• Journal of Dairy Science and Biotechnology
• /
• v.14 no.1
• /
• pp.71-84
• /
• 1996

This study was conducted to examine the usability of sorbitol for the manufacture as low-calory ingredient and cryoprotectant against frost damage. When frozen yoghrt was made of replacing sucrose by sorbitol at yoghurt mix, the change of physicochemical and lactic acid bacteria, such as Str. thermophilus, L. bulgaricus, and mixed culture of Str. thermophilus, and L. bulgaricus(1:1), was studied during the frozen storage(-20$^{\circ}$C). During the incubation of yoghurt mix, the rapid growth of lactic acid bacteria in all sample was observed as the increase of sorbitol addition, but sample A and D were almost similar. This results suggested that sucrose could play role of effecting the growth stimulator, otherwise, sorbitol could inhibit the death of microorganism, following the genus. At the survival rate between lactic acid bacteria during freezing of -5$^{\circ}$C by ice cream freezer Str. thermophilus showed 26.19 to 34.76%, L. bulgaricus 3.97 to 5.20%, and mixed culture 17.16 to 40.87% respectively. L. bulgaricus showed the greater lethal rate than other genus. Sample C which mixed sucrose with sorbitol (1:2 ratio) was showed the lowest lethal rate. Therefore, it suggested that the use of this ration could be used for better anti-frost damage. During the storage of -20$^{\circ}$C, the number of lactic acid bacteria generally decreased in the stand point of genus and frozen storage period. The survival of lactic acid bacteria might be the addition of sorbitol which could have the effect of anti-forst damage. In all treatment, lactase activity showed the rapid decrease after freezing. During the period of frozen storage, it was shown the slow decreasing trend. In spite· of decreasing, the result during yoghurt mix incubation -5$^{\circ}$C freezing, and -20$^{\circ}$C frozen storage was different at the level. After 80 days of storage, the lactase activity was similar among all genus and sample. Despite differenting viscosity followed by genus, combination of mix, and pH, the ratio of 1 to 2(sucrose : sorbitol) showed the greatest viscosity. The water holding capacity of frozen yoghurt mix was closely related to viscosity. As increasing sorbitol amounts, hardness and cohesiveness were increased, but elastisity was decreased. The significant differences between sample was inoculated with Str. thermophilus. However, there were not significant difference from the sample inoculated with L. bulgaricus and mixed culture.

• Korean journal of applied entomology
• /
• v.60 no.2
• /
• pp.193-199
• /
• 2021

Solenopsis japonica, which is belonging to Formicidae in Hymenoptera, is a native ant species in Korea. However, it had not been studied for cold hardiness of S. japonica to understand on its overwintering mechanisms in field so far. Cold tolerance on developmental stages was measured at different cold temperature with various exposure times. Workers showed more survival at 5℃ and 10℃ compared with other stages and elevated cold tolerance when workers were exposed at 15℃ for more than 12h incubation as a rapid cold hardening (RCH) condition. RCH treatment not only increased survival of workers at cold temperatures, but also decreased supercooling point (SCP) and freezing point (FP). RCH group increased the survival rate by 44% at 10℃ compared with Non-RCH group. SCP and FP were depressed from -10.0 to -14.2℃ and from -11.3 to -15.3℃, respectively, after RCH treatment. Cold temperature increased expression level of cold- and stress-related genes such as glycerol kinase and heat shock protein. These results indicate unacclimated cold tolerance of S. japonica and its acclimation to low temperature by RCH.

• Journal of the Korea Concrete Institute
• /
• v.20 no.3
• /
• pp.375-381
• /
• 2008

Various problems such as durability degradation may happen when extra water is added to concrete. Because of these reasons, the change of water content is managed by using rapid evaluation method of unit water content such as electric capacity method, heat drying method making use of micro wave, unit capacity mass method among various methods. Especially, in Japan, guidance for the change of water content ($\pm$ 10, 15, 20 kg/$m^3$ etc.) were regulated and used. However, it is the real situation that the guidance which were regulated in South Korea evaluate suitability only considering production and measurement error under the circumstances which are not considering the degree of durability degradation. Therefore, this study tries to investigate the influence of addition of extra water in the concrete on the durability degradation of concrete when it was added by artificial manipulation or by management error. From the test results, a guideline of the contents of extra water for the quality control is suggested with the consideration of the degree of durability degradation and the probable error resulted from the addition of extra water. The contents of extra water for tests are set as 0, 15, 25, 35 kg/$m^3$. To examine the durability degradation of concrete, freezing and thawing, carbonation, chloride penetration and compressive strength are tested.

• Korean Journal of Animal Reproduction
• /
• v.24 no.3
• /
• pp.225-230
• /
• 2000

This study was designed to investigate effect of cryoprotectant kinds and cell stages on the viability of bovine embryos cryopreserved by vitrification. The oocytes were collected from ovarian follicles of Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing hormone and 10%(v/v) FCS for 24~48hrs in a incubator with 5% $CO_2$, in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 7~10 hrs with spermatozoa capacitated by preincubation. The vitrification solutions of EFS and EDS were consisted of 40%(v/v) ethylene glycol, 18%(v/v) Ficoll and 0.3M sucrose, and 20%(v/v) ethylene glycol, 16.5%(v/v) DMSO and 0.5M sucrose in TCM-199 medium supplemented with 10% FCS, respectively. The embryos were exposed to EFS or EDS at $25^{\circ}C$ and loaded into OPP straw for 30 sec. The plug end of each straws was heat-sealed and straws was slowly immersed into liquid nitrogen(L$N_2$). The results obtained were summarized as follows : 1 . The rates of cleavage and hatching of embryos frozen with vitrification, rapid and slow freezing methods were 67.5%, 27.5% and 42.5%, 20.0% and 52.5%, 25.0%, respectively And rates of cleavage and hatching of embryos frozen with vitrification method were significantly(p<0.05) higher than those in other methods, and the rates were lower than those in control group(82.5% and 37.5%). 2. The rates of cleavage and hatching of embryos were significantly(p<0.05) different between EFS(47.5% and 22.5%) and EPS(52.5% and 27.5%), and the rates were lower than those in control group(82.5% and 37.5%). 3. After vitrification freezing of bovine embryos at zygote, 2 cell, 8 cell, morulae and blastocyst stage, the rate of cleavage and hatching were 25.0% and 15.0%, 32.5% and 20.0%, 37.5% and 20.0%, 52.5%, 27.5%, 47.5% and 25.0%, respectively. And developmental rates to the expended blastocyst stage of embryos frozen at zygote stage was significantly(p<0.05) lower rather than those in 2, 8-cell and morulae stage.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.19 no.2
• /
• pp.125-132
• /
• 2015

This paper presents an experimental investigation in order to evaluate fresh and hardened properties of LP (Limestone Powder) blended cement concrete. The cement contents of the mixtures are replaced by LP in the range of 10%, 15%, 25%, and 35%, while a control mixture is prepared with only OPC (Ordinary Portland Cement). The fresh concrete properties like slump and air content are similar to those of control mixture up to 35% of replacement ratio of LP, however a delay in setting time is evaluated. The hardened properties including compressive strength, flexural strength, and rapid freezing and thawing resistance shows similar results of control mixture up to 15% of replacement. Relatively lower strength development is evaluated over 25% replacement of LP. For accelerated carbonation test, resistance to carbonation rapidly decreases with increasing LP replacement ratio due to the limited amount of $Ca(OH)_2$. From the study, LP replacement under 15% can be adopted considering reduction of strength and resistance to carbonation.

• Journal of the Korea Institute of Building Construction
• /
• v.16 no.6
• /
• pp.595-601
• /
• 2016

The insulation performance of the insulation currently used in building structures is reflected only during design based on initial performance and the reduction in heat insulation performance due to the degradation of long-term durability is not reflected. This study reviewed the degradation of heat insulation performance due to the durability degradation of insulating materials through the accelerated durability test. The study findings showed that the foamed polystyrene insulation bead method did not show performance degradation due to aging in the standard environmental condition and laboratory accelerated test condition but the performance is degraded in the freeze-thaw test condition. On the other hand, in the case of the extrusion method, the degradation of the heat insulation performance was less in the freeze-thaw test condition, but the rapid performance degradation was caused by the release of the internal gas at the beginning of aging. In addition, the hard polyurethane foam insulation showed better initial insulation performance than other insulation materials, but the performance was found to be degraded somewhat under laboratory accelerated test conditions and freeze-thaw test conditions.

• Molecules and Cells
• /
• v.26 no.6
• /
• pp.558-565
• /
• 2008

Although the fertilizing ability of spermatozoa is greatly reduced after freezing, complete understanding of alterations induced by cryopreservation has not been elucidated. The present study evaluates the effects of cryopreservation on the $Ca^{2+}$ handling of boar spermatozoa using several sperm activators. Intracellular $Ca^{2+}$ signals from single spermatozoa were measured using confocal $Ca^{2+}$ imaging of unfrozen samples and of other spermatozoa after having been frozen. Elevation of the external $K^{2+}$ concentration elicited a three times larger $Ca^{2+}$ increase in fresh spermatozoa than in cryopreserved spermatozoa. Caffeine elicited $Ca^{2+}$ transients with some oscillations in the fresh spermatozoa, but not in the thawed spermatozoa. Depletion of the $Ca^{2+}$ store with thapsigargin induced a rapid rise in $Ca^{2+}$ in the control but generated a smaller increase of $Ca^{2+}$ after thawing. Exposure to progesterone induced a biphasic rise of the $Ca^{2+}$ level in the fresh spermatozoa only. Sperm viability was reduced by cryopreservation. Resting $Ca^{2+}$ levels in fresh and cryopreserved spermatozoa were similar. Longer incubation (2.5 h) of thawed spermatozoa partly recovered the $Ca^{2+}$ response to the interventions. These results suggest that cryopreservation reduces the responsiveness of spermatozoa to depolarization, modulators of the internal $Ca^{2+}$ store and progesterone in terms of the $Ca^{2+}$ signal, thus providing a possible mechanism for reduced fertility observed in cryopreserved boar spermatozoa.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.13 no.3
• /
• pp.13-24
• /
• 1993

The influence of the bubble structure and strength characteristics on the freeze-thaw resistance of high strength concrete is investigated by the laboratory experiment. The test conditions are formed in the manner that water is continueusly supplied externally and the specimens were received severe weather actions from ordinary to significantly low temperatures. The experiments are performed in two stages. In the first stage, the relation between the durability to frost action and the bubble structure is analyzed especially with respect to the water-cement ratio and the amount of air. The AE and non-AE concrete specimens made of ordinary portland cement are used in the test. In the second stage, the non-AE concrete specimens using vibratory compaction to improve the durability to frost action, and the high watertight specimens of rapid hardening portland cement to increase their initial strength are produced and tested. The degree of watertightness of the specimens is determined by measuring the permeability of the specimens and the bubble structure of the high watertight concrete is also estimated.

• Journal of the Korea Concrete Institute
• /
• v.21 no.5
• /
• pp.639-647
• /
• 2009

In order to evaluate the durability of reinforced concrete structures under chloride attack from sea water and frost damage, it is important to analyze both the microstructural characteristics of concrete and its diffusion resistance of concrete against chloride ingress. In this study, a relation between micro-pore structures of concrete obtained by the Mercury Intrusion Porosimetry and accelerated chloride diffusivity as well as long term chloride diffusivity were studied for ground granulated blast furnace slag(GGBS) concrete. Different water-cement ratio of 40, 45, 50% and different unit cement concrete of 300, 350, 400 or 450 kg/$m^3$ of the GGBS concrete along with OPC concrete were used and freeze and thawing, and the change in diffusivity and microstructure were observed for both GGBS concrete and damaged GGBS concrete due to rapid freezing and thawing.

• Proceedings of the KSAR Conference
• /
• 2001.10a
• /
• pp.57-57
• /
• 2001

This study was carried out to investigate the general characteristics such as volume, sperm concentration, sperm motility, sperm abnormality on whole semen, RSP-S and RSP-T semen and fractional semen of small size dogs, and the effect of temperature and preservation time and cryoproservation on motility of whole and RSP-S and RSP- T semen. Multiple ejaculates were collected from small dogs by the digital manipulation of penis. 1. The volume per ejaculate semen, sperm of concentration and motility and abnormal sperm rate of 1st fractional semen were 0.65±0.09㎖, 4.52±0.35×10/sup 6/ cells/㎖, 15.64±3.85% and 5.50±0.62%. Also, 2nd fractional semen were 1.25±0.20㎖, 3.35±0.48×10/sup 6/cells/㎖, 96.25±4.65% and 4.24±0.46%. And 3rd fractional semen were 1.45±0.21㎖, 3.85±0.52×10/sup 6/cell/㎖, 92.82±4.24% and 4.66±0.58%, respectively. 2. The sperm of concentration and motility and abnormal sperm rates of whole, RSP-S and RSP-T semen were 5.45±0.82×10/sup 6/ cells/㎖, 95.55±4.65%, 4.58±0.45% and 4.82±0.36×10/sup 6/cells/㎖, 90.10±3.42%, 6.48±0.68% and 4.55±0.45× 10/sup 6/cells/㎖, 93.25±3.85%, 4.82±0.58%, respectively. 3. The motility of whole, RSP-S and RSP-T semen were higher at 4℃ than at 38℃. When preservation temperature was at 4℃, survival rates of RSP-S and RSP-T sperm were 97.54%-6.25% at 1-72 hrs, 97.40%-5.62% at 1-100 hrs, respectively. 4. The survival rates of slow and rapid frozen 2nd fraction, RSP-S and RSP-T semen were 67.3±4.45%, 88.8±4.46% and 46.4±3.84%, 74.4±4.20%, respectively. Survival rates was significantly higher in frozen RSP-S and RSP-T semen than that in control group(8.5±2.12%).