• 한국콘텐츠학회논문지
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• 제6권12호
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• pp.128-135
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• 2006

RTLS(Real Time Location System) 는 사물에 RTLS 태그를 부착하여 사물의 정보와 위치 정보를 실시간으로 제공해 주는 시스템이다. 본 논문에서는 항만운영시스템의 성능을 개선하기 위하여 컨테이너의 위치 정보를 실시간으로 제공해 주는 RFID(Radio Frequency Identification) 기반의 RTLS을 활용하여 장치장의 효율적인 운영방안을 제안한다. 그룹기반의 하역 순서 시스템에서 같은 그룹의 컨테이너는 목적지 항만, 컨테이너 크기, 무게 등이 비슷한 특성을 가진다. 이를 위하여 ISO 18000-7에서 표준화된 RFID 태그의 메시지 포맷에서 미정의된 N 바이트의 파라미터를 이용하는 방안을 제안한다. 성능 분석 평가 결과, 그룹 기반 시스템은 장치장에서 트랜스퍼크레인의 재조작 비율을 줄이고, 항만 물류의 전체 리드 타임을 감소시킨다.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2106-2115
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• 2016

To identify the global effects of (p)ppGpp in the gram-positive bacterium Deinococcus radiodurans, which exhibits remarkable resistance to radiation and other stresses, RelA/SpoT homolog (RSHs) mutants were constructed by direct deletion mutagenesis. The results showed that RelA has both synthesis and hydrolysis domains of (p)ppGpp, whereas RelQ only synthesizes (p)ppGpp in D. radiodurans. The growth assay for mutants and complementation analysis revealed that deletion of relA and relQ sensitized the cells to $H_2O_2$, heat shock, and amino acid limitation. Comparative proteomic analysis revealed that the bifunctional RelA is involved in DNA repair, molecular chaperone functions, transcription, the tricarboxylic acid cycle, and metabolism, suggesting that relA maintains the cellular (p)ppGpp levels and plays a crucial role in oxidative resistance in D. radiodurans. The D. radiodurans relA and relQ genes are responsible for (p)ppGpp synthesis/hydrolysis and (p)ppGpp hydrolysis, respectively. (p)ppGpp integrates a general stress response with a targeted re-programming of gene regulation to allow bacteria to respond appropriately towards heat shock, oxidative stress, and starvation. This is the first identification of RelA and RelQ involvement in response to oxidative, heat shock, and starvation stresses in D. radiodurans, which further elucidates the remarkable resistance of this bacterium to stresses.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.381-389
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• 2019

Chlorella vulgaris was isolated from the Nile River, Qalubia Governorate, Egypt, for possible use in biodiesel production. BG-II nutrient growth media was used for isolation and laboratory growth. Identification was performed via 18S rRNA gene amplification, followed by sequencing. The alga was exposed to UV-C (254 nm) for 15, 30, and 45 s to improve dry weight accumulation and to increase the oil production. Daily measurements of dry weight ($g{\cdot}l^{-1}$) were performed; oil content and volumetric lipid productivity were also determined. UV-C exposure led to an increase in the volumetric lipid productivity by 27, 27.3, and $32.4mg{\cdot}l^{-1}{\cdot}d^{-1}$ with 15, 30, and 45 s, respectively, as compared with the control, which resulted in $18mg{\cdot}l^{-1}{\cdot}d^{-1}$. Of the examined mutants, the one with the highest productivity was re-irradiated by UV-C (254 nm) for 15, 30, 45, and 60 s. For 15 s of exposure time, the oil content increased to 34%, while it was 31% at 30 s; further, it decreased to 22% at 45 and 60 s exposures. The fatty acid methyl ester profile was 82.22% in the first mutant at 45 s, compared with the wild strain that contained a total of 66.01% of FAs. Furthermore, the highest levels of polyunsaturated fatty acid methyl ester were observed in the mutant exposed for 45 s, and it reached 11.41%, which reduced the cetane number to 71.3.

• 천문학회보
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• 제40권1호
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• pp.71.3-71.3
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• 2015

Deep UBVI and $H{\alpha}$ photometry of the ${\eta}$ Carina nebula, one of the brightest nebulae on the sky, was obtained with the CTIO 4m telescope and MOSAIC II CCD Camera to determine the initial mass function down to low-mass (~1 M¤) stars. We modified the spatial variation coefficients in transformation relations of the MOSAIC II CCD. From the cross-identification of optical sources with previous surveys in X-ray, near-infrared, and mid-infrared, a clear PMS sequence is revealed in the optical color-magnitude diagrams down to V=23 mag. Our previous SSO 1m UBVI data for Trumpler 14 (Tr 14) and Trumpler 16 (Tr 16) region, and additional SSO 1m UBVI data for Trumpler 15 (Tr 15) region were combined with the CTIO 4m data to re-examine the reddening law and distance of the young open clusters in the ${\eta}$ Carina nebula. From the new photometric data for Tr 15 region, we report that RV[=AV/E(B-V)], the total-to-selective extinction ratio, decreases from southern part of the nebula (Tr 14 and Tr 16) to northern part (Tr 15) in our field of view.

• 식물분류학회지
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• 제50권2호
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• pp.232-238
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• 2020

The identity of Dioscorea coreana (Prain & Burkill) R. Kunth is recognized during the re-identification process of Korean Dioscorea specimens. Given the relatively few pieces of information, including few descriptions and research papers, this species has been misidentified as D. tokoro, which has a similar leaf shape, but D. coreana is distinguished from D. tokoro by the absence of a pedicel in the male flower, the green color of the tepal, and the shapes of the fruit and seed. Thus, detailed descriptions, illustrations, and photographs of D. coreana and a key to the Korean Dioscorea, including this species, are presented.

• The Plant Pathology Journal
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• 제34권1호
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• pp.1-10
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• 2018

A previously unreported bacterial disease on chili pepper (Capsicum annuum L.) seedlings affecting as many as 4% of seedlings was observed in greenhouses in Chihuahua, Mexico (Delicias and Meoqui counties). Initial lesions appeared as irregular small spots on leaves and brown necrosis at margins tips were observed. Later, the spots became necrotic with a chlorotic halo. Advanced disease was associated with defoliation. A Gram negative, rod-shaped bacterium was isolated from diseased chili pepper seedlings. Three inoculation methods revealed that isolated strains produce foliage symptoms, similar to those observed in naturally infected seedlings. Pathogenic strains that caused symptoms in inoculated seedlings were re-isolated and identified to fulfill koch's postulate. Polyphasic approaches for identification including biochemical assays (API 20E and 50CH), carbon source utilization profiling (Biolog) and 16S rDNA, hsp60 and rpoB sequence analysis were done. Enterobacter cloacae was identified as the causal agent of this outbreak on chili pepper seedlings.

• 드라이브 ㆍ 컨트롤
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• 제13권4호
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• pp.23-30
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• 2016

This paper presents a control-oriented dynamical model of a towing rope system with variable-length. In this system, a winch driven by a motor's torque uses the towing rope to pull a cart. In general, it is a difficult and complicated process to obtain an accurate mathematical model for this system. In particular, if the rope length is varied by operating the winch, the varying rope dynamics needs to be considered, and the key physical parameters need to be re-identified... However, real time parameter identification requires long computation time for the control scheme, and hence undesirable control performance. Therefore, in this article, the rope is modeled as a straight massless segment, with the mass of rope being considered partly with that of the cart, and partly as halfway to the winch. In addition, the changing spring constant and damping constant of the towing rope are accounted for as part of the dynamics of the winch. Finally, a reduced-order observer-based servomechanism controller is designed for the system, and the performance is evaluated by computer simulation.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.81.1-81
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• 2003

Although plants have evolved to possess various defense mechanisms from local biotic and abiotic stressors, most of yield loss is caused by theses stressors. Recent studies have revealed that several different stress responsive reactions are inter-networking. Therefore, the identification and dissection of stress responsive genes is an essential and first step towards understanding of the global defense mechanism in response to various stressors. For this purpose, we applied cDNA microarray analysis, because it has powerful ability to monitor the global gene expression in a specific situation. To date, more than 10,000 non-redundant genes were identified from seven different cDNA libraries and deposited in our EST database (http://plant.pdrs.re.kr/ks200201/pepper.html). For this study, we have built 5K cDNA microarray containing 4,685 unigene clones from three different cDNA libraries. Monitoring of gene expression profiles of hot pepper interactions with biotic stress, abiotic stresses and chemical treatments will be presented. Although this work shows expression profiling at the sub-genomic level, this could be a good starting point to understand the complexity of global defense mechanism in hot pepper.

• Parasites, Hosts and Diseases
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• 제26권2호
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• pp.127-132
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• 1988

이형흡충류의 하나인 Stictodora속 흡충 2마리가 구충제 및 하제를 투여한 24세 남자환자의 설 사변에서 검출되었다. 총체는 길이 0.90∼0.98 mm, 폭 0.38 mm로 소형이며 특이한 복흡반-생식만(ventrogenital) 장치가 있고 생식공과 별개인 gonotyl(12∼15개의 미소한 갈고리 보유)을 가지고 있었다. 충체는 형태학적으로 Stictodora fuscatum(Onji and Nishio, 1916)과 매우 비슷하였으나 다수의 충체를 획득할 때까지 확정적인 종 동정은 보류하였다. 환자는 숭어, 농어 등 반염수산 어류를 즐겨 생식한다고 하였다. 이들이 Stirtodora sp.는 물론 따로 보고한 3종류 이형홉충의 감염원이었을 것으로 추측된다. 이 중례는 문헌상 Stictodora속 홉충의 최초 인체기생례에 해당된다.

• BMB Reports
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• 제48권2호
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• pp.121-126
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• 2015

Here we report a new chemical inhibitor against HIV-1 with a novel structure and mode of action. The inhibitor, designated as A1836, inhibited HIV-1 replication and virus production with a 50% inhibitory concentration ($IC_{50}$) of $2.0{\mu}M$ in an MT-4 cell-based and cytopathic protection antiviral assay, while its 50% cytotoxic concentration ($CC_{50}$) was much higher than $50{\mu}M$. Examination of the effect of A1836 on in vitro HIV-1 reverse transcriptase (RT) and integrase showed that neither were molecular targets of A1836. The characterization and re-infection assay of the HIV-1 virions generated in the presence of A1836 showed that the synthesis of early RT products in the cells infected with the virions was inhibited dose-dependently, due in part to abnormal protein formation within the virions, thus resulting in an impaired infectivity. These results suggest that A1836 might be a novel candidate for the development of a new type of HIV-1 inhibitor.