• The Plant Pathology Journal
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• v.27 no.2
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• pp.148-155
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• 2011

Our previous sequence and phylogenetic analyses of the Korean Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSV-RNA segment variations, we developed a reverse transcription-polymerase reaction/restriction enzyme (RT-PCR/RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that the migratory small brown planthopper from Eastern China caught by aerial net traps that possesses RSV-RNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/RE, sequence and phylogenetic analyses, indicating that RSV populations in Eastern China may also have strong RNA variation. The development of an RE analysisbased method proved a useful epidemiological tool for rapid genotyping and identification of mixed infections by RSV strain and by different subtype.

• Journal of Ginseng Research
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• v.41 no.1
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• pp.36-42
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• 2017

Background: Some differences have been reported in the biotransformation of ginsenosides, probably due to the types of materials used such as ginseng, enzymes, and microorganisms. Moreover, most microorganisms used for transforming ginsenosides do not meet food-grade standards. We investigated the statistical conversion rate of major ginsenosides in ginsenosides model culture during fermentation by lactic acid bacteria (LAB) to estimate possible pathways. Methods: Ginsenosides standard mix was used as a model culture to facilitate clear identification of the metabolic changes. Changes in eight ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, and Rg2) during fermentation with six strains of LAB were investigated. Results: In most cases, the residual ginsenoside level decreased by 5.9-36.8% compared with the initial ginsenoside level. Ginsenosides Rb1, Rb2, Rc, and Re continuously decreased during fermentation. By contrast, Rd was maintained or slightly increased after 1 d of fermentation. Rg1 and Rg2 reached their lowest values after 1-2 d of fermentation, and then began to increase gradually. The conversion of Rd, Rg1, and Rg2 into smaller deglycosylated forms was more rapid than that of Rd from Rb1, Rb2, and Rc, as well as that of Rg1 and Rg2 from Re during the first 2 d of fermentation with LAB. Conclusion: Ginsenosides Rb1, Rb2, Rc, and Re continuously decreased, whereas ginsenosides Rd, Rg1, and Rg2 increased after 1-2 d of fermentation. This study may provide new insights into the metabolism of ginsenosides and can clarify the metabolic changes in ginsenosides biotransformed by LAB.

• Nuclear Engineering and Technology
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• v.53 no.11
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• pp.3790-3797
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• 2021

Although the individual channel readout method can improve the performance of PET detectors with pixelated photo-sensors, such as silicon photomultiplier (SiPM), this method leads to a significant increase in the number of readout channels. In this study, we proposed a novel multiplexing method that could effectively reduce the number of readout channels to reduce system complexity and development cost. The proposed multiplexing circuit was designed to generate bipolar pulses with different zero-crossing points by adjusting the time constant of the high-pass filter connected to each channel of a pixelated photo-sensor. The channel position of the detected gamma-ray was identified by estimating the width between the rising edge and the zero-crossing point of the bipolar pulse. In order to evaluate the performance of the proposed multiplexing circuit, four detector blocks, each consisting of a 4 × 4 array of 3 mm × 3 mm × 20 mm LYSO and a 4 × 4 SiPM array, were constructed. The average energy resolution was 13.2 ± 1.1% for all 64 crystal pixels and each pixel position was accurately identified. A coincidence timing resolution was 580 ± 12 ps. The experimental results indicated that the novel multiplexing method proposed in this study is able to effectively reduce the number of readout channels while maintaining accurate position identification with good energy and timing performance. In addition, it could be useful for the development of PET systems consisting of a large number of pixelated detectors.

• Natural Product Sciences
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• v.21 no.2
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• pp.111-121
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• 2015

The root of Panax ginseng, is a Korea traditional medicine, which is used in both raw and processed forms due to their different pharmacological activities. As part of a continued chemical investigation of ginseng, the focus of this research is on the isolation and identification of compounds from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, semi-preparative-high performance liquid chromatography, Fast atom bombardment mass spectrometric, and nuclear magnetic resonance. Dammarane-type triterpenoid saponins were isolated from Panax ginseng root by open column chromatography, medium pressure liquid chromatography, and semi-preparative-high performance liquid chromatography. Their structures were identified as protopanaxadiol ginsenosides [gypenoside-V (1), ginsenosides-Rb1 (2), -Rb2 (3), -Rb3 (4), -Rc (5), and -Rd (6)], protopanaxatriol ginsenosides [20(S)-notoginsenoside-R2 (7), notoginsenoside-Rt (8), 20(S)-O-glucoginsenoside-Rf (9), 6-O-[$\alpha$-L-rhamnopyranosyl(1$\rightarrow$2-$\beta$-D-glucopyranosyl]-20-O-$\beta$-D-glucopyranosyl-$3\beta$,$12\beta$, 20(S)-dihydroxy-dammar-25-en-24-one (10), majoroside-F6 (11), pseudoginsenoside-Rt3 (12), ginsenosides-Re (13), -Re5 (14), -Rf (15), -Rg1 (16), -Rg2 (17), and -Rh1 (18), and vinaginsenoside-R15 (19)], and oleanene ginsenosides [calenduloside-B (20) and ginsenoside-Ro (21)] through the interpretation of spectroscopic analysis. The configuration of the sugar linkages in each saponin was established on the basic of chemical and spectroscopic data. Among them, compounds 1, 8, 10, 11, 12, 19, and 20 were isolated for the first time from P. ginseng root.

• Journal of Ginseng Research
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• v.44 no.6
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• pp.757-769
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• 2020

Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius. UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.6 no.7
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• pp.9-16
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• 2016

As life in a rapidly changing Internet age at home and abroad, large amounts of information are being used medical, financial, services, etc. Accordingly, especially hospitals, is an invasion of privacy caused by leakage and intrusion of personal information in the system in medical institutions, including clinics institutions. To protect the privacy & information protection of personal health medical information in medical institutions at home and abroad presented by national policies and de-identification processing technology standards in accordance with the legislation. By comparative analysis in existing domestic and foreign institutional privacy and de-identification technique, derive a advanced one of pseudonymization and anonymization techniques for destination data items that fell short in comparison to the domestic laws and regulations, etc. De-identification processing technology for personal health information is compared to a foreign country pharmaceutical situations. We propose a new de-identification techniques by reducing the risk of re-identification processing to enable the secondary use of domestic medical privacy.

• Communications for Statistical Applications and Methods
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• v.22 no.6
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• pp.575-587
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• 2015

The development in data collection techniques results in high dimensional data sets, where discrimination is an important and commonly encountered problem that are crucial to resolve when high dimensional data is heterogeneous (non-common variance covariance structure for classes). An example of this is to classify microbial habitat preferences based on codon/bi-codon usage. Habitat preference is important to study for evolutionary genetic relationships and may help industry produce specific enzymes. Most classification procedures assume homogeneity (common variance covariance structure for all classes), which is not guaranteed in most high dimensional data sets. We have introduced regularized elimination in partial least square coupled with QDA (rePLS-QDA) for the parsimonious variable selection and classification of high dimensional heterogeneous data sets based on recently introduced regularized elimination for variable selection in partial least square (rePLS) and heterogeneous classification procedure quadratic discriminant analysis (QDA). A comparison of proposed and existing methods is conducted over the simulated data set; in addition, the proposed procedure is implemented to classify microbial habitat preferences by their codon/bi-codon usage. Five bacterial habitats (Aquatic, Host Associated, Multiple, Specialized and Terrestrial) are modeled. The classification accuracy of each habitat is satisfactory and ranges from 89.1% to 100% on test data. Interesting codon/bi-codons usage, their mutual interactions influential for respective habitat preference are identified. The proposed method also produced results that concurred with known biological characteristics that will help researchers better understand divergence of species.

• Journal of Biomedical Engineering Research
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• v.30 no.4
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• pp.279-287
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• 2009

Magnetic resonance electrical impedance tomography (MREIT) is a new medical imaging modality providing cross-sectional images of a conductivity distribution inside an electrically conducting object. MREIT has rapidly progressed in its theory, algorithm and experimental technique and now reached the stage of in vivo animal and human experiments. Conductivity image reconstructions in MREIT require various steps of carefully implemented numerical computations. To facilitate MREIT research, there is a pressing need for an MREIT software package with an efficient user interface. In this paper, we present an example of such a software, called CoReHA which stands for conductivity reconstructor using harmonic algorithms. It offers various computational tools including preprocessing of MREIT data, identification of boundary geometry, electrode modeling, meshing and implementation of the finite element method. Conductivity image reconstruction methods based on the harmonic $B_z$ algorithm are used to produce cross-sectional conductivity images. After summarizing basics of MREIT theory and experimental method, we describe technical details of each data processing task for conductivity image reconstructions. We pay attention to pitfalls and cautions in their numerical implementations. The presented software will be useful to researchers in the field of MREIT for simulation as well as experimental studies.

• Journal of the Earthquake Engineering Society of Korea
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• v.6 no.6
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• pp.7-15
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• 2002

In this paper, the nonlinear parameter estimation method using the estimated hysteresis of each structural members was studied for the purpose of efficient seismic damage prediction and estimation of MDOF nonlinear structural model in the shaking table test. The hysteresis of each structural members can be obtained by the conversion of measured response histories into relative motions of each structural members and member forces. These hysteresis can be used to evaluate various kinds of damage indices of each structural members. The MDOF nonlinear structural model for further analysis(re-analysis) can be easily reconstructed using estimated nonlinear structural parameters of each structural members. To demonstrate the proposed techniques, several numerical and experimental example analyses are carried out. The results indicate that the proposed method can be very useful to assess local seismic damages of structures.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.40-40
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• 2017

Rice (Oryza sativa L.) is one of major crops of Vietnam. Rice plant contains many secondary metabolites that are potent to inhibit growth of other plants. This study was carried out to find potential plant growth inhibitors from rice plants. Seven rice varieties were cultivated in the fields affiliated to Hiroshima University, Japan; Mature stems and leaves from each variety were collected, and then they were extracted with methanol, hexane, ethyl acetate, butanol, and water. Total phenolic content and total flavonoid content were the highest in ethyl acetate extract. DPPH antioxidant assay results showed that the ethyl acetate extract also had higher $IC_{50}$ value. Therefore, the ethyl acetate extracts were selected for laboratory bioassay. Results showed that the two-local variety Re nuoc and Nan chon inhibited the germination of radish and barnyard grass seedlings at the greatest levels, as compared to other cultivars. HPLC quantification indicated that the ethyl acetate extracts of Re nuoc and Nan chon rice plant parts consists of phenolic and flavonoids compounds which are potent as plant growth inhibitors. Further laboratory bioassay and field experimentation will be conducted to validate the laboratory bioassay findings.