compared with estimate life-time in real marine environment. According to cyclic corrosion test, accelerated corrosive factor of each anti-corrosive coating was investigated accelerated corrosive factor from impedance with EIS method. Accelerated corrosive factor of type SS400 carbon steel and A690 was also investigated their accelerated corrosive factor from the regression curves of weigh loss results. One of the anti-corrosive coatings showed about 50 years life-time compared with standard sample life-time. Carbon steel SS400 showed from 0.1 mm/yr to 0.06 mm/yr as its corrosion rate.

• Journal of the Korean School Mathematics Society
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• v.10 no.1
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• pp.45-69
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• 2007

In the 21st century, knowledge-based and information-based society requires not just the capability of applying mathematics simply but mathematical power such as problem-solving ability which composes and solves problems using mathematical knowledge in real-life and fields of various subjects. However, to develop mathematical power, we need various teaching and learning methods which raise basic mathematical knowledge, the inference capability, problem- solving ability, the flexibility of thinking, the expressing and transforming ability of mathematical ideas, perseverance, interest, intellectual curiosity, and creativity. In this paper, we develop the teaching-learning plans based on real life using the various methods of learning and then we analyze the change of students's cognition of mathematics and the students's reaction of the class.

• Journal of the Korea Society of Computer and Information
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• v.25 no.11
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• pp.255-262
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• 2020

The purpose of this study is to find out an effective way for students with developmental disabilities to use smartphone applications as a social real life tools in a society. For the purpose of this study, the researcher reviewed relevant previous literature on topics such as "students with developmental disabilities, information society, use of smartphone, and application use". The results of this study are as follows. First, the researcher describes strategies to support students with developmental disabilities living in an information society to improve their social integration ability by using smartphone. Second, it describes the types of smartphone applications that could support the social real life of students with developmental disabilities. Third, it describes the needs of smartphone applications to improve social adaptability of students with developmental disabilities. The results of this study will lead students with developmental disabilities to learn and use various functions of smartphone in the information society to interact with people around them and improve their communication skills. In addition, the results can be used as basic data to develop and support appropriate applications that can help vocational rehabilitation and social integration of students with developmental disabilities.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.17 no.2
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• pp.33-37
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• 2011

Real time control logic of microbial shelf life of a perishable food, seasoned pork meat has been formulated which exploits monitoring of $CO_2$ concentration of the package. The potential of the proposed logic was examined for storage at dynamic temperature conditions. The start of increase in $CO_2$ production rate from the food or rate of package $CO_2$ concentration change was found to coincide with the point of microbial quality limit and could be used as an index of microbial shelf life determination. This also corresponded to lag time of $CO_2$ concentration change or time for the $CO_2$ concentration to reach a certain value. The application potential of the proposed logic was confirmed for a sensor system to measure on real time and transmit the $CO_2$ concentration wireless to the computer system.

• Journal of Microbiology and Biotechnology
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• v.31 no.2
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• pp.280-289
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• 2021

Genetic markers currently used for the discrimination of Lactobacillus delbrueckii subspecies have low efficiency for identification at subspecies level. Therefore, our objective in this study was to select novel genetic markers for accurate identification and discrimination of six L. delbrueckii subspecies based on pangenome analysis. We evaluated L. delbrueckii genomes to avoid making incorrect conclusions in the process of selecting genetic markers due to mislabeled genomes. Genome analysis showed that two genomes of L. delbrueckii subspecies deposited at NCBI were misidentified. Based on these results, subspecies-specific genetic markers were selected by comparing the core and pangenomes. Genetic markers were confirmed to be specific for 59,196,562 genome sequences via in silico analysis. They were found in all strains of the same subspecies, but not in other subspecies or bacterial strains. These genetic markers also could be used to accurately identify genomes at the subspecies level for genomes known at the species level. A real-time PCR method for detecting three main subspecies (L. delbrueckii subsp. delbrueckii, lactis, and bulgaricus) was developed to cost-effectively identify them using genetic markers. Results showed 100% specificity for each subspecies. These genetic markers could differentiate each subspecies from 44 other lactic acid bacteria. This real-time PCR method was then applied to monitor 26 probiotics and dairy products. It was also used to identify 64 unknown strains isolated from raw milk samples and dairy products. Results confirmed that unknown isolates and subspecies contained in the product could be accurately identified using this real-time PCR method.