• 대한수의학회지
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• 제46권4호
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• pp.371-379
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• 2006

Newcastle disease virus (NDV) is a single-stranded negative sense RNA virus, which has been classified as a member of the Avulavirus genus of the Paramyxoviridae family. It is also one of the most important pathogens in the poultry industry. The glycoproteins, fusion (F) and hemagglutinin-neuraminidase (HN), determine the virulence of NDV, and the relevant molecular structures have already been determined. NDV isolates differ in terms of virulence, and at least 2 of 9 genotypes (I-IX) have been shown to co-circulate. Therefore, it is clearly important to differentiate between vaccine strains and field isolates. In vivo pathogenicity tests have been the standard protocol for some time, but molecular methods appear preferable in terms of the rapidity of diagnosis, as well as animal welfare concerns. In this study, we have designed primer sets from HN gene for phylogenetic analysis and restriction enzyme analysis, and from F gene for pathotype-specific RT-PCR. Via the combination of 2 methods, 106 Korean NDV isolates obtained from 1980 to 2005 were differentiated into vaccine strains, and virulent genotypes VI and VII. The genotype VI viruses were only rarely isolated after 1999, and genotype VII, after it was initially isolated from poultry in 1995, recurred in 2000, and then became the main NDV constituting a threat to the Korean poultry industry.

• 대한유전성대사질환학회지
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• 제15권3호
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• pp.138-146
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• 2015

Purpose: If early diagnosis is not made, patients with metabolic disorders as homocystinemia rapidly progress to physical defect or mental retardation resulted in storage of the toxic material into the brain. Therefore, it is necessary to develop an analytical method for a rapid screening and/or correct confirmation diagnosis. Methods: The standard solution of sulfur amino acids spiked plasma was subjected to protein precipitation with methanol, and then consecutively derivatized with trimethylsilyl (TMS) and trifluoroacyl (TFA) and determined by GC-MS. The formation of TMS derivative of the hydroxyl and TFA derivative of amino functional group was performed by BSTFA and MBTFA, respectively. Selective ion monitoring (SIM) mode was used for quantification with selected specific ions. Results: A calibration curve on standard spiked pooled plasma showed a linear relationship with correlation coefficient of 0.9936-0.9992 for all compounds over the range of 0.1-300 ng. The precision and accuracy were within S.D. of 1-15% and RSD of 1-15% for intra-day assay at 2 ng/mL, 15 ng/mL and 30 ng/mL. LOD and LOQ was 0.4 ng/mL and 4 ng/mL respectively. Conclusion: A rapid analytical method was developed to quantify sulfur amino acids and methyl malonic acid, after two-step derivatization procedure with good sensitivity and specificity on human plasma. Advantages of a new method are simplicity and rapidity. The method could be useful for routine analysis, diagnosis of homocysteinemia.

• Childhood Kidney Diseases
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• 제14권2호
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• pp.218-222
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• 2010

최근의 유행한 신종 H1N1 S-OIV는 전세계적으로 퍼지고 있고, 이에 동반된 호흡기 질환등의 여러 합병증들이 보고되었다. 신증후군은 간염바이러스 등의 감염 후에 발현될 수 있는 것으로 알려져 있으나 S-OIV 감염 후에 발생한 예는 보고된 적이 없는 실정이다. 저자들은 S-OIV 감염으로 확진된 환아에서 발생한 신증후군이 발생하였으나 스테로이드 치료로 쉽게 치료된 경험을 하였기에 이를 보고하는 바이다.

• Journal of Pharmaceutical Investigation
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• 제24권2호
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• pp.85-94
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• 1994

Inclusion complexes of ketoconazole (KT) with ${\alpha}-$, ${\beta}-cyclodextrin$ (CD) and dimethyl-${\beta}-cyclodextrin$ $(DM{\beta}CD)$ in a molar ratio of 1:2 were prepared by freeze-drying and solvent evaporation methods. The interactions of KT with ${\alpha}-CD$, ${\beta}-CD$ and $DM{\beta}CD$ in aqueous solution and in solid state were investigated by solubility study, infrared (lR) spectroscopy and differential scanning calorimetry (DSC). The stability constant of $KT-DM{\beta}CD$ inclusion complex (lC) was found to be the largest among three inclusion complexes. Clear differences in IR spectra and DSC curves were observed between inclusion complexes and physical mixtures (PM) of KT-CDs. It was also shown by IR spectra and DSC curves that solvent evaporation method might be. superior to the freeze-drying method in preparing the inclusion complexes of KT-CDs. The dissolution rate of KT was markedly increased by inclusion complex formation with CDs in the buffer solution at pH 4.0 and pH 6.8. The mean dissolution time (MDT,min), which represents the rapidity of dissolution, was in the order of $KT-DM{\beta}CD$ IC (3.20) < $KT-{\beta}-CD$ IC (4.36) < $KT-{\alpha}-CD$ IC (6.99) < $KT-{\alpha}-CD$ PM (17.46)< $KT-{\beta}-CD$ PM (19.36) < $KT-{\beta}-CD$ PM (28.53). The dissolution rates of KT-CD ICsprepared by solvent evaporation method were faster than those of KT-CD ICs prepared by freeze-drying method.

• 한국치위생학회지
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• 제12권1호
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• pp.225-233
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• 2012

Objectives : This thesis aims to provide basic materials for exploring trends and operational strategy of the cyber training. To achieve study purpose, it analyzed the satisfaction of trainees in regard to the education of field training course of Cyber Oral Health Promotion Program, established and operated between 2007 and 2009. Methods : This study conducted questionnaire surveys after the completion of training to utilize the satisfaction level of 989 respondents among 1,310 employees of public institutions who completed the field training course of Cyber Oral Health Promotion Program. Results : Respondents showed satisfaction on the training course throughout three years in terms of satisfaction of instructional methods (screen characteristics, educational techniques, and so on) according to educational contents, the connection and realization of online education and service satisfaction, necessary for learning in association with educational guidance, offered to learners by a tutor and rapidity in questions and answers. A majority of respondents in all years answered that they participated in training program voluntarily for self-development and improvement in work ability, and they thought that the completion of course would be helpful to their current work and future work. In addition, cyber training program was primarily conducted in the working place by 72.5%, and the most difficult thing in cyber training was to combine work and learning by 60.6%. Conclusions : As shown in the results above, workers in public institutions were satisfied with cyber job training and it was evaluated that cyber job training would be helpful to performing their actual work. Therefore, it is needed to collect and evaluate more diverse requirements of trainees with regard to cyber job training, and the development and operation of job training program that reflects these results sufficiently is required.

• 한국항만경제학회지
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• 제31권3호
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• pp.107-120
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• 2015

기업들의 국제물류체계는 물류비용과 시간의 절감뿐만 아니라 물류의 부가가치 창출 기능을 극대화하기 위해 급속히 발전할 전망이다. 글로벌 공급체인 운영방식인 Ro-Ro 운송은 운송시간의 정확성, 비용절감, 신속성, 안전성 향상 등에서 경쟁력이 높아 이를 활용하기 위한 전략이 급증할 것으로 전망된다. 이에 본 연구에서는 한일간 교류 물동량 분석을 통해 Ro-Ro 부두 조성의 적정 수요와 규모를 추정해 보고자 하였다. 이를 위해 한일간 Ro-Ro선의 운항속도에 근거한 운항거리를 바탕으로 1권역과 2권역으로 구분하여 부산항과의 물동량(컨테이너와 일반화물)을 분석하였다. 분석된 물동량을 바탕으로 Ro-Ro선 이용 가능성이 높은 품목의 비중을 추정한 후 한일간 Ro-Ro 부두의 이용가능 물동량을 추정하였다. 추정된 물동량을 처리하기 위해서는 2만톤급 2개 선석과 장치장 10만$m^2$ 정도가 필요한 것으로 판단된다. 부산항에 Ro-Ro 전용 부두 조성을 통해 컨테이너 일변도의 항만정책을 탈피하고, 글로벌 기업들의 수요에도 대응할 수 있을 것이다.

• KSBB Journal
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• 제13권6호
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• pp.693-699
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• 1998

고정화한 P. phosphoreum의 biolummescence악 독생물질과의 상관관계를 이용한 수계의 독성물질 momtonng 가능성을 조사하였다. P. phosphoreum을 이용한 수계의 독성물질 momtonng을 위한 적정 세포 농도는 최대 biolummescence intensnty를 보인 $O.D_{660}$ 1.0~1.2였으며 이 농도외 세포를 2.5% NaCl 용액에 희석하여 5.0%의 alginateo에 고정화 했을 때 biolummescence 유지도까 가장 좋았다. 이러한 방법으로 monitoring이 가능한 수계의 pH는 6.0~8.0이었다. Free cell과 고정화 세포로 $CdCl_2.H_20, PbCl_2, phenol $그리고 mtrophenol에 대한 bioluminescence를 조사하였을 때 고정화 방법에 의해 독성물질에 대한 민감성0] 증가되었다. 고정화 세포외 경우 각 독성 물질에 따라 20분 이내에 0.01~1.50ppm이하까지 mornitoring이 가능하였다. 뿐만 아니라 speciflC blolurninescence reclucLion rate, biolummescence mtenslLy의 ratio 그리고 Gamma value로 분석함으로서 독성물질의 농도와 biolummescence의 변화를 직선의 관계로 나타낼 수 있어 독성 물질의 농도 예측이 가능하다.

• 한국임상수의학회지
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• 제14권2호
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• pp.177-184
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• 1997

Canine parvovirus(CPV) is a very highly contagious virus causing hemorrhagic enteritis and myocarditis mainly in young dogs. The diseases were first recognized in 1978, and then spread throughout the world by 1980. The main source of the infection seems to be the feces of infected dogs, at the same time feces are suitable materials for detection of virus in the enteric form exactly for the same reasons. Recently, a new technique of in vitro DNA amplification, Known as the polymerase chain reaction (PCR), has been widely applied to clinical viral diagnosis because of its sensitivity, specificity and rapidity. In this research, we attemped to set up the PCR for the detection of CPV in fecal samples and conformed the canine parvpviral enteritis by PCR. To increase the sensitivity and specificity of a PCR, the nested PCR (two-step PCR) was performed. We also surveyed the contamination status of CPV in the research using fecal specimen was highly sensitive and specific. Of the 100 fecal specimens suspected canine parvoviral enteritis, 45 fecal specimens were positive in HA test, 64 fecal specimens were positive in the first PCR, and 87 fecal specimens were positive in the second PCR. CPV contamination status of animal clinics and breeding centers was serious, wo hygienic management of environment in which dogs are reared is required. The nested PCR described here seems to be a rapid, sensitive and specific for the detection of canine parvovirus.

• 한국축산식품학회지
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• 제34권3호
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• pp.339-345
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• 2014

This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.

• 한국식품과학회지
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• 제28권6호
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• pp.1177-1179
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• 1996

식품포장재에 함유되어있는 톨루엔 및 잔류용제를 용출분석하는 방법으로 headspace gas chromatography (static headspace method)를 이용하였다. 5종(methanol, isopropyl alcohol, ethyl acetate, methyl ethyl ketone, toluene)의 표준용제에 대한 머무름시간과 상대표준편차를 검토해 본 결과, 이상적인 머무름시간의 상대표준편차라 할 수 있는 0.15% 이하로 양호하였으며 재현성도 우수하였다. 인쇄된 포장재를 분석한 결과. 주로 methanol, toluene, methyl ethyl ketone이 검출되었고, methanol은 불검출${\sim}0.939\;mg/m^2$, toluene은 불검출${\sim}1.403{\;}mg/m^2$, methyl ethyl ketone은 불검출${\sim}0.932\;mg/m^2$ 함유 되어있는 것으로 나타났으며, 총 잔류 용제는 불검출${\sim}2.433{\;}mg/m^2$ 함유되어 있는 것으로 나타났다.