• Title/Summary/Keyword: Re-Identification

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Isolation and Structural Identification of Antioxidant Substances from Ethyl Acetate Extract of Conyza canadensis (망초(Conyza canadensis) Ethyl Acetate 추출물의 항산화성 물질의 분리와 동정)

  • Hyun Sook Song
    • Journal of Naturopathy
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    • v.12 no.1
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    • pp.7-15
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    • 2023
  • Background: As a result of analyzing the components of wild Conyza canadensis, it contains physiologically active ingredients, so it is necessary to identify the compound. Purposes: It was to study the compound's molecular structure; a previous study showed that C. canadensis contains antioxidant substances. Methods: The ultrasonic pulverized lysate of C. canadensis stem and leaves was first extracted with 90% methanol and then five organic solvents. Next, the extracts was fractionated by HPLC, LC/MS chromatography, and NMR analyzers identified the molecular structure. Results: 100 g of dry C. canadensis was sonicated in 90% methanol and concentrated under reduced pressure to 11.96 g of a crude extract. Then, this crude was extracted with five types of solvents to obtain 123.8 mg of n-hexane, 448.2 mg of dichloromethane, 1047.7 mg of ethyl acetate (EA), 2563.8 mg of butanol, and 7.04 g of water. The EA extracts were fractionated by LC-MS and then re-fractionated to obtain F1 to F20. Next, the F15 was further fractionated to obtain nine fine fractions. Finally, the F17 fraction was re-fractionated to obtain ten fine fractions. As a result of LC-MS and NMR spectrometer analysis of the F15-7, the structure of this compound was confirmed as 3,5-dicaffeoylquinic acid. As a result of examining the structures of the F17-4 and F17-5 fractions, Quercetin-3-o-β-galactose was identified. In addition, the form of the F17-10 was confirmed to be 1,3,4-tri-caffeoylquinic acid. Conclusions: This study demonstrated that C. canadensis contained phenolic antioxidants, and its utilization may be expected.

A Study on the Link Server Development Using B-Tree Structure in the Big Data Environment (빅데이터 환경에서의 B-tree 구조 기반 링크정보 관리서버의 개발)

  • Park, Sungbum;Hwang, Jong Sung;Lee, Sangwon
    • Journal of Internet Computing and Services
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    • v.16 no.1
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    • pp.75-82
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    • 2015
  • Major corporations and portals have implemented a link server that connects Content Management Systems (CMS) to the physical address of content in a database (DB) to support efficient content use in web-based environments. In particular, a link server automatically connects the physical address of content in a DB to the content URL shown through a web browser screen, and re-connects the URL and the physical address when either is modified. In recent years, the number of users of digital content over the web has increased significantly because of the advent of the Big Data environment, which has also increased the number of link validity checks that should be performed in a CMS and a link server. If the link validity check is performed through an existing URL-based sequential method instead of petabyte or even etabyte environments, the identification rate of dead links decreases because of the degradation of validity check performance; moreover, frequent link checks add a large amount of workload to the DB. Hence, this study is aimed at providing a link server that can recognize URL link deletion or addition through analysis on the B-tree-based Information Identifier count per interval based on a large amount of URLs in order to resolve the existing problems. Through this study, the dead link check that is faster and adds lower loads than the existing method can be performed.

Implementation of an Efficient Microbial Medical Image Retrieval System Applying Knowledge Databases (지식 데이타베이스를 적용한 효율적인 세균 의료영상 검색 시스템의 구현)

  • Shin Yong Won;Koo Bong Oh
    • Journal of the Korea Society of Computer and Information
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    • v.10 no.1 s.33
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    • pp.93-100
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    • 2005
  • This study is to desist and implement an efficient microbial medical image retrieval system based on knowledge and content of them which can make use of more accurate decision on colony as doll as efficient education for new techicians. For this. re first address overall inference to set up flexible search path using rule-base in order U redure time required original microbial identification by searching the fastest path of microbial identification phase based on heuristics knowledge. Next, we propose a color ffature gfraction mtU, which is able to extract color feature vectors of visual contents from a inn microbial image based on especially bacteria image using HSV color model. In addition, for better retrieval performance based on large microbial databases, we present an integrated indexing technique that combines with B+-tree for indexing simple attributes, inverted file structure for text medical keywords list, and scan-based filtering method for high dimensional color feature vectors. Finally. the implemented system shows the possibility to manage and retrieve the complex microbial images using knowledge and visual contents itself effectively. We expect to decrease rapidly Loaming time for elementary technicians by tell organizing knowledge of clinical fields through proposed system.

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Research on Ginseng Production During the Past 20 years (인삼재배 분야의 과거 20년 연구)

  • Park, Hoon
    • Journal of Ginseng Research
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    • v.20 no.4
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    • pp.472-500
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    • 1996
  • Researches on mineral nutrition, physiology and phyrsiological diseases, . cultivaction methods. brceding. pest control quality management and extension during 1976-1995 in Korea were reviewed Review in brceding and pest control was restricted to the researches directely related to cultivaction. Mineral nulrient up take. partion and varicos factors such as top dreasing. Light intersity etc. and interrelationship between minerals were investigated. Top dressing was not effective due to low minera1 requorement Physiological characteristics on tempelature light and water were well elucidated and applied to assess traditional cultivation method and its inovation. Photosyrnthetic pigments. light harvest proteins and activity of related enzymes were studied. In nitrogen metabolism arginine, praline, ammonium, threonine appeared to have important role in re growth of shoot Saponin metabolism was studied in relation to growth and new ginsenosides were found but physiological role of saponin was not clearly elucidated yet Endogenous growth regulators were reported and various erogenous growth regulators were studied for growth stimulation. short stem and seed pruning etc. Various physiological diseases were investigated for cause and control measures were established. Water culture was little studied Forest culture was studied but not retched the recommendable stage Drip irrigation straw mulching. seasonal shading and soil preparation method including soil fertility adjustment were established for practical application. Shading materials completely changed to polyethylene net and materials of polymers The research on ginseng cultivation in paddy field opened the way to establish the permanent ginseng cultivation plantation Ginseng harvester and seeder were developed in the late 1950s. Transplanted and many other machines were developed in the early 1990s. In ginseng breeding only pure line selection was of practical significance several verities were at the stage of seed propagation at ginseng plantations. Mutation breeding (${\gamma}$-ray. X-ray chemicals) was not successful. The research on plantlet formation through tissue culture was a little progressed but still far behind to vegetative propagation. Disease control research was concentrated in the isolation and identification of pathogans. their ecological charactelistics and biological control and soil humigation. Potato root rot nematodes was found and control method was established. Insect and small animal control research was greatly progresses in identification, ecological investigation, and ecological and physical control. Weed control was less important due to the development of mulching method of ridge and ditch. Quality factors of raw ginseng in relation to red ginseng process were extensively studied. Traditional quality measures were elucidated in accordance with modern analytical chemistry resulting in the importance of peptides in the centrat part rather than ginsenosides For large root production growth promoting rootzone micrcorganisms (PGPRM) were isolated and active compounds were identified. Field test on PGPRM was on going. Varictus methods formality improvement through cultivation were developed. Management research of ginseng production was rare Extension was active throuch official and private organizations and through workshop for the extension specialists, and direct lectures to grower's. Extension services made the researcher to understand the existing problems at grower's fields. Research environment for ginseng production was in prime time only for three years when Korea Ginseng Research Institute was established then gradually aggravated.

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Indirect Anthropometry on Cast Model of Cleft Lip Nose: Comparison with Direct Anthropometry (구순열비 석고모형에서 간접인체계측법: 직접인체계측법과의 비교)

  • Han, Ki Hwan;Jeong, Hoi Joon;Jin, Hyun Seok;Kim, Jun Hyung;Son, Dae Gu
    • Archives of Plastic Surgery
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    • v.34 no.1
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    • pp.18-23
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    • 2007
  • Purpose: Anthropometry can be divided into two methods, direct anthropometry and indirect anthropometry. The most ideal and accurate method is a direct anthropometry. However, it is difficult to measure in the case of children because of poor cooperation, and it lacks re-productivity. Cast model has advantages of three dimensional featuring, inexpensive and easy fabrication. This study is conducted to find out an accuracy of indirect anthropometry on cast model by comparing it with direct anthropometry. Methods: Total 48 cleft lip nasal deformity patients (unilateral, 40; bilateral, 8) were included in this study. Cast models were made before surgery under general anesthesia with alginate impression material and model plaster. Eleven linear measurements among 7 landmarks were taken as direct anthropometry before surgery with Castroviejo spreading caliper. At the same time, indirect anthropometry on cast model was done at the same linear distances as well. Results: Of the total 11 linear measurements, both ala lengths, both columella lengths, nose width, projective distance between facial insertion points of the ala, projective distance between the alar base points, right nostril floor width, and columella width were statistically correlated between indirect anthropometry on cast model and direct anthropometry. However, the nasal tip protrusion and the left nostril floor width were not statistically correlated. Conclusion: Accuracy of indirect anthropometry on cast model can be influenced by cast model fabrication techniques and correct identification of landmarks. Nasal tip protrusion could be reduced by compression of the nasal tip in the process of cast model fabrication and nostril floor width can be varied by muscle relaxation of anesthetics and incorrect identification of subalare in cleft lip nasal deformity. If sufficient care is taken to make cast model and to define landmarks exactly, indirect anthropometry on cast model can be a reliable method as direct anthropometry.

Frequency-Scanning Type Microwave Tag System Using Defected Ground Structures (결함 접지 구조를 이용한 주파수 스캐닝 방식의 마이크로파 태그 시스템)

  • Lee, Seok-Jae;Han, Sang-Min
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.24 no.3
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    • pp.247-252
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    • 2013
  • In this paper, a microwave tag system of a frequency-scanning type is proposed with multi-resonators using defected ground structures. While a conventional chip-based RFID stores time-sequential codes, the proposed type achieves pure passive tags by using multi-resonant bits over a frequency range. Moreover, the resonators of the spiral defected ground structures implemented on the back side of transmission lines have advantages of the excellent bandstop characteristics as well as the bit-error avoidance by the re-radiation on normal resonators. The proposed microwave tag is designed with UWB antennas at 3~7 GHz. From the experimental results in an anechoic chamber, it has been verified of the excellent recognitions for various 5-bits identification codes.

Improving Personal Data Protection in IoT Environments (사물인터넷(IoT) 환경에서 개인정보보호 강화를 위한 제도 개선 방안)

  • Lee, Ae Ri;Son, Soomin;Kim, Hyun Jin;Kim, Beomsoo
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.26 no.4
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    • pp.995-1012
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    • 2016
  • In Internet of Things (IoT) environments, devices or sensors everywhere can automatically collect data without the individual awareness, further combine and share data using ubiquitous network, and thus the development of IoT raises new challenges in respect of personal data protection and privacy. This study aims to identify main issues related to data protection in the IoT and propose adequate measures. We analyzed the types of personal data controllers and processors in IoT and figured out the issues regarding the processing of personal data and the rights to privacy of data subject. Accordingly, we suggested the institutional ways (e.g., establishment of user-friendly notice and flexible consent system, re-identification risk monitoring system, data protection in cross-border transfer, and user education) to improve the situation of personal data protection in IoT and finally proposed the improvement tasks to carry out first based on the degree of urgency and importance.

Strengthening Families: The Re-structuring of Family Services in Hong Kong

  • Leung, Joe C.B.
    • 한국사회복지학회:학술대회논문집
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    • 2002.10a
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    • pp.3-19
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    • 2002
  • The problems and needs of families in Hong Kong are becoming more complex. Traditional family structures and functions are rapidly eroding. Moreover, family problems are further exacerbated by a growing number of socio-economic issues including rising divorce rates and extra-marital affairs. continuous family reunion migration from Mainland China, emotional and financial cost of caring for the older people and the changing economy which has pushed more families into hardship. In effect, more and more families are becoming vulnerable to risk. Traditional family services in Hong Kong are varied in mode, operation and provision, often overlapping in service boundaries with other family-oriented programs. In many instances, family service centers are stretched far beyond their capacity. As a consequence, they become too reactive, remedial and casework dominated. The University of Hong Kong Consultant Team recommended that family service programs have to protect vulnerable families and strengthen family capacities to promote maximum independence. The direction of family services is summarized as: strengthening families; child-centered, family-focused and community-based. New integrative family service centers aim at attaining the principles of promoting accessibility to users with minimum physical, psychological and administrative barriers; early identification of needs and intervention before the further deterioration of problems; integration of services cutting across program boundaries, and partnership between service providers to achieve efficient and effective use of scarce resources. Under the new model, multi-skilled teams can respond more proactively to a wide range of social needs, rather than addressing needs in isolation. To demonstrate the effectiveness of the new model, the consultant team has been commissioned by the government to evaluate the performance of these two-year pilot projects. More importantly, a coherent and family-friendly social policy should be formulated to strengthen family capacity against family-related problems.

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Crown and Root Rot of Strawberry Caused by Neopestalotiopsis clavispora in Korea (Neopestalotiopsis clavispora에 의한 딸기 뿌리썩음병 한국 내 발생)

  • Park, Kyoungmi;Han, Inyoung;Lee, Seok-Min;Choi, Si-Lim;Kim, Min Chul;Lee, Heungsu
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.427-435
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    • 2019
  • The occurrence of the crown and root rot on strawberry (Fragaria×ananassa Duch.) has been reported in greenhouses in Sancheong and Hamyang, Gyeongnam province, Korea in June, 2019. The infected plants showed browning rot of the inner crown and root, causing delayed development, lack of growth, and poor rooting. The browning rot of the inner crown and root can sometimes lead to wilting and collapsing of plants. Fungi were isolated from the symptomatic root and crown. Based on the results of morphological and phylogenetic analyses, the causal agent of the disease was identified to be Neopestalotiopsis clavispora. The fungal isolates were then used for inoculation into strawberry plants to determine the causal agent of the crown and root rot as per Koch's postulates. The inoculated strawberry plants showed the same symptoms as the originally infected plants, and the fungal pathogen re-isolated from the lesions showed the same morphological characteristics as the original pathogen. This is the first report on the occurrence of crown and root rot on strawberry (Fragaria×ananassa Duch.) caused by N. clavispora in Korea.

Identification of a Novel PGE2 Regulated Gene in SNU1 Gastric Cancer Cell

  • Park, Min-Seon;Kim, Hong-Tae;Min, Byung-Re;Kimm, Ku-Chan;Nam, Myeong-Jin
    • BMB Reports
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    • v.33 no.2
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    • pp.184-187
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    • 2000
  • Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

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