• Asian-Australasian Journal of Animal Sciences
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• v.17 no.2
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• pp.164-167
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• 2004

This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing $500{\mu}l$ mTCM199 maturation medium. The sperm rich portion of ejaculates was collected into a 250 ml insulated vacuum bottle and gradually cooled 22 to $24^{\circ}C$ over a 2 h period. Semen was centrifuged at 800 g for 10 min and the seminal plasma discarded. Sperm were esuspended in a lactose-egg yolk and N-acetyl-Dglucosamine (LEN) diluent to contain $1{\times}10^{9}$ sperm/ml and cooled to $5^{\circ}C$ over a 2 h period. Immediately before freezing, semen was rediluted with an equal volume of LEN+4% glycerol and packed into 5 ml straws. After thawing of the 5 ml straw, the 5 ml semen was diluted with 20 ml Beltsville thawing solution (BTS) at room temperature. Oocytes were inseminated with untreated (unwashed and nonpreincubated) or treated sperm (washed two times in BTS, mTLP-PVA and mTBM media, respectively and nonpreincubated) with $2{\times}10^{7}$ sperm concentration. Oocytes were coincubated for 6 h in $500{\mu}l$ mTBM fertilization. At 6 h after IVF, oocytes were transferred into $500{\mu}l$ NCSU-23 culture medium for further culture of 6 h. Sperm penetration, polyspermy and male pronuclear formation of oocytes at 12 h after IVF and developmental ability of oocytes at 48 h after IVF were evaluated. Sperm penetration rate, male pronuclear formation and rate of cleaved embryos were higher in the BTS, mTLP-PVA and mTBM treatments than the unwashed treatment (p<0.05). The rate of blastocysts from the cleaved oocytes (2-4 cell stage) were higher in the mTLP-PVA treatment than in the unwashed, BTS and mTBM treatments. In conclusion, we recommend the washing of frozen-thawed sperm with mTLP-PVA medium before in vitro fertilization of oocytes in mTBM medium.

• Journal of the Korea institute for structural maintenance and inspection
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• v.18 no.6
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• pp.90-96
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• 2014

The electrical resistivity of concrete can be related to two processes involved in corrosion of reinforcement: initiation (chloride penetration) and propagation (corrosion rate). The resisistivity of concrete structure exposed to chloride indicates the risk of early corrosion damage, because a low resistivity is related to rapid chloride penetration and to high corrosion rate. Concrete resistivity is a geometry-independent material property that describes the electrical resistance, which is the ratio between applied voltage and resulting current in a unit cell. In previous study, it was realized that the resistivity of concrete depended on the moisture content in the concrete, microstructural properties, and environmental attack such as carbonation. The current is carried by ions dissolved in the pore liquid. While some data exist on the relationship between moisture content on electrical resistivity of concrete, very little research has been conducted to evaluate the effect of chloride on the conduction of electricity through concrete. The purpose of this study is to examine and quantify the effect of chloride content on surface electrical resistivity measurement of concrete. It was obvious that chloride content had influenced the resistivity of concrete and the relationship showed a linear function. That is, concrete with chloride ions had a comparatively lower resistivity. Decreasing rate of resistivity of concrete was clear at early time, however, after 50 days resistivity was constant irrespective of chloride concentration. Conclusively, this paper suggested the quantitive solution to depict the electrical resistivity of concrete with chloride content.

• Journal of Korean Society of Transportation
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• v.36 no.1
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• pp.38-50
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• 2018

Emerging automated driving environments will lead to a mixed traffic flow depending on the interaction between automated vehicles (AVs) and manually driven vehicles (MVs) because the market penetration rate (MPR) of AVs will gradually increase over time. Understanding the characteristics of mixed traffic conditions, and developing a method to control both AV and MV maneuverings smoothly is a backbone of the traffic management in the era of automated driving. To facilitate smooth vehicle interactions, the maneuvering of AVs should be properly determined by various traffic and road conditions, which motivates this study. This study investigated whether the aggressiveness of AV maneuvering, defined as automated driving aggressiveness (ADA), affect the performance of mixed traffic flow. VISSIM microscopic simulation experiments were conducted to derive proper ADAs for satisfying both the traffic safety and the operational efficiency. Traffic conflict rates and average travel speeds were used as indicators for the performance of safety and operations. While conducting simulations, level of service(LOS) and market penetration rate(MPR) of AVs were also taken into considerations. Results implies that an effective guideline to manage the ADA under various traffic and road conditions needs to be developed from the perspective of traffic operations to optimize traffic performances.

• Journal of the Korea Concrete Institute
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• v.27 no.1
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• pp.85-92
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• 2015

The chloride ions, responsible for the initiation of the corrosion mechanism, intrude from the external medium into the concrete. A part of the intruding chloride ions will be retained by the hydration products of the binder in concrete, either through chemical adsorption or by physical adsorption. Since the hydration products of cement are responsible for the chloride binding in concrete, this study focused on the chloride binding in individual hydrate. The purpose of this study is to explore the time dependant behaviors of chloride ions adsorption with cement hydrates, focused on its mechanism. AFt phase and CH phase were not able to absorb chloride ion, however, C-S-H phase and AFm phase had a significant chloride adsorption capacity. In particular, AFm phase showed a chemical adsorption with slow rate in 40 days, while C-S-H phase showed binding behaviors with 3 stages including momentary physical adsorption, physico-chemical adsorption, and chemical adsorption. Based on the results, this study suggested theoretical approach to depict chloride adsorption behavior with elapsed time of C-S-H phase and AFm phase effectively. It is believed that the approach suggested in this study can provide us with a good solution to understand the mechanism on chloride adsorption with hydrates and to calculate a rate of chloride penetration with original source of chloride ions, for example, marine sand at initial time or sea water penetration later on.

• Fire Science and Engineering
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• v.29 no.4
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• pp.67-72
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• 2015

A series of fire tests was conducted to examine the fire characteristics of flaming and smoldering combustion of engineered wood products, which have been widely used for furniture and finishing materials in buildings. The engineered wood products of MDF, plywood, and chipboard were ignited by a radiant cone heater with incident heat flux of $50kW/m^2$. During the fire test, key parameters representing the fire characteristics such as the heat release rate, yield rate of combustion product, and effective heat of combustion were quantified in terms of thickness. The tests show two peak points of HRRPUA due to lateral fire propagation in the initial stage, followed by later fire penetration through the specimen thickness. The mass loss rate of flaming combustion was 5 times higher than that of smoldering combustion, while the CO yield rate of smoldering combustion was 10 times higher than that of flaming combustion. This study can contribute to the understanding of fire behavior of wood combustibles and provide useful data for fire analysis.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.1
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• pp.61-69
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• 2015

The objective of this study is to experimentally investigate the mixed flow behaviors and oxygen transfer characteristics of a vertical orifice ejector. The experimental apparatus consisted of an electric motor-pump, an orifice ejector, a circulation water tank, an air compressor, a high speed camera unit and control or measurement accessories. The mass ratio was calculated using the measured primary flow rate and suction air flow rate with experimental parameters. The visualization images of vertically injected mixed jet issuing from the orifice ejector were qualitatively analyzed. The volumetric oxygen transfer coefficient was calculated using the measured dissolved oxygen concentration. At a constant primary flow rate, the mass ratio and oxygen transfer coefficient increase with the air pressure of compressor. At a constant air pressure of the compressor, the mass ratio decreases and the oxygen transfer coefficient increases as the primary flow rate increases. The residence time and dispersion of fine air bubbles and the penetration of mixed flow were found to be important parameters for the oxygen transfer rate owing to the contact area and time of two phases.

• Fire Science and Engineering
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• v.34 no.3
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• pp.28-34
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• 2020

In this study, an ISO 5660-1 cone calorimeter experiment was conducted to examine the effects of changes in flow and thermal thickness around solid combustibles on heat release rate characteristics. Polymethyl methacrylate (PMMA) is a solid combustible material that does not generate char during the combustion reaction. Hence, it was selected for the experiment, and the thermal penetration depth was calculated to distinguish the thermal thickness of PMMA. Furthermore, the thermal decomposition characteristics were analyzed by measuring the heat release rate measured during the combustion of PMMA. This was performed after generating the forced flow around the combustibles by setting the duct flow of the cone calorimeter to 12, 24, and 40 L/s. The results confirmed that the thermal release rate of the thermally thin combustible material was not significantly affected by the change in the surrounding flow. Hence, the thermally thick combustible material was significantly affected by the change in the flow rate.

• Korean Journal of Animal Reproduction
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• v.27 no.2
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• pp.153-161
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• 2003

This study has evaluated effect of the spermatozoa incubation on the glycosidase activity and fertilizing ability in vitro in the pig. To identify sperm glycosidases specific for sugar residues found in the zona pellucida of pig oocytes, the spermatozoa were treated experimentally and assayed for activities of $\alpha$-L-fucosidase, $\alpha$-D-mannosidase, $\beta$-D-galactosidase and N-acetyl-$\beta$-D-glucosaminidase ($\beta$-GlcNAc'ase). The glycosidases activity were higher in spermatozoa incubated for 2h than without incubation. The $\beta$-GlcNAc'ase activity was at least two-fold higher than other glycosidase regardless of spermatozoa incubation. In the same glycosidases, the activity had a tendency to increase as time of spermatozoa incubation was prolonged, but there were no differences in spermatozoa incubated during the various periods (4~24h). The percentages of spermatozoa that reached acrosome reaction were affected by glycosidases in the medium (P<0.05, for mannosidase), and were higher in spermatozoa with that than without incubation. On the other hand, the spermatozoa motility were decreased with incubation periods, but no effects by different glycosidases on the change of sperm motility during the various periods of incubation. In other experiment, the binding and penetration of pig spermatozoa were tested with oocytes matured in vitro in the presence of various glycosidase. The penetration rates were decreased with incubation of spermatozoa when oocytes were inseminated in medium with different glycosidases. These rates were higher in spermatozoa non-incubated than with incubation for 2h (P<0.05 for GlcNAc'ase; P<0.01 for control group). The sperm-zona binding rate in control group were higherthan in medium with glycosidases. In addition, the highest binding rate were obtained in medium with GlcNAc'ase. In all glycosidases, the sperm-zona binding rate in spermatozoa without incubation were higher than incubation for 2h. The significant differences were obtained in spermatozoa treated with $\alpha$-D-mannosidase (P<0.05). These results suggest that $\beta$-GlcNAc'ase is present mainly in the plasma membrane of pig spermatozoa. It was also shown that the glycosidase activity were increased in all glycosidases in spite of low sperm-zona binding rate and penetration rates by spermatozoa incubation.

• Progress in Medical Physics
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• v.27 no.4
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• pp.236-240
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• 2016

The purpose of this study is to propose a novel high sensitivity neuro-PET design. The improvement of sensitivity in neuro-PET is important because it can reduce scan time and/or radiation dose. In this study, we proposed a novel PET detector design that combined conical shape detector with cylindrical one to obtain high sensitivity. The sensitivity as a function of the oblique angle and the ratio of the conical to cylindrical portion was estimated to optimize the design of brain PET using Monte Carlo simulation tool, GATE. An axial sensitivity and misplacement rate by penetration of ${\gamma}$ rays were also estimated to evaluate the performance of the proposed PET. The sensitivity was improved by 36% at the center of axial FOV. This value was similar to the calculated value. The misplacement rate of conical shaped PET was about 5% higher than the conventional PET. The results of this study demonstrated the conical detector proposed in this study could provide subsequent improvement in sensitivity which could allow to design high sensitivity PET for brain imaging.

• Journal of Hydrogen and New Energy
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• v.10 no.4
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• pp.197-210
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• 1999

The hydrogen storage performance and electrochemical properties of $Zr_{1-X}Ti_X(Mn_{0.2}V_{0.2}Ni_{0.6})_{1.8}$(X=0.0, 0.2, 0.4, 0.6) alloys are investigated. The relationship between discharge performance and alloy characteristics such as P-C-T characteristics and crystallographic parameters is also discussed. All of these alloys are found to have mainly a C14-type Laves phase structure by X-ray diffraction analysis. As the mole fraction of Ti in the alloy increases, the reversible hydrogen storage capacity decreases while the equilibrium hydrogen pressure of alloy increases. Furthermore, the discharge capacity shows a maxima behavior and the rate-capability is increased, but the cycling durability is rapidly degraded with increasing Ti content in the alloy. In order to analyze the above phenomena, the phase distribution, surface composition, and dissolution amount of alloy constituting elements are examined by S.E.M., A.E.S. and I.C.P. respectively. The decrease of secondary phase amount with increasing Ti content in the alloy explains that the micro-galvanic corrosion by multiphase formation is little related with the degradation of the alloys. The analysis of surface composition shows that the rapid degradation of Ti-substituted Zr base alloy electrode is due to the growth of oxygen penetration layer. After comparing the radii of atoms and ions in the electrolyte, it is clear that the electrode surface becomes more porous, and that is the source of growth of oxygen penetration layer while accelerating the dissolution of alloy constituting elements with increasing Ti content. Consequently, the rapid degradation (fast growth of the oxygen-penetrated layer) with increasing Ti substitution in Zr-based alloy is ascribed to the formation of porous surface oxide through which the oxygen atom and hydroxyl ion with relatively large radius can easily transport into the electrode surface.