• 제목/요약/키워드: Rat submandibular gland

검색결과 39건 처리시간 0.033초

백서의 DMBA 유도 악하선종양에서의 $Na^+,K^+$-ATPase에 대한 연구 (A STUDY OF $Na^+,K^+$-ATPase IN THE RAT SUBMANDIBULAR GLAND TUMOR INDUCED BY DMBA)

  • 이의웅
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제13권1호
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    • pp.1-8
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    • 1991
  • 체중 100g 내외의 Sprague Dawley계 정상백서 암, 수 각 50마리씩을 실험동물로 하여 분말형태의 순수 DMBA를 소구(pellet)로 압착제작하여 우측 악하선에 매식후 즉시, 1,3,5,7,9,11,13,15,17주째 10마리씩 희생시켜 좌, 우측악하선을 적출, 무게를 측정하고, 육안적, 조직학적, 생화학적으로 실험기간에 따라 비교분석하여 다음과 같은 결론을 얻었다. 1. 매식후 11 주째부터 우측 경부 악하선부위의 종창을 관찰할 수 있었으며, 결절덩어리를 촉진할 수 있었다. 2. 우측악하선의 무게의 크기는 실험기간의 경과에 따라 괄목할만한 증가를 보였다(매식후 17주째는 좌측에 비해 약 5.5배의 무게를 나타냈다). 3. 매식후 7주와 11주째부터 조직학적으로 상피이형성과 침윤성 유표피암의 소견을 각각 관찰할 수 있었다. 4. 우측 악하선에서의 유표피암의 발생율은 매식후 17주째 76%이었다. 5. DMBA 는 현저히 억제된 $Na^+,K^+$-ATPase 활성도를 야기하며 실험기간의 경과의 따라 활성도 비율도 현저히 저하시킨다.

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Streptozotocin유도 당뇨병이 백서 악하선에 미치는 영향에 관한 병리조직학적 연구 (Effect of the Streptozotocin Induced Diabetes in the Rat Submandibular Glands)

  • Hung-Mo Kim;Jung-Pyo Hong
    • Journal of Oral Medicine and Pain
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    • 제19권1호
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    • pp.45-55
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    • 1994
  • The purpose of this study was to observe the microscopic change of salivary gland tissues, which is the cause of xerostomia in diabetic condition: for this target the author injected STZ 0.1ml/100gm b.w. on rat to produce diabetes, and than observed microscopic change in submandibular gland through the histopathologic method, obtaining as follows : 1. All of the experimental specimens suffered diabetes after injection of STZ, but the blood glucose level was irregular. 2. There were not interrelationship between the blood glucose level and microscopic change on salivary gland tissues. 3. The salivary gland changed after diabetes initiation in lapse of times; after 14 days,suffered severe destruction, however after 17 days, it was regenerated. 4. Salivary glands showed congested, destructive acini cells, and hyperplastic ductal cells as well as salivary gland duct-like structures. 5. Then were accumulation of fat granules within the cytoplasm of the acini cells on mucous gland in diabetic condition. 6. According to insulin injection, there were no more changes on salivary gland tissues, even in the accumulation of fat granules. 7. Histological changes of the serous gland were obvious more than the mucous gland in this experimental condition.

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Distribution of Taste Receptors in Submandibular and von Ebner Salivary Glands

  • Jun, Yong-Ku;Kim, Se-Nyun;Lee, Cil-Han;Cho, Young-Kyung;Chung, Ki-Myung;Roper, Stephen D.;Kim, Kyung-Nyun
    • International Journal of Oral Biology
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    • 제33권1호
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    • pp.13-23
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    • 2008
  • Taste is a critically important sense for the survival of an organism. However, structure and distribution of taste receptors were only recently investigated. Although expression of the ion channels responsible for the sense of salty taste and acidity was observed in the non-taste cells, receptors for sweet and bitter taste were only identified in taste cells. Salivary glands are involved in the sensing of taste and plays important roles in the transduction of taste. The purpose of this study is to examine whether taste receptors are present in the salivary glands and to provide clues for the investigation of the taste-salivary glands interaction. Using microarray and RT-PCR analyses, the presence of taste receptor mRNAs in the rat von Ebner gland and submandibular gland was confirmed. Type I taste receptors were preferentially expressed in von Ebner gland, whereas type II taste receptors were expressed in both von Ebner gland and submandibular gland. The tastespecific signal tranducing proteins, $G_{\alpha}gustducin$ and phospholipase C ${\beta}2$, were also detected in both salivary glands by immunohistochemistry. Finally, the activation of the calcium signal in response to bitter taste in the acinar cells was also observed. Taken together, these results suggest that taste receptors are present in the von Ebner gland and submandibular gland and that type II taste receptors are functionally active in both salivary glands.

방사선 조사가 백서 악하선조직에 미치는 영향에 관한 실험적 연구 (AN EXPERIMENTAL STUDY OF THE IRRADIATION EFFECTS ON RAT SUBMANDIBULAR GLAND)

  • 이창환;이상래
    • 치과방사선
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    • 제20권1호
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    • pp.139-148
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    • 1990
  • The purpose of this study was to investigate the effects of irradiation on the rat submandibular gland which composed of the epithelial growth factors. The experimental animals were the Sprague Dawley strain rats, which were the rats as the non-irradiated control group and the rats as the experimental group which were divided into groups as the experimental duration of 1 hour, 3 hours, 6 hours, 12 hours, 1 day, 3 days, 1 week, 2 weeks, 4 weeks. The experimental animals were singly irradiated at a dose of 8 Gray gamma ray to their head and neck region by the Co-60 teletherapy unit and sacrificed after each experimental duration. The specimens were stained with H-E and Azan stain and examined light microscopically. The results of this study were obtained as follows. 1. The all of mucous and serous acini in submandibular gland showed similar pattern of changes in structure according to the lapse of time. 2. The acinic cells started to change after 1 hour, and repaired after 12hours with mitosis and proliferation of the cells between acini. The changes were marked after 1day, and repaired gradually in course of time. 3. The duct were dilatated irregularly, and the outline of the eosinophilic stained ductal cells changed indistinctly.

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Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats

  • Jeong, Moon-Jin;Lee, Myoung-Hwa;Lim, Do-Seon;Jeong, Myeongju;Jeong, Soon-Jeong
    • 치위생과학회지
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    • 제22권2호
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    • pp.83-89
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    • 2022
  • Background: Estrogen deficiency affects the structure and function of the salivary glands in women, leading to a decrease in salivary secretion and a change in the composition of saliva. Previous studies on changes in the salivary glands that cause estrogen deficiency have reported only partial results for the parotid and submandibular glands, and there are few comparative morphological studies of histological changes between the parotid and submandibular glands in ovariectomized rats (OVX) leading to estrogen deficiency. This study aimed to analyze the histopathological and histochemical changes in the parotid and submandibular salivary glands causing estrogen deficiency by using OVX, and to discuss the mechanism on these changes. Methods: The parotid and submandibular glands from sacrificed control and OVX groups were fixed with cold 4% paraformaldehyde in phosphate buffer (pH 7.2). The tissues were dehydrated using a series of graded ethyl alcohol and embedded in paraffin. For histopathological analysis, sections cut to a thickness of 6 to 7 ㎛ were stained with hematoxylin and eosin (H&E). For histochemical analysis, Periodic acid-Schiff (PAS), Alcian blue (AB, pH 2.5), and PAS+AB (pH 2.5 and pH 1) staining was performed. Results: Histopathological analysis of OVX tissue showed that the parotid and submandibular salivary glands were broadly and clearly separated and divided into lobes. In OVX, acinar and ductal cells with condensed polymorphic or pyknotic nucleus, which are presumed to be characteristic of apoptotic cells, and degenerated cells with lipid deposition in cytoplasmic granules and ruptured membranes were increased. Histochemical analysis of OVX, confirmed an increase in the number and acidification of acinar secretory granules. Conclusion: Histopathological and histochemical changes and the effects of estrogen deficiency are more evident in the submandibular salivary gland than in the parotid gland.

Extracellular Matrix Metalloproteinase Inducer is Regulated Developmentally and Functionally in the Rat Submandibular Gland

  • Yoo, Hong-Il;Suh, Han-Young;Kim, Sun-Hun
    • International Journal of Oral Biology
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    • 제40권3호
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    • pp.127-133
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    • 2015
  • The salivary gland undergoes complex process of growth and differentiation of the branching morphogenesis of ductal system during the prenatal and early postnatal periods which are regulated by various elements in the extracellular matrix. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a cell adhesion molecule. In the present study, localization and expression of EMMPRIN in development and effects of chorda-lingual denervation and cyclosporine A (CsA) treatment on the EMMPRIN expression were investigated. Immunohistochemistry, RT-PCR and Western blot were used to determine expression level. Immunohistochemistry revealed that EMMPRIN was localized specifically in the cytoplasm of ductal cells, not acini of the submandibular gland all the postnatal periods. At prenatal day 18, when the formation of ducts was not definite, no immunoreactivity was observed. Both Western blot and RT-PCR analyses revealed that EMMPRIN expression was maintained up to postnatal day 7, decreased after postnatal day 10. The EMMPRIN expression was upregulated by the surgical denervation of the chorda-lingual nerve in the gland as well as by the CsA treatment. The present study suggests that EMMPRIN is a crucial molecule for maintaining physiological functions of the salivary gland.

악하선 분비관의 결찰 후 타액선 조직의 변화 (THE CHANGES OF SALIVARY GLAND AFTER THE LIGATION OF THE EXCRETORY DUCT IN SUBMANDIBULAR GLANDS)

  • 차승만;정인교
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제31권5호
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    • pp.379-389
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    • 2005
  • Obstructive adenitis of the salivary gland following salivary stone or infectious disease of the gland and surrounding tissues is a common disease. It is often difficult to decide whether to perform total excision of the gland or to consider conservative treatment. The present study was designed to investigate histological, histochemical changes of submandibular gland after ligating the excretory duct for identifying the results of gland duct obstruction. A group of 40 rat of Sprague-Dawley weighing about $200{\sim}220gm$ were used in the present study. 30 rats had ligation of the main excretory ducts of submandibular glands just at the exit from the glands. For controls, 10 rats had a sham operation without duct ligation. They were inducted into euthanagia state by intracardial Ketamine injection in 1, 2, 3, 5, 7, 14, 21, 28, 35 and 42 days after the ligation. In each ligation period, 3 animals were used for ligation and one animal was for control. The submandibular glands were dissected out at sacrifice and stained with H&E, PAS, mucicarmine stain and histological examination were carried out under the light microscope. After examination and comparison of all specimens, the results were as follows: 1. In the features of H&E stain, acini disappeared by degrees after the ligation of the excretary duct and interstitial cells were displaced into fibrous connective tissue. Salivary gland had been atrophied with enlarging ducts and proliferating ductal cells. 2. Through total experimental period, a lot of vessels were observed and the atrophy of serous gland was severer than that of mucous gland. 3. The deep portion of submandibular glands showed severe degeneration rather than superficial portion of them after the ligation. 4. The changes which had enlarged ducts and proliferating ductal cells were observed in entire gland and more prominent in serous gland than mucous gland after the ligation. 5. Although PAS and mucicarmine reactions were decreased gradually after the ligation with the lapse of time, since 2 to 3 weeks they were strong positive reactions on entire gland, especially on duct-like structure. So, we can suggest that salivary gland will be atrophied but, survived acini will be redistributed around the ducts.

Sympathetic and parasympathetic regulation of sodium transporters and water channels in rat submandibular gland

  • Jung, Hyun;Ryu, Sun-Youl
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제32권1호
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    • pp.1-7
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    • 2006
  • The present study was aimed to explore the role of sympathetic and parasympathetic nerves in the regulation of sodium transporters and water channels in the salivary gland. Rats were denervated of their sympathetic and parasympathetic nerves to the submandibular gland, and the glandular expression of sodium transporters and water channels was determined by Western blot analysis. The expression of either ${\alpha}1$ or ${\beta}1$ subunit of Na, K-ATPase was not significantly affected either by the sympathetic or by the parasympathetic denervation. The expression of subunits of epithelial sodium channels was significantly increased both in the denervated and contralateral glands either by the sympathetic or by the parasympathetic denervation. Neither the sympathetic nor the parasympathetic denervation significantly altered the expression of aquaporin-1 (AQP1). Nor was the expression of AQP4 affected significantly by the parasympathetic or the sympathetic denervation. On the contrary, the expression of AQP5 was significantly increased not only by the parasympathetic but also by the sympathetic denervation. These results suggest that sympathetic and parasympathetic nerves have tonic regulatory effects on the regulation of certain sodium transporters and AQP water channels in the salivary gland.

Autonomic Neural Regulation of Sodium Transporters and Water Channels in Rat Submandibular Gland

  • Ryu, Sun-Yeol;Jung, Hyun;Kim, Ki-Yung;Kim, Mi-Won;Lee, Jong-Un
    • The Korean Journal of Physiology and Pharmacology
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    • 제10권2호
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    • pp.65-69
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    • 2006
  • The present study was undertaken to explore the role of autonomic nerves in the regulation of sodium transporters and water channels in the salivary gland. Rats were denervated of their sympathetic or parasympathetic nerves to the submandibular gland. One week later, the expression of Na,K-ATPase, epithelial sodium channels (ENaC), and aquaporins (AQP) was examined in the denervated and contralateral glands. The sympathetic denervation slightly but significantly decreased the expression of ${\alpha}1$ subunit of Na,K-ATPase, whereas the parasympathetic denervation increased it. The expression of ${\alpha}$-subunit of ENaC was significantly increased in both the denervated and contralateral glands either by the sympathetic or parasympathetic denervation. The sympathetic denervation significantly increased the expression of AQP5 in both the denervated and contralateral glands, whereas the parasympathetic denervation decreased it. It is suggested that the autonomic nerves have a tonic effect on the regulation of sodium transporters and AQP water channels in the salivary gland.

방사선조사가 악하선세포내 분비과립의 미세구조에 미치는 영향에 관한 실험적 연구 (AN EXPERIMENTAL STUDY OF THE RADIATION-INDUCED CHANGES ON THE SECRETORY GRANULES OF THE RAT SUBMANDIVULAR GLAND ACINAR CELL)

  • 최미;이강숙;최갑식
    • 치과방사선
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    • 제24권2호
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    • pp.403-410
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    • 1994
  • The purpose of this study was to investigate the irradiation effects on the secretory granules of the rat submandibular gland acinar cell. For this study, Sprague-Dawley strain rats were irradiated to their neck region with the dose of 5Gy and l0Gy by 6MV X-radiation, and sacrificed on the experimental periods after irradiation. The authors observed the ultrastructural changes of the secretory granules of the rat submandibular gland acinar cell under a trasmission electron microscope. The results were as follows: In the transmission electron microscopic examination, secretory granules were blurred in limiting membrane on the 3hours groups after irradiation. And they showed decrease in number, irregularities in shape and distributional pattern, and inhomogeneous internal electron density on the Iday and 3days groups. After then, these changes were recovered in shape and distributional pattern on the 14days groups, and changes of internal electron density and limiting membrane were recovered on the 28days groups after irradiation. Among the intracellular organelles, rough endoplasmic reticulum was scattered, but golgi complex was not changed. And such pathologic changes were earlier and more prominent in 10Gy irradiated groups than in 5Gy irradiated groups.

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