• Title/Summary/Keyword: Raw corn meal saccharifying enzyme

Search Result 2, Processing Time 0.018 seconds

Screening and Identification of a Potent Fungus for Producing Raw Corn Meal Saccharifying Enzyme (옥수수 생 전분 당화 효소 생산 곰팡이의 분리 및 동정)

  • 오성훈;오평수
    • Microbiology and Biotechnology Letters
    • /
    • v.18 no.6
    • /
    • pp.547-552
    • /
    • 1990
  • We have been searching microorganisms which produce highly active raw starch saccharifying enzyme and also have a good cultivation characters in submerged culture. About 170 strains of molds isolated from soil and compost were tested for their amylase productivity on plate contained 2% raw corn meal. Thirty-four strains out of 170 strains produced clearance on the plates, and were tested for their raw starch saccharifying activity. Then, 4 strains which had shown relatively high levels of saccharifying activity were selected. Among them, Strain No. 55 was found to have highest level of raw starch saccharifying activity, and selected for the further studies. In this paper, the morphological, physiological and cultural characteristics of Strain No. 55 were described. Based on the results obtained in these experiments, Strain No. 55 was identified to be a similar species to Aspergillus niger.

  • PDF

Improvement of Aspergillus niger 55, a Raw Corn Meal Saccharifying Enzyme Hyperproducer, through Mutation and Selective Screening Techniques (옥수수 生 전분 당화 효소 高 생산성 변이주 개발)

  • Oh, Sung-Hoon;O, Pyong-Su
    • Microbiology and Biotechnology Letters
    • /
    • v.19 no.2
    • /
    • pp.140-146
    • /
    • 1991
  • Mutation experiments were performed to select the mutant of Aspergillus niger 55, which had lost almost all the ability to produce transglucosidases but retained that of high productivity of raw meal saccharifying enzyme, by means of successive induction with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), ultraviolet(UV) light, and ${\gamma}$-rays. Also, we used the mutant enrichment techniques, such as liquid culture-filtration procedure and differential heat sensitivity of conidia, in order to increase the possibility of obtaining a mutant. The glucoamylase productivity of mutant PFST-38 was 11 times higher than that of the parent strain. The mutant PFST-38 was morphologically identical to the parent strain, except for the size of conidia, the tendency to form conidia and the lenght of conidiophore. Asp. niger mutant PFST-38 apeared to be useful for the submerged production of the raw corn meal saccharifying enzyme.

  • PDF