• Proceedings of the Korean Magnetic Resonance Society Conference
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• 2002.01a
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• pp.9-9
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• 2002

• Animal cells and systems
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• v.4 no.1
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• pp.45-50
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• 2000

We found a brown frog species, which is unrecorded from South Korea. Rana huanrenensis Fei, Ye, and Huang (1990), This species was originally described from northeastern China. In having 2n=24 chromosomes, this species is closely related to Rana dybowskii, R. chensinensis, R. ornativentris, R. pirica, and Chinese R. huanrenensis, but it is different from the first four species in the ecological, morphological, and genetic characteristics. By contrast, this species Is identical to Chinese R. huanrenensis In the habitat of montane stream-breeding, absence of the vocal sac, and genetic properties. This record is a significant range extention of R. huanrenensis.

• Proceedings of the Zoological Society Korea Conference
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• 1998.04a
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• pp.32-32
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• 1998

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.119.1-119
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• 1997

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.43.2-43
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• 1999

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.45.1-45
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• 1999

No Abstract, See Full Text

• Applied Microscopy
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• v.35 no.4
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• pp.32-41
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• 2005

The sperm morphology of two Korean frogs, Rana catesbeiana and R. nigromaculata (Ranidae) was studied with the light microscope, scanning and transmission electron microscopes. Sperm heads of the R. catesbeiana and R. nigromaculata were long-cylinder shapes. In the total length of R. nigromaculata sperm ($51.2{\mu}m$) was longer than those of R. catesbeiana ($44.6{\mu}m$). Both R. catesbeiana and R. nigromaculata had the long head and principal piece, and short middle piece, but the neck was undeveloped. The axonemal structures contain a 9+9+2 arrangement of microtubules. The number of mitochondria in middle piece have less than mammalian sperm, which irregular surrounded the axone of the middle piece. In conclusion, R. catesbeiana and R. nigromaculata shared many similar features in sperm heads, neck, middle piece, and a site of mitochondria and axial structure, and it suggests that these species should be closely related.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.66-74
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• 2004

In order to clarify intra-and inter-specific variation of Korean Rana species, the partial DNA sequences of mitochondrial 16S rDNA gene were determined from 6 Korean and 1 Japanese Rana species, DNA sequences from Korean and Japanese species were comparison-analysed within, and also with the sequences from three species of Japanese brown frogs. DNA similarities were calculated as 91.3∼97.3％ among brown frog (R. amurensis coreana, R. dybowskii and R. huanrenensis), as 96.11∼97.26％ among pond frogs (R. nigromaculata and R. planeyi chosenica). Genetic distance of pond frog and wrinkle fyog (R. rugosa) were near than that of pond frog and brown frog. Two clusters were formed brown frogs and the other group by neigh-bor-joining and maximum-likelihood analysis, also the populations of R. nigromaculata were well distinguished between Korean peninsula and Korean island. But result from maximum-likelihood analysis slightly differed from neighbor-joining to cluster of R. rugosa. Further analyses for their population will be necessary to study the phylogenetic status.

• Animal cells and systems
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• v.4 no.2
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• pp.117-121
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• 2000

Genetic relationships among six species of the genus Rana from Korea were investigated by complete nucleotide sequence analyses of mitochondrial cytochrome b gene (1143 bp). Based on Kimura-2-parameter distance, the interspecific sequence differences of cytochrome b gene within the genus Rana were ranged from 7.83% to 25.00%. The genetic distances were 7.83% between R. nigromaculata and R. plancyi, 8.47% between two types of R. rugosa (type A and B), 10.42% between the brown frogs (R. amurensis and R. dybowskii), 16.11% between R. dybowskii types 1 and 2 and 12.36% between pond frogs (R. nigromaculata and R. plancyi) and R. catesbeiana. In the neighbor-joining and parsimony trees, R. catesbeiana was more closely related to pond frogs than brown frogs. R. dybowskii types 1 and 2 were considered to be at a distinct and specific level of differentiation (16.11%), while two types of R. rugosa were suspected to be at a subspecific level (8.47%).

• Animal cells and systems
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• v.4 no.1
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• pp.23-30
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• 2000

Horizontal starch gel electrophoresis for 29 populations (n=543) of the wrinkled frog, Rana rugosa, from Korea and Japan was peformed to assess the degree of genic variation and genetic diversity, and to understand the biogeographic pattern of distribution and speciation. A sum of 22 presumptive loci was screened from 17 enzymes and general proteins. Four loci, Aco, Est-3, Me-2, and Pgm, demonstrated high levels of polymorphism. The degree of average genetic variation of R. rugosa was P=22.7% (9.1-40.9%), Ho=0.086 (0.048-0.165) and He=0.090 (0.042-0.168). In the south-eastern region of the Korean peninsula (Chongsong, Yongchon, Ulsan, Kyongju, Pohang, yongdok and Ulchin), a few unique alleles in the Mpi locus were detected and their biogeographic implications were considered. The degree of genetic differentiation among the Korean populations was moderate (S=0.900), whereas the degree of genetic diversity between Korean and Japanese populations was notably high (S=0.687, D=0.293). This result corresponds with the data obtained by the mitochondrial cytochrome b gene sequence (Lee et al., 1999) suggesting that the Korean and Japanese R. rugosa might have evolved a specific level of genetic differentiation since their geographic isolation.