• International Journal of Stem Cells
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• v.15 no.3
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• pp.324-333
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• 2022

Background and Objectives: This study was to investigate the role of microRNA-29a-3p (miR-29a-3p) in human bone marrow mesenchymal stem cells (hBMSCs), and its relationship with steroid-associated osteonecrosis. Methods and Results: The online tool GEO2R was used to screen out the differentially expressed genes (DEGs) in GSE123568 dataset. Quantitative real time-polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-29a-3p, forkhead box O3 (FOXO3), alkaline phosphatase (ALP), bone gamma-carboxyglutamate protein (OCN) and RUNX family transcription factor 2 (Runx2) in the hBMSCs isolated from the patients with steroid-associated osteonecrosis. CCK-8 assay was executed to measure cell viability; western blot assay was utilized to detect FOXO3, ALP, Runx2, OCN and β-catenin expression. Cell apoptosis and cell cycle were detected by flow cytometry. Immunofluorescence assay was used to detect the sub-cellular localization of β-catenin. Bioinformatics analysis and luciferase reporter gene assay were performed to confirm whether miR-29a-3p can combine with FOXO3 3'UTR. MiR-29a-3p was markedly up-regulated in the hBMSCs of patients with steroid-associated osteonecrosis, while FOXO3 mRNA was significantly down-regulated. Transfection of miR-29a-3p mimics significantly inhibited the hBMSCs' proliferation, osteogenic differentiation markers' expressions, including ALP, Runx2, OCN, and repressed the ALP activity, as well as promoted cell apoptosis and cell-cycle arrest. FOXO3 was identified as a target gene of miR-29a-3p, and miR-29a-3p can inhibit the expression of FOXO3 and β-catenin, and inhibition of miR-29a-3p promoted translocation of β-catenin to the nucleus. Conclusions: MiR-29a-3p can modulate FOXO3 expression and Wnt/β-catenin signaling to inhibit viability and osteogenic differentiation of hBMSCs, thereby promoting the development of steroid-associated osteonecrosis.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.23 no.4
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• pp.37-53
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• 2024

This study selected bicycle road hazard detection factors for mobile IoT sensor device operation and developed service application plans. Twelve bicycle road hazard detection factors were derived through a focused group interview, and a fuzzy AHP-based importance analysis was conducted on 30 road and transportation experts. As a result, 'damage to pavement' (1st overall) and 'environmental obstacle' (2nd) with low visibility but a high risk of accidents were selected the most. The factors in terms of facility management, such as 'disconnected route occurrence' (4th), 'artificial obstacle' (5th), 'effective width' (6th), and 'poor drainage' (7th), were selected as the upper and middle areas. Factors that are not direct accident-inducing factors, such as 'loss of road markings' (11th) and 'free space width' (12th), were selected the least. Based on this, a plan was presented to apply the bicycle road hazard detection service and a service operation strategy according to real-time performance. Nevertheless, follow-up studies, such as human behavioral analysis based on bicycle operators, analysis according to the bicycle road type, service demonstration, and pilot operation, will be needed to develop safe bicycle road management is expected.

• 한국학연구
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• no.55
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• pp.455-489
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• 2019

In the wake of the disaster, Joseon implemented soliciting suggestions(求言) to listen to opinions from bureaucrats and the nobility. Through the reign of King Seongjong, the real function of soliciting suggestions as a public opinion has been strengthened. The establishment of the power structure centered on the cheong'yo-jik posts(淸要職) has stimulated a public opinion at all times and increased the function of the eungji-sangso(應旨上疏) as a public opinion. Soliciting suggestions were used as another press opportunity for Cheong'yo-jik posts. It also raised sensitive issues that are usually difficult to mention through eungji-sangso. At the same time, the principle of "not punishing the content of the eungji-sangso by taking issue with it" was also continuously emphasized. As sensitive issues were expressed through the eungji-sangso, the political situation created a stir. Meanwhile, it was burdensome for the monarch to expand the function of the eungji-sangso. In response, King Yeonsan and King Jungjong responded passively to the operation of the eungji-sangso system and punished those who raised the eungji-sangso. Still, the operation of the old system has become a meaningful opportunity to provide another press opportunity. Still, the operation of the soliciting suggestions system has meaning as another mechanism for press activities. In the long run, it has become an opportunity for the nobility without an official position to express their opinions on pending state affairs. And this helped expand the media presence.

• Journal of Wind Energy
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• v.13 no.4
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• pp.26-41
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• 2022

Three similarities (i.e., geometrical similarity, kinematic similarity and dynamic similarity) between a prototype and model must be satisfied to perform an experiment for a floating offshore wind turbine (FOWT). For dynamic similarity, most of the model tests in ocean engineering basins are performed based on the Froude number, so the scale effect for the wind turbine of an FOWT occurs by different Reynolds numbers between the prototype and model. In this study, various model test techniques for overcoming the scale effect of the wind turbine part of the FOWT are investigated. Firstly, model test techniques using simple approaches are reviewed, and the advantages and disadvantages of the simple approaches are summarized. Secondly, the model test techniques in recent projects that apply improved approaches are introduced including advantages and disadvantages. Finally, new approaches applying digitalization are reviewed, and the characteristics of the new approaches are introduced.

• International Journal of Stem Cells
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• v.17 no.3
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• pp.309-318
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• 2024

Nucleotide-binding oligomerization domain 1 (NOD1), a cytosolic pattern recognition receptor protein, plays a crucial role in innate immune responses. However, the functional expression of NOD1 in mesenchymal stem cells (MSCs) derived from endometriosis remains unclear. The aim of this study was to explore the functions of NOD1 in ectopic endometrial lesions. Tissues and MSCs were isolated from both normal endometrium and endometriosis. Immunohistochemistry and real time quantitative polymerase chain reaction (RT-qPCR) were used to determine the expression of NOD1 in the tissues/MSCs. Quantification of various cytokines was performed using RT-qPCR and enzyme-linked immunosorbent assay. To confirm the proliferation, invasion/migration, and apoptotic viabilities of the samples, Cell Counting Kit-8, clonogenic formation, transwell assays, and apoptotic experiments were conducted. Higher levels of NOD1 expression were detected in the ectopic-MSCs obtained from endometriosis compared to those from the endometrium. The expression of interleukin-8 was higher in the ectopic-MSCs than in the eutopic-MSCs. Pretreatment with NOD1 agonist significantly enhanced the proliferation and invasion/migration of eutopic-MSCs. Additionally, the NOD1 inhibitor ML-130 significantly reduced the proliferation, clone formation, invasion, and migration abilities of the ectopic-MSCs, having no effect on their apoptosis capacity. Our findings suggest that the expression of NOD1 in ectopic-MSCs may contribute to the progression of ectopic endometrial lesions.

• Journal of the Korea Society of Computer and Information
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• v.29 no.9
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• pp.179-186
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• 2024

In this paper, we design and implement an Internet of Things (IoT)-based chick incubation system. The system consists of three key components: the IoT incubator, the IoT server system, and the smartphone application. The IoT incubator is composed of an Arduino board, temperature and humidity sensors, a temperature and humidity controller, a ventilation controller, and an egg turning controller. The temperature and humidity sensors measure the temperature and humidity inside the IoT incubator and send the data to the temperature and humidity controller on the Arduino board. Additionally, it provides the function of transmitting temperature, humidity, and control history data to the IoT server via WiFi. It also offers automatic control of ventilation, egg turning, and temperature and humidity on a daily basis. The IoT server system receives data from the incubator, stores it in a database, and provides query data upon request from the smartphone. The smartphone application retrieves historical data through the server and monitors the temperature and humidity data of the IoT incubator in real-time, controlling the IoT incubator to ensure that the set temperature and humidity ranges are maintained. If the temperature and humidity data deviate from the set ranges, it sends alarms and emergency messages to the user. The IoT-based chick incubation system developed in this paper is a low-cost model due to its reduced manufacturing cost, making it highly beneficial for self-sustaining poultry farms.

• Journal of IKEEE
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• v.28 no.3
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• pp.458-464
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• 2024

This study proposes a fault-tolerant caching system to address the issue of caching content imbalance caused by the dynamic departure and participation of cache servers in a heterogeneous cache server network, and validates it in both real and virtual environments. With the increase of large-scale media content requiring various types and resolutions, the necessity of cache servers as key components to reduce response time to user requests and alleviate network load has been growing. In particular, research on heterogeneous cache server networks utilizing edge computing and low-power devices has been actively conducted recently. However, in such environments, the irregular departure and participation of cache servers can occur frequently, leading to content imbalance among the cache servers deployed in the network, which can degrade the performance of the cache server network. The fault-tolerant caching algorithm proposed in this study ensures stable service quality by maintaining balance among media contents even when cache servers depart. Experimental results confirmed that the proposed algorithm effectively maintains content distribution despite the departure of cache servers. Additionally, we built a network composed of seven heterogeneous cache servers to verify the practicality of the proposed caching system and demonstrated its performance and scalability through a large-scale cache server network in a virtual environment.

• The Transactions of the Korea Information Processing Society
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• v.13 no.9
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• pp.444-452
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• 2024

This study proposes a deep learning architecture optimized for fire detection derived through Layer Importance Evaluation. In order to solve the problem of unnecessary complexity and operation of the existing Convolutional Neural Network (CNN)-based fire detection system, the operation of the inner layer of the model based on the weight and activation values was analyzed through the Layer Importance Evaluation technique, the layer with a high contribution to fire detection was identified, and the model was reconstructed only with the identified layer, and the performance indicators were compared and analyzed with the existing model. After learning the fire data using four transfer learning models: Xception, VGG19, ResNet, and EfficientNetB5, the Layer Importance Evaluation technique was applied to analyze the weight and activation value of each layer, and then a new model was constructed by selecting the top rank layers with the highest contribution. As a result of the study, it was confirmed that the implemented architecture maintains the same performance with parameters that are about 80% lighter than the existing model, and can contribute to increasing the efficiency of fire monitoring equipment by outputting the same performance in accuracy, loss, and confusion matrix indicators compared to conventional complex transfer learning models while having a learning speed of about 3 to 5 times faster.

• Research in Plant Disease
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• v.30 no.3
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• pp.294-299
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• 2024

Polymerase chain reaction (PCR) methods, including conventional PCR (cPCR) and quantitative real-time PCR (qRT-PCR), with both plasmid- and chromosome-targeting primers, are currently the most reliable methods for detecting Erwinia amylovora due to their high sensitivity and specificity. Despite qRT-PCR's quantitative advantage, cPCR remains an attractive method to detect this bacterium in initial screenings of suspected host plants, as it is cost-effective and does not require skilled personnel in well-equipped laboratories. This study aimed to significantly improve cPCR robustness via application of bovine serum albumin (BSA) as a PCR facilitator, with a modified EaF/R primer pair, as previously reported. Experiments have shown that simple supplementation with BSA (10 mg/ml) enhances cPCR reactions using templates such as genomic DNA, bacterial cells, and infected symptomless host organs, including immature apple fruits and seedlings, with EaF/R primers. The cPCR method described in this study is simple, specific, and reliable, and can be applied in routine assays to diagnose fire blight.

• Korean Journal of Optics and Photonics
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• v.35 no.6
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• pp.276-282
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• 2024

Optogenetics is a technology that uses light to precisely control and analyze neural activity with high spatial and temporal resolution, making it useful for real-time investigation of specific neural circuits and their functions. In this paper, we introduce optogenetic applications using NeuroWeb, a neural probe composed of graphene and hexagonal boron nitride. NeuroWeb's thinness of 100 nm and open lattice structure allow for high flexibility, strong adhesion, and superior transparency. Using these capabilities of NeuroWeb, we perform optogenetic research by controlling neural activity in specific regions via optical stimulation. In particular, the neural interactions between the somatosensory cortex and the cerebellum are generated by optical stimulation, and signal transmission times between these regions are precisely measured. The transmission times from the somatosensory cortex to the cerebellum and from the cerebellum to the somatosensory cortex are 6.33 ms and 5.88 ms, respectively. Therefore, by combining the NeuroWeb neural probe with optogenetics experimental techniques, we expect to be able to understand complex neural circuits and conduct future research on neurological diseases such as Alzheimer's and Parkinson's.