• 제목/요약/키워드: Reading activation

검색결과 72건 처리시간 0.022초

The Yin and Yang of RNA surveillance in B lymphocytes and antibody-secreting plasma cells

  • Lambert, Jean-Marie;Srour, Nivine;Delpy, Laurent
    • BMB Reports
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    • 제52권12호
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    • pp.671-678
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    • 2019
  • The random V(D)J recombination process ensures the diversity of the primary immunoglobulin (Ig) repertoire. In two thirds of cases, imprecise recombination between variable (V), diversity (D), and joining (J) segments induces a frameshift in the open reading frame that leads to the appearance of premature termination codons (PTCs). Thus, many B lineage cells harbour biallelic V(D)J-rearrangements of Ig heavy or light chain genes, with a productively-recombined allele encoding the functional Ig chain and a nonproductive allele potentially encoding truncated Ig polypeptides. Since the pattern of Ig gene expression is mostly biallelic, transcription initiated from nonproductive Ig alleles generates considerable amounts of primary transcripts with out-of-frame V(D)J junctions. How RNA surveillance pathways cooperate to control the noise from nonproductive Ig genes will be discussed in this review, focusing on the benefits of nonsense- mediated mRNA decay (NMD) activation during B-cell development and detrimental effects of nonsense-associated altered splicing (NAS) in terminally differentiated plasma cells.

간호학생의 효과적인 자율학습을 위한 조사연구 (A Study on the Effective Independent Study of Nursing Student)

  • 김광주;이향련
    • 대한간호학회지
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    • 제8권1호
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    • pp.16-42
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    • 1978
  • This survey was made for a month starting from November 15 to December 15, 1977 covering 711 students taking the junior. (3rd-yea.) and the senior. (4th-year) at nine college of nursing in Seoul concerning their perception and Attitude toward the profession of nursing, motive for the necessity of learning, environment of study, attitude of study and particulars relevant with study performance, particulars of library, references and reading, assignments and particulars of the degree of confidence for the learning achievement. Through the survey of the above Particulars, the following results were obtained by classifying all subject matters and by analysing motive of the selection of their course, awarding or not awarding of scholarships. 1. General characteristics: it was revealed that 406 students (57.1%) were attending at the junior. while 305 students (42.9%) were taking the senior. Thus, the total number was 711 and their average age was 21.4 years. Their dwelling category was; 73.9 percent of them resided at their parent's home, 214 students (30.1%) were awarded with scholarships. The reason to be attracted by nursing science was the possibility of continuing social life after graduation (43.5%). 2. Their perception and attitude toward the profession of nursing: According to the perception of profession by the students of each grade, students of the 4th grade showed comparatively strong conception. Also, students of the 4th grade showed more positive attitude in the purchase and reading of magazines relative with the science of nursing, in the reading of Code for Nurses and in their interest in the activity of nursing field. For the necessity of mission of nurse, 97.7 percent of the entire number of students covered responded to necessity. For the necessity of the particular humanity and particularity in the character of nurses, 95.8 percent of those students responded to necessity. By the each grade, students of the 4th grade showed more response. 3. As to professional field desired after completing the professional course: 57 percent of those students desired for clinician nurse while 55 percents desired for community health-nurse. 4. As to the environment of study: they were mostly satisfied with their present residential environment. However, they complained of inconvenience at their lecture-halls. Students of the 3rd grade showed more complain. As to their attitude toward the adjustment of environment of study, they showed a affirmative response. As to the opinion of factors which interfere with their study, comparatively strong response was showed in their scepticism in the science of nursing, insufficient comprehension in general learning, relation with professors n4 discrepancy in the method of study. According to opinions of students at each grade, students of the 4th grade showed more scepticism. 5. Particulars relative with their attitude and performance of study : As to their knowledge of the objectives of their study of subject, the majority was to study with a partial knowledge of the objectives of their study. As to the plan of study, a low percentage indicated management of routine life under regular scheduling. Students of the 4th grade responded to rather planned life. As to time spent in independent study, response to concentrated study when necessary was stronger than that to regular daily study. Students of the 4th grade showed stronger response to regular study than that of the 3rd grade. As to the contents of their note-taking, 67.4 percents of those students responded to such regulatory procedure performing in the lecture-hall as they listen to lectures. 17.3 percents of those students showed response to adding supplementary informations from references to what was entered in choir note-taking at their lecture-halls. 6. Particulars of library, references and reading books: As to receiving of instruction for the utilization of library and time of receiving such instruction 64.7 percents of those students had received such instruction. 66.7 percents of the those responded received such instruction at orientation conducted for freshmen. As to the convenience of the utilization of library, 49.9 percents of those students responded to convenience. However, students of the 3rd grade showed a much stronger response to inconvenience. As to the time of the utilization of library,92.5 percents of those students showed a response to occasional utilization for particular purpose than regular utilization. 53.2 percents of those students responded to ordinary in quantity that library have references. 34.2 percents of those students responded to insufficient. As to the particular relative with the method and field of reading: 53.5 percents of those students responded to intensive reading and was the majority. As to the reading field, fiction u as the majority. When read any books for their major, they usually rend Korean text-b, oks. 7. Particular relative with giving assignment: All respondents were well aware of the objectives of giving home tasks. As to the attitude toward assignments and performing home tasks, 54.8 percents of those students to making ostentatious study because of an excessive quantity of assignments imposed. For performing assignment, they showed comparatively positive response. Also, 52.2 percents of those students responded that they usually submitted complected assignment with references. 8. As to motive to realize the necessity of study : 55.6 percents of those students responded that they realized such necessity in communication with patients when they were engaged in clinical practice. Also, 8.6, the lowest percents of those students responded that they realized such necessity in the course of conversation with nurses when they were engaged in clinical practice. 9. As to the determination of their confidence in the performance of study relative with clinical experience: They showed a general inclination of having in nursing. The major response was that they came to well comprehend the patients families. the lowest response was that they could apply what was learned at lecture-hall to practice. This response incidentally showed the distance the lecture-hall and practical study. In general items, students of the 4th grade showed more favorable response than students of the 3rd grade and there was a significant difference. 10. As to the perception and attitude toward profession according to the motive of selecting the nursing science : Those who selected the nursing voluntarily showed stronger conception than those who selected the nursing through indirect influence. However, there was no significant difference on this point. Only there was a remarkable difference in the reading of Code for Nurses. 11. Those who showed a stronger conception in the profession of nursing according to the motives of attractive nursing science indicated a strong will and ability to manage stable life and comparatively strong response was shown in the management of good home life because of the good adaptability of the science to their character. This group showed a strong conception of the profession than those who responded that they prefer this profession out of a longing for the work of a hospital and for the easy obtaining of opportunity to immigrate to over seas and for economic cause and for high school grade. There was significant difference between these two groups, 12. As to the conception and attitude toward the profession of nursing according to benefits by scholarships, those who were benefitted by scholarship showed stronger conception of profession than those who did not receive scholarship and there was a remarkable difference between these two categories. However, there was no remarkable difference between these two categories in the extent of interest of the activities of nursing fields and in the reading of Code for Nurses. 13. As activation for study according to the benefits of scholarships, those who were benefitted by scholarships showed stronger response to the motive for study comparing with those who receive. 14. As to tile field of reading according to the benefits by scholarships, those who received scholarships tended to read autobiographies and essayers to a considerable extent. Those who did not receive scholarships tended to read novels. Those who received scholarships more read nursing boots than those who did not receive scholarships. 15. As to the attitude of study and doing of assignment according to benefits of scholarships, those who received scholarships managed a favorable life with schedules for study, More students of receiving scholarship showed a regular study for more than one hour per day. Also, in the method of doing home tasks, more students of receiving scholarship showed reference to relative books frequently for the submission of completed assignments.

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학교도서관의 정보서비스 전개 방향과 활성화 전략 (Progress and Activation Strategies of Information Service in the School Library)

  • 송기호
    • 한국비블리아학회지
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    • 제24권2호
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    • pp.49-69
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    • 2013
  • 본 연구의 목적은 학교도서관 정보서비스의 범주를 설정하여 정보서비스 현황을 분석하고 활성화 전략을 제안하는 것이다. 학교도서관의 정보서비스 범위와 내용 설정 결과 정보제공 영역과 안내 영역의 서비스 내용이 독서교육이나 교과와 연계한 정보활용교육과정 운영으로 수렴되고 있는 것으로 나타났다. 그러나 2012전국도서관운영평가 결과 학교도서관의 정보서비스 도달률은 48.2%로 매우 낮게 나타났다. 특히 독서상담이나 독서치료 등 학생의 자아탄력성과 학교 참여를 높일 수 있는 정의적 영역과 관련된 정보서비스 내용이 매우 열악한 실정이다. 따라서 학교도서관의 정보서비스 활성화를 위해서는 사서교사 등 전문 인력자원의 지속적인 배치를 위한 제도 개선이 절실한 실정이다. 또한 학교도서관 정보서비스의 핵심 영역인 정보활용교육의 국가 수준 교육과정에서의 위상 강화, 정보서비스에 필요한 기반 확충 노력이 지속적으로 이루어질 필요가 있다. 아울러 독서상담과 치료 등 학생의 스트레스와 문제 상황을 해결하는데 기여할 수 있는 서비스 개발이 필요하다. 그리고 학교도서관 정보서비스가 지역사회와 연계성을 가짐으로써 학교 공동체 활성화에 기여해야 할 것으로 보인다.

Big6 모델 및 수정 모델 분석 연구 (Analysis of the Big6 Skills Model and the Modified Big6 Models)

  • 박주현
    • 한국도서관정보학회지
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    • 제49권3호
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    • pp.331-359
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    • 2018
  • 이 연구의 목적은 Big6 모델과 Big6 수정 모델을 분석하여 Big6 모델의 특징을 찾고 현장에서 Big6 모델을 적용하는데 필요한 시사점을 도출하는 데 있다. 이를 위하여 AASL과 ACRL의 정보 리터러시 기준과 Big6 모델을 비교하였으며, 교육목표분류학에 영향을 받은 Big6 모델과 Big6+3 모델, Big8 모델 및 LG사이언스랜드에서 제공하는 Big6 모델을 분석하였다. 분석결과, Big6 모델은 정보 문제 해결 모델과 메타인지 활성화 전략 및 학생들의 정보 리터러시를 향상시키는 발판으로 활용이 가능하였으며 구성주의, 탐구기반 학습, 교육과정 통합, 협력교육, ICT기술 모델로 활용이 가능하였다. 비판적 사고능력 향상은 Big6 모델보다 사서교사나 사서의 Big6 모델의 적용방법과 관련이 있었다. 사서교사와 사서는 Big6 모델을 적용하기 위하여 교육과정을 체계적이고 구체적으로 계획할 필요가 있다.

A Novel Esterase from Paenibacillus sp. PBS-2 Is a New Member of the ${\beta}$-Lactamase Belonging to the Family VIII Lipases/Esterases

  • Kim, Young-Ok;Park, In-Suk;Nam, Bo-Hye;Kim, Dong-Gyun;Jee, Young-Ju;Lee, Sang-Jun;An, Cheul-Min
    • Journal of Microbiology and Biotechnology
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    • 제24권9호
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    • pp.1260-1268
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    • 2014
  • Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and 42% identity with the putative ${\beta}$-lactamases from Paenibacillus sp. JDR-2 and Clostridium sp. BNL1100, respectively. The esterase contained a Ser-x-x-Lys motif that is conserved among all ${\beta}$-lactamases found to date. The protein PBS-2 was produced in both soluble and insoluble forms when E. coli cells harboring the gene were cultured at $18^{\circ}C$. The enzyme is a serine protein and was active against p-nitrophenyl esters of $C_2$, $C_4$, $C_8$, and $C_{10}$. The optimum pH and temperature for enzyme activity were pH 9.0 and $30^{\circ}C$, respectively. Relative activity of 55% remained at up to $5^{\circ}C$ with an activation energy of 5.84 kcal/mol, which indicates that the enzyme is cold-adapted. Enzyme activity was inhibited by $Cd^{2+}$, $Cu^{2+}$, and $Hg^{2+}$ ions. As expected for a serine esterase, activity was inhibited by phenylmethylsulfonyl fluoride. The enzyme was remarkably active and stable in the presence of commercial detergents and organic solvents. This cold-adapted esterase has potential as a biocatalyst and detergent additive for use at low temperatures.

SNS를 활용한 학교도서관 마케팅 활성화 방안 연구 (A Study of the Use of SNS for the Activation of School Library Marketing)

  • 임정훈
    • 한국도서관정보학회지
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    • 제51권1호
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    • pp.21-41
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    • 2020
  • 본 연구는 학교도서관의 SNS 마케팅 활용사례를 분석하여, 학교도서관 현장에 SNS 마케팅 활성화 방안을 제안하는 데 목적이 있다. 이를 위해 SNS를 학교도서관 마케팅에 활용하는 사서교사 10명과 심층면담을 실시하였고, 학교도서관 SNS 마케팅 운영 현황, 계기 및 목적, 전략, 성과를 분석하였다. 연구 결과, 사서교사들은 페이스북, 인스타그램, 카카오 플러스 친구 등의 SNS 서비스를 이용하는 것으로 조사되었다. 대부분 학교도서관 활성화를 고민하는 과정에서 SNS를 활용하게 된 것으로 나타났으며, 방문자 증가, 이용자의 요구 파악 등 학교도서관 활용 증대 및 이용자와 소통을 목적으로 SNS를 이용하는 것으로 확인되었다. SNS를 통해 제공되는 메시지는 학교도서관 이용안내, 독서 프로그램 홍보 등이며, 마케팅의 대상은 주로 학생으로 파악되었다. 이상의 연구 결과를 토대로 마케팅 협의체의 필요성, SNS 마케팅 대상의 확장, SNS 활용에 대한 전문성 신장 등 학교도서관 SNS 마케팅 활성화 방안을 제안하였다.

사서교사의 맞춤형 심화연수 프로그램용 연수과목에 대한 선호도 분석 (An Analysis of Teacher Librarians' Preference on Subjects for their Customized Intensive In-Service Training Program)

  • 송기호
    • 한국문헌정보학회지
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    • 제45권2호
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    • pp.163-184
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    • 2011
  • 본 연구의 목적은 사서교사의 교원능력개발 평가지표별 맞춤형 심화연수 프로그램용 연수과목에 대한 선호도를 분석하여 성공적인 현직연수 설계를 위한 기초자료를 제공하는 것이다. 설문 분석 결과 사서교사들은 독서교육, 정보활용교육과 같이 교육정보봉사 영역과 관련된 연수과목을 핵심 업무로 인식하고 있으며, 교육용 콘텐츠 개발과 수업용 자료 개발에 대한 선호도가 높은 것으로 나타났다. 학교급별로는 초등학교 사서교사가 도서관 활성화 프로그램, 자원봉사자 관리, 이용자 요구 및 교육과정 분석, 이용자 교육 평가 그리고 정보활용교육 평가를 선호하는 것으로 나타났다. 특히 연령이 높을수록 메타 데이터에 대한 이해, 정보시스템 구축 그리고 정보시스템과 교육지원에 대한 선호도가 높았다. 따라서 사서교사 연수과목은 학교도서관 교육과 교육과정 연계 방법을 중심으로 구성하고, 연수 방법은 이론 중심의 강의보다는 사례 연구와 개발 중심으로 이루어지는 것이 바람직하다.

Isolation and expression analysis of stimulator of interferon gene from olive flounder, Paralichthys olivaceus

  • Ma, Jeong-In;Kang, Sunhye;Jeong, Hyung-Bok;Lee, Jehee
    • Fisheries and Aquatic Sciences
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    • 제21권3호
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    • pp.5.1-5.8
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    • 2018
  • Stimulator of interferon gene (STING) is induced by various inflammatory agents, such as lipopolysaccharide and microbial pathogens, including virus and bacteria. In this study, we obtained a full-length cDNA of a STING homolog from olive flounder using rapid amplification of cDNA ends PCR technique. The full-length cDNA of Paralichthys olivaceus STING (PoSTING) was 1442 bp in length and contained a 1209-bp open reading frame that translated into 402 amino acids. The theoretical molecular mass of the predicted protein sequence was 45.09 kDa. In the PoSTING protein, three transmembrane domains and the STING superfamily domain were identified as characteristic features. Quantitative real-time PCR revealed that PoSTING expressed in all the tissues analyzed, but showed the highest level in the spleen. Temporal expression analysis examined the significantly upregulated expression of PoSTING mRNA after viral hemorrhagic septicemia virus (VHSV) stimulation. In contrast, no significant changes in the PoSTING expression were detected in Edwardsiella tarda-challenged group compared to the un-injected control. The expression of P. olivaceus type I interferon (PoIFN-I) was also highly upregulated upon VHSV challenge. These results suggest that STING might be involved in the essential immune defense against viral infection together with the activation of IFN-I in olive flounder.

ZNF435, a Novel Human SCAN-containing Zinc Finger Protein, Inhibits AP-1-mediated Transcriptional Activation

  • Gu, Xing;Zheng, Mei;Fei, Xiangwei;Yang, Zhenxing;Li, Fan;Ji, Chaoneng;Xie, Yi;Mao, Yumin
    • Molecules and Cells
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    • 제23권3호
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    • pp.316-322
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    • 2007
  • Zinc finger transcription factor genes are a significant fraction of the genes in the vertebrate genome. Here we report the isolation and characterization of a human zinc finger-containing gene, ZNF435, from a fetal brain cDNA library. ZNF435 cDNA is 1290 base pairs in length and contains an open reading frame encoding 349 amino acids with four C2H2-type zinc fingers at its carboxyl terminus and a SCAN motif at its amino terminus. RT-PCR results showed that ZNF435 was expressed in all tested tissues. A ZNF435-GFP fusion protein was located in the nucleus and the four zinc fingers acted as nuclear localization signals (NLSs). ZNF435 was found to be capable of homo-association, and this effect was independent of its zinc fingers. Furthermore, ZNF435 proved to be a transcription repressor as its overexpression in AD293 cells inhibited the transcriptional activities of AP-1.

지역사회 특성을 반영한 공공도서관 활성화 방안 연구 - 경기도 양평군을 중심으로 - (Research on Revitalizing Public Libraries Reflecting Community Characteristics in Yangpyeong-gun)

  • 전경선;신동희
    • 한국비블리아학회지
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    • 제33권1호
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    • pp.403-423
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    • 2022
  • 본 연구는 양평군 지역사회의 특성을 반영한 공공도서관의 운영 개선 및 활성화 방안을 제시하는 데 목적이 있다. 이를 위해 양평군 지역특성 및 공공도서관 운영 현황을 분석하였으며, 양평군민(도서관 이용자 및 비이용자)을 대상으로 설문조사를 실시하였다. 또한 도서관의 운영 현황 및 한계점, 도서관 활성화 방안에 대한 사서들의 인식조사를 위하여 인터뷰를 수행하였다. 분석 결과, 양평군은 도서관 접근성 취약, 인력 및 장서 부족, 지역특성을 반영하지 못한 프로그램, 도서관 공간 부족 및 건물의 노후화 등이 주요 문제점으로 도출되었다. 이에 본 연구에서는 접근성 개선을 위한 지역중심의 생활밀착형 도서관, 인력 및 장서 확충, 지역사회 변화를 반영한 프로그램 개발, 신규 도서관 건립을 통한 복합문화공간 제공 등의 활성화 방안을 제시하였다.