• Korean Journal of Clinical Laboratory Science
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• v.39 no.3
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• pp.161-166
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• 2007

To develop a rapid and reliable single-tube-based PCR technique for detection simultaneously the quinolone resistance qnrA, qnrB and qnrS genes. After multiple alignment, primers were designed to detect known qnr variants. I was used for A total of 43 extented-spectrum ${\beta}$-lactamases (ESBLs) producing Escherichia coli and Klebsiella pneumoniae isolated from university hospital were tested for screening, as with qnr genes. In optimized conditions, all positive controls confirmed the specificity of the PCR primers. Out of 43 isolates, qnrA genes were detected 19 (44.2%), qnrB genes 5 (11.7%), qnrS genes 15 (34.9%) and 8 (18.6%) isolates were not detected. I report here a fast and reliable technique for rapid screening of qnr positive strains to be used for epidemiological surveys.

• Proceedings of the Korea Concrete Institute Conference
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• 2009.05a
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• pp.467-468
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• 2009

In this study, seismic capacity of R/C school buildings are investigated on the basis of the 2nd and 3rd level procedures of the Japanese Standard for Evaluation of Seismic Capacity of Existing R/C Buildings, push-over analysis and nonlinear dynamic analysis, respectively. In order to verify the applicability of the new method proposed in this study, relationships between evaluation results of the proposed methods shown in Part-2 and detailed evaluation methods mentioned above are compared.

• Proceedings of the Korea Concrete Institute Conference
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• 2009.05a
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• pp.463-464
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• 2009

This study proposes a new rapid-screening method for more reasonably evaluation the seismic capacities of low-rise RC buildings controlled by both shear and flexure. At the same time, this develops the equation of damage judgement and seismic capacity evaluation for quantitatively evaluating the seismic capacities. Using this evaluating method, it is impossible to estimate the evaluation score and earthquake-damage degree confronted with this and evaluate for efficiently the seismic capacities

• Journal of Korean Association for Spatial Structures
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• v.13 no.3
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• pp.57-64
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• 2013

Since the terrorist attacks 9.11 in 2001, the public places such as subway, department stores or office buildings have become a target of terror in major public facilities. In this paper, for the prevention of terrorist attack in domestic building, the assessments of terror risk were conducted and their relationship with building heights was discussed through FEMA 455-Rapid Visual Screening(RVS). Explosive terror risk tends to increase rapidly with building heights(H<230m), showing the correlation coefficient between total risk and building heights(H<230m) is 0.93. In addition, The average of consequences is 8.47 and that of' threat is 8.95. Vulnerability is found to be 6.62 in average.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.118.3-119
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• 2003

Diazepam (DZ) is one of the most frequently prescribed drugs as an antianxiety agent, muscle relaxant, and anticovulsant and sometimes causes intoxication due to accidental overdose, misuse or abuse. Screening or confirmation methods for DZ and NDZ in plasma are very important for clinical and toxicological studies and in forensic cases. GC/MS assay with SPE was developed for the determination of diazepam and its metabolite, nordiazepam in human plasma. Diazepam in plasma was extracted by a rapid and sensitive procedure based on C18 bonded-phase extraction. (omitted)

• Earthquakes and Structures
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• v.16 no.1
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• pp.29-39
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• 2019

A three-step screening process is presented in this article for selection of consistent earthquake records in which number of suitable ground motions is quickly screened and reduced to a handful number. Records that remain at the end of this screening process considerably reduce the dispersion of structural responses. Then, an effective method is presented for spectral matching and modification of the selected records. Dispersion of structural responses is explored using different statistical measures for each scaling procedure. It is shown that the Uniform Design Method, presented in this study for scaling of earthquake records, results in most cases in the least dispersion measure.

• Journal of Radiation Industry
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• v.18 no.2
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• pp.101-107
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• 2024

Rapid screening for internal contamination by alpha- and beta-emitting radionuclides is essential in situations involving radiation workers or radiation accidents. This study focused on the use of urine samples and liquid scintillation counting to quickly and accurately assess contamination. Calibration of the alpha and beta detection areas ensured precise measurement results. The major radionuclides recommended for surveillance during accidents were also considered. This study evaluated the effectiveness of the method by examining various parameters, including the limit of detection, linearity, sensitivity, selectivity, accuracy, ruggedness, and blind test sample analysis. The liquid scintillation counting method is an effective tool for screening urinary samples to detect alpha- and beta-emitting radionuclides, particularly during radiation emergencies, despite some limitations in precision.

• Current Research on Agriculture and Life Sciences
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• v.31 no.1
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• pp.1-10
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• 2013

The exposure of food to ionizing radiation has been recognized as a safe and effective mode of food preservation in more than 55 countries. The benefits include eradication of insect pests, inactivation of food pathogens, extension of shelf-life, and improvement in food hygiene. Regulatory authorities around the world have emphasized the implementation of various national and international regulations to facilitate trade and development of consumers' confidence in purchasing irradiated foods. Therefore, the need for reliable irradiation detection methods has increased to enforce these regulations. At present, a number of promising analytical approaches have been developed and evaluated. Moreover, about 10 European Standards have been adopted as General CODEX Alimentarius methods for the detection of irradiated foodstuffs. However, most of these methods demand relatively expensive equipment and prolonged sample preparation. Therefore, simple and cost-effective approaches would be advantageous for rapid screening of foodstuffs. The suspected samples need to be analyzed further with more validated techniques to confirm the screening results. In this review, existing screening methods (i.e. physical, chemical, and biological) for the identification of irradiated foods have been outlined along with their principles, scopes and limitations.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.232-234
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• 2003

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1117-1123
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• 2019

Control of pine wilt disease, which is caused by pine wilt nematode Bursaphelenchus xylophilus, is heavily dependent on the use of chemicals such as abamectin. Although such chemicals are highly effective, demands for alternatives that are derived preferentially from natural sources, are increasing out of environmental concerns. One of the challenges to discovery of alternative control agents is lack of fast and efficient screening method that can be used in a high-throughput manner. Here we described the development of colorimetric assay for the rapid and accurate screening of candidate nematicidal compounds/biologics targeting B. xylophilus. Contrary to the conventional method, which relies on laborious visual inspection and counting of nematode population under microscope, our method utilizes a redox dye that changes its color in response to metabolic activity of nematode population in a given sample. In this work, we optimized parameters of our colorimetric assay including number of nematodes and amount of redox dye, and tested applicability of our assay for screening of chemicals and biologics. We demonstrated that our colorimetric assay can be applied to rapid and accurate quantification of nematode viability/mortality in a nematode population treated with candidate chemicals/biologics. Application of our method would facilitate high-throughput endeavors aiming at finding environment-friendly control agents for deadly disease of pine trees.